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Whey protein antihypertensive peptide prepared by utilizing continuous enzyme membrane reactor and special device thereof

An enzyme membrane reactor and whey protein technology, applied in the field of whey protein blood pressure-lowering peptide and its special device, can solve the problems of unable to realize simultaneous proteolysis and separation of active peptide, unable to recycle, difficult to continuous production, etc. , to achieve the effect of eliminating the inhibition of the product on the enzyme, saving the production cost and having strong stability

Active Publication Date: 2010-06-09
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A review of domestic and foreign patents and literature shows that there are already public patents on the use of step-by-step enzymatic hydrolysis technology to enzymatically hydrolyze casein, alfalfa protein, soybean protein, etc. to prepare active peptides. And can not be recycled, difficult to continuous production
However, the production device and process for producing whey protein blood pressure-lowering peptides by using continuous enzyme membrane bioreactor to hydrolyze whey protein and continuously feeding have not been reported yet.

Method used

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  • Whey protein antihypertensive peptide prepared by utilizing continuous enzyme membrane reactor and special device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, preparation whey protein antihypertensive peptide

[0032] Prepare a concentrated whey protein solution with a mass concentration of 2%, and after hydration (hydration conditions: adjust the pH of the WPC solution to 7.5, then hydrate and continuously stir for 20 minutes at 85°C) to the continuous enzyme membrane reactor Add 2L of WPC solution to the reaction tank 4, control the temperature of the reaction tank to 40°C, and then add 80kU alkaline protease (purchased from Beijing Fangshan Enzyme General Factory) to the reaction tank 4 according to the ratio of 2000U protease / g whey protein Carry out enzymolysis reaction; Open valve 11,18, utilize peristaltic pump 12 to regulate circulation flow rate to be 12.5L / h, close valve 14 now, utilize valve 18 to regulate transmembrane pressure to be 0.02MPa (the pressure that pressure gauge 17 shows), The enzymatic hydrolysis reaction solution enters the ultrafiltration membrane module 3, the ultrafiltration membran...

Embodiment 2

[0038] Embodiment 2, preparation whey protein antihypertensive peptide

[0039] Select a concentrated whey protein solution with a mass concentration of 5%, after hydration, take 2L and add it to the reaction tank of the continuous enzyme membrane reactor, control the temperature at 50°C, and add 200kU of alkali according to the ratio of 2000U protease / g whey protein protease (purchased from Beijing Fangshan Enzyme General Factory); the circulating flow rate is 34.2L / h, the transmembrane pressure is adjusted to be 0.08MPa, the molecular weight cut-off of the ultrafiltration membrane is 10000Da, and the ultrafiltration process is continuously fed (the feeding concentration is 2.5 %). After 10 hours of continuous enzymatic hydrolysis, the change rate of the membrane flux was -4.58%~+3.78%, which was relatively stable. The protein recovery rate was 81.43%, and the ACE inhibition rate of the obtained whey protein blood pressure-lowering peptide was 83.18%. The cleaning method ad...

Embodiment 3

[0040] Embodiment 3, preparation whey protein antihypertensive peptide

[0041] Select the whey protein isolate solution with a mass concentration of 8%, get 2L and add it to the reaction tank of the continuous enzyme membrane reactor after hydration, add 320kU alkaline protease (purchased in Beijing City Fangshan Enzyme General Factory); Circulation velocity is at 44.2L / h, and regulation transmembrane pressure is 0.08MPa, and ultrafiltration membrane molecular weight cut-off is 10000Da, and ultrafiltration process is constantly fed (feed concentration is 3%). After continuous enzymatic hydrolysis for 10 hours, the change rate of the membrane flux was -11.82~+11.91%, which was relatively stable. The protein recovery rate was 85.21%, and the ACE inhibition rate of the obtained whey protein-derived hypotensive peptide was 81.08%. The cleaning method adopts the cleaning method in Example 1.

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Abstract

The invention discloses a whey protein antihypertensive peptide prepared by utilizing a continuous enzyme membrane reactor and a special device thereof. The continuous enzyme membrane reactor provided by the invention comprises a reactive tank (4), an ultrafiltration membrane assembly (5), a circulating system (7), a collecting tank (9) and a feed tank (6), wherein the reactive tank (4) is connected with the ultrafiltration membrane assembly (5) through the circulating system (7), the feed tank (6) is connected with the reactive tank (4) through a pipeline, and a peristaltic pump (10) is arranged on the pipeline. After whey protein is preprocessed through dissolution, hydration and the like, the antihypertensive peptide is prepared in the continuous enzyme membrane reactor by adopting thetechnologies of proteolysis and membrane separation and coupling. The enzyme membrane reactor shows excellent stability in the operating process, and the ultrafiltration amount thereof always keeps more than 90 percent after the enzyme membrane reactor continuously works for 12 hours. The protein recovery rate reaches more than 78 percent, and the highest suppression ratio of an ACE can reach 90.11 percent.

Description

technical field [0001] The invention relates to a whey protein hypotensive peptide prepared by a continuous enzyme membrane reactor and a special device thereof. Background technique [0002] At present, there are nearly 1 billion people in the world suffering from high blood pressure, and another 500 million people will join the ranks of this silent killer by 2025. Hypertension is the most harmful chronic disease in the 21st century. This so-called "rich man's disease" is no longer a "patent" of Western countries, and even some African countries with relatively backward economies have become "epidemic areas" of high blood pressure. Today, millions of people die each year from heart disease caused by high blood pressure. [0003] Extracting polypeptides with nutritional and physiological activities from natural proteins and applying them to health food and food fortified nutrition can not only make full use of high-quality protein in food, but also increase the added value ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/40C12M1/12C12P21/06C07K14/76A23L1/305A23L33/19
CPCC12M25/02
Inventor 毛学英陈耿华王磊
Owner CHINA AGRI UNIV
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