The invention discloses an anti-spot beautifying composition comprising the raw materials of skin whitening and moisturizing components of: golden thread, ethanol, lightyellow sophora root, rhubarb, cape jasmine, ligusticum, Szechwan lovage rhizome, largehead atractylodes rhizome, redroot gromwell, ginseng, common bletilla tuber, and the like. A preparation method of the composition comprises the steps that: the materials are sieved and placed in a soaking tank; the materials are mixed with ethanol, and the mixture is stirred; heating and continuous soaking are carried out; the obtained material is filtered; the filtrate is subjected to heating and reflux extraction, and an obtained filtrate is preserved for later use; ligusticum and Szechwan lovage rhizome are soaked by using water, and volatile oil is collected by distillation; the volatile oil is preserved for later use; and the filtrate, the volatile oil, auxiliary materials, arbutin, panthenol, and water are blended, such that a finished product is obtained. The composition provided by the invention causes no irritation to skins, and assists in improving skin capillary permeability and promoting skin blood circulation. The composition has a substantial inhibiting effect against intracellular tyrosinase activity, and assists in substantially reducing intracellular melanin content. With the composition, epidermal cell regeneration capacity development can be accelerated, and skin aging can be retarded. For malar rash, chloasma, dark spots, acne scars, and the like, functions of whitening and spot removing can be achieved rapidly.