Novel microbial organic fertilizer for preventing and treating perennial root dwarf disease of Saccharum officinarum and preparation method therefor

A disease prevention and organic fertilizer technology, applied in the field of new microbial organic fertilizer and its preparation, can solve the problems of biological organic fertilizer, sugarcane bud death, and poor detoxification effect that have not yet been found in sugarcane perennial root dwarf disease, and achieve large-scale application value, control the incidence of disease, and increase the effect of yield

Inactive Publication Date: 2016-08-17
GUANGZHOU YOURUI BIOSCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the temperature of soaking in warm water is usually 50-52°C, which is very close to the critical lethal temperature of sugarcane buds. In addition, the temperature that old buds and young buds can withstand is different. If the temperature is too low, the detoxification effect will be poor, and if the temperature is too high, It is easy to cause the death of sugarcane buds, and the shoot tip detoxification culture technology needs to take a shoot tip size of 0.1-0.2mm, which requires sophisticated instruments and relatively high requirements for operators, which limits the application of this method.
So far, in the prior art, no research reports on bio-organic fertilizers for the prevention and treatment of sugarcane perennial dwarf disease have been found.

Method used

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  • Novel microbial organic fertilizer for preventing and treating perennial root dwarf disease of Saccharum officinarum and preparation method therefor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of decomposed mixed bacteria: respectively inoculate the activated Bacillus subtilis and Bacillus thermophilic licheniformis into the culture medium of the Erlenmeyer flask, the inoculum amount is 3-5% (V / V), inoculate into the fermenter after 12 hours of cultivation Expand the culture medium until the number of viable bacteria reaches 10 8 cfu / mL to get Bacillus subtilis culture fluid and thermophilic Bacillus licheniformis culture fluid;

[0040] Among them, the medium components of Bacillus subtilis are based on mass percentage content: glucose 6%, yeast extract 1%, peptone 0.2%, CaCO 3 1%, the rest is water; culture temperature is 28-32℃;

[0041] The medium components of the thermophilic Bacillus licheniformis culture medium are calculated by mass percentage: 0.5% yeast extract, 0.5% peptone, 5% brown sugar, 0.1% Tween-80, 0.05% sodium chloride, 0.05% dihydrogen phosphate Potassium, 0.05% magnesium sulfate, 0.05% calcium carbonate, the rest is w...

Embodiment 2

[0052] (1) Preparation of decomposed mixed bacteria: use the steps in the example (1) to expand culture to obtain Bacillus subtilis culture fluid and thermophilic Bacillus licheniformis culture fluid; Mixed at 1.5:1 to obtain decomposed mixed bacteria;

[0053] (2) Preparation of feces straw decomposed clinker: mix pig manure and wheat straw powder evenly at a mass ratio of 3:1 to obtain fermentation raw materials; add decomposed mixed bacteria with an inoculum amount of 8% (g / g) to the fermentation raw materials , adjust the moisture content to 50%, loosely place it in the fermentation tank, and ferment under the condition of 40-45 ° C. During the fermentation process, turn the pile once every 3 days, and ferment for 15 days until it reaches maturity, that is, the decomposed clinker;

[0054] (3) Pathogen-inhibiting bacteria culture solution for sugarcane perennial dwarf disease: expand the activated Bacillus thuringiensis according to the method in step (3) of Example 1, and...

Embodiment 3

[0059] (1) Preparation of decomposed mixed bacteria: use the steps in the example (1) to expand culture to obtain Bacillus subtilis culture fluid and thermophilic Bacillus licheniformis culture fluid; Mixed at 2.5:1 to obtain decomposed mixed bacteria;

[0060] (2) Preparation of feces straw decomposed clinker: mix pig manure and wheat straw powder evenly at a mass ratio of 2.5:1 to obtain fermentation raw materials; add decomposed mixed bacteria with an inoculum amount of 12% (g / g) to the fermentation raw materials , adjust the moisture content to 53%, loosely place it in the fermentation tank, and ferment it under the heat preservation condition of 50-55°C. During the fermentation process, turn the pile once every 3 days, and ferment for 11 days until it reaches maturity to obtain the decomposed clinker;

[0061] (3) Pathogen-inhibiting bacteria culture solution for sugarcane perennial dwarf disease: expand the activated Bacillus thuringiensis according to the method in step...

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Abstract

The invention discloses a novel microbial organic fertilizer for preventing and treating perennial root dwarf disease of Saccharum officinarum. The novel microbial organic fertilizer is prepared through the following steps: mixing animal wastes and straw powder, inoculating well-composted mixed bacteria to the mixture, and carrying out compost fermentation, so as to obtain a well-composted material; carrying out amplification culture on bacillus thuringiensis so as to obtain a culture solution for pathogen inhibiting bacteria for perennial root dwarf disease of Saccharum officinarum; carrying out amplification culture on streptomyces globisporus and bacillus megaterium, and carrying out proportioning mixing, so as to obtain an effect promoting bacterium mixed solution; proportionally mixing bamboo charcoal, diatomite and zeolite powder, so as to obtain a soil amendment; and uniformly mixing the well-composted material, the culture solution for the pathogen inhibiting bacteria for the perennial root dwarf disease of the Saccharum officinarum, the effect promoting bacterium mixed solution and the soil amendment proportionally, and carrying out drying, crushing, sieving and granulating, thereby obtaining the microbial organic fertilizer. The microbial organic fertilizer provided by the invention is high in fertility, can be used for truly effectively inhibiting the incidence of the perennial root dwarf disease of the Saccharum officinarum and can also be used for amending soil, improving the activity of the soil, increasing the yield of the Saccharum officinarum and shortening the ripening period of the Saccharum officinarum, thereby having very high economic and social benefits.

Description

technical field [0001] The invention relates to the field of fermented organic fertilizers, in particular to a novel microbial organic fertilizer used for the prevention and treatment of dwarfing diseases of sugarcane perennial roots and a preparation method thereof. Background technique [0002] my country is a large agricultural country, with arable land area of ​​about 2.03 billion mu, ranking fourth in the world. As we all know, the development of agricultural production is inseparable from fertilizers, but in recent years, due to excessive agricultural production and unscientific use of chemical fertilizers in my country, the organic matter and medium and trace elements in the soil are seriously deficient, resulting in soil compaction, a decline in fertilizer utilization, and a decline in the quality of agricultural products. At the same time, environmental pollution and high incidence of pests and diseases have also appeared. [0003] At present, the fertilizers on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/00C05G3/04C05G3/80
CPCC05F3/00C05G3/00C05G3/80C05F11/00C05F11/08C05F17/00
Inventor 刘小阳刘立国
Owner GUANGZHOU YOURUI BIOSCI
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