30 results about "Streptomyces globisporus" patented technology

The invention discloses a compound microbial-fermented organic fertilizer. The compound microbial-fermented organic fertilizer is prepared through the following steps: mixing pig manure and straw powder, inoculating well-composted mixed bacteria to the mixture, and carrying out compost fermentation, so as to obtain a well-composted material; carrying out amplification culture on bacillus thuringiensis so as to obtain a culture solution for pathogen inhibiting bacteria for perennial root dwarf disease of Saccharum officinarum; carrying out amplification culture on streptomyces globisporus, actinomycetes and brown spherical azotobacteria, and carrying out proportioning mixing, so as to obtain a plant probiotics mixed solution; proportionally mixing bamboo charcoal, diatomite and zeolite powder, so as to obtain a soil amendment; and uniformly mixing the well-composted material, the culture solution for the pathogen inhibiting bacteria for the perennial root dwarf disease of the Saccharum officinarum, the plant probiotics mixed solution and the soil amendment proportionally, and carrying out drying, crushing, sieving and granulating, thereby obtaining the microbial-fermented organic fertilizer. The organic fertilizer provided by the invention is high in fertility, can be used for truly effectively inhibiting the incidence of the perennial root dwarf disease of the Saccharum officinarum and can also be used for amending soil, improving the activity of the soil, increasing the yield of the Saccharum officinarum and shortening the ripening period of the Saccharum officinarum, thereby having very high economic and social benefits.

The invention discloses a biocontrol streptomyces for preventing and treating camellia oleifera diseases and its application. A bacterial strain of the invention is streptomyces globisporus supsp.globisporus CSUFTAC0906002 and the preservation number is CCTCC M 2011012. The method comprises that: mixing fermentation broth of the bacterial strain with rice bran, naturally air-drying and grinding to produce raw powder; adding 40wt% of raw powder, 38wt% of kaolin, 10wt% of sodium lignosulfonate, 7wt% of SDS and 5 wt% of humic acid for preparing inocula; diluting inocula for directly applied to prevent and treat camellia oleifera diseases by a sprinkling and pouring root mode. The bacterial strain has preventive and therapeutic effect on camellia oleifera diseases, and has an antagonistic action on a plurality of pathogenic fungi. The bacterial strain has the advantages of various action modes and rapid breeding speed to the pathogen, especially has strong inhibiting effect on camellia anthracnose. The invention has important significance on preventing and treating camellia anthracnose, which has good application prospect for developing.

A streptomyces globisporus H50 (CCTCC No.M203034) for preventing and eliminating the hypochnus and blast of rice is obtained from soil by screening with PAD and YPTG plate method. Its advantage is high effect (more than 65% for its bacterium inhibiting rate).

The invention discloses endophytic actinomycetes of kiwifruit roots and an application of the endophytic actinomycetes of the kiwifruit roots. The endophytic actinomycetes of the kiwifruit roots are Streptomyces globisporus and named as Streptomyces globisporus SCAU-1, and the collection number of the Streptomyces globisporus SCAU-1 is CCTCC NO: M 2016577. According to the invention, functional endophytic actinomycetes are separated from roots of kiwifruit plants suffering from cankers, thereby promoting plant growth, further being capable of obviously inhibiting the growing abilities of plant pathogenic fungi and pathogenic bacteria, and being capable of being used for preparing a fertilizer-pesticide combined type microbial fertilizer to promote the growth of kiwifruits; three continuous pot experiments show that SCAU-1 fermentation liquor has an obvious growth-promoting effect for kiwifruit seedlings by using a root-irrigation way after being diluted by clear water, so that the root numbers, root lengths, plant heights, leaf numbers and leaf areas during the kiwifruit seedling stage can be obviously improved.

The invention discloses a streptomyces strain and a combined application thereof in the prevention and treatment of tomato blight and relates to the technical field of microbes. According to the streptomyces strain and the combined application thereof, actinomycetes are obtained through collecting 10-15cm deep-layer soil samples from saline-alkaline land of a suburban district of Tai'an in Shandong and separating by adopting an actinomycete separation technology, and are named streptomyces rimosus M527 and streptomyces globisporus M927 through microbial taxonomic identification, S. rimosus M527 has been collected in CCTCC (China Center for Type Culture Collection) on June 19, 2013 and has the collection number of CCTCC NO: M 2013270, and S. globisporus M927 has been collected in CCTCC on December 25, 2013 and has the collection number of CCTCC NO: M 2013709. Both strains can be fermented to generate active substances which have relatively strong antagonism on Fusarium oxysporum f. sp. Lycopersici Snyder et Hansen and can be used for preventing and treating plant diseases, such as tomato blight. When fermentation products of the two strains are mixed according to the weight percent of (4: 1) to (7: 3), the biological prevention effect is particularly remarkable compared with that when the two kinds of metabolic products are used respectively as single preparations.

The invention discloses a biological agent for controlling a tobacco brown spot. The production strain of the biological agent is sorted and named as Streptomyces globisporus AM6 and the preserving number thereof is CGMCCNo.1701. The prescriptions of the solid and the liquid mediums of the strain are as follows: (1) the incline medium of a test tube is as follows: 200g of fresh potatoes, 20g of dextrose, 17 to 20g of agar, 1000ml of distilled water as well as a natural pH; (2) the prescription of a liquid fermentation medium is as follows: 10.0g of bean powder, 20.0g of cane sugar, 2g of dipotassium hydrogen phosphate, 0.02g of green copperas, 0.5g of calcium carbonate, 0.05g of bitter salt, 1000ml of tap water as well as a natural pH. The invention is prepared into the biological agent by the test tube culturing of the strain and the fermentation culturing of a table concentrator. Test results show that the biological agent provided by the invention has excellent controlling effects on the tobacco brown spot.

The invention belongs to the technical field of agro-microbiology, and in particular relates to the isolation and identification of a biological control streptomyces globisporus strain and the application of the biological control streptomyces globisporus strain in prevention and control of the penicilliosis of citrus. In the invention, the streptomyces globisporus strain JK-1 capable of producing antifungal volatile substances is obtained, is streptomyces globisporus and is preserved in China Center for Type Culture Collection with a preservation number of CCTCC NO: M2010093. The invention also provides microbial agents prepared from the streptomyces globisporus strain JK-1. The volatile substances produced by the streptomyces globisporus strain JK-1 are utilized to prevent and control the penicilliosis of the citrus in preservation and freshness, which indicates that microorganisms and the agents thereof belong to novel biological control agents.

The invention discloses a compound microbial source pesticide for effectively preventing and treating diseases of oil-tea trees and an application method, and in particular relates to a microbial pesticide prepared by actinomycetes globisporus and streptomyces globisporus subspecies CSUFTAC0906002 (preserving number: CCTCC NO: M2011012) and bacterial bacillus brevis Z26 (preserving number: CCTCC NO: M2011013) compounded with other assistants. The invention belongs to the technical field of forest protection. The compound microbial source pesticide can effectively inhibit growth and reproduction of main pathogenic bacteria of the oil-tea trees, and meanwhile, the main components are good in safety to the environment and have good development application prospect.

Belonging to the field of the research and development of microbial medicines, the present invention relates to a preparation method for extracting a novel glycoside hydrolase inhibitor from the fermentation liquor of Streptomyces globisporus. The method has the following characteristics: the fermentation liquor of Streptomyces globisporus is sterilized with high temperature, ultrafiltered and nanofiltered; the concentrated solution is absorbed and eluted by macroporous absorption resin; and after depressurization and concentration, the active component is frozen and dried, so that the glycoside hydrolase inhibitor is prepared. The method has the following advantages: the extraction technique is simple; the extraction process does not need acid and alkali; environment can be protected; and moreover, the extracted glycoside hydrolase inhibitor has inhibition activity on both pancreatic Alpha-amylase and Alpha-glucosaccharase and can be used for the development of blood sugar-decreasing products and weight-losing products.

The invention provides a root dipping microorganism preparation for transplantation of vegetables. The microorganism preparation consists of the following raw materials: 600kg of biogas liquid, 200kg of streptomyces fermentation liquor, 100kg of geophious candida fermentation liquor, 50kg of starch grafted sodium acrylate, 200g of pimacol and 15kg of dipotassium phosphate. The microorganism preparation disclosed by the invention is simple in preparation method; during root dipping for the transplantation of vegetables, the microorganism preparation can be used for enhancing the adverse resistance of vegetable seedlings, promoting root extension and accelerating seedling recovering speed, so that the seedlings are even and strong and the survival rate of transplantation is improved.

The invention relates to a method for preparing a targeted lidamycin production strain by utilizing a gene disruption strain. The method comprises a step of introducing an expression plasmid of a fusion gene, which contains lidamycin apoprotein gene and a fusion gene consisting of different tumor targeted molecular coding sequences, into a lidamycin apoprotein gene disruption strain to obtain the lidamycin production strain with the target corresponding to the target of the tumor targeted molecule, wherein the gene interruption strain is a Streptomyces globisporus C-1027 lidamycin apoprotein gene (cagA) interruption strain, namely S.globisporus AKO; and the tumor targeted molecule includes, but not limited to tumor targeted antibody, tumor targeted peptide, cell factor and the like. The invention also relates to application of the production strain prepared by the method in producing targeted lidamycin.

The invention provides a novel compound, the compound is E-13-(2-methylphenyl)-12-ene-tridecanoic acid, and the compound is derived from streptomyces globiformis C-1027, can influence uptake on low density lipoprotein by cells, and participates in an intracellular lipid metabolism process. The compound is white amorphous powder, is insoluble in water, and can be easily dissolved in acetone, chloroform and other low-polarity organic solvents. The invention also provides a method for preparing the compound by using the regulatory protein SGL6295 and the regulatory protein SGL6296.

The invention discloses a method for disinfecting shrimp eggs without affecting hatchability, which comprises the steps of loosening eggs, preparing a disinfectant, preparing disinfectant liquid and disinfecting, wherein the disinfectant is prepared from the following components in parts by weight: 3 to 8 parts of streptomyces globisporus fermentation liquor, 4 to 7 parts of crab shell extract, 5 to 10 parts of limonene, 1 to 3 parts of beta-phellandrene, 2 to 5 parts of caryophyllene and 3 to 6 parts of citric acid. The method has the following beneficial effects: the disinfectant used in the disinfection method disclosed by the invention is highly toxic to pathogens, and a small amount of disinfectants can take a very good effect, the shrimp eggs are well tolerant to the disinfectant, the pathogens are killed without affecting the hatchability, the problems of low survival rate and poor quality in a hatching process is effectively solved, the disinfection step is simple, the operation is convenient, the practicability is high and good economic benefits are realized.

The invention provides a complete-element biological organic fertilizer special for blumea balsamifera and a preparation method and a using method thereof. The fertilizer comprises a nitrogen-phosphorus-potassium mixed fertilizer and a bacteria solution containing streptomyces globisporus actinomycetes. The preparation method specifically comprises the steps of taking wheat grains and Chinese gooseberry roots as raw materials, washing, sterilizing and fermenting to obtain a culture medium containing the wheat grains or the Chinese gooseberry roots, inoculating a disease-resistant fasciation-type actinomycetes liquid cultural substance into the culture medium, culturing strains till a logarithmic phase, then mixing with clean water fully for diluting, enabling the active bacteria number in 1 ml of solution to be larger than or equal to 10<9>, and obtaining the actinomycetes bacteria solution; mixing a tobacco compound fertilizer, a vinasse compound fertilizer and the actinomycetes bacteria solution to form the complete-element biological organic fertilizer. The complete-element biological organic fertilizer prepared in the invention includes the following components in weight percentage: 10 to 15% of inorganic nutrients, 2 to 3% of amino acid, 40 to 45% of organic matter and 25% of water, wherein the content of the actinomycetes is 2*10<7>, and the pH is 6 to 8.

The invention relates to the technical field of microorganisms, and particularly discloses phosphate-solubilizing bacteria, a microbial agent and application of the phosphate-solubilizing bacteria and the microbial agent. The strain name of the phosphate-solubilizing bacteria is streptomyces ISA-C7, the classification name of the phosphate-solubilizing bacteria is streptomyces globisporus subsp. Globisporus, the phosphate-solubilizing bacteria are preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.23445, the preservation date is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The Streptomyces sp. ISA-C7 has strong phosphate solubilizing ability in both a low-phosphorus environment and a high-phosphorus environment. Streptomyces ISA-C7 is developed into a microbial inoculum, is applied to plant fertilizers, and has important significance for effectively improving the content of rapidly available phosphorus in soil and the utilization rate of a phosphate fertilizer.