Streptomyces coccidioides bacterial agent for preventing and treating tobacco red spot and preparation method thereof

A technology for tobacco red spot disease and Streptomyces coccidioides, applied in botany equipment and methods, microbial-based methods, biochemical equipment and methods, etc., can solve the problem of lack of high-efficiency and low-toxicity chemical control agents, polluted water sources, use restrictions, etc. question

Active Publication Date: 2011-12-28
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tobacco red spot disease is a typical airborne disease. At present, agricultural measures such as health care cultivation and large doses of chemical pesticides are mainly used for prevention and control in China. There is a lack of resistant varieties for regional cultivation; Prevention and control, the cost of medication is increasing year by year, and the effect of prevention and control is gradually weakening. Some pathogenic fungi have developed drug resistance, and the difficulty of prevention and control has also increased.
[0004] Because chemical agents are very easy to cause phytotoxicity, they are also easily washed away by rainwater when applying pesticides, and there is a lack of high-efficiency and low-toxicity chemical control agents. Continuous use of pesticides will easily cause pesticide residues, which will have a bad impact on the environment, pollute water sources, and cause human and animal poisoning. , death, and at the same time cause pathogenic bacteria to develop drug resistance, resulting in certain restrictions on their use
On the other hand, tobacco red spot disease is a disease of tobacco maturity stage. Due to the higher and higher requirements for tobacco safety, higher requirements are put forward for tobacco cultivation itself, especially for tobacco red spot disease in the mature stage. It is not suitable to use residual chemical agents for disease control

Method used

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  • Streptomyces coccidioides bacterial agent for preventing and treating tobacco red spot and preparation method thereof
  • Streptomyces coccidioides bacterial agent for preventing and treating tobacco red spot and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] ——Preparation Method 1 of Liquid Fermentation Inoculant

[0032] (1) Strain preparation

[0033] Streptomyces globisporus (Streptomyces globisporus) AM6 strain was streak-inoculated on the test tube slant medium (200g of fresh potatoes, 20g of glucose, 17-20g of agar, 1000ml of distilled water, natural pH.), and cultivated at 28°C for 4-5 days , to obtain test tube species.

[0034] (2) Liquid fermentation culture

[0035] Add 10ml of sterile water to the slant of each test tube, scrape off the conidia with an inoculation loop, shake up to form a spore suspension, and insert 300ml of nutrient solution (soybean powder 10.0g, sucrose 20.0g) with a volume ratio of 0.2%. , Dipotassium Hydrogen Phosphate 2g, Ferrous Sulfate 0.02g, Calcium Carbonate 0.5g, Magnesium Sulfate 0.05g, Tap Water 1000ml, pH Natural.) in a 500ml Erlenmeyer flask, at a constant temperature of 150-200 rpm at 28°C for 7 ~8 days, smear, microscopic examination, produce a large number of spores, count ...

Embodiment 2

[0040] ——Preparation method of liquid fermentation preparation 2

[0041] (1) Strain preparation

[0042] With embodiment 1.

[0043] (2) Liquid fermentation culture

[0044] Divided into shake flask fermentation culture and fermenter tank fermentation culture. Shake flask fermentation culture is the same as the liquid fermentation culture in the above example except that the culture and fermentation time is shortened to 4 to 5 days. It is observed that the mycelium is abundant and a large number of new spores are formed on the mycelia. Up to more than 6 billion, obtain the mother species of fermenter. Take this again as the female species and insert the nutrient solution (10.0g of soybean powder, 20.0g of sucrose, 2g of dipotassium hydrogen phosphate, 0.02g of ferrous sulfate, 0.5g of calcium carbonate, 0.05g of magnesium sulfate, Tap water 1000ml, pH natural.) in a fermenter at a constant temperature of 28°C at a constant temperature of 150-200 rpm for 6-7 days, count th...

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Abstract

The invention discloses a biological agent for preventing and treating tobacco red spot disease. The production strain of the biological preparation is classified as Streptomyces globisporus AM6, and the preservation number is CGMCC No.1701. The liquid and solid culture medium formula of described bacterial strain is as follows: 1. test tube slant culture medium formula is: fresh potato 200g, glucose 20g, agar 17-20g, distilled water 1000ml, pH is natural; 2. liquid fermentation medium formula is: soybean flour 10.0g , 20.0g sucrose, 2g dipotassium hydrogen phosphate, 0.02g ferrous sulfate, 0.5g calcium carbonate, 0.05g magnesium sulfate, 1000ml tap water, natural pH. The invention is prepared as a biological preparation through the test tube culture of bacterial strains and shaker fermentation culture. The test results show that the biological agent provided by the invention has a good control effect on tobacco red spot disease.

Description

technical field [0001] The present invention relates to biopesticide, more specifically, the present invention relates to a kind of bacterial agent for preventing and treating tobacco red spot disease and its preparation method. Background technique [0002] Tobacco is an important foliar economic crop in my country, and it is one of the crops with the most serious disease damage. From sowing to harvesting, the whole process can be infected by pathogens. Tobacco Brown Spot Disease (Tobacco Brown Spot Disease) is an important leaf disease in the late mature stage of tobacco leaves. It commonly occurs in various smoking areas and is the main disease of tobacco in my country. In recent years, red spot disease has also developed in Hunan, Anhui, Guizhou, Yunnan, Shaanxi, Liaoning, Zhejiang, Guangdong, Fujian, Heilongjiang, Jilin and other provinces in China, becoming the most harmful leaf spot disease. 20% to 30%, the severe incidence rate reaches 90%, and the output value is r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00C12N1/20C12R1/465
Inventor 方敦煌邓云龙邓建华宋春满
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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