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Phosphorus-solubilizing bacteria, fungicide and application thereof

A technology of phosphorus-dissolving bacteria and bacterial agents, applied in the field of microorganisms, to achieve broad application prospects and market value, promote absorption and utilization, and strong phosphorus-dissolving ability

Active Publication Date: 2022-07-29
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the isolation of phosphate-solubilizing bacteria has received more and more attention, but these studies are mainly concentrated on dry land. Long-term flooding leads to a completely different ecological environment in paddy fields and dry land, and the development and utilization of specific phosphate-soluble bacteria resources are urgently needed.

Method used

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  • Phosphorus-solubilizing bacteria, fungicide and application thereof
  • Phosphorus-solubilizing bacteria, fungicide and application thereof
  • Phosphorus-solubilizing bacteria, fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening and identification of Streptomyces phosphatidylase ISA-C7 strain

[0026] 1. Strain screening

[0027] (1) Domestication and culture of phosphorus-dissolving bacteria: Take soil samples of 0-5 cm on the surface of subtropical paddy fields in Changsha City, Hunan Province, place them in sterile bags for low-temperature storage, and bring them back to the laboratory for storage at 4°C. The topsoil of the paddy field was made into a soil suspension, and the concentration was diluted to 10 -6 Add 0.5mL of insoluble calcium phosphate culture medium (NBRIY medium) to a sterile 96 deep-well plate, add 0.1g / L bromocresol violet as an acid indicator to the culture medium, and inoculate 10uL with a dilution concentration of 10 -6 times the soil suspension and placed in a constant temperature incubator (28°C) for 7-10 days. Then carry out the serial dilution again, take the prepared 10 -4 times and 10 -5 200uL of 200uL diluted bacterial solution was coated...

Embodiment 2

[0036] Example 2: Phosphorus-dissolving activity assay of Streptomyces phosphate-solubilizing bacteria ISA-C7

[0037] Phosphorus-dissolving ability of phosphate-dissolving bacteria in calcium phosphate solid medium is based on the phosphorus-dissolving index of phosphorus-dissolving bacteria on calcium phosphate solid petri dishes: the three-point inoculation method inoculates the screened phosphate-solubilizing bacteria into calcium phosphate solid medium, and places them at a constant temperature The incubator (28°C) was cultured for 5 days, and the growth status of phosphorus-dissolving bacteria on the petri dish was recorded by taking pictures with a camera. figure 1 This is the topography of the phosphate-dissolving circle after 5 days of culturing in calcium phosphate solid medium for Streptomyces phosphate solubilization ISA-C7. However, due to the lack of water in the calcium phosphate solid medium, it is not conducive to the reproduction of Streptomyces phosphate sol...

Embodiment 4

[0041] Example 4 Effect of Phospho-solubilizing bacteria Streptomyces ISA-C7 on the transformation of phosphorus in paddy soil

[0042] Soil sample collection and pretreatment: Soil samples were collected from the NPK (NPK) and NPK (NK) treatments in the long-term positioning rice experimental field in Jinjing, Hunan Province. The experimental field was in the drying stage. Three plots with different treatments were selected, and three topsoil cores (0-20 cm) were randomly taken from each plot with a soil drill, with a total of 9 soil cores, mixed uniformly, and used as a sample. The collected soil samples were manually removed from residual roots, and ddH was added. 2 O submerge, mix well, pass through a 2mm sieve, and let the soil settle at room temperature (about 2 to 3 days). Pour off the supernatant, divide each sample into two evenly, one is pre-incubated directly in a jar at 25°C, and the other is put into the same jar and autoclaved at 121°C for 30 min. Measure the r...

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Abstract

The invention relates to the technical field of microorganisms, and particularly discloses phosphate-solubilizing bacteria, a microbial agent and application of the phosphate-solubilizing bacteria and the microbial agent. The strain name of the phosphate-solubilizing bacteria is streptomyces ISA-C7, the classification name of the phosphate-solubilizing bacteria is streptomyces globisporus subsp. Globisporus, the phosphate-solubilizing bacteria are preserved in the China General Microbiological Culture Collection Center, the preservation number is CGMCC NO.23445, the preservation date is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the postcode is 100101. The Streptomyces sp. ISA-C7 has strong phosphate solubilizing ability in both a low-phosphorus environment and a high-phosphorus environment. Streptomyces ISA-C7 is developed into a microbial inoculum, is applied to plant fertilizers, and has important significance for effectively improving the content of rapidly available phosphorus in soil and the utilization rate of a phosphate fertilizer.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a phosphorus-dissolving bacteria, a bacterial agent and applications thereof. Background technique [0002] Phosphorus is a macronutrient essential for plant growth, participating in the entire life cycle of plants and playing a key role in several major metabolisms including photosynthesis, energy transfer, signal transduction and macromolecular biosynthesis. However, due to the strong adsorption of phosphorus by mineral particles, soil phosphorus availability is poor, resulting in low phosphorus fertilizer utilization. Since 1980, the accumulation of phosphorus in farmland soil in my country has exceeded 85 million tons of P. 2 O 5 , but the proportion of available phosphorus that can be used by crops is less than 1%. Due to the lack of effective activation measures, the phosphorus accumulated in the soil cannot be absorbed and utilized by plants, which not only cause...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/80C05F11/08C09K17/14C12R1/465
CPCC12N1/20C05F11/08C05G3/80C09K17/14C09K2101/00Y02W30/40
Inventor 葛体达魏亮肖谋良魏晓梦祝贞科陈剑平
Owner NINGBO UNIV
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