170results about How to "Increase available phosphorus content" patented technology

The invention discloses bacillus cereus with heavy metal passivating and plant growth promoting functions and application of the bacillus cereus, and belongs to the technical field of microbial strains. The name of the bacillus cereus is bacillus cereus B19, and is preserved in the China General Microbiological Culture Collection Management Committee Centre of Institute of Microbiology, Chinese Academy of Sciences, 3#, No.1 Yard, West Beicheng Road, Chaoyang District, Beijing, on April 27th, 2016; the preservation number is CGMCC NO. 12405. The bacillus cereus has the functions of passivating heavy metals and promoting plant growth, is suitable for decomposing difficultly soluble phosphorus, and is used for producing auxin, resisting salt, resisting the heavy metals, promoting plant seed germination, increasing effective phosphorus and microbial carbon and nitrogen of soil, and reducing available heavy metals in the soil.

The invention relates to the technical field of soil conditioners and particularly relates to a soil heavy metal biological fixing agent and an application method thereof. The formula of the soil heavy metal biological fixing agent is that every one kilogram of an organic fertilizer is added with 2-10 g (109 CFU/g) of B. natto solid leavening agent, 2-10 g (109 CFU/g) of B.mucilaginosus solid leavening agent, 2-6 g (109 CFU/g) of B.megaterium solid leavening agent and 4-10 g (1010 CFU/g) of Trichoderma koningii solid leavening agent, wherein the main ingredients of the biological organic fertilizer contain more than 40% of organic matter, more than 20 million/g of effective viable count, and more than 5% of total nutrient. The soil heavy metal biological fixing agent is low in cost, has few environmental risks, remarkably reduces the absorption of the heavy metals of the crops, improves the soil fertility, effectively prevents and cures the soil-borne diseases and increases the economic benefit of the crops.

The invention provides a sludge-derived soil remediation agent as well as a preparation method and application thereof in sludge digestion. According to the adopted technical scheme, pretreatment is carried out on urban sewage and sludge, and the sludge is treated by means of twice fermentation. Tests prove that after the sludge remediation agent is used for 20 days, the proportional effective removal rate of various pollutants in soil reaches 95% or more and is in line with national standards. Furthermore, a sulfuric acid substance can acidify the hardened saline-alkali soil and is also good organic fertilizer, thus beneficial to reduction of the use of chemical reagents and chemical fertilizer in agricultural production, and putting an end to pollution and secondary pollution from the source.

The invention relates to a biological control agent, a bioorganic fertilizer, and applications thereof. The biological control agent comprises Bacillus velezensis, Bacillus subtilis, Paenibacillus polymyxa, and Bacillus amyloliquefaciens. The provided biological control agent and bioorganic fertilizer can continuously inhibit fusarium oxysporum for a long term, and has a long-lasting control effect on plant blight, in particular, banana blight.

The invention relates to a composite microbial organic fertilizer for saline-alkali soil improvement. The composite microbial organic fertilizer for saline-alkali soil improvement comprises Bacillus subtilis, Bacillus licheniformis, Bacillus megaterium, silicate bacterium, mould fungus, fulvic acid, amino acids and bentonite. The preparation method comprises the following steps: screening, preparing a composite microbial inoculant, concentrating the fermentation liquid, drying, and carrying out high-temperature composting rotting fermentation. When the composite microbial organic fertilizer is used for improving the saline-alkali soil, the crop yield is increased by 19-40%, the soil pH is lowered by 0.2-0.8, the electric conductivity (EC) is lowered by 0.5-0.8, the available phosphorus content is increased by 2.0-4.4 mg/kg, the quick-acting potassium content is increased by 40-68 mg/kg, and the quantity and diversity of soil microbes are obviously increased.

The invention belongs to the technical field of plant rhizosphere growth-promoting bacteria and particularly discloses Klebsiella sp. P5 with a phosphorus dissolving function and application of the Klebsiella sp. P5. The Klebsiella sp. CCNWSX1902 P5 is collected in the China Center for Type Culture Collection (CCTCC) on April 22, 2019, with the collection number of CCTCC NO:M 2019280. The phosphorus dissolving bacterium P5 is separated from wheat rhizosphere soil and identified as the Klebsiella sp. CCNWSX1902 in the aspects of morphology, physiological-biochemical characteristics and genetics. The strain can effectively dissolve indissolvable phosphorus in plant rhizosphere soil, substantially increase the content of rapidly available phosphorus in the soil and increase the utilization rate of phosphorus in the soil by plants to promote plant growth, reduce the dosage of chemical fertilizer and increase the yield of crops, and has important significance in agricultural production.

The invention relates to the technical field of functional microorganism screening, and in particular provides an aspergillus niger strain having a phosphorus-solubilizing function; the aspergillus niger strain is preserved with preservation number of CCTCC NO: M2015004. Through aspergillus niger, insoluble phosphorus (Ca3(PO4)2) in an insoluble inorganic phosphorus liquid medium can be decomposed into soluble available phosphorus, so that the content of available phosphorus in the medium can reach up to 0.680mg/mL, and a phosphorus-solubilizing effect is significant. In rape field cultivation experiments, the aspergillus niger screened by the invention can effectively improve the content of available phosphorus in soil, so as to greatly increase the yield of planted crops and to achieve an unexpected technical effect. The aspergillus niger can be used for preparing phosphorus-solubilizing bacteria; and the aspergillus niger is widely applicable to agricultural production and is broad in prospect.

The invention provides a high salt-resistant phosphate solubilizing bacterial strain and application thereof, and relates to the technical field of agricultural planting and microbes. The high salt-resistant phosphate solubilizing bacterial strain has the code name of QDZN-13, is identified to be bacillus subtilis, can survive and colonize in a high-salinity environment, has the culture collectionnumber of CGMCC No.14805, and is collected in the China General Microbiological Culture Collection Center on October 12, 2017. The high salt-resistant phosphate solubilizing bacterial strain QDZN-13can be applied to high-salinity soil, and has relatively high phosphate solubilizing activity; the high salt-resistant phosphate solubilizing bacterial strain QDZN-13 can be mixed with bacillus licheniformis and bacillus megaterium to obtain a compound microbial inoculum which is applied to a microbial fertilizer; the high salt-resistant phosphate solubilizing bacterial strain QDZN-13 can be usedas a phosphate solubilizing microbial inoculum which is applied to the microbial fertilizer alone, and is applied to a high-salinity and high-alkality land for decomposing insoluble phosphorus in soilto promote conversion of the insoluble phosphorus into soluble phosphorus capable of being absorbed and utilized by plants and promoting absorption of the plants to other nutrients. The high salt-resistant phosphate solubilizing bacterial strain has the beneficial effects of improving soil, especially the physical and chemical properties of the saline-alkali land, increasing absorbable phosphorusin the soil and effectively promoting plant growth.

The invention discloses a saline-alkaline tolerance phosphorus solublizing bacteria strain and application thereof, and belongs to the technical field of microorganisms. The saline-alkaline tolerancephosphorus solublizing bacteria strain is named phosphorus solublizing bacteria strain RPB03, which is preserved in the China General Microbiological Culture Collection Center, with a preservation number of CGMCC No:15928, on June 11, 2018. The strain is high in adaptability to a high-salt and high-alkali environment, can increase the effective phosphorus content in calcium phosphate fermented liquid to 430 mg/L and grows well in a saline-alkaline soil environment, and indigenous microorganisms have little impact on the phosphorus solublizing effect of the saline-alkaline tolerance phosphorussolublizing bacteria strain; the effective utilization rate of phosphorus in saline-alkaline soil in a saline-alkaline farmland, a forest and the like can be effectively increased, and the plant biomass is increased. Due to the application of the strain, soil hardening caused by use of chemical fertilizer can be relieved; the utilization rate of soil phosphorus is increased; the environmental pollution is reduced.

The invention discloses pseudomonas aeruginosa DGNK-JL2. The pseudomonas aeruginosa DGNK-JL2 is collected by a Guangdong Microbial Culture Collection Center, which is located at fifth floor of building 59# of yard 100#, middle Xianlie road, Guangzhou, Guangdong 510075, on Aug 22, 2019 and has the collection number of GDMCC NO: 60748. A strain of phosphate solubilizing bacteria, i.e., the pseudomonas aeruginosa DGNK-JL2 is separated and screened out from traditional Chinese medicine decoction dreg compost and is subjected to identification and biological control action tests, so that a strain for preparing efficient biofertilizers is reserved, and the sustainable development of ecological agriculture is boosted; and by employing the pseudomonas aeruginosa DGNK-JL2 disclosed by the invention, the content of available phosphorus in soil can be increased by 8.6 times to the maximum, and fermentation broth of the strain DGNK-JL2 disclosed by the invention has a certain antagonistic action on pepper anthracnose and bitter melon blight.

The invention provides a microbial soil improving agent for restoring petroleum polluted soil. A product is prepared from a degradation bacterial agent, an additive and a biosurfactant, wherein the degradation bacterial agent is prepared from pseudomonas, bacillus, Acinetobacter, microzyme, phosphate-solubilizing bacteria, azotobacterial, nitrobacteria and photosynthetic bacteria; the additive isprepared from the following ingredients in parts by weight: 20 to 35 parts of wormcast, 80 to 120 parts of bentonite, 1 to 10 parts of chitosan, 10 to 20 parts of kaolin, 5 to 9 parts of borax, 25 to35 parts of crop stalks, 10 to 15 parts of rice hull powder, 10 to 15 parts of dry seaweed, 1 to 5 parts of organic acid, 5 to 10 parts of vermiculite, 10 to 20 parts of shell powder and 20 to 25 parts of compound amino acid. The microbial soil improving agent for restoring petroleum polluted soil has the advantages that the soil fertility is improved; the petroleum in the soil can be degraded; the goal of governing the petroleum polluted soil is achieved.

The invention relates to an acidic soil conditioner and a preparation method thereof. The method comprises the following steps: preparation of a phosphate-containing solid from glyphosate waste liquid; preparation of a soil conditioner; granulation; drying; etc. Compared with CK, the pH value, effective phosphorus content and rapidly-available potassium content in soil of a capsicum area where the acidic soil conditioner is applied are increased by 0.44 unit, 21.60% and 33.87%, respectively; as for disease resistance, the incidence of capsicum anthracnose and the incidence of capsicum phytophthora blight are reduced by 17.83% and 26.21% compared with a control group, respectively; moreover, the contents of cadmium, lead, total mercury and total arsenic in capsicum fruit are reduced by 24.21%, 23.37%, 31.58% and 17.39%, respectively. According to the invention, recycling sustainable utilization of high-pollution waste water in production of glyphosate is realized; the pH value of acidic soil is increased; and disease resistivity and harmful heavy metal ion absorbing capacity of plants are improved.

The invention relates to a method for preparing high-efficiency phosphorus fertilizer without acidification. In the method, the material is ground by a vibration milling wet method to prepare super fine activated phosphate rock powder which has high specific surface area, the particle size is 90 percent and can passes through a 400-mesh sieve, the super fine activated phosphate rock powder is mixed with biological organic fertilizer according to the weight ratio of between 1 to 4 and 1 to 1, and pseudomonas fluorescens is added for mixing stockpile manure and fermenting so as to prepare the non-acidification high-efficiency phosphorus fertilizer. The method has the advantages of preparing the high-efficiency phosphorus fertilizer by physical and biological methods, reducing the environmental pollution, solving the problems of environmental pollution, high cost and the like existing in the prior phosphorus fertilizer preparation method and lowering cost of producing the phosphorus fertilizer, along with simple technique; moreover, in long-term usage, the phosphorus fertilizer can prevent the sealing of soil, improve the soil and enhance the soil fertility.

The invention belongs to the technical field of plant growth promoting rhizobacteria and particularly relates to inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 and application thereof.The inorganic phosphorus solubilizing plant growth promoting rhizobacteria strain NG-33 is taxonomically named as Enterobacter cloacae NG-33 and preserved in the China Center for Type Culture Collection, and a preservation number of the plant growth promoting rhizobacteria strain NG-33 is CCTCC M 2016056.The plant growth promoting rhizobacteria strain NG-33 is obtained for the first time by screening and separating from soil of the Longgang National Natural Reserve in the Guangxi Zhuang Autonomous Region and is capable of realizing high-yield gluconic acid and acetic acid, dissolving hardly-soluble inorganic phosphorus to increase soluble phosphorus content in soil, relieving phosphorus deficiency of plants and improving soil conditions, and accordingly plant growth is promoted.

The present invention discloses a carbon-based composite biological fertilizer. The carbon-based composite biological fertilizer is prepared from the following raw materials in parts by weight: 20-60parts of biochar, 10-30 parts of plant ash, 8-25 parts of medical stone powder, 3-15 parts of zeolite powder, 2-8 parts of potassium feldspar powder, 1-6 parts of ground phosphate rock, 15-45 parts ofhumic acid, 1-10 parts of potassium fulvate and 1-15 parts of photosynthetic nitrogen fixation composite bacteria. The carbon-based composite biological fertilizer gives full play to the respective advantages of photosynthetic nitrogen fixation composite bacteria, biochar, plant ash, medical stone powder, zeolite powder, potassium feldspar powder, ground phosphate rock, humic acid and potassium fulvic acid, not only realizes effective improvement and remediation of soil and improves the crop yield, but also is beneficial to promoting the development of green low-carbon agriculture. The invention also provides a preparation method of the carbon-based composite biological fertilizer, which is simple and convenient to operate and convenient for batch production. The invention also provides application of the carbon-based composite biological fertilizer, and the carbon-based composite biological fertilizer is compounded with one or more of decomposed manure, an organic fertilizer, slag, biomass pyrolysis residues or crop wastes according to different types of soil, so that the fertilizer efficiency is exerted.

The invention discloses an efficient phosphate solubilizing bacillus megaterium in a tree rhizosphere. The preservation number is CCTCC NO. M 2017454, and the efficient phosphate solubilizing bacillusmegaterium is named as Bacillus megaterium L205. The test shows that the phosphate solubilizing amount of bacillus megaterium reaches 203.04mg/L, and the liquid bacterium can effectively solubilize insoluble inorganic phosphorus, increase the content of available phosphorus in red-yellow soil, and promote the growth of tree seedlings. The efficient phosphate solubilizing bacillus megaterium provided by the invention enriches the sink population of bacillus megaterium at the culture collection center and provides convenience for better exploitation of microbial population resources of bacillusmegaterium. Meanwhile, the bacillus megaterium strain L205 can secrete organic acids, solubilize inorganic phosphorus and improve the phosphorus absorption and utilization of plants, is especially suitable for the production of tree biological fertilizers and tree growth promoting bacteria in red-yellow soil areas in South China, and can be used for further developing compound microbial fertilizers and bacteria.

The invention relates to a method for constructing a soil ploughing layer of a south-Xinjiang oasis drop-irrigation continuous-cropping middle-low-yield cotton field. The method includes the steps that before sowing in the cotton field, straw of last crops is smashed and returned to the field; farmyard manure and chemical fertilizer are applied; deep ploughing, cotton field flattening and super-deep scarification are conducted; irrigation is conducted before sowing; sowing is conducted; furrows are dug in the cotton field, and anti-salt plants are planted; a windbreak tree belt is built on the periphery of the cotton field; a water-irrigation and drainage trench system is built; field management is conducted. After the method is adopted, the surveying result shows that the soil water content in the seedling stage is increased by 0.5%, rapidly-available potassium of the ploughing layer is increased by 35.2%, the temperature of soil 5 cm lower than films is increased by 0.6 DEG C, the thickness of the ploughing layer in the seedling period is increased by 7.1 cm, the salt content of soil is reduced by 2.1 g/kg, the soil porosity is increased by 8.5%, the soil penetration resistance (PSI) is reduced by 130, the soil bulk density is reduced by 0.11 g/cm<3>, the pH value of soil is reduced by 0.4, the residual film content of soil of the ploughing layer is reduced by 11.3 kg per mu, premature senescence of the cotton field is avoided, the yield of seed cotton is increased by 57.5 kg per mu, and therefore the method has the soil-improving and yield-increasing effects and can meet production requirements.

The invention relates to a lignin organic slow-release liquid fertilizer for wheat and a preparation method thereof, belonging to the technical field of organic slow-release fertilizers. The lignin organic slow-release liquid fertilizer for wheat is prepared from the following raw materials in parts by weight: 20-35 parts of lignin, 10-20 parts of a high molecular polymer, 2-8 parts of cellulose mud cakes, 3-7 parts of organic abamectin, 10-15 parts of acidic hemicellulose liquid, 10-15 parts of alkaline hemicelluloses liquid, 8-13 parts of a nitrogen fertilizer, 10-15 parts of a phosphorus fertilizer, 12-16 parts of a potassium fertilizer, 5-10 parts of nitric acid, 10-20 parts of plant cytokinin, 16-20 parts of zeolite powder, and 5-10 parts of activated carbon. The raw material components in the lignin organic slow-release liquid fertilizer for wheat coordinate with one another to achieve a synergistic action, the nutrient proportions of the raw materials consider the total nutrient requirement in the whole growth period of the wheat to ensure that the nutrient elements required by the growth and development of the wheat can be comprehensively supplied, so that the lignin organic slow-release liquid fertilizer is complete in nutrition, more in function, high in comprehensive fertility and great in potentiality and is special for the wheat.

The invention provides a microbial organic fertilizer for improving saline-alkali soil. The fertilizer comprises the following raw materials: fulvic acid, peat, salicylic acid, municipal sludge, sesame paste, mannitol, sulfated polysaccharide, cymene, rare earth isoprene rubber powder, alfalfa plant, pumice, psyllium husk powder, salt tolerant Rhodopseudomonas capsulata, and Halomonas halophiles. The invention also provides a preparation method of the fertilizer. The fertilizer prepared by the method provided by the invention has an effective living bacteria number of 0.74-0.77 billion cfu/g, a total nitrogen content of 3.8-4.1wt%, a total phosphorus content of 2.9-3.2wt%, a total potassium content of 3.2-3.6wt%, an organic matter content (on a dry basis) of 43.3-44.7wt%, a water content of 22.1-22.6wt%, and a pH of 5.52-5.58. The fertilizer provided by the invention has a saline-alkali soil improving effect, and can reduce the pH value of soil, the soil alkalization degree and soil salinity.

The invention discloses a compound soil repair agent, a preparation method and a repairing method thereof. The repair agent is composed of a hydroxyapatite-calcium silicate composite material and biochar, the mass ratio of the hydroxyapatite-calcium silicate composite material and the biochar is 4 to 6-9 to 1, and the biochar is poplar base biochar. The preparation method comprises the steps thatdispersion liquid of calcium chloride and dispersion liquid of sodium silicate are correspondingly prepared firstly, then a calcium chloride solution is dropwise added into a sodium silicate solutionunder ultrasonic to react, washing by deionized water and filtering are conducted after reaction is completed, calcium silicate hydrate precipitation is obtained and added into a phosphate solution toreact, the hydroxyapatite-calcium silicate composite material is obtained after filtering, and then the biochar is mixed with the composite material according to the mass ratio to obtain the soil repair agent. According to the compound repair agent, when the ratio of the hydroxyapatite-calcium silicate composite material and the biochar is 4 to 6, mutual promotion between the materials is achieved, the synergistic effect is generated, the long-term and high efficient repair effect can be achieved for heavy composite metal contaminated soil, the cost is low, the using amount is small, and environmental protection is achieved.