18results about How to "Increase available phosphorus content" patented technology

The present invention relates to the field of microbial technology, and in particular to a strain of Pseudomonas fluorescens LSOJM27 and its application and bacterial agent. The Pseudomonas fluorescens LSOJM27 was deposited in the China Center for Type Culture Collection on December 29, 2022, with a deposit number of M 20222101. The strain provided by the present invention has a strong phosphate solubilizing ability and is suitable for activating phosphorus in cultivated soil. The phosphate solubilizing bacteria can significantly increase the content of available phosphorus in cultivated soil and reduce the application of phosphate fertilizers.

The invention provides a strain of Ralstonia sp. Regulated by quorum sensing and application thereof, the strain is Ralstonia sp., is named as YTR-R242, has been preserved in China General Microbiological Culture Collection Center (CGMCC) on October 21, 2024, and has a preservation number of CGMCC32269. The strain can generate N-acyl-homoserine lactone, the structure of the strain is C4-HSL, a phosphate-solubilizing ring proves that the Ralstonia sp. Strain YTR-R242 has a phosphate-solubilizing characteristic, and when the Ralstonia sp. Strain YTR-R242 is prepared into a bacterial agent for use, the content of available phosphorus in red soil is remarkably increased, and the growth condition of corn is improved.

The invention belongs to the technical field of soil conservation, and particularly relates to a method for promoting plant growth and regulating soil nutrients. The invention discloses a method for regulating and controlling corn and tobacco growth and rhizosphere soil nutrients through combination of AMF and a planting mode. The method comprises the following steps: preparing an AMF-containing microbial inoculum by using M. mosseae (F. mosseae); cultivating flue-cured tobacco seedlings and corn seedlings; flue-cured tobacco seedlings and corn seedlings are jointly planted in an intercropping mode by utilizing an AMF-containing fungicide. The method can relieve continuous cropping obstacles of flue-cured tobaccos and promote growth of the flue-cured tobaccos and corn, and has a certain effect on soil conservation.

The invention discloses preparation and application of a biological carbon type phosphate fertilizer synergist suitable for flue-cured tobacco, and relates to the technical field of phosphate fertilizer synergists. Comprising the following steps: step 1, preparing raw materials; 2, raw material treatment; and step 3, mixing and reacting: by taking the charcoal and the humic acid as main raw materials, the content of available phosphorus in soil can be increased, absorption and utilization of tobacco to calcium superphosphate and diammonium phosphate are increased, growth and development of the tobacco are promoted, the fresh weight of the tobacco leaves is increased, and the phosphorus accumulation amount of the tobacco is increased. According to the preparation and application of the biological carbon type phosphate fertilizer synergist suitable for flue-cured tobacco, the fixing agent, the water-retaining agent and the microbial agent are added into the biological carbon source to form the phosphate fertilizer synergist, the water-retaining agent can prevent water loss in soil, and the fixing agent can prevent phosphorus loss in the soil; and a mixture of compound sodium nitrophenolate and high-purity alpha-pimacol is added, so that the permeation, adhesion and spreading force of the fertilizer are increased, the limitation of plants is broken, and the capability of the fertilizer entering the plants is enhanced.

The invention relates to the technical field of fertilizer preparation, and particularly discloses a modified flotation phosphate tailing-based composite calcium magnesium phosphate fertilizer and a preparation method thereof, and the composite calcium magnesium phosphate fertilizer is prepared from modified flotation phosphate tailings, dolomite, limestone, an activator, a binder, a magnesium supplement conditioner and water. The flotation phosphate tailings are deeply modified by adopting a composite pretreatment liquid synergistic activation-gradient composite modification process, so that the effectiveness of nutrients such as phosphorus, calcium and magnesium in the flotation phosphate tailings and the environmental safety are remarkably improved; and through the raw material compounding design of calcium and magnesium source collaboration, gradient magnesium supplementation and conditioning synergism, quick-acting and long-acting collaborative supply of nutrients and a soil improvement function are realized. The flotation phosphate tailings are used as main raw materials, the technological process is simple, energy consumption is low, the prepared composite calcium magnesium phosphate fertilizer is balanced in nutrient, lasting in fertilizer efficiency and excellent in physical performance, and unification of solid waste recycling, product high value and production greenization is achieved.

The invention discloses a rhizosphere growth-promoting bacterium of sheep grass and its application, belonging to the field of microbial technology. The strain is classified and named as Phyllobacterium myrsinacearum, strain number B68, and deposit number CGMCC No.31906. The strain of the present invention is separated from the rhizosphere soil of sheep grass in an overgrazing sample, and is obtained by artificial separation and purification. The strain has high yield of 3-indoleacetic acid, has strong solubility for insoluble phosphates, and has good growth-promoting properties such as nitrogen fixation, ACC deaminase production, and IAA production. The prepared strain fermentation liquid is inoculated into sheep grass seedlings, and the strain can be colonized in the rhizosphere of sheep grass, regulate the bacterial community structure of rhizosphere soil of sheep grass; significantly promote the growth of sheep grass, enhance photosynthesis, and increase chlorophyll content; significantly increase the hormone and nutrient content in plant leaves and roots; significantly increase the content of available phosphorus, total carbon and organic matter in rhizosphere soil. Therefore, the myrsinacearum leaf bacillus of the present invention can be used for the development and related research of subsequent microbial fertilizers for grazing grasslands.

The present invention discloses an endophytic fungus G22 from the root of Fujian cypress and its application. The endophytic fungus G22 is classified and named as Trichoderma harzianum ( TrichodermaHarzianum ) G22, which was deposited in the General Microbiology Center of the China Microbiological Culture Collection Committee on October 13, 2023, with the deposit number CGMCC No.40845, and the deposit address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Through potted phosphorus solubilization test and growth promotion analysis, it was found that the endophytic fungus G22 can convert the insoluble phosphorus in the soil that is difficult for plants to absorb and utilize into soluble effective phosphorus that can be directly absorbed and utilized, which can better promote plant growth and increase the plant seedling height, ground diameter, total root length, root surface area and root volume.

A strain of Talaromyces fungus W10 and its application belong to the field of agricultural microbial technology. In order to screen fungi with efficient phosphate-dissolving activity and salt tolerance, and then provide alternative strains for the application of phosphate-dissolving fungi in agriculture, the present invention screened a strain of efficient phosphate-dissolving fungus W10 from the rhizosphere soil of phosphate plants, and identified the strain W10 as a Talaromyces fungus through morphological identification and molecular biological identification. Through the study of phosphate-dissolving effect and growth-promoting effect, it was found that strain W10 has a good phosphate-dissolving effect on a variety of insoluble phosphorus, and can tolerate a salt concentration of up to 4%. It still has good phosphate-dissolving activity under a salt concentration of 4%, and has the potential to be developed as a saline-alkali land microbial fertilizer; in addition, strain W10 can promote the growth of corn seedlings, increase the effective phosphorus content and urease and sucrase activities in the rhizosphere soil of corn seedlings. It can be seen that strain W10 has broad application prospects in agricultural production.

The present invention discloses a novel Paenibacillus sp. SN‑8‑1 and its application, and relates to the field of microbial technology. The novel Paenibacillus sp. SN‑8‑1 disclosed in the present invention is classified and named Paenibacillus sp. SN‑8‑1, which has been deposited in the China Microbial Culture Collection Center, with a deposit number of CGMCCNO.1.60135, a deposit date of January 16, 2024, and a deposit address of: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The novel Paenibacillus sp. SN‑8‑1 of the present application effectively promotes the increase of soybean plant height, the increase of fresh weight of the above-ground part of soybean, and the increase of fresh weight of the underground part of soybean; secretes organic acids and phosphatases; and can effectively dissolve insoluble inorganic phosphorus. It is used to solve the problem of low phosphorus utilization efficiency in agricultural and forestry land, and has good application and promotion value.

The invention discloses a microbial agent for increasing available phosphorus in alkaline soil and application of the microbial agent, and belongs to the technical field of microbial agents and soil modification. The microbial agent for increasing the available phosphorus in the alkaline soil is prepared by taking the microbial compound bacterium powder, the adsorbent and the acid conditioner as raw materials, and the microbial compound bacterium powder can convert insoluble phosphorus in the soil into the available phosphorus which can be absorbed by crops, so that the content of the available phosphorus in the soil is increased; the improvement of crop yield and quality is promoted; on one hand, the adsorbent can adsorb microorganisms to avoid non-uniform distribution of available phosphorus capable of being utilized by plants in soil due to non-uniform application of a fungicide and the like, and on the other hand, the adsorbent can provide a good condition for propagation of the microorganisms to give full play to the conversion effect of the microorganisms; and the acid conditioner and the microbial compound bacterium powder cooperate to condition the soil, so that the alkaline soil has a more lasting conditioning effect, and the alkaline soil is thoroughly improved for a long time.

This application relates to the field of microbial technology, and in particular to a method and application for improving soil bioavailable phosphorus using protozoa. This application aims to utilize a soil-environment-friendly protozoan method for improving soil bioavailable phosphorus. The method of this application has advantages such as simple and convenient operation, low cost, environmental friendliness, a rich selection of microbial strains, in-situ application, and avoidance of nutrient loss, low utilization rate, pathogen contamination, and heavy metal contamination caused by excessive fertilization. Simultaneously, it significantly increases the organic phosphorus content of the soil.

The invention discloses a microbial preparation for passivating soil heavy metal cadmium and improving crop yield and quality and application of the microbial preparation, and belongs to the technical field of microbial preparations. The rhizopus arrhizus WG01 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on September 9, 2024, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41501. The microbial solid preparation is prepared by fermenting two strains of rhizopus arrhizus WG01 and burkholderia by taking wheat bran and charcoal as carrier auxiliary materials, and then compounding with calcium superphosphate in proportion. When the microbial preparation is applied to soil, heavy metal cadmium in the soil can be passivated through the effects of adsorption, precipitation and the like, meanwhile, the content of organic matter and rapidly available phosphorus in the soil is increased through the addition of wheat bran, biochar, inorganic phosphorus and the like and the phosphorus dissolving effect of microorganisms, and yield increase and quality improvement of crops are achieved. The microbial solid preparation is convenient to use, green and pollution-free, and has the advantages of passivating soil heavy metals, improving soil fertility, increasing crop yield and the like.

The present invention provides a strain of Bacillus nicotinoic acid BD-5-3 and an application thereof, belonging to the field of biotechnology, and the preservation number of Bacillus nicotinoic acid is: CCTCC NO: M 20232178. The Bacillus nicotinoic acid BD-5-3 provided by the present invention can be used for phosphate solubilization.

The invention provides a phosphate solubilizing agent applied to soil for replacing a phosphate fertilizer as well as a preparation method and application of the phosphate solubilizing agent. The phosphate solubilizing agent is prepared from the following raw materials in parts by mass: 1-15 parts of a composite initiator, 1-10 parts of organic acid containing carbon-carbon double bonds, 1-10 parts of unsaturated organic salt containing sulfonic acid, 0.5-5 parts of organic ester containing carbon-carbon double bonds and 1-80 parts of solvent water. The preparation method of the phosphate solubilizing agent is simple, efficient and low in cost, injection of extra inert protective gas is not needed in the industrial production process, potential risks in the industrial production process are reduced, and the cost is further reduced. The phosphate solubilizing agent disclosed by the invention is green, safe and environment-friendly, and the carboxyl group, the sulfonic group and the like of the phosphate solubilizing agent can degrade indissolvable phosphorus salt existing in soil and are not easy to gather, so that the content of available phosphorus in the soil is increased to increase the crop yield, and a new direction is provided for the development of phosphorus additives and alternative chemical fertilizers in the future agricultural field.

This invention provides a biological method for activating organic phosphorus in gleyed paddy soil, belonging to the field of soil improvement technology. The biological method involves introducing green algae into the paddy field after the rice seedlings have turned green, artificially cultivating a periclump biological community. This community utilizes the photosynthetic oxygen release from the periclump to increase the soil's redox potential and soil microbial activity, accelerating organic matter mineralization. Simultaneously, the method introduces phosphate-solubilizing bacteria into the paddy field after the rice enters the tillering stage, increasing the abundance and activity of phosphate-solubilizing bacteria in the soil. These bacteria then produce phosphatases, further accelerating organic phosphorus mineralization. The biological method provided by this invention effectively increases the abundance and activity of phosphate-solubilizing bacteria in gleyed paddy soil, thereby increasing the available phosphorus content in the soil.

The application belongs to the technical field of fertilizers, and provides a preparation method of modified fulvic acid and application of the modified fulvic acid in fertilizers.The preparation method of the modified fulvic acid comprises the following steps: step 1, dissolving fulvic acid in deionized water, ultrasonic activation, after reaction, drying to obtain activated fulvic acid; step 2, dissolving the activated fulvic acid in a sodium hydroxide solution, heating to 40-45 DEG C for 10-15 min, after cooling, adjusting pH, adding a polymer monomer and an initiator, heating to 40-45 DEG C for 3-3.5 h, and drying to obtain modified fulvic acid. The application introduces new functional groups, such as amide groups, into fulvic acid molecules through chemical reactions, so as to enhance the chelating capacity or adsorption performance of the fulvic acid on specific nutrient elements, thereby effectively preventing the fixation of the elements in soil, and improving the absorption efficiency of the elements by plants.

The invention relates to the technical field of crop planting, in particular to a method for planting cherry tomatoes based on straw in-situ field returning and application. The method comprises the following steps: crushing a crop of cherry tomato plants in a greenhouse, spreading the crushed cherry tomato plants on the ground, spreading a straw-decomposing inoculant, deeply plowing, irrigating, covering a film, closing the greenhouse, spreading wormcast after closing the greenhouse, pre-dripping 15-20 days before field planting, irrigating field planting water after field planting of cherry tomato seedlings on ridges, and spreading rice husks between rows after one month of field planting. The in-situ straw returning can significantly increase the leaf length, the leaf width, the leaf area and the chlorophyll content of cherry tomatoes. The in-situ straw returning can significantly reduce the fruit setting node position of the first ear, and significantly increase the fruit setting number of the single ear and the weight of the single fruit. The number of root systems can be obviously increased by returning the straw to the field in situ; the soil quick-acting phosphorus content is obviously increased. In conclusion, the straw in-situ returning-to-field technology is widely popularized and applied in the tomato production of the solar greenhouse.

The invention discloses a phosphorus-solubilizing fungus soil remediation agent as well as a preparation method and application thereof, and belongs to the technical field of soil remediation. The phosphorus-solubilizing fungus soil remediation agent comprises micro-nano bone black and a penicillium deserticola solid fungicide. The preparation method comprises the following steps: pyrolyzing pig bones, and grinding to obtain the micro-nano bone black; the method comprises the following steps: coating a solid culture medium with penicillium deserticola for culturing to obtain a solid plate, washing the solid plate after spores are mature to obtain a spore liquid, mixing a solid carrier with sterile water, then adding a solid fungicide protective agent, and finally adding the spore liquid to obtain the penicillium deserticola solid fungicide. The phosphorus-solubilizing fungus soil remediation agent provided by the invention not only can effectively promote dissolution of insoluble phosphate, but also can fix heavy metal cadmium (Cd) in soil, has a very good application value for farmland Cd pollution remediation and environmental protection, is green and environment-friendly, can comprehensively improve soil fertility, and creates a good nutritional condition for crop growth.