207 results about "Penicillium oxalicum" patented technology

The invention provides a phosphorus dissolvable penicillium oxalicum P8, which has the function of dissolving various kinds of indissolvable phosphorus in soil, is suitable for red soil in the South,black soil, drab soil and moisture soil in the North and other types of soil, and has various functions of improving the crop yield, activating the indissolvable phosphorus in the soil, preventing thesoil chemical fixation of phosphorus fertilizers and improving the utilization efficiency of the phosphorus fertilizers.

The invention relates to phosphorus-solubilizing fungi and application thereof to preparation of a biological fungus fertilizer, belonging to the technical field of microbial fertilizers. The microorganism in the invention is a penicillium oxalicum Mo-Po strain which is preserved at the China General Microbiological Culture Collection Center on September 25th, 2010 with a collection number of CGMCC No. 4209. An ITS4 gene sequence of the Mo-Po strain is a main characteristic basis for identifying the strain, wherein the sequence number of the sequence is HQ703581 in a Gene Bank database. Tobacco waste can be thoroughly decomposed and converted into the biological fungus fertilizer by the microorganism with a composting fermentation method. The prepared microorganism has a good effect of promoting plant growth and can be used for substituting a chemical phosphorus fertilizer. The prepared biological fungus fertilizer has the characteristics of low cost, high fertilizer efficiency, no residue and safety to people and livestock and is a high-efficiency biological fungus fertilizer in organic agricultural planting.

The invention discloses an oxalic acid penicillium strain NJDL-03 and application thereof. The strain NJDL-03 belongs to oxalic acid penicillium and is preserved in General Microorganism Center of China Committee for Culture Collection of Microorganisms on eighth, July, 2015, and the strain preservation number is CGMCC No. 11061. By means of the oxalic acid penicillium strain NJDL-03, solubility of hydroxyapatite can be remarkably improved, a large number of phosphate ions (PO<4><3->) are released out, hydroxyapatite lead [pb<10>(PO<4>)<6>(OH)<2>] which is quite prone to precipitating and fairly stable is generated through Pb<2+> in a precipitation environment, effectiveness of lead in the environment is lowered, and thereby the environment polluted by lead is restored. Oxalic acid penicillium fertilizer prepared through solid fermentation of the strain NJDL-03 is remarkable in application effects of improving solubility of hydroxylapatite, restoring lead pollution of soil, raising the utilization rate of calcareous soil phosphorus and promoting crop growth.

The invention discloses a strain penicillium oxalicum with high-efficiency phosphate solubilizing characteristic, which is classified and named as Penicillium oxalicum. The penicillium oxalicum has been preserved in the China General Microbiological Culture Collection Center (CGMCC) with the preservation number: CGMCC NO.7699. The invention further discloses tolerance of high-efficiency phosphate-solubilizing penicillium oxalicum to heavy metals. The strain has strong dissolving effect on insoluble phosphate tricalcium phosphate, magnesium phosphate and aluminum phosphate under the condition of shake-flask liquid culture condition, the dissolving effect on the tricalcium phosphate and magnesium phosphate can reach 100%, and the dissolving effect on the aluminum phosphate can reach 95.5%. The penicillium oxalicum is respectively inoculated into liquid culture liquor containing heavy metals such as Pb<2+>, Zn<2+>, Cr<2+>, Mn<2+>, Cu<2+>, As<5+> and Cd<2+>, has strong resistance to the heavy metals, especially, the concentration of tolerance to the Pb<2+>, Zn<2+>, Cr<2+> and Mn<2+> can respectively reach 2000mg / L. Therefore, the penicillium oxalicum can provide excellent strain resources for developing heavy metal-resistant high-efficiency phosphate-solubilizing bacteria agents.

The invention discloses a bioorganic fertilizer for adsorbing heavy metals and improving greenhouse soil. Raw materials of the bioorganic fertilizer comprise, by weight, 83-88 parts of a decomposed organic fertilizer, 8-12 parts of biochar, 1-2 parts of attapulgite, 2-3 parts of functional bacteria, and 0.5-1 part of methyl salicylate, wherein raw materials of the decomposed organic fertilizer comprise, by weight, 45-60 parts of straws, 30-45 parts of traditional Chinese medicinal residues, 1-5 parts of a urea fertilizer with the nitrogen content of 46wt%, 1-2 parts of a straw fermentation inoculant, 1-2 parts of penicillium oxalicum and 0.5-1 part of brown sugar. The content of organic matters in the bioorganic fertilizer is not lower than 30%, the quantity of effective live bacteria in the bioorganic fertilizer is not lower than 0.02 billions / g, and the bioorganic fertilizer can effectively enrich heavy metals in soil and reduces the content of the heavy metals; and functional bacteria and methyl salicylate can dually defense internally and externally, can effectively inhibit breeding and invasion of root-knot nematodes, also can improve the structure of soil, increases the permeability, improves the pH value, and reaches yield increase, quality improvement and environmental protection.

The invention discloses a penicillium sp. mutant strain and application of the penicillium sp. mutant strain to cellulase preparation. The invention provides a strain of penicillium oxalicum EU2106 with the preservation number being CGMCC (China General Microbiological Center Culture Collection Center) No.6471. The invention also provides a method for producing cellulase or a cellulase preparation. The method comprises the following steps that the penicillium sp. mutant strain EU2106 is cultured for a certain time on a basic or optimized liquid culture medium, culture substances are collected, thalli are removed through centrifugation, and then, obtained supernate can be used as the cellulase preparation. Experiments prove that the penicillium oxalicum EU2106 obtained through the mutation can be used for producing the cellulase preparation, the preparation can be used for effectively hydrolyzing sugarcane slag paper pulp, and in addition, glucose is major ingredients in the final hydrolysis products. The cellulase preparation can be used for simultaneous saccharification and fermentation of sugarcane slag paper pulp for alcohol production.

The invention relates to a normal-temperature fungus NJGZ-2 capable of degrading agricultural waste straw, belonging to the agricultural waste resource utilization technology. The class of the strain NJGZ-2 is named penicillium oxalicum, and the culture is collected in the China General Microbiological Culture Collection Center (CGMCC), with collection number CGMCC No.7527. Tests indicate that after the rice straw is inoculated with a spore suspension (1% 10<7> spores / mL), the weight loss ratio of the rice straw is 59.7% after 7 days of 170r / min liquid fermentation culture at 30 DEG C. In the fermentation crude enzyme extraction liquid, the activities of incision enzyme, beta-glucosidase, xylanase, beta-xylosidase and total cellulose are 113.3U / gds, 11.6U / gds, 1,439.7U / gds, 2.0U / gds and 13.8U / gds respectively. The crude enzyme liquid can hydrolyze low-concentration alkali-treated corncob to generate high-concentration reducing sugar (962.2mg of reducing sugar / gram of substrate). According to the invention, the agricultural waste straw is degraded by the microbial fermentation technology and is converted into organic fertilizer, therefore, the waste can be turned into wealth to protect the environment, and application prospects are broad.

The invention relates to a production method for producing a high-efficiency phosphors-resolving penicillium oxalicum agent with heavy metal tolerance characteristic by utilizing sweet potato starch waste water and waste mushroom residue slag, which belongs to the field of environmental biotechnology. The high-efficiency penicillium oxalicum is separated and screened from topsoil of a lead zinc ore at Huayuan county of the Xiangxi autonomous region in Hunan province in the laboratory, and has the characteristic of tolerating multiple metals. According to the method, the phosphors-resolving penicillium oxalicum agent with heavy metal tolerance characteristic is produced mainly by utilizing sweet potato starch waste water and waste mushroom residue slag as main culture medium materials through adopting a liquid-solid combined fermentation method. The production method for the penicillium oxalicum agent comprises six steps of culture medium preparation, strain activation, preparation of liquid seeds, liquid-state fermentation, solid-state fermentation and aftertreatment. According to the technical scheme, the production method provides a new approach for resource utilization of the high-concentration sweet potato starch waste water and waste mushroom residue slag, and the produced penicillium oxalicum agent has the characteristics of being environment-friendly and low in production cost, and the product also has the functions of obviously improving the quality of soil environment, and promoting the growth of plants.

The invention discloses a composite microbial agent and an application thereof to generation of fulvic acid by fermentation of brown coal with humic acid. The composite microbial agent comprises Bacillus subtilis whose preservation number is CGMCC No.12905, Trichoderma harzianum whose preservation number is CGMCC No.13762, and Penicillium oxalicum whose preservation number is CGMCC No. 13763. Theapplication method of the composite microbial agent is as follows: brown coal with humic acid and auxiliary materials are mixed, the composite microbial agent is added, water is added into a bed material, humidity is controlled at 60%, the materials are permitted to stand and decomposed for 7-14 days, water is added, uniform stirring is carried out, the materials are permitted to stand for 2 hoursat a room temperature, and supernatant is separated (containing fulvic acid). The agent has the beneficial effects that (1) the composite microbial agent is used for generating fulvic acid by fermentation of brown coal with humic acid, and compared with chemical approaches, the method does not produce pollutants and is environmentally friendly; (2) waste materials can be used as organic fertilizers, in order to realize green, high-efficient, and high-quality continuous utilization.

The invention discloses a Penicillum oxalicum, and applications thereof. The preservation name of the Penicillum oxalicum is Penicillum oxalicum SG4, and is preserved on 30th, April, 2015, in China Center For Type Culture Collection, in Wuhan university, Wuhan, China; and preservation number is CCTCCM 2015270. It is confirmed by experiments that the Penicillum oxalicum SG4 possesses strong inhibition effects on orange Penicillium citrinum, and can be used for preparing orange biological fresh-keeping agents; a novel direction is provided for development of novel biological fresh-keeping agents and orange biological preservation; and excellent economic and social benefits are achieved.

The invention belongs to the technical field of micro-organisms and soil fertilizers. Straw return to fields is a universally concerned agronomic measure for increasing soil fertility and increasing yields and efficiency in the world, both to achieve pollution-free processing of straws, but also to increase the soil fertility. However, if the decomposition of straws in the soil lasts for a long time, large soil void will be caused, to result production problems such as dead seedlings, and freeze injury are prone to generate, and to enable long-term survival of pests in the soil, bringing potential harm to agricultural production. The present invention provides an optimized preparation process for a fermentation broth by using maize straws as a main raw material, and Penicillium oxalicum (Accession No. CGMCCNo.4842) as a seed bacterium; and at the same time provides application methods of the fermentation broth in agricultural production. The fermentation broth can be used to accelerate decomposition of maize straws, and is suitable for straw return to field areas to promote maize straws decomposition and increase the soil fertility.

The invention discloses functional protein POX01167, an encoding gene thereof and application of the functional protein POX01167 and the encoding gene. The protein provided by the invention is obtained from penicillium oxalicum and is named as POX01167 protein. The protein provided by the invention is as the following (a1) or (a2): (a1) protein consisting of an amino acid sequence as shown in a sequence 1 in a sequence list; and (2) protein formed by performing substitution and / or deletion and / or addition of one or more amino acid residues on the amino acid sequence in the sequence 1, having the same function and derived from the sequence 1. A gene for encoding the POX01167 protein (POX01167 gene) also belongs to the protection range of the invention. Experiments prove that the functional protein POX01167 plays a critical role in regulating and controlling the expression process of a cellulase gene and a xylanase gene of the penicillium oxalicum, and has application potentiality of increasing the yield of the cellulase and the xylanase.

The invention provides a cellulose-degradation fungus, preparation of an inoculum and an application thereof. According to the invention, a strain is obtained from Mount Taishan woodland humus soil by artificial enrichment and screening; the strain is identified as penicillium oxalicum, which is named as penicillium oxalicum C2; the strain has good cellulose degradation capability, and high cellulose-production activity; after shake flask fermentation for 3 days, the filter paper cellulose activity is up to 132.26 U / mL; When used in silkworm excrement compost, the conidium inoculums prepared from the penicillium oxalicum C2 can increase the compost temperature, prolong the high temperature stage, facilitate water content reduction, promote organic matter decomposition and the increase of total nitrogen relative content, decrease the C / N ratio, raise the seed germination index, accelerate the composting process, improve decomposition efficiency and fertilizer quality; the preparation method of the inoculums is simple in process, low in cost, and suitable for industrial production.

The invention relates to an endogenous penicillium capable of antagonizing four fusarium fungi and application thereof. The endogenous oxalic acid penicillium A1 is obtained through artificial separating and screening from a healthy root system of continuously-cropped malus hupehensis. According to the endogenous penicillium, the growth speed is high, spores are propagated massively, most survival space can be occupied in a short time, the higher antagonizing effect on the main harmful bacteria (such as fusarium solani, fusarium proliferatum, fusarium moniliforme and fusarium oxysporum) which can cause continuous cropping obstacles of apples is achieved, the inhibition rate on the fusarium moniliforme is the highest, the inhibition rate on a corn culture medium is equal to or more than 80%, a certain degradation effect on pholrizin is achieved, and a theoretical basis and a technological base are supplied to biological prevention and control of the continuous cropping obstacles of the applies.

The invention discloses phosphorus-dissolving penicillium oxalicum with a broad spectrum acid production feature. The preservation number of the phosphorus-dissolving penicillium oxalicum is CCTCC No.M2019233. The phosphorus-dissolving penicillium oxalicum can grow well in a solid culture medium using calcium phosphate or iron phosphate as the only phosphorus source, the penicillium oxalicum canutilize insoluble phosphorus compounds as the growth substance after being cultured for 48 hours, and the penicillium oxalicum can grow a large number of colonies after being cultured for 3-7 days; ina liquid culture medium, the penicillium oxalicum can efficiently utilize calcium phosphate or iron phosphate with the maximum concentration being 50g / L to produce up to 2500mg / L of soluble phosphate. The penicillium oxalicum in a culture medium added with solid calcium phosphate can produce oxalic acid, formic acid, malic acid, acetic acid and lactic acid through metabolism, and the secretion quantity reaches up to 215.73mg / L. The microbial strain resource which is separated from a soil environment for the first time has a good application value in farmland soil phosphorus improving, soil fertility improving and soil heavy metal contamination comprehensive treatment promoting.

The invention belongs to the technical field of grain storage, and discloses a bacillus subtilis strain having broad-spectrum antagonism for main grain storage hazard-moulds, a preparation method of an antifungal metabolite of the bacillus subtilis strain, and application of the bacillus subtilis strain and the antifungal metabolite in grain storage. The strain is bacillus subtilis LM3403, and has a preservation number of CGMCC No. 9377. The bacillus subtilis LM3403 has a better inhibiting effect for the main grain storage hazard-moulds such as fusarium graminearum, fusarium moniliforme, aspergillus flavus, aspergillus niger, aspergillus glaucus, aspergillus fumigatus, penicillium oxalicum, penicillium expansum and mucor mucedo, and is wide in antibacterial spectrum, thus having a giant application potential. The antifungal metabolite of the bacillus subtilis LM3403 has an obvious inhibiting effect for the growth of the main grain storage hazard-moulds. The storage test results prove that after the antifungal metabolite of the bacillus subtilis LM3403 is added into stored grains, the growth of the moulds can be effectively inhibited in a grain storage process, so that the grain storage safety is guaranteed.

The present invention discloses Penicillium oxalicum EU2101 and applications of the Penicillium oxalicum EU2101 in preparation of a cellulase preparation and degradation of cellulose, wherein the Penicillium oxalicum EU2101 has the preservation number of CGMCC No.12769. The invention further provides a cellulase preparation prepared by using the Penicillium oxalicum EU2101, wherein the preparation method comprises: culturing the Penicillium oxalicum EU2101 at a temperature of 28-42 DEG C by using a culture medium, and collecting the fermentation product to obtain the cellulase preparation. According to the present invention, the experiment results prove that after the Penicillium oxalicum EU2101 is subjected to solid state fermentation culture, the extraction liquid has cellulase activities such as filter paper enzyme activity, CMC enzyme activity, Avicel enzyme activity, pNPC enzyme activity and pNPG enzyme activity, and can effectively hydrolyze the manihot esculenta residue being subjected to an alkali pretreatment to produce glucose, such that the application potential is provided in conversion and utilization of the manihot esculenta residue.

The invention relates to secalonic acid I originated from penicillium oxalicum and an application thereof in the preparation of drugs for preventing human esophagus cancer. Secalonic acid can inhibit the proliferation of human esophagus cancer cells. The structure of secalonic acid is represented in the description. Penicillium oxalicum IBPT-6 is cultured through fermentation to obtain the fermentation product; and secalonic acid is separated from the fermentation product and then purified. The experiment results show that secalonic acid can well inhibit the activity of human esophagus cancer cell EC9706. Secalonic acid can be used to prepare drugs for inhibiting the proliferation of human esophagus cancer cells and antitumor drugs, and can also be applied to the research on human esophagus cancer.

The invention belongs to the field of microbial fermentation and particularly relates to a penicillium oxallcum Po-5 strain capable of generating pectinase and a method for producing the pectinase by liquid fermentation. The penicillium oxallcum Po-5 strain is preserved in the China center for type culture collection on Dec14th in 2010 and the preservation number is CCTCC (China Center for Type Culture Collection) NO.M2010350. The strain with high yield of pectinase is selected from the natural environment and the pectinase is produced in a liquid fermentation mode so as to reduce the production cost of the pectinase, optimize the process and reduce the environment stress. The strain uses natural orange skins, rice brans and the like with low prices as a culture medium matrix, the activity of the pectinase obtained by fermentation can achieve 1.39*105U<min-1>< ml-1> and the penicillium oxallcum Po-5 strain belongs to a high-yield pectinase strain. The pectinase prepared by carrying out liquid fermentation on the Po-5 strain can keep good stability at the temperature of 30 to 50 DEG C when the pH (potential of hydrogen) value is equal to about 5.0.

The invention discloses penicillium oxalicum, a preparation method and an application thereof. The preparation method comprises the following steps: A) antagonistic fungi separation and purification: taking a healthy Camellia oleifera rhizosphere sample, adding the sample in a bottle filled with sterile water and glass bead, oscillating the bottle under a shaking table to prepare a fluid suspension; B) primary screening: employing a plate confrontation for screening the fungi for antagonism of Camellia pathogen, placing a pathogen cake and an antagonistic fungi cake on a flat center line, respectively keeping distances to the flat center by 1.5 cm, after constant temperature culture, screening the strains with a bacteriostasis diameter being greater than 8mm, and C) secondary screening: culturing the strains through primary screening by a liquid medium, centrifuging the strains and removing thallus, taking an aseptic filtrate and a disinfected PDA solid medium and mixing the aseptic filtrate and the disinfected PDA solid medium uniformly, pouring on plate, inoculating the Camellia pathogen cake to the center of the flat, and obtaining the strain penicillium oxalicum with strongest antagonistic action. The invention also provides an application of penicillium oxalicum for treating or preventing and treating oil camellia diseases, and the penicillium oxalicum has more than 90% of a bacteriostasis rate on a plurality of diseases such as oil tea anthracnose, oil tea canker and black spot.

The invention discloses Xiangyang radix ophiopogonis endophytic penicillium oxalicum penicillium fungus and application in steroid saponin preparation, and relates to the technical field of microorganisms. The problems that Xiangyang radix ophiopogonis steroid saponin is mostly directly from Xiangyang radix ophiopogonis extractives, and the Xiangyang radix ophiopogonis steroid saponin is limited in market delivery quantity due to the fact that Xiangyang radix ophiopogonis is low in steroid saponin content and high in saponin extraction cost are solved. The penicillium fungus is separated fromXiangyang radix ophiopogonis (Liriope spicata var.proliferaY.T.Ma) living earthnuts, it is determined as penicillium sp. EF050 through 18S rDNA sequence authentication, the penicillium fungus is preserved in the China Center for Type Culture Collection, the preservation date is 20th December, 2016, and the preservation number is CCTCC No:M2016766. By means of Xiangyang radix ophiopogonis producedin a Xiangyang radix ophiopogonis GAP base-the source area of Xiangyang radix ophiopogonis in Oumiao county, Xiangyang city, Hubei province, due to fermentation of Xiangyang radix ophiopogonis endophytic fungi strain liquid, the Xiangyang radix ophiopogonis steroid saponin is produced. The endophytic fungi are the important microorganism for searching for new resources of the Xiangyang radix ophiopogonis steroid saponin.

The invention relates to secalonic acid I derived from penicillium oxalicum and application of the secalonic acid I for preparing a medicine for preventing human colon cancer. The compound has a function of inhibiting human colon cancer cell proliferation. The structural formula of the secalonic acid I is as shown in the specification. Through fermentation culture of Penicillium oxalicum IBPT-6, a fermented substance is prepared, and the compound is separated and purified from the fermented substance. Tests show that the compound has relatively high anti-tumor activity upon human colon cancer cells HCT116. The compound can be used for preventing human colon cancer cell proliferation inhibition medicines or anti-tumor medicines for studying human colon cancer.

The invention discloses a fungus with straw degradation capability and application thereof in composting and relates to the technical field of biological environmental protection. The straw degradation fungus JSD-17 is penicillium oxalicum (Penicillium oxalicum) and has a preservation number of CGMCC No.7743; the straw degradation fungus is further used for fermenting and composting agricultural waste straw. The penicillium oxalicum disclosed by the invention not only has strong cellulose enzyme synthesis capability, but also has the capability of decomposing hemicellulose and pectin components in the straw. By adopting the fungus disclosed by the invention, laccase (Lac), lignin peroxidase (Lip) and manganese peroxidase (Mnp) can be synthesized; the degradation speed of lignin components in the straw can be greatly increased.

The invention discloses a penicillium oxalicum for reducing hexavalent chromium and a screening method thereof, and belongs to the technical field of microorganisms. The penicillium oxalicum is named as penicillium oxalicum SL2, and is stored in the China Center for Type Culture Collection on Oct. 22, 2014, and the preservation number of the penicillium oxalicum SL2 is CCTCC M 2014505. The bacterial strain can grow up in a PDA culture medium, in which the concentration of Cr (VI) is 2,000 mg / L, tolerates a NaCl saline solution of 100 g / L, and can completely reduce Cr (VI) of 357.9 mg / L in the culture medium within five days. The penicillium oxalicum for reducing the hexavalent chromium can be applied to treating Cr (VI) pollution in the environment.

The invention relates to secalonic acid I derived from penicillium oxalicum and application of the secalonic acid I for preparing a medicine for preventing human oral epidermoid carcinoma. The compound has a function of inhibiting human oral epidermoid carcinoma cell proliferation. The structural formula of the secalonic acid I is as shown in the specification. Through fermentation culture of Penicillium oxalicum IBPT-6, a fermented substance is prepared, and the compound is separated and purified from the fermented substance. Tests show that the compound has relatively high anti-tumor activity upon human oral epidermoid carcinoma cells KB. The compound can be used for preventing human oral epidermoid carcinoma cell proliferation inhibition medicines or anti-tumor medicines for studying human oral epidermoid carcinoma.

The invention discloses a preparation method of penicillium oxalicum and application of penicillium oxalicum in phosphate solubilizing, growth promoting and fusarium graminearum antagonism, and belongs to the technical field of environmental biology. The penicillium oxalicum MEM02 can inhibit germination of fusarium graminearum spores in soil and remarkably reduce infection of the fusarium graminearum spores to wheat, and meanwhile the penicillium oxalicum MEM02 can efficiently solubilize phosphorus and promote plant growth. Therefore, the penicillium oxalicum MEM02 has good application prospects in the aspect of developing new biopesticides or new biological control microbial agents.

The invention relates to a technology for producing feed-stage pectase by fermenting animal-source penicillium oxalicum obtained by being separated from a goose body, which belongs to the field of microorganism fermentation. An enzyme preparation with high enzyme activity, long storage period, good keeping quality, stable ingredient percentage content and high purity is produced by solid fermentation. The total enzyme activity of the produced pectase enzyme preparation reaches 10123.22U*g; the enzyme activity of polygalacturonic acid is 5101.23U*g which is 50 percent of the total enzyme activity; the pectinesterase activity is 6986.52U*g, which is 69 percent of the total enzyme activity; and the pectin lyase activity is 0.56U*g, which is 0.005 percent of the total enzyme activity. When 0.2 percent of the pectase enzyme preparation is added into the animal daily food, the digesting rate of coarse fiber (CF) is increased by 17.04 percent, the digesting rate of neutral detergent fiber (NDF) is increased by 12.15 percent, the digesting rate of acid detergent fiber (ADF) is increased by 13.95 percent, the net protein utilizing rate (NPU) is increased by 9.53 percent, and the apparent digestibility of Ca and P are respectively increased by 7.04 percent and 8.38 percent than those of a contrast group. The technology solves the key producing technical problem of the unconfirmed enzyme ingredient and the content of the enzyme preparation used for producing the feed of compound bacteria fermentation, and provides a new technology for the reasonable compatibility and application of the enzyme preparation of different ingredients of the pectase.

Belonging to the technical field of microorganisms, the invention in particular relates to a Penicillium oxalicum FH6 strain, a screening method and application thereof. The preservation number of the Penicillium oxalicum FH6 strain is CGMCC No.8270. The method for applying the strain to enzymolysis of enteromorpha includes: subjecting a cryopreserved FH6 strain to activation culture in a Martin slant culture medium, then picking a full ring and placing it in a Martin liquid culture medium to perform shaking culture for 24-28h, then taking 1-2mL of a bacterial solution to conduct shaking culture for 5-7d in a sterilized cellulose liquid fermentation medium; then carrying out low temperature centrifugation on the fermented liquid, taking the supernatant as a crude enzyme solution or filtering the fermented liquid, and taking the filtrate as a crude enzyme solution; and reacting the crude enzyme solution with clean enteromorpha powder at 45-55DEG C. The Penicillium oxalicum FH6 strain has a cellulose production ability, can effectively degrade cellulose materials in enteromorpha into reducing sugar. Also, the production cost is low, the equipment is simple, and the reaction is mild, thus being suitable for large-scale industrial production.

The invention discloses a composite microbial agent for a coal gangue artificial ecological matrix, which comprises five actinomycetes, streptomyces thermoviolaceus, streptomyces thermodiastaticus, streptomyces thermocarboxydus, streptomyces albidoflavus and streptomyces thermovulgaris, and one bacterium is pseudomonas sp. and one mould is penicillium oxalicum. The preparation process of the composite microbial agent comprises the following four steps: activating strains, preparing a seed solution, preparing a liquid composite microbial inoculum and preparing a solid microbial inoculum. According to the technology, the problems that microorganisms in the coal gangue are lacked, and nutrients are not beneficial to plant absorption and utilization are solved, a new way is provided for resource utilization of the coal gangue and landscaping waste, and the preparation has wide application prospects in the aspects of treatment, resource utilization and the like of the coal gangue.

The invention discloses a method for returning crop stalks to the field by microbial fermentation. The method for returning crop stalks to the field comprises the following steps: crop stalks treatment, applying of farm manure, fermenting treatment and cropland application. According to the method for returning crop stalks to the field by microbial fermentation, penicillium oxalicum NJGZ-2 bacteria as microorganisms and a biogas digester are combined to ferment and degrade the crop stalks which are returned to the field directly, environmental pollution caused by crop stalk waste can be reduced, furthermore, the fermenting time can be shortened, fermenting temperature can be increased effectively, the purpose of thoroughly killing some pathogens such as insect ova and vectors is achieved, degraded soil can further be repaired, development of green agriculture is facilitated, and sustainable development of the agriculture is promoted.