Composite microbial agent and application thereof to generation of fulvic acid by fermentation of brown coal with humic acid

A compound microbial agent and humic acid technology, applied in the field of microorganisms, can solve the problems of low thermal efficiency of lignite combustion, pollute the environment, secondary pollution, etc., and achieve high-quality sustainable utilization

Active Publication Date: 2018-03-20
QINGDAO YIBAINONG FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is rich in lignite resources, but the thermal efficiency of lignite combustion is low, and it is easy to cause environmental pollution
The traditional chemical method to extract fulvic acid...

Method used

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  • Composite microbial agent and application thereof to generation of fulvic acid by fermentation of brown coal with humic acid
  • Composite microbial agent and application thereof to generation of fulvic acid by fermentation of brown coal with humic acid
  • Composite microbial agent and application thereof to generation of fulvic acid by fermentation of brown coal with humic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Mix the humic acid lignite and the compound microbial agent according to the volume ratio of 7:3, add water to the base material after mixing, control the humidity at about 60%, and then let it stand for 7 days to decompose, and then according to the volume of material: water = 1:50 Then add water, stir evenly, let stand at room temperature for 2h, and separate the supernatant.

[0067] After infrared spectroscopy analysis, refer to figure 1 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

[0068] The supernatant containing fulvic acid can be used directly, or can be dried to obtain fulvic acid powder, and the residue can be further extracted or directly used as fertilizer.

Embodiment 2

[0070] Mix humic acid lignite and auxiliary material wheat bran, and then add compound microbial agent. The volume ratio of humic acid lignite, wheat bran, and compound microbial agent is 8:1:1. After mixing, add water to the base material to control the humidity At about 60%, then let it stand for 7 days to decompose, then add water according to the volume ratio of material: water = 1:50, stir evenly, stand at room temperature for 2 hours, and separate the supernatant.

[0071] After infrared spectroscopy analysis, refer to figure 2 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

Embodiment 3

[0073] Mix humic acid lignite and auxiliary material soybean meal, then add compound microbial agent, the volume ratio of humic acid lignite, soybean meal, and compound microbial agent is 7:1:2, add water to the bottom material after mixing, and control the humidity at 60 %, then let it stand for 7 days to decompose, then add water according to the volume ratio of material: water = 1:50, stir evenly, stand at room temperature for 2 hours, and separate the supernatant.

[0074] After infrared spectroscopy analysis, refer to image 3 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

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Abstract

The invention discloses a composite microbial agent and an application thereof to generation of fulvic acid by fermentation of brown coal with humic acid. The composite microbial agent comprises Bacillus subtilis whose preservation number is CGMCC No.12905, Trichoderma harzianum whose preservation number is CGMCC No.13762, and Penicillium oxalicum whose preservation number is CGMCC No. 13763. Theapplication method of the composite microbial agent is as follows: brown coal with humic acid and auxiliary materials are mixed, the composite microbial agent is added, water is added into a bed material, humidity is controlled at 60%, the materials are permitted to stand and decomposed for 7-14 days, water is added, uniform stirring is carried out, the materials are permitted to stand for 2 hoursat a room temperature, and supernatant is separated (containing fulvic acid). The agent has the beneficial effects that (1) the composite microbial agent is used for generating fulvic acid by fermentation of brown coal with humic acid, and compared with chemical approaches, the method does not produce pollutants and is environmentally friendly; (2) waste materials can be used as organic fertilizers, in order to realize green, high-efficient, and high-quality continuous utilization.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its application, in particular to a composite microbial bacterial agent and its application in fermenting humic acid lignite to produce fulvic acid, belonging to the technical field of microorganisms. Background technique [0002] Fulvic acid can be widely used in industry, agriculture, medicine, animal husbandry and other aspects. Taking the application in agriculture as an example, fulvic acid can promote plant growth, control the opening of crop leaf stomata, reduce transpiration, fight drought, improve crop stress resistance, increase yield and improve crop quality. [0003] At present, the production of fulvic acid mainly adopts chemical methods, mainly extracted from weathered coal. my country has abundant lignite resources, but the thermal efficiency of lignite combustion is low, and it is easy to cause environmental pollution. The traditional chemical method to extract fulvic acid from ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P1/02C12P1/04C12R1/125C12R1/80C12R1/885
CPCC12N1/14C12N1/20C12P1/02C12P1/04C12P39/00C12N1/145C12N1/205C12R2001/125C12R2001/80C12R2001/885
Inventor 李文正
Owner QINGDAO YIBAINONG FERTILIZER CO LTD
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