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A compound microbial agent and its application in fermenting humic acid lignite to produce fulvic acid

A technology of compound microbial inoculants and humic acid, applied in the field of microorganisms, can solve the problems of low thermal efficiency of lignite combustion, poor fulvic acid activity, environmental pollution, etc., and achieve the effect of high-quality sustainable utilization

Active Publication Date: 2021-03-12
QINGDAO YIBAINONG FERTILIZER CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is rich in lignite resources, but the thermal efficiency of lignite combustion is low, and it is easy to cause environmental pollution
The traditional chemical method to extract fulvic acid from lignite not only has low extraction efficiency, but also has poor activity and low flocculation limit, and also produces secondary pollutants that pollute the environment during the extraction process

Method used

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  • A compound microbial agent and its application in fermenting humic acid lignite to produce fulvic acid
  • A compound microbial agent and its application in fermenting humic acid lignite to produce fulvic acid
  • A compound microbial agent and its application in fermenting humic acid lignite to produce fulvic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Mix the humic acid lignite and the compound microbial agent according to the volume ratio of 7:3, add water to the base material after mixing, control the humidity at about 60%, and then let it stand for 7 days to decompose, and then according to the volume of material: water = 1:50 Then add water, stir evenly, let stand at room temperature for 2h, and separate the supernatant.

[0067] After infrared spectroscopy analysis, refer to figure 1 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

[0068] The supernatant containing fulvic acid can be used directly, or can be dried to obtain fulvic acid powder, and the residue can be further extracted or directly used as fertilizer.

Embodiment 2

[0070] Mix humic acid lignite and auxiliary material wheat bran, and then add compound microbial agent. The volume ratio of humic acid lignite, wheat bran, and compound microbial agent is 8:1:1. After mixing, add water to the base material to control the humidity At about 60%, then let it stand for 7 days to decompose, then add water according to the volume ratio of material: water = 1:50, stir evenly, stand at room temperature for 2 hours, and separate the supernatant.

[0071] After infrared spectroscopy analysis, refer to figure 2 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

Embodiment 3

[0073] Mix humic acid lignite and auxiliary material soybean meal, then add compound microbial agent, the volume ratio of humic acid lignite, soybean meal, and compound microbial agent is 7:1:2, add water to the bottom material after mixing, and control the humidity at 60 %, then let it stand for 7 days to decompose, then add water according to the volume ratio of material: water = 1:50, stir evenly, stand at room temperature for 2 hours, and separate the supernatant.

[0074] After infrared spectroscopy analysis, refer to image 3 , the supernatant at 1720cm -1 、1620cm -1 、 1400cm -1 、1250cm -1 There is an absorption peak at the place, which is consistent with the absorption spectrum of fulvic acid, confirming that the supernatant contains fulvic acid.

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Abstract

The invention discloses a composite microbial agent and its application in fermenting humic acid lignite to produce fulvic acid, wherein the composite microbial agent consists of Bacillus subtilis with a preservation number of CGMCC NO.12905, and a preservation number of CGMCC NO. .13762 Trichoderma harzianum and Penicillium oxalicum with preservation number CGMCC NO.13763; the application method of the compound microbial inoculum is as follows: mix humic acid lignite with auxiliary materials, then add the aforementioned compound microbial inoculum, Add water, control the humidity at 60%, let stand for 7-14 days, then add water, stir evenly, let stand at room temperature for 2 hours, and separate the supernatant (containing fulvic acid). The benefits of the present invention are: (1) use the composite microbial bacterial agent provided by the present invention to ferment humic acid lignite to produce fulvic acid, which does not produce pollutants and is more environmentally friendly compared with chemical methods; (2) waste can be used as Organic fertilizers realize green, efficient and high-quality sustainable utilization.

Description

technical field [0001] The invention relates to a microbial bacterial agent and its application, in particular to a composite microbial bacterial agent and its application in fermenting humic acid lignite to produce fulvic acid, belonging to the technical field of microorganisms. Background technique [0002] Fulvic acid can be widely used in industry, agriculture, medicine, animal husbandry and other aspects. Taking the application in agriculture as an example, fulvic acid can promote plant growth, control the opening of crop leaf stomata, reduce transpiration, fight drought, improve crop stress resistance, increase yield and improve crop quality. [0003] At present, the production of fulvic acid mainly adopts chemical methods, mainly extracted from weathered coal. my country has abundant lignite resources, but the thermal efficiency of lignite combustion is low, and it is easy to cause environmental pollution. The traditional chemical method to extract fulvic acid from ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14C12P39/00C12P1/02C12P1/04C12R1/125C12R1/80C12R1/885
CPCC12N1/14C12N1/20C12P1/02C12P1/04C12P39/00C12N1/145C12N1/205C12R2001/125C12R2001/80C12R2001/885
Inventor 李文正
Owner QINGDAO YIBAINONG FERTILIZER CO LTD
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