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Phosphorus dissolvable penicillium oxalicum P8

A technology of phosphate-dissolving Penicillium oxalicum and Penicillium oxalicum, applied in the field of microbial inoculum, to achieve the effects of reducing the amount of phosphate fertilizer, increasing crop yield, and improving the utilization rate of phosphate fertilizer

Inactive Publication Date: 2010-02-10
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many properties of Penicillium oxalicum P8 have not been reported in previous studies

Method used

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  • Phosphorus dissolvable penicillium oxalicum P8
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  • Phosphorus dissolvable penicillium oxalicum P8

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] The preparation of embodiment 1 Penicillium oxalicum strain P8 and its phosphate-dissolving bacterial agent

[0064] 1. Composition of medium ( / L):

[0065] Soluble starch 10g

[0066] 10g soybean cake powder

[0067] 5 g corn flour

[0068] Glucose 1 g

[0069] 10 grams of sucrose

[0070] Yeast powder 5 grams

[0071] (NH 4 ) 2 SO 4 3 grams

[0072] K 2 HPO 4 0.2 grams

[0073] NaCl 2.5g

[0074] MgSO 4 ·7H 2 O 0.1 g

[0075] CaCO 3 0.5 grams

[0076] FeSO 4 0.001g

[0077] Boric acid (1%) 1ml

[0078] pH 7.0

[0079] 2. Training process:

[0080] Strain P8 was inoculated on potato sucrose (PDA) medium, cultured at 28-30°C for 48h, then inserted into a 500mL Erlenmeyer flask, cultured at 30°C, 180r / min for 36h. Then insert in the 15L seed tank by 1% inoculum amount, at 220r / min, pH7.0, under aeration 0.6vvm, after cultivating 24h, then pack in the fermenter of 100L by 5% inoculum amount, in 220r / min, pH 7.0, venti...

experiment example 1

[0081] The phosphorus-dissolving ability of Experimental Example 1 Penicillium oxalicum P8

[0082] 1. Phosphorus dissolving effect under solid culture conditions

[0083] In selective modified Pikovskaya medium (medium composition (g / L): (NH 4 ) 2 SO 4 0.5, NaCl 0.2, KCl 0.2, MgSO 4 ·7H 2 O 0.03, MnSO 4 0.03, FeSO 4 0.003, yeast powder 0.5, glucose 10.0, phosphorus source 5.0), the soil samples collected in the Shijiazhuang suburban grassland were diluted step by step, with 10% of the soil solution -4 The plates were coated, and phosphorus-dissolving microorganisms were isolated. According to the size of the phosphorus-dissolving circle, 70 strains of phosphorus-dissolving bacteria and fungi were preliminarily isolated. Then, on the selective medium with tricalcium phosphate as the sole phosphorus source, the phosphorus-dissolving ability of these strains was further compared, and the phosphorus-dissolving ability of fungi was greater than that of bacteria. Finally...

experiment example 2

[0112] Experimental example 2 Penicillium oxalicum P8 promotes crop yield increase

[0113]Under potted conditions, three kinds of calcareous soils with different phosphorus levels were used using peanuts, corn, and rapeseed (Soil No. 1 was taken from Daxing District, Beijing, the soil was light loamy fluvo-aquic soil, and the available phosphorus (P) was 13.5 μg / g ; No. 2 soil was taken from dry and thin land in Wuqiang County, Hebei Province, and the soil was medium loamy fluvo-aquic soil, with available phosphorus (P) 4.7 μg / g; No. 3 soil was taken from Changping District, Beijing, and the soil was medium loamy dehumidified soil , Abandoned land for many years, available phosphorus (P) μg / g 5.2), using the method of soil depletion continuous planting, studied the effect of Penicillium oxalicum P8 on the absorption of soil phosphorus and crop growth, the results are shown in Table 6 and 7 , 8 shown.

[0114] Table 6 corn is inoculated with the biological yield (g / pot) of Pe...

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Abstract

The invention provides a phosphorus dissolvable penicillium oxalicum P8, which has the function of dissolving various kinds of indissolvable phosphorus in soil, is suitable for red soil in the South,black soil, drab soil and moisture soil in the North and other types of soil, and has various functions of improving the crop yield, activating the indissolvable phosphorus in the soil, preventing thesoil chemical fixation of phosphorus fertilizers and improving the utilization efficiency of the phosphorus fertilizers.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents, and in particular relates to a phosphate-dissolving Penicillium oxalicum (Penicillium oxalicum) P8, a phosphorus-dissolving bacterial agent containing the bacteria and uses thereof. Background technique [0002] The threat of severe phosphorus deficiency worldwide is coming to human beings, and the service life of natural phosphate rocks on the earth is only 50 years. my country has a large demand for phosphate fertilizers and a serious shortage of phosphate rock resources. [0003] About 90% of the phosphate fertilizer applied to the soil is fixed by the soil, forming compounds such as calcium phosphate, iron phosphate, and aluminum phosphate with extremely low solubility, which have entered the ranks of insoluble phosphorus resources in the soil. More than 90% of soil phosphorus is inorganic phosphorus, and more than 50% of insoluble phosphorus in northern soil exists in the form of c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C05F11/08C12R1/80
Inventor 范丙全龚明波
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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