25 results about "Phosphorus deficiency" patented technology

The invention relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 to improvement of low-phosphorus stress tolerance of plants. Research finds that under the condition of low phosphorus stress, the expression level of the GmAP2 gene in plants is remarkably increased. The GmAP2 gene is subjected to heterologous overexpression in arabidopsis thaliana, so that stress on plants under a low-phosphorus condition can be effectively relieved, the fresh weight and lateral root length of the arabidopsis thaliana can be remarkably increased, and the growth of the arabidopsis thaliana plants is promoted. In addition, under the low-phosphorus condition, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptom of the soybean, improve the absorption of phosphorus nutrients by plants and promote the growth of plant roots and leaves. Therefore, the GmAP2 gene plays a very important role in dealing with the phosphorus starvation stress environment by the plant.

The present application relates to the technical field of forest seedling and intelligent agriculture, and specifically discloses an intelligent monitoring and nutrient regulation system for sprouting seedlings of fragrant fruit trees, which calculates the red edge position blue shift deviation by collecting the multispectral image of the crown layer of the sprouting seedlings to identify the phosphorus deficiency phenotype; determines the spatial phosphorus deficiency and obtains the root zone reference conductance value by combining the root zone and surface layer phosphorus concentration spatial comparison detection; determines the irrigation interval according to the difference between the reference conductance value and the critical value of fertilizer damage, and precisely applies high-phosphorus water-soluble fertilizer by using pressure-compensating drippers with low flow; after irrigation, the irrigation time is dynamically shortened and the target conductance value is reduced according to the root zone conductance rise amplitude, and after continuous execution for three days, the irrigation is terminated when the blue shift deviation and the color of the newly grown leaves both meet the recovery conditions; the present application realizes early identification and closed-loop feedback regulation of the spatial phosphorus deficiency of the sprouting seedlings of fragrant fruit trees, avoids fertilizer damage and root rot, and improves the accuracy and safety of nutrient regulation.

Plants having modified expression or activity of a protein kinase polypeptide are provided. Methods for modifying expression or activity of the protein kinase polypeptide include genome editing to modify the transcription regulatory region or sequence encoding the protein kinase polypeptide as well as transformation with a heterologous polynucleotide encoding the protein kinase polypeptide. Plants containing the modifications have improved phosphorus use efficiency, increased tolerance to phosphorus deficiency, and improved yield in phosphorus deficient conditions.

The application relates to the technical field of plant genetic engineering, and discloses application of a soybean low-phosphorus-tolerant gene GmAP2 in improving the low-phosphorus-stress tolerance of plants. The application finds that, under low-phosphorus stress, the expression level of the GmAP2 gene in plants is significantly up-regulated. By heterologously overexpressing the GmAP2 gene in Arabidopsis, the stress suffered by plants under low-phosphorus conditions can be effectively relieved, and the fresh weight and lateral root length of the Arabidopsis can be significantly increased, so that the growth of the Arabidopsis plant is promoted. In addition, under low-phosphorus conditions, overexpression of the GmAP2 gene can improve the phosphorus deficiency symptoms of soybean, improve the absorption of phosphorus nutrients by plants, and promote the growth of the roots and leaves of the plants. Therefore, the GmAP2 gene plays a very important role in the response of plants to a phosphorus-starvation stress environment.

The present invention belongs to the field of microbial technology, and particularly relates to a phosphate-solubilizing Aspergillus terreus and its application in drought-resistant probiotics; the Aspergillus terreus of the present invention is obtained by screening the rhizosphere soil of pepper, and the Aspergillus terreus CY-05 ( Aspergillus terreus var. fluffy ) is a growth-promoting microbial strain with disease resistance, high yield of terreus ketone and phosphate solubilization ability, and is an excellent strain with strong survival ability and multiple functions such as improving crop drought tolerance. The present invention also provides a probiotic agent obtained by solid-state fermentation of Aspergillus terreus CY-05. The application of this agent can increase the tolerance of crops to drought, improve the yield and survival ability of crops under drought stress conditions, promote the conversion and utilization of insoluble phosphorus, and prevent and control the occurrence of root soil-borne diseases. Strain CY-05 is an indigenous fungus in the rhizosphere of pepper. It is non-toxic and harmless to humans and animals, does not cause environmental pollution, and helps to solve the problems of water shortage and phosphorus deficiency faced by agricultural production in large semi-arid planting areas. It has important economic significance.

The invention belongs to the technical field of microorganisms, and particularly relates to a complex microbial inoculant for relieving phosphorus deficiency in the seedling stage of corn covered with straw and application of the complex microbial inoculant. Bacillus subtilis, saccharomycetes and pseudomonas fluorescens are compounded into the complex microbial inoculant, saccharomycetes secrete IAA and siderophores to promote the root development of seedlings, through domestication culture, the adaptability of the microbial inoculum in a straw-covered low-temperature environment is enhanced, the activation capacity on phosphorus is improved, the phosphorus nutrition level is synergistically improved in cooperation with bacillus subtilis, pseudomonas fluorescens can enhance the resistance of the seedlings to low-temperature stress, and the survival rate of the seedlings is increased. Soil-borne pathogenic bacteria which cannot be effectively inhibited by some soil florae are specifically inhibited, the influence of harmful bacteria on the activation effect of phosphorus is reduced, the three strains are matched to remarkably improve the content of rapidly available phosphorus in soil, reduce the incidence rate of phosphorus deficiency symptoms and improve the stress resistance and growth indexes in the corn seedling stage, and the compound microbial agent is suitable for a straw covering cultivation mode.

The invention discloses a rhizosphere phosphate-solubilizing bacterium for activating insoluble inorganic phosphorus in southern red soil and combined application of prebiotics of the rhizosphere phosphate-solubilizing bacterium. The invention provides rhizosphere phosphorus solubilizing Burkholderia capable of activating insoluble inorganic phosphorus in southern red soil, and also provides prebiotic oxalic acid capable of specifically enhancing the phosphorus solubilizing capacity of the strain. By directly applying the strain, insoluble phosphate (iron phosphate) in red soil can be activated, available phosphorus can be released, and phosphorus deficiency stress of red soil crops can be relieved; the combined application of the strain and prebiotics can further improve the rhizosphere phosphate solubilizing and crop growth promoting effects. The invention has important significance for developing a functional microbial preparation for enhancing the phosphorus activation capability of the crop rhizosphere under the low phosphorus stress and promoting the growth and development of the crop.

The invention discloses a biochar-based fertilizer and a preparation method thereof, and relates to the technical field of fertilizer.The biochar-based fertilizer is prepared by firstly using livestock and poultry manure as a raw material, the biochar is mixed with molten urea and diammonium phosphate, so that the biochar and the fertilizer are mixed and loaded together, and on the basis, the biochar-based fertilizer is prepared. Starch-based water-absorbent resin is also prepared to serve as a binder of the biochar-based fertilizer, and quaternary ammonium salt groups are introduced into the starch-based water-absorbent resin, so that in an acidic environment caused by acid production due to phosphorus deficiency of wheat, the quaternary ammonium salt groups in molecular chains of the resin can generate strong electrostatic repulsion due to positive electricity, pores of a cross-linked network are enlarged, and the biochar-based fertilizer is prepared. And when water permeation and swelling are accelerated, a larger channel can be provided for diffusion of fertilizer molecules, so that the growth conditions of crops are improved according to actual needs of the crops, and the growth of the crops is improved.

The invention relates to the technical field of plant genetic engineering, and discloses a soybean GmPHR2 gene and an application thereof in regulating the tolerance of soybeans to low phosphorus stress. The nucleotide sequence of the CDS region of the GmPHR2 gene is as shown in SEQ ID NO. 1. Through cloning and functional analysis of the GmPHR2 gene, it is found that the growth ability and phosphorus utilization efficiency of plants in a low-phosphorus environment are remarkably improved through overexpression of the GmPHR2 gene. In order to further verify the function of the gene GmPHR2, the gene GmPHR2 is subjected to heterologous overexpression in soybean, and the result shows that the tolerance of an overexpression plant under low-phosphorus stress is obviously enhanced, the fresh weight and lateral root length of the plant are obviously increased, and the growth condition is obviously improved. The research provides important gene resources and theoretical basis for low-phosphorus-tolerant breeding of crops, is beneficial to cultivation of new soybean varieties adapting to low-phosphorus soil, and improves the production potential of soybeans in a phosphorus-deficient environment.

The application discloses a synthesis method of a biological source calcium oxalate mineral composite material for selective holding of lead ions and application thereof. The method inoculates a fungal spore suspension in a soluble calcium phosphate deficient stress liquid culture medium, and after the fungus fully releases insoluble phosphorus, a calcium oxalate mineral composite material is obtained by separation. The synthesized calcium oxalate mineral composite material has the characteristics of strong selectivity for lead ions, good adsorption effect and difficulty in desorption, and can selectively adsorb lead from mixed heavy metal waste liquid. The raw materials used in the application are easy to obtain and low in cost. The biological synthesis process is economic, environmental protection, simple in operation, the obtained mineral composite material is strong in acid resistance, storage and use are simple, and has a good application prospect for selective recovery of lead resources in composite heavy metal contaminated wastewater.

The invention belongs to the technical field of gene engineering, and provides a rice transcription factor OsbHLH18 responding to phosphorus deficiency stress and application thereof, the transcription factor OsbHLH18 is subjected to overexpression to be applied to increase of the phosphorus content of rice, an expression mode of the OsbHLH18 is explored, the effect of an overexpression OsbHLH18 carrier on rice growth is evaluated, and the result shows that the OsbHLH18 is a transcription factor positioned on a cell nucleus, and the OsbHLH18 is a transcription factor positioned on a cell nucleus. The transcription activity is realized; osbHLH18 is subjected to phosphorus deficiency induced expression on leaves and roots of rice, and the phosphorus deficiency induced expression is faster and higher in response to the roots; the plant height of the overexpressed OsbHLH18 rice is higher than that of a wild type, the overexpressed OsbHLH18 can regulate and control expression of downstream acid phosphatase genes, then the activity of acid phosphatase of root systems and root surfaces is improved, the phosphorus deficiency stress adaptability of the rice is relieved by degrading organic phosphorus, and the phosphorus content of the rice is improved.

The invention discloses application of a corn ZmMYB80 gene in regulation and control of corn root system development and low phosphorus stress resistance. The polynucleotide sequence of the corn ZmMYB80 gene is shown as SEQ ID No: 1, and the protein sequence of the corn ZmMYB80 gene is shown as SEQ ID No: 2. Under normal phosphorus supply and phosphorus deficiency conditions, compared with a wild type, ZmMYB80 overexpression remarkably increases the total root length of corn, and the decreasing amplitude of the total root length of the wild type under the low-phosphorus condition is remarkably higher than that of an overexpression strain. Under low-phosphorus stress, the biomass of the overground part of the wild type is reduced by 30%; compared with the prior art, the decrease amplitude of the OE # 1 and the OE # 2 of the ZmMYB80 overexpression strain is only 12% and 22%, which indicates that the ZmMYB80 overexpression material has stronger tolerance to low phosphorus stress. The discovery of the invention lays a foundation for cultivating a new corn variety for regulating and controlling corn root development and resisting low phosphorus stress.

The invention discloses an application of a GmASR2 gene in regulation and control of plant phosphorus nutrition. The relation between the soybean ASR transcription factor GmASR2 gene and the important nutrient element phosphorus is defined, it is proved that the gene participates in the low-phosphorus adaptation mechanism of plants, and the fact that the GmASR2 gene participates in soybean phosphorus nutrition regulation and control is disclosed for the first time. Researches show that the GmASR2 gene is obviously subjected to up-regulation expression under low phosphorus stress in roots and leaves, plant growth and phosphorus nutrition absorption are promoted by overexpression of the GmASR2 gene, and plant growth and phosphorus nutrition are positively regulated; under low phosphorus stress, the overexpression of the GmASR2 gene can improve the phosphorus deficiency symptom of the plant, improve the absorption of phosphorus nutrients and the accumulation of biomass of the plant, significantly promote the growth of the root system, and increase the phosphorus content and chlorophyll content of the plant. The GmASR2 gene plays an important role in improving plant phosphorus efficiency, promoting plant growth under low-phosphorus stress and increasing phosphorus content, and provides an important gene resource for plants to adapt to low-phosphorus environmental stress.

The invention discloses an application of a soybean PSK oligopeptide gene GmPSK alpha 14 in regulation and control of soybean growth and phosphorus nutrition thereof. The invention provides new application of the GmPSK alpha14 gene, and research shows that the GmPSK alpha14 gene can respond to low-phosphorus stress, is obviously subjected to up-regulation expression of low-phosphorus stress in soybean roots and leaves, and participates in regulation and control of soybean phosphorus nutrition and growth under low-phosphorus stress; the GmPSK alpha14 gene is cloned to prepare a recombinant vector, the recombinant vector is transformed into soybean for overexpression, it is shown that the GmPSK alpha14 gene is capable of regulating plant phosphorus nutrition, under the condition of phosphorus nutrient deficiency, overexpression of the GmPSK alpha14 gene can improve the phosphorus deficiency symptom of soybean, growth of soybean and soybean root systems can be remarkably promoted, absorption of soybean to phosphorus nutrient and accumulation of biomass can be improved, and the yield of the soybean can be increased. The plant phosphorus content is increased, the soybean growth is regulated, and more gene resources are provided for the plants to adapt to environmental low-phosphorus stress.

The application discloses Arthrobacter NJAU-OF121, which is classified and named as Arthrobacter nitrophenolicus and has a preservation number of CGMCC NO.34968. The application also discloses an acid-resistant aluminum growth-promoting biochar-based microbial agent, and a preparation method and application thereof. The Arthrobacter NJAU-OF121 can grow under acidic conditions (pH 4.5) and has the performances of IAA production, phosphorus dissolution and aluminum toxicity resistance under acidic conditions (pH 4.5), thereby providing a new strain resource for the production capacity improvement of acidic soil. In the acid-resistant aluminum growth-promoting biochar-based microbial agent, the survival number of functional bacteria is significantly improved, and the agent has more excellent effects of promoting the growth of rape (including relieving the aluminum toxicity and phosphorus deficiency of rape) and increasing yield (rape seed yield), thereby providing an effective guarantee for the high-yield cultivation of rape on acidic soil.

The invention discloses a Pseudomonas aeruginosa DT-6 and application thereof, and belongs to the technical field of microorganisms. According to the invention, three phosphate-solubilizing bacteria JX-5, JD-7 and DT-6 are screened out, the three phosphate-solubilizing bacteria are determined to have relatively strong dissolving capacity on organophosphorus lecithin, and the DT-6 strain has the most obvious effect, so that the DT-6 is selected as a test strain and is subjected to biological preservation, and the preservation number is CCTCC NO: M2024310. Furthermore, the metabolic regulation and control mechanism of the DT-6 on the cabernet sauvignon seedlings under the condition that available phosphorus in soil is deficient is deeply researched, an efficient local strain resource is provided for improving the utilization of the available phosphorus in the soil, and a theoretical basis and a practical reference are also provided for promoting green agriculture development and ecological environment protection.

The invention discloses a phosphate-solubilizing bacterium WY1-3, a microbial inoculum and application of the phosphate-solubilizing bacterium WY1-3. The strain is named as Burkholderia sp. In taxonomy, the strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 21, 2025, and the preservation number is CGMCC No.35299. The invention further discloses a preparation method of the Burkholderia sp. Through verification, the phosphate-solubilizing bacterium WY1-3 has better capability of improving the phosphorus effectiveness of calcareous purple soil, can effectively repair the phosphorus deficiency obstacle of the calcareous purple soil, can obviously increase the yield of rapeseeds and reduce the erucic acid content of the rapeseeds, and fills up the research blank of the phosphate-solubilizing bacterium in the aspect of reducing the erucic acid content of the rapeseeds. The strain has an application prospect in the field of calcareous purple soil rape planting, can improve the yield and quality of rape in calcareous purple soil, and also has an application prospect in soil optimization and remediation.

The invention relates to a method for protecting different types of plants against abiotic stress, increasing stress tolerance in plants, promoting growth and / or increasing nutritional efficiency, based on the use of a microorganism of the species Penicillium melinii and strains thereof. Also claimed is the use of a microorganism of the species Penicillium melinii and / or strains of same to protect plants from and / or increase the tolerance thereof to different types of abiotic stress, such as an absence of phosphorus, low availability or excess phosphorus, light stress, thermal stress, water stress, toxicity stress, salt stress and nutritional deficiency.

The application belongs to the technical field of biological gene engineering, and particularly relates to application of an OsSCL28 gene in regulating phosphorus deficiency stress tolerance of rice. The application knocks out the rice gene OsSCL28 from target rice, and the obtained transgenic rice has higher tolerance under low-phosphorus conditions, thereby providing guarantee for improving plant tolerance to phosphorus deficiency and cultivating new rice varieties suitable for phosphorus-poor soil. Meanwhile, knocking out the OsSCL28 gene can regulate morphological characteristics of rice. The method for preparing phosphorus-deficiency-tolerant rice based on gene editing technology disclosed by the application lays a foundation for regulation of rice agronomic traits and breeding of rice phosphorus absorption stress.

The invention relates to a method for improving the content of dencichine in panax notoginseng cells through phosphorus deficiency culture, and belongs to the technical field of panax notoginseng cell culture.The method is achieved by culturing panax notoginseng embryonic calluses under the phosphorus deficiency condition, and specifically comprises the following steps that 1, panax notoginseng diploid cells with the good growth condition are taken and inoculated into a WPM culture medium without phosphorus, and the WPM culture medium is inoculated into a Pseudomonas aeruginosa cell culture medium; carrying out dark culture for 5-15 days under the condition of 25 DEG C; (2) putting the pseudo-ginseng callus cultured in the step (1) into a mortar, adding liquid nitrogen, grinding, and extracting dencichine; and (3) making a dencichine standard curve, and determining the dencichine content through an HPLC method. The content of the dencichine in the panax notoginseng cells is increased through phosphorus deficiency stress, and the method is easy and convenient to operate and has important guiding significance on subsequent large-scale production of the dencichine.

The invention discloses an arthrobacterium NJAU-OF121, the classification name of the arthrobacterium NJAU-OF121 is Arthrobacter nitrophenolicus, and the preservation number of the arthrobacterium NJAU-OF121 is CGMCC (China General Microbiological Culture Collection Center) NO. 34968. The invention also discloses an acid-aluminum-resistant growth-promoting charcoal-based microbial agent, and discloses a preparation method and application of the acid-aluminum-resistant growth-promoting charcoal-based microbial agent. The arthrobacter NJAU-OF121 disclosed by the invention can grow under an acidic condition (the pH value is 4.5), and has IAA (Indoleacetic Acid) production, phosphorus solubilization and aluminum toxicity resistance under the acidic condition (the pH value is 4.5), so that a new strain resource is provided for improving the productivity of acidic soil. The survival number of functional bacteria in the acid-aluminum-resistant growth-promoting biochar-based microbial agent is remarkably increased, and the acid-aluminum-resistant growth-promoting biochar-based microbial agent has more excellent effects of promoting growth of rape (including relieving rape acid aluminum toxicity and phosphorus deficiency) and increasing yield (rape seed yield), and provides effective guarantee for high-yield cultivation of rape in acid soil.

The present application belongs to the field of microbial technology. More specifically, it relates to an acid-tolerant phosphorus-solubilizing bacterium and its application. The present application provides Trinickia diaoshuihuensis A PSM strain and a microbial inoculum formed by the PSM strain in combination with biochar. Pot experiment shows that application of the microbial inoculum of the present application can significantly increase the pH value of the soil in the counter-acid field, relieve acid damage, increase the content of soil organic carbon and total nitrogen, relieve the problem of phosphorus deficiency in the soil, improve the rhizosphere environment of the plant and significantly promote the growth of the plant. The microbial inoculum of the present application not only overcomes the bottleneck of difficult colonization and weak function of the existing microbial inoculum in the counter-acid field, but also realizes the resource utilization of rice straw, provides a green solution for the ecological restoration in the acid sulphate soil distribution area, and has good application prospect and value.

The invention relates to the field of river pollution treatment, and discloses a composite algae preparation for removing nitrogen and phosphorus pollutants and a preparation method thereof, and the composite algae preparation is prepared by compounding phosphorus-accumulating algae species, nitrogen-fixing algae species and phosphate-solubilizing bacteria which are subjected to function screening according to a specific cell number proportion. The key step of the preparation method is as follows: co-culturing the screened three microorganisms under specific pH and environmental conditions to form phycomycete symbionts with complementary functions; and then carrying out phosphorus deficiency hunger stress treatment on the obtained symbiont to induce and strengthen the excessive phosphorus uptake physiological activity of the phosphorus-accumulating algae species. By constructing a microbial system with a synergistic effect and combining a key physiological stress means, the obtained preparation can efficiently and synchronously remove nitrogen and phosphorus pollutants in a water body. The preparation prepared by the method has the advantages that the phosphorus removal rate and capability are obviously improved, the preparation process is clear, the product performance is stable, and the preparation can be prepared into wet paste or powder which is easy to store and use.

The present application relates to a kind of soil phosphorus solubilizing bacteria and carbon-based assembly material and its application in promoting rice growth, belong to the field of biotechnology, soil phosphorus solubilizing bacteria is high-efficiency phosphorus solubilizing strain: Serratia marcescens (Serratia marcescens) serratia marcescens ) NJAU56, on November 25, 2024, is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCC No.32793;The strain is superior to the currently widely used phosphorus solubilizing bacteria ATCC14581 in phosphorus solubilizing capacity;The strain and the carbon-based assembly material prepared from biochar are used in the typical double-cropping rice planting area in the middle reaches of Yangtze River with high acidity, effective phosphorus deficiency and weak fertilizer retention capacity, compared with single addition of phosphorus solubilizing bacteria or biochar, can significantly improve the strain phosphorus solubilizing efficiency and survival stability, and then improve soil phosphorus availability, soil phosphorus activation efficiency and rice phosphorus absorption, solve the problem that the existing phosphorus solubilizing strain has limited phosphorus solubilizing effect under acidic conditions.

The invention discloses application of an acid phosphatase OsPAP23 gene in efficient rice phosphorus breeding, the acid phosphatase OsPAP23 is found to have organophosphorus hydrolase activity and participate in rice phosphorus absorption and utilization regulation for the first time, expression of the acid phosphatase OsPAP23 gene is increased in a plant body, the acid phosphatase activity of rice is improved, and the yield of rice phosphorus is increased. Further, the utilization efficiency of phosphorus in the body and the utilization efficiency of organic phosphorus are improved. According to the invention, research on phosphorus absorption and transport regulation and control mechanisms is enriched, a reference mechanism is provided for corresponding phosphorus deficiency stress of rice, and the created PAP23 overexpression strain is expected to show growth and yield advantages in fields with organic fertilizers.