The invention discloses a
microorganism self-induced expression
system without a resistance
gene marker and a construction method thereof, and belongs to the technical field of
genetic engineering, the
microorganism self-induced expression
system comprises plasmids carrying a
quorum sensing system, plasmids carrying an auxotrophication system and a
chassis microorganism, the plasmids carrying the
quorum sensing system comprise LuxI genes, LuxR genes, a
promoter PL, a
promoter PLux and pYYDT vectors, and the
chassis microorganism comprises a pYYDT vector, a pYYDT vector, a pYYDT vector, a pYYDT vector, a pYYDT vector and a pYYDT vector. A
plasmid carrying an auxotroph system comprises a
nucleic acid sequence for coding
diaminopimelic acid (dap), a PCN
promoter and a pYYDT vector, a method and a tool for
synthetic biology are utilized to carry out genetic modification on
chassis microorganisms, the microorganisms are successfully subjected to genetic modification under the condition that the microorganisms do not depend on
antibiotics and inducers, and the yield of the microorganisms is improved.
Bacteria growth is carried out in the initial stage of microbial culture, when the
bacterial population density reaches a certain degree, function expression is automatically switched, dynamic allocation of resources in
bacteria is achieved, green and environment-friendly effects are truly achieved, and the application cost is reduced.