402 results about "Kiwi fruit" patented technology

The invention belongs to the field of gene engineering, and relates to a kiwi fruit AcERF56 gene and application thereof in drought resistance and growth improvement. The nucleotide sequence of the AcERF56 gene of the kiwi fruit disclosed by the invention is as shown in SEQ ID NO: 1; the amino acid sequence of the protein coded by the AcERF56 gene is as shown in SEQ ID NO: 2. The drought resistance function of the gene is reported in the kiwi fruit for the first time, the photosynthetic performance, overground and underground biomass and active oxygen scavenging capacity of the kiwi fruit under the water stress condition can be remarkably improved by overexpressing the gene, the drought resistance of the kiwi fruit is improved, and a research basis is provided for cultivation of drought-resistant and high-yield plants.

The invention discloses a shed frame type orchard kiwi fruit picking device and a picking method. The device comprises a walking mechanism; the picking mechanism is installed on the frame of the walking mechanism through a lifting mechanism and comprises a support, a driving component, a transmission shaft, picking discs and shifting fingers, the support is installed at the top end of the lifting mechanism, the driving component is installed on the support and connected with the transmission shaft, the picking discs are installed on the transmission shaft in a staggered mode, and an included angle is formed between every two adjacent picking discs; the gaps between the adjacent picking discs are periodically expanded and contracted; the shifting fingers are connected with the driving part, are arranged corresponding to the picking disc and are mounted on the bracket through a supporting shaft; the detection mechanism is used for detecting the density and height distribution of the fruits to be picked in real time to obtain detection information; and the controller is connected with the walking mechanism, the picking mechanism and the detection mechanism, dynamically plans an optimal picking path and an optimal picking sequence according to the detection information, and adjusts picking operation parameters and picking postures in real time. The invention further provides a shed frame type orchard kiwi fruit picking method.

The invention belongs to the technical field of microbial agents, and particularly relates to a preservative and fresh-keeping microbial composition as well as a microbial agent and application thereof. The microbial composition disclosed by the invention comprises bacillus subtilis LS and bacillus velezensis LX, and in the microbial composition, the concentration ratio of the bacillus subtilis LS to the bacillus velezensis LX is (0.1-1): (0.1-1); the preservation number of the bacillus subtilis LS is CGMCC (China General Microbiological Culture Collection Center) NO.30953; the preservation number of the bacillus velezensis LX is CGMCC (China General Microbiological Culture Collection Center) NO. 30954. In the microbial composition provided by the invention, the bacillus subtilis LS and the bacillus velezensis LX can generate a synergistic effect and can be used for preventing and treating the soft rot of the kiwi fruits, and a theoretical basis and technical guidance are provided for biological prevention and treatment and storage and fresh-keeping of the soft rot of the kiwi fruits.

The invention discloses bacillus tequilensis P61 and application thereof in prevention and treatment of kiwi fruit soft rot, and belongs to the field of biological prevention and treatment. The bacillus tequilensis P61 provided by the invention is preserved in the China Center for Type Culture Collection on September 5, 2024, and the preservation number of the bacillus tequilensis P61 is CCTCC (China Center for Type Culture Collection) NO: M 20241916. According to the invention, the lipopeptide antibacterial substance is prepared according to the bacillus tequilensis P61. Experimental results show that the bacillus tequilensis P61 and lipopeptide antibacterial substances have an outstanding prevention and treatment effect on the soft rot of the kiwi fruits, have a remarkable bacteriostasis effect on pathogenic bacteria of the soft rot of the kiwi fruits such as botryosphaeria dothidea, alternaria alternata and phomopsis, remarkably reduce the damage rate of the kiwi fruits, are green and safe, are harmless to people and livestock, and are worthy of popularization and application. No pollution is caused to the environment. The invention provides a new biocontrol strain for biological prevention and control of kiwi fruit diseases, and has a wide application prospect.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention discloses a special organic fertilizer for kiwi fruits and a preparation method thereof. The organic fertilizer comprises the following raw materials in parts by weight: 50-70 parts of livestock and poultry manure, 30-50 parts of kiwi fruit branch and leaf residues, 10-20 parts of soybean meal, 5-10 parts of bone meal, 3-8 parts of humic acid and 1-3 parts of a microbial agent. The microbial agent comprises bacillus subtilis, bacillus mucilaginosus, saccharomycetes and lactic acid bacteria in a ratio of 1: 1: 1: 1. The raw materials are pretreated and then subjected to a mixed fermentation process to prepare the finished product. The organic fertilizer is comprehensive in nutrition, can meet the nutritional requirements of kiwi fruit growth, improves the soil environment, improves the fruit quality and yield, and has remarkable economic benefits and environmental benefits.

The invention discloses a preparation method of a quinoa-fruit compound beverage, and belongs to the technical field of beverage processing. Comprising the following steps: S1, raw material pretreatment (in the quinoa pretreatment, high-viscosity enzymolysis modified quinoa pulp is prepared through double-enzyme synergistic hydrolysis of acid protease and natural kiwi fruit protease in kiwi fruit juice in combination with gradient heating and grinding; the preparation method comprises the following steps: pretreating raspberries, namely preparing suspended raspberry seed pulp by freeze-drying, superfine grinding and coating with enzymolysis modified quinoa pulp, and meanwhile, adding tea polyphenol, glycerol and a compound buffer agent to prepare color-protecting anti-crosslinking raspberry juice), S2, blending and homogenizing, and S3, sterilizing and filling. Through three stages of raw material pretreatment, blending and homogenization, and sterilization and filling, the nutritional conflict problems mentioned in the background technology, such as quinoa protein acid denaturation and aggregation, quinoa PPO-mediated raspberry anthocyanin oxidation browning, raspberry seed retention and suspension stability, and taste adaptation, are solved.

The present invention discloses an irrigation and fertilization device and a fertilization method for kiwifruit planting, belonging to the technical field of irrigation devices. The irrigation and fertilization device and the fertilization method for kiwifruit planting include a storage bracket. One side of the upper end of the storage bracket is fixedly installed with a reservoir, and the other side of the upper end of the storage bracket is fixedly installed with a fertilizer pool. By setting the structure of the vertical rod, the position of the spraying outer shell can be installed and fixed according to the spraying requirements of kiwifruit in different periods, so that the appropriate spraying method can be changed to fertilize and supply water to the kiwifruit fruit trees specifically, reducing the manual workload. By setting the structure of the spraying outer shell, the whole irrigation device does not need to be shut down, and the spraying outer shell can be quickly disassembled and adjusted, effectively avoiding the problems that the traditional irrigation device is easy to be blocked and damaged and needs to shut down the whole irrigation device for maintenance.

The invention provides a hyperspectrum-based kiwi fruit disease identification method, device and equipment and a medium. The hyperspectrum-based kiwi fruit disease identification method comprises the following steps: acquiring and correcting a hyperspectral image of a to-be-detected kiwi fruit sample; performing region-of-interest segmentation and spectral data extraction on the corrected hyperspectral image; preprocessing the extracted spectral data; screening characteristic wavelengths of the preprocessed spectral data through a genetic algorithm; and completing kiwi fruit disease identification on the screened characteristic wavelengths through a constructed disease identification model. The method has the advantages of simplicity, wide range, no damage and the like, the occurrence degree of diseases can be accurately found as early as possible, corresponding prevention and control measures can be taken, the loss of fruit farmers is effectively reduced, and economic benefits are increased.

The invention discloses application of a natural inducer in fruit preservation, and particularly relates to the technical field of fruit preservation. According to the application of the natural inducer in fruit preservation, the natural inducer is used as an active ingredient to inhibit growth of botrytis cinerea on fruits and induce immune systems of the fruits to enhance active oxygen metabolism; the natural inducer is theanine; the fruits are kiwi fruits. When the natural inducer provided by the invention is applied to fruit preservation, a relatively good preservation effect can be exerted on kiwi fruits.

The invention discloses an algin composite membrane which is prepared by the following steps: adding 0.8-1.2 g of algin, 0.8-1.2 g of hydroxypropyl methyl cellulose, 0.8-1.2 mL of glycerol, 0.4-0.6 g of titanium dioxide and 0.8-1.2 g of polylysine into water, uniformly mixing, fixing the volume, putting into a container, drying, immersing into a CaCl2 solution for crosslinking treatment, and drying, thereby obtaining the algin composite membrane. The algin composite film can be used as or used for preparing a food preservative film. The algin composite film disclosed by the invention realizes good balance between strength and ductility, has good antioxidant capacity and remarkable antibacterial effect, and can effectively enhance the antioxidant defense capacity of the cape gooseberries, reduce oxidative damage, prolong the storage period and improve the fresh-keeping effect of the cape gooseberries. According to the method, the economic value of the sargassum is favorably improved, and powerful support is provided for sustainable development of the food industry.

The invention discloses lactococcus lactis M6 and application thereof, and belongs to the technical field of microorganisms. The lactococcus lactis M6 provided by the invention is obtained by separating and purifying a kiwi fruit sample picked in Sichuan of China, has multiple functions of high yield of gamma-aminobutyric acid (GABA), high yield of butyric acid, oxidation resistance, antibacterial performance and the like, and has great industrial application value.

The invention provides a probiotic composition for inhibiting helicobacter pylori as well as a probiotic agent and application thereof, and belongs to the technical field of probiotic functional products. The probiotic composition is prepared from the following components in parts by weight: 0.05 to 2 parts of a pediococcus pentosaceus strain A21358, 0.01 to 1.5 parts of a plant lactobacillus strain A21136, 0.05 to 1.5 parts of a lactobacillus rhamnosus strain A21149 and 0.03 to 1.2 parts of a lactobacillus reuteri strain A21325. The probiotic agent comprises the probiotic composition, resistant dextrin, xylooligosaccharide, erythritol, inulin, fructo-oligosaccharide, hericium erinaceus powder, fucoidin and at least one fruit powder selected from grape powder, passion fruit powder and kiwi fruit powder, wherein the specific weight parts of the resistant dextrin, the xylooligosaccharide, the erythritol, the inulin, the fructo-oligosaccharide, the hericium erinaceus powder, the fucoidin and the at least one fruit powder are selected from grape powder, passion fruit powder and kiwi fruit powder. The probiotic agent has the effect of inhibiting helicobacter pylori, and a new thought is provided for clinical related auxiliary treatment of helicobacter pylori infection.

The invention discloses burkholderia gladioli BG-GF5 as well as a composition and application thereof, the burkholderia gladioli BG-GF5 is preserved in the China Center for Type Culture Collection with the preservation number of CCTCC NO: M20242573, and the preservation date is November 25, 2024. The application of the BG-GF5 is mainly reflected in the inhibition effect of the BG-GF5 on pathogenic bacteria and the potential of the BG-GF5 in crop disease control. The strain or the fermentation product and the metabolite thereof can be prepared into a composition for inhibiting pathogenic bacteria and crop diseases caused by the pathogenic bacteria. Particularly, the application to crops such as bee sugar plums, chilies and kiwi fruits shows a wide application prospect in the field of agricultural biological control.

The invention discloses a key gene AaMYB114 closely linked with the color character of an actinidia arguta fruit and application of the key gene AaMYB114. The AaMYB114 gene disclosed by the invention is positioned at 5237694-5239966bp of a chromosome 9 of an actinidia arguta genome, and the full length of the AaMYB114 gene is 2273bp. According to the application, all-red type and all-green type actinidia arguta are used as materials for transcriptome sequencing, and the gene AaMYB114 related to the formation of the peel color of the actinidia arguta is explored. Instantaneous conversion of the actinidia arguta fruit proves that the overexpression of the AaMYB114 increases the anthocyanin content in the actinidia arguta peel. Yeast one-hybridization and dual-luciferase report tests prove that the AaMYB114 not only interacts with a promoter of AaGST, activates the expression of the promoter and regulates anthocyanin transport of peel tissues of actinidia arguta, but also is combined with promoters of anthocyanin synthesis structural genes AaPAL, AaCHI and AaF3H and activates the expression of the promoters to promote anthocyanin synthesis. Therefore, the AaMYB114 gene plays a dual regulation and control role in the formation of peel color and luster, and can be used for cultivating red-peel actinidia arguta.

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

The invention discloses a traditional Chinese medicine compound extracted plant beverage which comprises the following raw materials in parts by weight: 20-30 parts of a traditional Chinese medicine compound extracting solution, 40-60 parts of composite concentrated fruit juice, 2-4 parts of a preservative, 0.2-0.5 part of a stabilizer and the balance of water, totaling 500 parts, the solid content of the traditional Chinese medicine compound extracting solution is 25-35%, and the traditional Chinese medicine compound extracting solution is prepared by extracting, filtering and concentrating radix astragali, fructus lycii, rhizoma dioscoreae, poria cocos, fructus crataegi and flos chrysanthemi; the solid content of the composite concentrated fruit juice is 30-40%, the composite concentrated fruit juice is prepared by pulping, filtering and concentrating blueberries, lemons, kiwi fruits and grapefruits, and the composite concentrated fruit juice contains a natural antioxidant. In addition, the invention further discloses a preparation method of the traditional Chinese medicine compound extracted plant beverage, and the traditional Chinese medicine compound extracted plant beverage not only is good in taste, but also has a remarkable fatigue relieving function and has a wide market application prospect.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an AcAAT1 gene and application thereof in regulation and control of formation of fruit ester aroma. The invention provides a kiwi fruit AcAAT1 gene related to ester aroma formation and a protein coded by the kiwi fruit AcAAT1 gene, and constructs a corresponding overexpression vector and a transient silence vector which are transferred into kiwi fruits. Results show that the content of ester aroma in kiwi fruits can be increased by overexpressing the AcAAT1 gene, and the content of ester aroma in kiwi fruits can be reduced by silencing the AcAAT1 gene. The actinidia chinensis AcAAT1 gene disclosed by the invention can be used for promoting the formation of ester aroma. The method disclosed by the invention has very important application value in the direction of accurately creating new germplasm of the kiwi fruit with strong fragrance through a genetic improvement mode.

The utility model discloses watering equipment for kiwi fruit planting, a water delivery mechanism comprises a water delivery pipe, the two ends of the water delivery pipe are fixedly connected with threaded heads, the outer wall of the water delivery pipe is fixedly connected with sealing rings located on the outer sides of the threaded heads, the tops of the sealing rings are fixedly connected with limiting rails, and the limiting rails are fixedly connected with the water delivery pipe. A connecting pipe is movably connected to the position, close to the threaded head, of the water conveying pipe, threaded grooves are formed in the two ends of the inner wall of the connecting pipe, the inner sides of the threaded grooves are in threaded connection with the outer wall of the threaded head, the surface of the connecting pipe makes movable contact with the sealing ring, and a limiting sleeve is fixedly connected to the upper end of the inner wall of the connecting pipe; a lifting pipe is slidably connected to the inner wall of the limiting sleeve, an extrusion shaft is fixedly connected to the middle of the top of the lifting pipe, a connecting pipe is fixed to the end of the water conveying pipe, the lifting pipe is arranged in the connecting pipe, the lifting pipe moves downwards, water flow in the water conveying pipe can enter the water guide opening, and the water flow can be output from the lower portion of the water guide opening to wet nearby soil.

The invention provides a mechanism for regulating and controlling AsA content of kiwi fruits by AcMYB73 and AcMYB108 transcription factors and application of the mechanism. According to the application disclosed by the invention, after GABA is used for treating kiwi fruits, transcriptome sequencing is carried out, and AcMYB73 and AcMYB108 transcription factors which are most likely to regulate and control ascorbic acid-related metabolic genes are screened from numerous transcription factors with most obvious abundance change; experiments such as LUC / REN dual luciferase, yeast single impurity, gel migration and the like prove that both the AcMYB73 and the AcMYB108 can positively regulate and control the ascorbic acid synthesis gene and the circulating gene of the kiwi fruit and reversely regulate and control the ascorbic acid degradation gene of the kiwi fruit at the same time. The invention also successfully constructs overexpression vectors and interference vectors of AcMYB73 and AcMYB108, and successfully realizes regulation and control of the content of ascorbic acid in kiwi fruits.

The utility model discloses a grinding device for kiwi fruit powder processing, which comprises a support frame and a charging barrel arranged in the support frame, a crushing component is arranged on one side of the upper end of the inner wall of the charging barrel, a feed hopper is arranged at the upper end of the crushing component, a driving motor is mounted at the upper end of the outer wall of the charging barrel, and a first belt wheel is arranged on the outer wall of a power output shaft of the driving motor. A worm is rotationally connected to one side of the outer wall of the charging barrel, a second belt wheel is arranged at the upper end of the worm, a guide hopper is arranged at the upper end of the inner wall of the charging barrel, the lower end of the guide hopper communicates with the crushing assembly, and a control panel is arranged on one side of the upper end of the supporting frame; the kiwi fruit grinding machine is easy to operate, grinding work of kiwi fruits can be completed without frequent disassembly and assembly, and during grinding, continuous feeding can be achieved, the grinding efficiency can be improved, and meanwhile the grinding effect on the kiwi fruits is not affected.

The waterlogging-resistant irrigation device for kiwi fruit planting comprises a pipe body, a movable groove is formed in the pipe body, a plurality of first dripping holes and a plurality of second dripping holes are formed in the pipe body, a movable plate is arranged in the movable groove in a sliding mode, one side of the movable plate is fixedly connected with a first cleaning plate, and the other side of the movable plate is fixedly connected with a second cleaning plate. The other side of the movable plate is fixedly connected with a second cleaning plate, and a moving groove is formed in the pipe body. And the multiple first dripping holes are distributed at equal intervals in an array mode, the multiple second dripping holes are aligned with the first dripping holes, and the first cleaning plate and the second cleaning plate are both matched with the moving groove. According to the waterlogging-resistant irrigation device for kiwi fruit planting, the problem that the drip holes are blocked by sediments is solved, so that a planted kiwi fruit tree is conveniently watered and irrigated by a pipeline through the drip holes, the irrigation operation is ensured not to be influenced, and the waterlogging-resistant irrigation device is helpful for the growth quality of kiwi fruits.

The invention provides an antibacterial peptide, application of the antibacterial peptide and an antibacterial drug and a preservative prepared from the antibacterial peptide, and belongs to the technical field of biology. The antibacterial peptide is an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3 or an amino acid sequence which has more than 95% of homology with the amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 2 or SEQ ID NO: 3. The three antibacterial peptides have broad-spectrum antibacterial effects, have obvious inhibition effects on gram-positive bacteria, gram-negative bacteria and fungi, and can be prepared into antibacterial drugs for use. And simultaneously. The antibacterial film agent prepared from the antibacterial peptide can be used as an antibacterial preservative, and can delay proliferation of putrefying bacteria in food or medicines, so that the shelf life of the food or medicines is prolonged, for example, the antibacterial peptide can be used for preventing and treating soft rot of kiwi fruits. The antibacterial peptide has a good application prospect in the fields of food, medicine and the like.

The utility model discloses a variable frequency pressure difference airflow control device based on a kiwi fruit ripening control device, comprising a device body, wherein an air outlet bin is symmetrically fixedly connected to the top of the device body, slide grooves are symmetrically arranged inside the device body, electric push rods are symmetrically fixedly connected to one side of the slide grooves, a baffle is fixedly connected to the output end of the electric push rod, a second air outlet is symmetrically arranged on one side of the device body, an air outlet pipe is symmetrically fixedly connected to the upper end of the second air outlet, a third motor inside the gear box drives the driving wheel to rotate, the driving wheel is meshed and connected with the driven wheel, the driven wheel is connected to the fixed plate through the second connecting rod, so as to realize the rotation of the fixed plate, a second motor, a rotating shaft and fan blades are installed at the bottom of the fixed plate, so as to realize automatic control of the flow direction of the collected gas in the device body, increase the effect of uniform distribution of the gas in the ripening control device, and help to improve the performance, safety and processing efficiency of the ripening control device.

The utility model discloses an insect repellent device for kiwifruit planting, comprising an unmanned aerial vehicle (UAV) body, a medicine storage box being arranged below the UAV body, a spraying mechanism being arranged on the UAV body, the spraying mechanism comprising a supporting frame, a connecting piece, a conveying pipe, a nozzle and a water pump, the supporting frame being mounted on the UAV body, the connecting piece being provided with a plurality of groups mounted on the supporting frame, the conveying pipe being mounted on the connecting piece, the nozzle being mounted on the bottom end of the connecting piece, the water pump being mounted on one side of the connecting piece, a medicine extraction mechanism being arranged below the medicine storage box, the medicine extraction mechanism comprising a vacuum pump, a fixed pipe, a connecting pipe, a threaded pipe and a medicine extraction head, the vacuum pump being mounted on the bottom surface of the medicine storage box, so as to solve the technical problems mentioned in the background technology that the spraying of the insect repellent is uneven, errors are likely to occur when the insect repellent is added manually, and the mixing of the insect repellent and water in the medicine storage box is uneven.

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition for treating prostatitis and a preparation method thereof. The traditional Chinese medicine composition for treating prostatitis comprises the following raw materials in parts by weight: 13-20 parts of salvia miltiorrhiza, 15-20 parts of erigeron breviscapus, 10-16 parts of actinidia chinensis root, 8-15 parts of loquat flower, 5-10 parts of sambucus chinensis root, 8-12 parts of astragalus membranaceus, 2-9 parts of curcuma aromatica and 2-7 parts of liquorice. According to the traditional Chinese medicine, all the medicines are combined to achieve the effects of promoting blood circulation to remove meridian obstruction, clearing heat, eliminating dampness, inducing diuresis, relieving pain and the like, the treatment effect on prostatitis is good, and a new treatment thought and a new medicine choice are provided for prostatitis.

The invention discloses a bactericide containing organic metal salt and a preparation method thereof, the bactericide is generated by chemical reaction of N-phenyl-o-carboxybenzamide and metal inorganic salt, and the preparation method specifically comprises the following steps: adding the metal inorganic salt into water to obtain a metal inorganic salt solution; adding N-phenyl o-carboxybenzamide into the metal inorganic salt solution to obtain a mixture A; heating the mixture A to 55-90 DEG C, keeping the constant temperature for 60-150 minutes, cooling the mixture A to room temperature after the reaction is finished, and then adjusting the pH value of the mixture A to 6-10 to obtain a mixture B; and heating the mixture B to 70-90 DEG C, keeping the constant temperature for 3-5 hours, cooling the mixture B to room temperature after the reaction is finished, filtering, and drying the filtered solid to obtain the bactericide. The bactericide disclosed by the invention not only has good sterilization and control effects on apple tree canker and kiwi fruit canker, but also has a certain regulation effect on plant growth.

The utility model discloses a kiwi fruit cultivation device which comprises a device body, a cultivation box is arranged on the device body, an oxygen supply mechanism is fixed to one side of the top of the cultivation box, a carbon dioxide supply mechanism is fixed to one side of the oxygen supply mechanism, and a watering mechanism is fixed to the other side of the top of the cultivation box. A plurality of sliding grooves are fixed to the two side walls of the interior of the cultivation box, cultivation grooves are movably connected to the sliding grooves, a water collecting groove is formed in the bottom of the interior of the cultivation box, and an auxiliary detection mechanism is fixed to the inner wall of the cultivation box. Through the arrangement of the water spraying mechanism, water spraying is facilitated, excessive seeping water can be conveniently collected and recycled, resources are saved, and meanwhile it is guaranteed that the interior of the cultivation box is clean.

The invention provides an ISSR-PCR reaction system for identifying kiwi fruit varieties with different colors and a method and application of the ISSR-PCR reaction system, and belongs to the technical field of molecular markers. The ISSR-PCR reaction system for identifying the kiwi fruit varieties with different colors is as follows: the DNA template is 70ng / mu L, the primer is 1.0 mu mol / L, the Mg < 2 + > is 2mM, the mix is 10mu L, and the remaining ddH2O is prepared into 20 mu L of the system. The amplification procedure is as follows: the pre-denaturation temperature is 94 DEG C, and the time is 5 minutes; the denaturation temperature is 94 DEG C for 30 seconds; the annealing temperature is set according to different primer conditions, and the time is 50s; the extension temperature is 72 DEG C, and the extension time is 1 min; circulation is performed for 35 times; and then extending at 72 DEG C for 7 minutes, and storing at 10 DEG C. The system is used for amplifying 42 kiwi fruit germplasm resources with different colors, the amplification result shows that the established ISSR-PCR reaction system is good in repeatability and high in stability, and the established ISSR molecular fingerprint spectrum can be used for variety identification.

The utility model relates to the technical field of agricultural product processing, in particular to drying equipment for processing preserved kiwi fruits. The drying machine comprises a drying machine body, a turnover mechanism is arranged at the center in the drying machine body, a rotating mechanism is arranged on the upper portion in the drying machine body, the turnover mechanism comprises a motor fixedly matched with one side of the drying machine body, and the output end of the motor penetrates through the drying machine body and is fixedly provided with a connecting shaft; transmission shafts in running fit with one side of the inner wall of the drying machine body are arranged at the two ends of the connecting shaft through multiple sets of belt wheels and conveying belts in a transmission mode, and a containing basket is fixedly arranged between every two transmission shafts. Kiwi fruit blocks in the turnover mechanism are turned over through operation of the turnover mechanism, namely, the two faces of each kiwi fruit block are turned over multiple times and make full contact with hot air in the drying machine body, the drying efficiency is improved, the hot air in the drying machine body is blown through the rotating mechanism, the flowing speed of the hot air is increased, and the drying efficiency is improved. And the drying efficiency is further improved.