107 results about "Brassicaceae" patented technology

The invention discloses application of oilseed rape chitinase EP3 and a coding gene thereof in improving clubroot resistance of cruciferous plants, and belongs to the technical field of molecular biology. The invention discloses the application of the rape chitinase EP3 in improving the clubroot resistance of plants for the first time. The amino acid sequence of the rape chitinase EP3 is as shown in SEQ ID NO.2; the nucleotide sequence of the coding gene EP3 is shown as SEQ ID NO. 1. Experimental results show that by preparing agrobacterium containing an EP3 gene overexpression vector and using a treating fluid containing the agrobacterium to perform seed soaking and root irrigation treatment, the EP3 gene is overexpressed in a rape plant, and the clubroot resistance of the rape can be remarkably improved. The invention provides a new gene resource and a safe, efficient, simple, convenient and universal prevention and treatment method for preventing and treating clubroot of cruciferous plants, and has remarkable agricultural application value and wide application prospect.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The invention discloses a straw returning method based on corn and covered crop cultivation, which comprises the following steps: grading and crushing corn straws according to soil types, carrying out zoning treatment, combining a leguminous-gramineae-cruciferae ternary mixed sowing covered crop system, and matching a decomposition microbial inoculum and controlled release nitrogen fertilizer synergistic regulation technology. The efficient decomposition of the straws and the improvement of the soil quality are realized. The method can increase soil organic matters by 0.25%, increase corn yield by 12.7% and reduce nitrogen fertilizer loss by more than 30%, and is suitable for sustainable production in dry farming areas.

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention provides a composite material of plant cell membrane vesicles and cerium-based nano-enzyme. The composite material structurally comprises the plant cell membrane vesicles and the cerium-based nano-enzyme attached to the surfaces of the plant cell membrane vesicles, cerium ions of the cerium-based nano-enzyme and carboxyl groups on the surfaces of the plant cell membrane vesicles form coordination bonds; the plant cell membrane vesicles are from cruciferous plants, and carboxyl groups on the surfaces of the plant cell membrane vesicles comprise free carboxyl groups connected with the surfaces of the plant cell membrane vesicles through-NH-CO-PEGn-structures; the mass ratio of the cerium ions to the plant cell membrane protein is (0.1-0.5): 1. The invention also provides a method for preparing the composite material and application of the composite material in sepsis treatment. The composite material can specifically adsorb endotoxin and efficiently degrade the endotoxin, can be used for preventing / rescuing endotoxin-induced cell inflammation, and can also effectively relieve organ failure of sepsis model mice.

Disclosed is a combination in which, when at least one target gene present at the S locus is amplified by PCR, a tail sequence that does not hybridize to the nucleic acid sequence of the target gene and does not affect the amplification reaction is added to the 5' side of at least one primer so that the S haplotype can be identified based on the size difference of the amplified DNA fragment, and a size difference occurs in the amplified DNA fragment. The haplotype can be identified through a simple method, such as electrophoresis, due to a size difference occurring in the amplified DNA fragment. The present invention makes it possible to provide a method for easily identifying the S haplotype that controls self-incompatibility in plants.

The present application relates to the technical field of vegetable cultivation, and particularly relates to a method for creating high anthocyanin purple cruciferous vegetables by symbiosis with purple tea. The specific technical scheme is as follows: the method for creating high anthocyanin purple cruciferous vegetables by symbiosis with purple tea, a green cruciferous vegetable conventional variety and a purple tea variety with high anthocyanin content are selected for symbiotic cultivation, and after flowering and seed saving, multiple purification and verification are performed, and finally a high anthocyanin purple cruciferous vegetable pure hybrid material with consistent genetic characteristics is obtained. The present application can obtain a high anthocyanin purple cruciferous vegetable pure hybrid material by symbiotic cultivation of a green cruciferous vegetable conventional variety and a purple tea variety through a conventional breeding method, and after multiple seed saving and verification, the commercialization degree and nutritional value of the high anthocyanin purple cruciferous vegetable are significantly improved.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP31 and application of the Chinese cabbage clubroot-resistant gene BrRLP31. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP31 is as shown in SEQ ID No. 1. Functional verification results show that after the expression of the endogenous BrRLP31 of the Chinese cabbage is inhibited by utilizing a VIGS technology, the resistance of the plant to clubroot is obviously reduced, and the disease index and the root swelling severity of the plant are obviously higher than those of a control material. On the other hand, the transgenic flowering cabbage plant over-expressed by the BrRLP31 is constructed by an agrobacterium-mediated method, and disease resistance identification proves that the heterologous over-expression of the BrRLP31 obviously improves the resistance of the plant to plasmodiophora brassicae. The results show that the BrRLP31 gene plays a key positive regulation role in the clubroot resistance process of the Chinese cabbage, the disease resistance of cruciferae crops and other horticultural plants is improved by utilizing the gene and the homologous sequence thereof, and the gene and the homologous sequence thereof have important application value.

This utility model relates to the field of windproof isolation covers, specifically disclosing a windproof isolation cover for cruciferous hybrid breeding. It includes a first positioning rod, which is a round rod structure. Two symmetrically arranged first positioning rods constitute a first support unit. In this windproof isolation cover for cruciferous hybrid breeding, the device is supported by a first support unit and a second support unit. The two first positioning rods within the first support unit are connected by a first connecting rod. The second connecting rod is fixedly connected to the upper surface of the second positioning rod within the second support unit, further strengthening the support structure and enabling it to better resist wind. Simultaneously, fixing nails installed at the lower ends of each first and second positioning rod can be inserted into the ground for fixation. The equidistantly arranged fixing rods in a ring on the partition plate can rotate, further securing the device and preventing it from swaying or shifting in the wind, thus improving the device's wind resistance.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

The invention belongs to the technical field of plant genetic engineering, and relates to an application of a Xanthomonas oryzae hexose phosphate mutase encoding gene in the aspect of adjusting the susceptibility of cruciferous plant arabidopsis thaliana. According to the present invention, the recombinant plasmid is constructed, the Xanthomonas oryzae pv.oryzae (Xoo) PXO99A hexose phosphate mutase coding gene PXO03174 is adopted as the target gene to be introduced into the cruciferous plant arabidopsis thaliana, such that the condition that the plant with the overexpression of the gene is sensitive to the infection of the pathogenic bacterium Pseudomonas syringae pv.tomato (Pst) DC3000 is found, and the significantly enhanced disease infection characteristic is represented; according to the present invention, the discovery can be used for developing the sensitive bacterial disease detection method so as to improve the accuracy of bacterial disease detection; the method can also be used for identifying toxic genes of plant pathogenic bacteria such as pathogenic bacteria sensitive plants and pseudomonas syringae and researching the mechanism of the infected plants, and can also be used for researching the disease resistance of the plants to the pseudomonas syringae and developing antibacterial agents.

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

The application belongs to the technical field of genetic engineering, and particularly relates to application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases. The application provides application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases, and the amino acid sequence of the AtPEN2 protein is shown as SEQ ID NO. 1. The application takes Arabidopsis thaliana as a model plant to verify the prevention and treatment effect of overexpression of AtPEN2 protein on clubroot, and the results show that the AtPEN2 protein overexpression plant can improve the resistance to clubroot, indicating that the coding gene AtPEN2 of the AtPEN2 protein can be applied to molecular breeding of clubroot, and provides a new gene resource for molecular breeding of cruciferous crops.

The present application provides a kind of Cardamine macrophylla Willd. Uniform polysaccharide, it is derived from the whole grass of Brassicaceae Pteryxia plant Cardamine macrophylla Willd., and the preparation method and purpose of the Cardamine macrophylla Willd. Uniform polysaccharide are provided.The present application realizes the efficient extraction of Cardamine macrophylla Willd. Uniform polysaccharide by single factor multi-level experiment combined with Box-Behnken central combination design-response surface method optimization of high pressure auxiliary low eutectic solvent extraction Cardamine macrophylla Willd. Uniform polysaccharide optimum extraction process parameter.The Cardamine macrophylla Willd. Uniform polysaccharide prepared in the present application is rich in galacturonic acid glycan and I type rhamnose galacturonic acid glycan acidic polysaccharide, has good antioxidant activity and immune regulation activity.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The utility model discloses germination accelerating equipment for cruciferous vegetable seeds, and relates to the technical field of vegetable seed germination accelerating, the germination accelerating equipment comprises a hollow column, electric heating plates are fixed on two sides of the inner wall of the hollow column, seed mixed soil is put into a heat conduction culture dish with holes, the inner wall of the heat conduction culture dish with holes slides on the outer wall of a sliding column, and the electric heating plates are fixed on the inner wall of the hollow column. A heat conduction culture dish with holes is placed in a hollow column, and the outer wall of an insertion block and the inner wall of the hollow column are clamped and mounted, so that the heat conduction culture dish with holes is stably mounted on the inner wall of the hollow column, and the situation that the seeds absorb water unevenly due to the fact that a worker manually adds a water source is avoided; the bottom end of the hollow column and the top end of the groove column are fixedly installed until the bottom end of the pore plate makes contact with the top end of the supporting block, a fan is controlled to blow air into the hollow column through the pore plate, cold air is dried and heated through an electric heating plate, and hot air makes contact with the heat conduction culture dishes with the holes.

The invention provides a method for preventing and controlling plant aphids, which comprises the following specific steps: selecting plant germinated seeds with roots and root length of 2-6 cm, and in the growth process of the plant germinated seeds, applying light for 8 hours and photoperiod treatment in a dark cycle mode for 16 hours; in the plant germinated seeds, the seed sources comprise beans and cruciferous vegetables; meanwhile, in the growth process of the germinated seeds of the plants, a boron-containing nutrient solution is prepared from the following components: 4mM of Ca (NO3) 2.4 H2O, 1mM of KH2PO4, 1mM of KCl, 2mM of MgSO4. 7H2O, 0.5 mM of K2SO4, 50mu M of FeSO4. 7H2O, 50mu M of EDTA.Na2, 10mu M of MnCl2, 5mu M of ZnSO4. 7H2O, 0.5 mu M of CuSO4. 5H2O, 1mu M of (NH4) 6Mo7O24. 4H2O, 35-70 mu M of H3BO3 and 0.5 mu M of CoCl2; nutrient elements are applied according to the following steps that (1) a first boron-containing nutrient solution is adopted in 1-5 days, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; and (2) after 6-15 days, adopting a second boron-containing nutrient solution, wherein the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The present invention discloses the Brassica rapa var. parachinensis BrAL3 gene and its application in promoting the bolting and flowering processes of cruciferous plants, belonging to the field of biotechnology. Overexpression of the Brassica rapa var. parachinensis BrAL3 gene can promote the bolting and flowering processes of cruciferous plants. In the present invention, the Brassica rapa var. parachinensis BrAL3 gene is introduced into cruciferous plants by the Agrobacterium-mediated method to obtain transgenic lines with overexpression of the Brassica rapa var. parachinensis BrAL3 gene. The results show that the expression change of the Brassica rapa var. parachinensis BrAL3 gene can significantly change the bolting and flowering time of plants. Among them, the cruciferous plants can be Arabidopsis thaliana, Brassica rapa var. parachinensis or other variants of Brassica napus, as well as other cruciferous vegetables. That is, this gene can be applied to the variety breeding of early-bolting Chinese cabbage vegetables such as Brassica rapa var. parachinensis and Brassica rapa var. utilis, etc., and has good application prospects.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The present invention belongs to the field of plant genetic engineering and discloses the use of a gene for promoting the accumulation of very-long-chain fatty acids in plant seeds. The gene fragment comprises a nucleotide sequence as shown in any one of SEQ ID No. 1 to SEQ ID No. 5, or a complementary sequence thereof, or a derivative nucleotide sequence with at least 95% homology to these nucleotide sequences and the same function. The carbon chain of the very-long-chain fatty acid has greater than 18 carbon atoms, and the gene fragment is capable of increasing the proportion of very-long-chain fatty acids in phosphatidylcholine (PC) and / or phosphatidylethanolamine (PE) in plant seeds. The present invention utilizes the LPAT2 gene (nucleotide sequence, for example, as shown in SEQ ID No. 1 to SEQ ID No. 5) from a cruciferous plant to increase the percentage of very-long-chain fatty acids in phosphatidylcholine and phosphatidylethanolamine in plant seeds, overcoming technical bottlenecks encountered in the current field.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The application provides a Chinese cabbage root knot disease related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to the following technical scheme. The CDS sequence of the Chinese cabbage BrSWEET14a is shown as SEQ ID No. 1. The gene is transformed into Col-type Arabidopsis thaliana through an Agrobacterium flower dipping transformation method, and a BrSWEET14a heterologous expression Arabidopsis thaliana strain is obtained. It is found that the T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to root knot disease than the Col-type, and the heterologous expression of the Chinese cabbage BrSWEET14a leads to the decrease of the disease resistance of Arabidopsis thaliana to the root knot fungus. It can be seen that the Chinese cabbage BrSWEET14a is closely related to the root knot disease, and the gene and the homologous genes of the gene in other cruciferous plants can be applied to the breeding of cruciferous plants and other horticultural plants, and have a good application prospect.

The present invention provides formulations with specific pesticidal activity against insects of the order Homoptera, based on a characterized strain of Bacillus megaterium which produces spores, crystals, primary and secondary metabolites, bioactive substances and degradative enzymes, where the bacteria and / or its fermentation products, both intracellular and extracellular are active ingredients, with pesticidal activity both in vitro and when applied in any phenological state of the plant, both to foliage, fruit, stem, root, for various botanical groups such as Bulbs; brassicas; legumes; nightshades; cucurbits; cereals; citrus, berries, forest and leafy vegetables.

The application discloses a Higgins anthracnose pathogenic gene ChTHR1 and application thereof. The nucleotide sequence of the ChTHR1 gene is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.3. The application determines that the pathogenic gene ChTHR1 has a key role in spore production, the rate of appressorium formation, mycelium biomass, ultraviolet resistance, melanin production and melanin accumulation of Higgins anthracnose, and can be used as a drug target for drug design and screening against Higgins anthracnose. Higgins anthracnose spreads between leaf blades of Brassica parachinensis and other cruciferous vegetables in Guangdong by means of conidia, and the conidia are eliminated or inhibited to prevent the prevalence of plant anthracnose. The ChTHR1 gene is used as a target for drug development, a drug for preventing and treating anthracnose caused by Higgins anthracnose and reducing the pathogenicity of plant Higgins anthracnose is screened, and the effect of the drug is evaluated.

The invention provides application of mustard oil in preparation of a pesticide for preventing and treating cruciferous pest plutella xylostella. The invention further provides the Pickering emulsion gel containing the mustard oil, and the invention further provides the Pickering emulsion gel containing the mustard oil. According to the preparation method of the green pesticide mustard oil Pickering emulsion gel provided by the invention, the mustard oil is entrapped in the nano-carrier to form the stable Pickering emulsion gel. The compound is simple in preparation process, green and environment-friendly, high in safety and stability and relatively high in killing power to pests, has antibacterial activity as a green pesticide, is green and efficient, is easily degraded by the environment, can remarkably improve the pesticide efficiency and reduce the environmental influence, and solves the problems of high toxicity, difficulty in degradation and low utilization rate of the existing pesticide.

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.

The invention discloses a method for identifying plutella xylostella-resistant germplasm resources of cruciferous vegetable crops. The identification method comprises an identification device and an identification method, the identification device is provided with a breathable net on a cover body of a culture dish, agarose, filter paper and moisturizing cotton are combined to construct a proper feeding environment for the plutella xylostella, and a field feeding process is truly simulated under indoor conditions. On the basis of the device, an insect resistance identification method is established, three-instar larvae of plutella xylostella are inoculated and cultured regularly, and larva death number, weight change and leaf damage data are obtained; and further constructing a germplasm resource identification method for resisting the plutella xylostella of the cruciferous vegetable crops, and carrying out quantitative analysis and grade judgment on the insect resistance of the cruciferous vegetable crops by taking the larva death rate, the body weight inhibition rate and the leaf damage rate as evaluation indexes. The identification device and method cooperate with each other to form a standardized cruciferous vegetable crop insect-resistant resource identification system.

The invention discloses a probe method real-time fluorescent PCR (polymerase chain reaction) rapid detection primer and probe combination for cruciferae bacterial phytophthora blight and a detection method, and belongs to the technical field of biological detection. According to the probe method real-time fluorescent PCR rapid detection method for the cruciferae bacterial phytophthora blight, DNA of a tested strain is used as a template, and the probe method real-time fluorescent PCR rapid detection is carried out by using a primer and probe combination. The detection method has the advantages of high specificity, high accuracy, high sensitivity, rapidness, high efficiency, quantitative analysis, simple operation, good repeatability and strong applicability.