81 results about "Brassicaceae" patented technology

The invention discloses application of oilseed rape chitinase EP3 and a coding gene thereof in improving clubroot resistance of cruciferous plants, and belongs to the technical field of molecular biology. The invention discloses the application of the rape chitinase EP3 in improving the clubroot resistance of plants for the first time. The amino acid sequence of the rape chitinase EP3 is as shown in SEQ ID NO.2; the nucleotide sequence of the coding gene EP3 is shown as SEQ ID NO. 1. Experimental results show that by preparing agrobacterium containing an EP3 gene overexpression vector and using a treating fluid containing the agrobacterium to perform seed soaking and root irrigation treatment, the EP3 gene is overexpressed in a rape plant, and the clubroot resistance of the rape can be remarkably improved. The invention provides a new gene resource and a safe, efficient, simple, convenient and universal prevention and treatment method for preventing and treating clubroot of cruciferous plants, and has remarkable agricultural application value and wide application prospect.

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention provides a composite material of plant cell membrane vesicles and cerium-based nano-enzyme. The composite material structurally comprises the plant cell membrane vesicles and the cerium-based nano-enzyme attached to the surfaces of the plant cell membrane vesicles, cerium ions of the cerium-based nano-enzyme and carboxyl groups on the surfaces of the plant cell membrane vesicles form coordination bonds; the plant cell membrane vesicles are from cruciferous plants, and carboxyl groups on the surfaces of the plant cell membrane vesicles comprise free carboxyl groups connected with the surfaces of the plant cell membrane vesicles through-NH-CO-PEGn-structures; the mass ratio of the cerium ions to the plant cell membrane protein is (0.1-0.5): 1. The invention also provides a method for preparing the composite material and application of the composite material in sepsis treatment. The composite material can specifically adsorb endotoxin and efficiently degrade the endotoxin, can be used for preventing / rescuing endotoxin-induced cell inflammation, and can also effectively relieve organ failure of sepsis model mice.

Disclosed is a combination in which, when at least one target gene present at the S locus is amplified by PCR, a tail sequence that does not hybridize to the nucleic acid sequence of the target gene and does not affect the amplification reaction is added to the 5' side of at least one primer so that the S haplotype can be identified based on the size difference of the amplified DNA fragment, and a size difference occurs in the amplified DNA fragment. The haplotype can be identified through a simple method, such as electrophoresis, due to a size difference occurring in the amplified DNA fragment. The present invention makes it possible to provide a method for easily identifying the S haplotype that controls self-incompatibility in plants.

The present application relates to the technical field of vegetable cultivation, and particularly relates to a method for creating high anthocyanin purple cruciferous vegetables by symbiosis with purple tea. The specific technical scheme is as follows: the method for creating high anthocyanin purple cruciferous vegetables by symbiosis with purple tea, a green cruciferous vegetable conventional variety and a purple tea variety with high anthocyanin content are selected for symbiotic cultivation, and after flowering and seed saving, multiple purification and verification are performed, and finally a high anthocyanin purple cruciferous vegetable pure hybrid material with consistent genetic characteristics is obtained. The present application can obtain a high anthocyanin purple cruciferous vegetable pure hybrid material by symbiotic cultivation of a green cruciferous vegetable conventional variety and a purple tea variety through a conventional breeding method, and after multiple seed saving and verification, the commercialization degree and nutritional value of the high anthocyanin purple cruciferous vegetable are significantly improved.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP31 and application of the Chinese cabbage clubroot-resistant gene BrRLP31. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP31 is as shown in SEQ ID No. 1. Functional verification results show that after the expression of the endogenous BrRLP31 of the Chinese cabbage is inhibited by utilizing a VIGS technology, the resistance of the plant to clubroot is obviously reduced, and the disease index and the root swelling severity of the plant are obviously higher than those of a control material. On the other hand, the transgenic flowering cabbage plant over-expressed by the BrRLP31 is constructed by an agrobacterium-mediated method, and disease resistance identification proves that the heterologous over-expression of the BrRLP31 obviously improves the resistance of the plant to plasmodiophora brassicae. The results show that the BrRLP31 gene plays a key positive regulation role in the clubroot resistance process of the Chinese cabbage, the disease resistance of cruciferae crops and other horticultural plants is improved by utilizing the gene and the homologous sequence thereof, and the gene and the homologous sequence thereof have important application value.

This utility model relates to the field of windproof isolation covers, specifically disclosing a windproof isolation cover for cruciferous hybrid breeding. It includes a first positioning rod, which is a round rod structure. Two symmetrically arranged first positioning rods constitute a first support unit. In this windproof isolation cover for cruciferous hybrid breeding, the device is supported by a first support unit and a second support unit. The two first positioning rods within the first support unit are connected by a first connecting rod. The second connecting rod is fixedly connected to the upper surface of the second positioning rod within the second support unit, further strengthening the support structure and enabling it to better resist wind. Simultaneously, fixing nails installed at the lower ends of each first and second positioning rod can be inserted into the ground for fixation. The equidistantly arranged fixing rods in a ring on the partition plate can rotate, further securing the device and preventing it from swaying or shifting in the wind, thus improving the device's wind resistance.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

The application belongs to the technical field of genetic engineering, and particularly relates to application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases. The application provides application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases, and the amino acid sequence of the AtPEN2 protein is shown as SEQ ID NO. 1. The application takes Arabidopsis thaliana as a model plant to verify the prevention and treatment effect of overexpression of AtPEN2 protein on clubroot, and the results show that the AtPEN2 protein overexpression plant can improve the resistance to clubroot, indicating that the coding gene AtPEN2 of the AtPEN2 protein can be applied to molecular breeding of clubroot, and provides a new gene resource for molecular breeding of cruciferous crops.

The present application provides a kind of Cardamine macrophylla Willd. Uniform polysaccharide, it is derived from the whole grass of Brassicaceae Pteryxia plant Cardamine macrophylla Willd., and the preparation method and purpose of the Cardamine macrophylla Willd. Uniform polysaccharide are provided.The present application realizes the efficient extraction of Cardamine macrophylla Willd. Uniform polysaccharide by single factor multi-level experiment combined with Box-Behnken central combination design-response surface method optimization of high pressure auxiliary low eutectic solvent extraction Cardamine macrophylla Willd. Uniform polysaccharide optimum extraction process parameter.The Cardamine macrophylla Willd. Uniform polysaccharide prepared in the present application is rich in galacturonic acid glycan and I type rhamnose galacturonic acid glycan acidic polysaccharide, has good antioxidant activity and immune regulation activity.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The utility model discloses germination accelerating equipment for cruciferous vegetable seeds, and relates to the technical field of vegetable seed germination accelerating, the germination accelerating equipment comprises a hollow column, electric heating plates are fixed on two sides of the inner wall of the hollow column, seed mixed soil is put into a heat conduction culture dish with holes, the inner wall of the heat conduction culture dish with holes slides on the outer wall of a sliding column, and the electric heating plates are fixed on the inner wall of the hollow column. A heat conduction culture dish with holes is placed in a hollow column, and the outer wall of an insertion block and the inner wall of the hollow column are clamped and mounted, so that the heat conduction culture dish with holes is stably mounted on the inner wall of the hollow column, and the situation that the seeds absorb water unevenly due to the fact that a worker manually adds a water source is avoided; the bottom end of the hollow column and the top end of the groove column are fixedly installed until the bottom end of the pore plate makes contact with the top end of the supporting block, a fan is controlled to blow air into the hollow column through the pore plate, cold air is dried and heated through an electric heating plate, and hot air makes contact with the heat conduction culture dishes with the holes.

The invention provides a method for preventing and controlling plant aphids, which comprises the following specific steps: selecting plant germinated seeds with roots and root length of 2-6 cm, and in the growth process of the plant germinated seeds, applying light for 8 hours and photoperiod treatment in a dark cycle mode for 16 hours; in the plant germinated seeds, the seed sources comprise beans and cruciferous vegetables; meanwhile, in the growth process of the germinated seeds of the plants, a boron-containing nutrient solution is prepared from the following components: 4mM of Ca (NO3) 2.4 H2O, 1mM of KH2PO4, 1mM of KCl, 2mM of MgSO4. 7H2O, 0.5 mM of K2SO4, 50mu M of FeSO4. 7H2O, 50mu M of EDTA.Na2, 10mu M of MnCl2, 5mu M of ZnSO4. 7H2O, 0.5 mu M of CuSO4. 5H2O, 1mu M of (NH4) 6Mo7O24. 4H2O, 35-70 mu M of H3BO3 and 0.5 mu M of CoCl2; nutrient elements are applied according to the following steps that (1) a first boron-containing nutrient solution is adopted in 1-5 days, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; and (2) after 6-15 days, adopting a second boron-containing nutrient solution, wherein the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The present invention belongs to the field of plant genetic engineering and discloses the use of a gene for promoting the accumulation of very-long-chain fatty acids in plant seeds. The gene fragment comprises a nucleotide sequence as shown in any one of SEQ ID No. 1 to SEQ ID No. 5, or a complementary sequence thereof, or a derivative nucleotide sequence with at least 95% homology to these nucleotide sequences and the same function. The carbon chain of the very-long-chain fatty acid has greater than 18 carbon atoms, and the gene fragment is capable of increasing the proportion of very-long-chain fatty acids in phosphatidylcholine (PC) and / or phosphatidylethanolamine (PE) in plant seeds. The present invention utilizes the LPAT2 gene (nucleotide sequence, for example, as shown in SEQ ID No. 1 to SEQ ID No. 5) from a cruciferous plant to increase the percentage of very-long-chain fatty acids in phosphatidylcholine and phosphatidylethanolamine in plant seeds, overcoming technical bottlenecks encountered in the current field.

The invention provides a breeding method of a triploid flowering Chinese cabbage variety, which comprises the following steps of: (1) hybridizing cabbage type rape serving as a female parent and flowering Chinese cabbage serving as a male parent to obtain a triploid hybrid F1 generation; (2) performing phenotype identification on the triploid hybrid F1 generation, and screening combinations which are early in bolting, synchronous in main stem and lateral branch development, obvious in subgenome heterosis and resistant to clubroot; (3) performing a multi-point planting test on the excellent triploid hybrid F1 to verify the yield and disease resistance of the flowering Chinese cabbage; and (4) breeding a special triploid flowering Chinese cabbage variety for seed production. The triploid flowering Chinese cabbage cultivated by the method has the following outstanding advantages of high yield, high economic value, strong disease resistance, multi-round picking and long market supply period. The method provided by the invention solves the problems of lack of special varieties of traditional brassica campestris and low yield of brassica campestris of a dual-purpose variety of brassica campestris, provides a new technical route for germplasm innovation of brassicaceous vegetables, and has important popularization and application values.

The application provides a Chinese cabbage root knot disease related gene BrSWEET14a, a construction method and application, belongs to the technical field of plant genetic engineering, and particularly relates to the following technical scheme. The CDS sequence of the Chinese cabbage BrSWEET14a is shown as SEQ ID No. 1. The gene is transformed into Col-type Arabidopsis thaliana through an Agrobacterium flower dipping transformation method, and a BrSWEET14a heterologous expression Arabidopsis thaliana strain is obtained. It is found that the T-DNA insertion mutant of the BrSWEET14a homologous gene Atsweet14 is more resistant to root knot disease than the Col-type, and the heterologous expression of the Chinese cabbage BrSWEET14a leads to the decrease of the disease resistance of Arabidopsis thaliana to the root knot fungus. It can be seen that the Chinese cabbage BrSWEET14a is closely related to the root knot disease, and the gene and the homologous genes of the gene in other cruciferous plants can be applied to the breeding of cruciferous plants and other horticultural plants, and have a good application prospect.

The application discloses a Higgins anthracnose pathogenic gene ChTHR1 and application thereof. The nucleotide sequence of the ChTHR1 gene is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.3. The application determines that the pathogenic gene ChTHR1 has a key role in spore production, the rate of appressorium formation, mycelium biomass, ultraviolet resistance, melanin production and melanin accumulation of Higgins anthracnose, and can be used as a drug target for drug design and screening against Higgins anthracnose. Higgins anthracnose spreads between leaf blades of Brassica parachinensis and other cruciferous vegetables in Guangdong by means of conidia, and the conidia are eliminated or inhibited to prevent the prevalence of plant anthracnose. The ChTHR1 gene is used as a target for drug development, a drug for preventing and treating anthracnose caused by Higgins anthracnose and reducing the pathogenicity of plant Higgins anthracnose is screened, and the effect of the drug is evaluated.

The invention discloses a base fertilizer containing a microbial agent for brassicaceous plant broccoli, and belongs to the technical field of microbial fertilizers. The base fertilizer is prepared by the following steps: by taking pig manure and straws as organic raw materials, pretreating the organic raw materials, and mixing the pretreated organic raw materials with a mixture containing neurospora crassa, pseudonocardia alnus, bacillus, streptomyces, paenibacillus mucilaginosus, phanerochaete chrysosporium, azospirillum, trichoderma harzianum, pseudomonas fluorescens and thiobacillus thiooxidans; through staged fermentation, humic acid powder and a trace element mixture are added during aging, and finally bentonite is added for granulation and drying. The base fertilizer can improve the soil structure, adjust microbial communities, inhibit pathogenic bacteria, improve the nutrient utilization rate through nitrogen fixation, phosphate solubilization and potassium solubilization, provide comprehensive nutrition in combination with humic acid and trace elements, promote broccoli root development, enhance stress resistance and realize double improvement of yield and quality; the problems of soil degradation and environmental pollution caused by long-term use of chemical fertilizers are solved, and the requirements of green agriculture development are met.

The invention discloses a method for identifying plutella xylostella-resistant germplasm resources of cruciferous vegetable crops. The identification method comprises an identification device and an identification method, the identification device is provided with a breathable net on a cover body of a culture dish, agarose, filter paper and moisturizing cotton are combined to construct a proper feeding environment for the plutella xylostella, and a field feeding process is truly simulated under indoor conditions. On the basis of the device, an insect resistance identification method is established, three-instar larvae of plutella xylostella are inoculated and cultured regularly, and larva death number, weight change and leaf damage data are obtained; and further constructing a germplasm resource identification method for resisting the plutella xylostella of the cruciferous vegetable crops, and carrying out quantitative analysis and grade judgment on the insect resistance of the cruciferous vegetable crops by taking the larva death rate, the body weight inhibition rate and the leaf damage rate as evaluation indexes. The identification device and method cooperate with each other to form a standardized cruciferous vegetable crop insect-resistant resource identification system.

The invention discloses a probe method real-time fluorescent PCR (polymerase chain reaction) rapid detection primer and probe combination for cruciferae bacterial phytophthora blight and a detection method, and belongs to the technical field of biological detection. According to the probe method real-time fluorescent PCR rapid detection method for the cruciferae bacterial phytophthora blight, DNA of a tested strain is used as a template, and the probe method real-time fluorescent PCR rapid detection is carried out by using a primer and probe combination. The detection method has the advantages of high specificity, high accuracy, high sensitivity, rapidness, high efficiency, quantitative analysis, simple operation, good repeatability and strong applicability.

The invention provides a long-term maintaining method for a cotton field diaphous babaskii population based on functional plant combination and space optimization and application of the long-term maintaining method, and belongs to the technical field of biological prevention and control of agricultural pests. According to the method, functional plants are planted in the cotton field in a mode of combining strip planting and functional patch planting; leguminous plants are planted on the periphery of the cotton field in a strip mode to form a plant buffer strip; functional plaques are arranged in a cotton field in a dispersed mode, and compositae plants, umbelliferae plants and cruciferous plants are planted in the functional plaques in a mixed mode. On the basis of functional plant combination and space optimization, after one-time release of the leptosphaeria babaskii, the population is continuously and stably maintained in the cotton field for many years, the field overwintering survival rate is larger than or equal to 71.3%, the parasitism rate to the phenacoccus solenopsis is stabilized to be 80% or above, the natural enemy leptosphaeria babaskii can be effectively used for preventing and controlling the phenacoccus solenopsis in the cotton field, and the pesticide use amount is reduced.

This invention belongs to the fields of agricultural plant pathology and plant protection technology, and relates to a clubroot bacterium Pb034 gene and its application in the control of clubroot disease in cruciferous crops. This invention found that reducing the expression of the clubroot bacterium Pb034 gene using host-induced gene silencing (HIGS) technology or exogenous double-stranded RNA delivery technology is beneficial for enhancing the resistance of cruciferous plants to clubroot disease. This invention provides a new approach and method for the control of clubroot disease in cruciferous crops, and has broad agricultural application prospects and market value.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

The application provides a kind of plant cell membrane vesicle and cerium-based nanoscale enzyme composite material, its structure includes plant cell membrane vesicle and the cerium-based nanoscale enzyme attached to its surface;Cerium ion of cerium-based nanoscale enzyme and the carboxyl group on the surface of plant cell membrane vesicle form coordination bond;Plant cell membrane vesicle is from cruciferous plant, and the carboxyl group on the surface includes free carboxyl group connected with the surface of plant cell membrane vesicle by-NH-CO-PEGn-structure;The mass ratio of cerium ion and plant cell membrane protein is 0.1-0.5:1.The application also provides a method for preparing the composite material, and the application of the composite material in sepsis treatment.The composite material of the application can specifically adsorb endotoxin and efficiently degrade endotoxin, and can be used for preventing / rescuing cell inflammation induced by endotoxin, and can also effectively alleviate organ failure of sepsis model mice.

The invention provides RPA (recombinase polymerase amplification) detection primers for colletotrichum gloeosporioides of green vegetables and application of the RPA detection primers and belongs to the technical field of agriculture. In order to solve the problems of long time consumption (2-3 hours), low sensitivity (5 ng / mu L) and dependence on precise equipment in the existing PCR detection, the invention provides RPA primers 56F1 (5 '-GAAAAGCGGCAT-3') and 56R1 (5 '-CGTTGGAAAATACA-3') of targeted pathogenic bacteria specific genes NC030956, as well as an RPA primer 62F1 / F1 and an RPA primer 62F2 / F2, and establishes a 39 DEG C constant-temperature 20-minute amplification system. The method realizes three major breakthroughs: 1, the detection sensitivity reaches 0.05 ng / mu L and is improved by 100 times compared with PCR (Polymerase Chain Reaction); 2, seven related pathogens such as colletotrichum nicotianae are strictly distinguished, zero cross reaction is realized, and the sample accuracy rate is 100%; 3, the whole process only needs 1.5 hours, the DNA crude extraction time of diseased leaves is 30 minutes, the RPA amplification time is 20 minutes, the electrophoresis detection time is 35 minutes, the cost is low, and a thermal cycler is not needed. The invention provides an on-site solution with the characteristics of rapidness, accuracy and saving for prevention and control of the cruciferous anthracnose, and solves the problem that the traditional technology cannot give consideration to both speed and sensitivity.

The application discloses a method for preparing nano-silver particles by using coleus extraction liquid and products and application thereof, and belongs to the technical field of green biosynthesis of nanometer materials, and comprises the following steps: (1) after pretreatment, the coleus is crushed, mixed with deionized water, stirred uniformly, and then allowed to stand, and the coleus extraction liquid is obtained after filtration; (2) the coleus extraction liquid obtained in step (1) is mixed with an AgNO3 solution to obtain a mixed solution, and then the nano-silver particles are obtained after further centrifugation, washing and vacuum freeze-drying. The nano-silver particles obtained by the method have small average particle size, uniform size and stable structure, can effectively inhibit the activity of multiple plant pathogenic bacteria such as Pseudomonas syringae pv. tomato, can destroy the cell structure to make the endosolutes exosmose, has good bactericidal effect, and can be used for preventing and treating bacterial black spot disease of cruciferous vegetables.