43 results about "Brassicaceae" patented technology

The application provides a method for preventing and controlling plant aphids, and the specific steps are as follows: selecting plant germination seeds that have grown roots and the root length is 2-6 cm, applying a light cycle treatment of 8h light and 16h darkness in the growth process of the plant germination seeds; in the plant germination seeds, the seed sources include legumes and cruciferous vegetables; and in the growth process of the plant germination seeds, a boron-containing nutrient solution containing the following components is used: Ca(NO3)2.4H2O 4mM, KH2PO4 1mM, KCl 1mM, MgSO4.7H2O 2mM, K2SO40.5mM, FeSO4.7H2O 50μM, EDTA.Na2 50μM, MnCl2 10μM, ZnSO4.7H2O 5μM, CuSO4.5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 35-70μM, CoCl20.5μM; the nutrient elements are applied according to the following steps: (1) the first boron-containing nutrient solution is used for 1-5d, and the concentration of all components in the first boron-containing nutrient solution is 20-30% of that of the boron-containing nutrient solution; (2) the second boron-containing nutrient solution is used for 6-15d, and the concentration of all components in the second boron-containing nutrient solution is 40-60% of that of the boron-containing nutrient solution. The method can effectively prevent and control plant aphids.

The invention relates to the technical field of biological prevention and treatment, and discloses application of metarhizium anisopliae and beauveria bassiana in preparation of a microbial agent for preventing and treating clubroot of cruciferae, the microbial agent is a complex microbial agent, and the complex microbial agent comprises metarhizium anisopliae and beauveria bassiana. According to the invention, the clubroot of cruciferae is prevented and controlled by utilizing the bacteriostatic activity and composite synergistic effect of metarhizium anisopliae and beauveria bassiana. The fermentation liquor of the metarhizium anisopliae and the beauveria bassiana can directly act on the plasmodiophora brassicae spores, the inactivation rate of the plasmodiophora brassicae spores is remarkably increased, and disease infection is blocked from the source. The problems that disease-resistant varieties lose efficacy due to diversity of physiological races of germs, agricultural management measures have a limited effect on long-term survival spores, and chemical pesticides pollute the environment and induce drug resistance are solved; and the preparation in the technical scheme is environment-friendly, has no residue and is not easy to induce drug resistance of germs.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP31 and application of the Chinese cabbage clubroot-resistant gene BrRLP31. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP31 is as shown in SEQ ID No. 1. Functional verification results show that after the expression of the endogenous BrRLP31 of the Chinese cabbage is inhibited by utilizing a VIGS technology, the resistance of the plant to clubroot is obviously reduced, and the disease index and the root swelling severity of the plant are obviously higher than those of a control material. On the other hand, the transgenic flowering cabbage plant over-expressed by the BrRLP31 is constructed by an agrobacterium-mediated method, and disease resistance identification proves that the heterologous over-expression of the BrRLP31 obviously improves the resistance of the plant to plasmodiophora brassicae. The results show that the BrRLP31 gene plays a key positive regulation role in the clubroot resistance process of the Chinese cabbage, the disease resistance of cruciferae crops and other horticultural plants is improved by utilizing the gene and the homologous sequence thereof, and the gene and the homologous sequence thereof have important application value.

This utility model relates to the field of windproof isolation covers, specifically disclosing a windproof isolation cover for cruciferous hybrid breeding. It includes a first positioning rod, which is a round rod structure. Two symmetrically arranged first positioning rods constitute a first support unit. In this windproof isolation cover for cruciferous hybrid breeding, the device is supported by a first support unit and a second support unit. The two first positioning rods within the first support unit are connected by a first connecting rod. The second connecting rod is fixedly connected to the upper surface of the second positioning rod within the second support unit, further strengthening the support structure and enabling it to better resist wind. Simultaneously, fixing nails installed at the lower ends of each first and second positioning rod can be inserted into the ground for fixation. The equidistantly arranged fixing rods in a ring on the partition plate can rotate, further securing the device and preventing it from swaying or shifting in the wind, thus improving the device's wind resistance.

The invention discloses a method for measuring the enzymolysis capacity of laccase on antinutritional factors and application, and belongs to the technical field of enzyme activity detection, the method refers to that the enzyme amount required by laccase for catalytically degrading sinapine under a certain condition is used as a measuring parameter of the enzyme activity of laccase in antinutritional factor degradation. According to the method, sinapine is innovatively used as an enzyme activity labeling substrate for enzyme activity determination, the enzymolysis capacity of laccase can be accurately reflected even if the reaction conditions of enzyme activity determination of different manufacturers are different, and the determination method not only can more accurately reflect the enzymolysis capacity of laccase on the antinutritional factors, but also can accurately determine the enzymolysis capacity of laccase on the antinutritional factors. The problem that labeling results are inconsistent when the recombinant laccase is labeled with an existing enzyme activity labeling method can also be solved, a methodological basis is provided for evaluation of the technical effect of reduction of anti-nutritional factors of cruciferae unconventional plant feed resources such as rapeseed dregs, and the method has extremely important application value and wide application prospects in the field of feed breeding.

This invention belongs to the fields of genetic engineering and plant cultivation technology, specifically relating to a method for inducing flavonoid synthesis in kale through selenium induction. BroMYB34 Genes and their applications. Specifically, this invention is the first to identify and clone a selenium-induced gene in kale. BroMYB34 The gene was used to elucidate its molecular function as a transcription factor regulating flavonoid synthesis through overexpression. BroMYB34 Genes can significantly increase the flavonoid content of kale and enhance its antioxidant capacity, providing an efficient and targeted genetic engineering approach for quality improvement of kale; at the same time, BroMYB34 Genes in cruciferous plants have potential functional conservation and can be extended to the quality improvement of various vegetables. This is of great significance for promoting the breeding and industrialization of functional vegetables and therefore has good practical application value.

The application belongs to the technical field of genetic engineering, and particularly relates to application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases. The application provides application of AtPEN2 protein and / or its coding gene AtPEN2 in preventing cruciferous plant diseases, and the amino acid sequence of the AtPEN2 protein is shown as SEQ ID NO. 1. The application takes Arabidopsis thaliana as a model plant to verify the prevention and treatment effect of overexpression of AtPEN2 protein on clubroot, and the results show that the AtPEN2 protein overexpression plant can improve the resistance to clubroot, indicating that the coding gene AtPEN2 of the AtPEN2 protein can be applied to molecular breeding of clubroot, and provides a new gene resource for molecular breeding of cruciferous crops.

This invention discloses a combined microbial agent and its application in combating clubroot disease, belonging to the field of microbial pesticide technology. By weight percentage, the combined microbial agent comprises 20%-30% Bacillus subtilis, 15%-25% Bacillus amyloliquefaciens, 10%-25% Lactobacillus fermentum, 10%-20% Isaac's yeast of Orientia, and 10%-20% Actinobacillus sieboldii. There is a synergistic effect among the strains, resulting in a significant effect on the control of clubroot disease, superior to conventional pesticides such as chlorothalonil. Using the combined microbial agent provided by this invention to control clubroot disease can reduce the amount of chemical pesticides used, exhibiting environmental friendliness and high efficiency. Furthermore, field trials have shown that treatment with the combined microbial agent of this invention reduces the incidence of clubroot disease in cruciferous crops while significantly increasing crop yield, resulting in economic benefits. The combined microbial agent of this invention is suitable for practical production and easy to promote and apply.

The utility model discloses germination accelerating equipment for cruciferous vegetable seeds, and relates to the technical field of vegetable seed germination accelerating, the germination accelerating equipment comprises a hollow column, electric heating plates are fixed on two sides of the inner wall of the hollow column, seed mixed soil is put into a heat conduction culture dish with holes, the inner wall of the heat conduction culture dish with holes slides on the outer wall of a sliding column, and the electric heating plates are fixed on the inner wall of the hollow column. A heat conduction culture dish with holes is placed in a hollow column, and the outer wall of an insertion block and the inner wall of the hollow column are clamped and mounted, so that the heat conduction culture dish with holes is stably mounted on the inner wall of the hollow column, and the situation that the seeds absorb water unevenly due to the fact that a worker manually adds a water source is avoided; the bottom end of the hollow column and the top end of the groove column are fixedly installed until the bottom end of the pore plate makes contact with the top end of the supporting block, a fan is controlled to blow air into the hollow column through the pore plate, cold air is dried and heated through an electric heating plate, and hot air makes contact with the heat conduction culture dishes with the holes.

The present disclosure provides the use of β-1,3-glucan synthase like 5 (GSL5) for the improvement of the clubroot resistance in cruciferous crops as well as related products development, belonging to the area of molecular engineering for plant breeding. GSL5 gene is highly conserved in cruciferous plants and mutation of GSL5 can confer a broad-spectrum and high clubroot resistance to the cruciferous crops, demonstrating that molecular engineering of GSL5 can achieve the improvement of the clubroot resistance in cruciferous crops. The present disclosure provides a key gene and techniques for the improvement of the clubroot disease resistance and durable prevention and control of the clubroot disease in cruciferous crops.

The application discloses a Higgins anthracnose pathogenic gene ChTHR1 and application thereof. The nucleotide sequence of the ChTHR1 gene is shown as SEQ ID NO:1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.3. The application determines that the pathogenic gene ChTHR1 has a key role in spore production, the rate of appressorium formation, mycelium biomass, ultraviolet resistance, melanin production and melanin accumulation of Higgins anthracnose, and can be used as a drug target for drug design and screening against Higgins anthracnose. Higgins anthracnose spreads between leaf blades of Brassica parachinensis and other cruciferous vegetables in Guangdong by means of conidia, and the conidia are eliminated or inhibited to prevent the prevalence of plant anthracnose. The ChTHR1 gene is used as a target for drug development, a drug for preventing and treating anthracnose caused by Higgins anthracnose and reducing the pathogenicity of plant Higgins anthracnose is screened, and the effect of the drug is evaluated.

The invention discloses a method for identifying plutella xylostella-resistant germplasm resources of cruciferous vegetable crops. The identification method comprises an identification device and an identification method, the identification device is provided with a breathable net on a cover body of a culture dish, agarose, filter paper and moisturizing cotton are combined to construct a proper feeding environment for the plutella xylostella, and a field feeding process is truly simulated under indoor conditions. On the basis of the device, an insect resistance identification method is established, three-instar larvae of plutella xylostella are inoculated and cultured regularly, and larva death number, weight change and leaf damage data are obtained; and further constructing a germplasm resource identification method for resisting the plutella xylostella of the cruciferous vegetable crops, and carrying out quantitative analysis and grade judgment on the insect resistance of the cruciferous vegetable crops by taking the larva death rate, the body weight inhibition rate and the leaf damage rate as evaluation indexes. The identification device and method cooperate with each other to form a standardized cruciferous vegetable crop insect-resistant resource identification system.

The invention provides a long-term maintaining method for a cotton field diaphous babaskii population based on functional plant combination and space optimization and application of the long-term maintaining method, and belongs to the technical field of biological prevention and control of agricultural pests. According to the method, functional plants are planted in the cotton field in a mode of combining strip planting and functional patch planting; leguminous plants are planted on the periphery of the cotton field in a strip mode to form a plant buffer strip; functional plaques are arranged in a cotton field in a dispersed mode, and compositae plants, umbelliferae plants and cruciferous plants are planted in the functional plaques in a mixed mode. On the basis of functional plant combination and space optimization, after one-time release of the leptosphaeria babaskii, the population is continuously and stably maintained in the cotton field for many years, the field overwintering survival rate is larger than or equal to 71.3%, the parasitism rate to the phenacoccus solenopsis is stabilized to be 80% or above, the natural enemy leptosphaeria babaskii can be effectively used for preventing and controlling the phenacoccus solenopsis in the cotton field, and the pesticide use amount is reduced.

The invention relates to the technical field of plasmodiophora brassicae inoculation, in particular to an artificial inoculation method for plasmodiophora brassicae immediate infection and application, and the method specifically comprises the following steps: S1, accelerating germination: accelerating germination of seeds to obtain seedlings; s2, grinding bacteria and preparing a bacteria solution; s3, inoculation: performing field planting on the seedlings obtained in the step S1, and then performing injection inoculation on the overground parts of the seedlings by using the bacterial liquid prepared in the step S2; s4, seedling stage management; s5, disease investigation. According to the method, the cognitive limitation of traditional two-step infection of root hair infection and cortex diffusion is broken through, a brand-new inoculation technical path is constructed, and a new idea and method for inoculating plasmodiophora brassicae of cruciferae are developed; the defects that a traditional underground inoculation method is fuzzy in infection time sequence, uncontrollable in inoculation metering, low in inoculation efficiency and poor in pathogenesis stability due to large bacterial quantity fluctuation are fundamentally overcome.

The application discloses application of NPR1 protein and related biological materials thereof in regulating resistance of plants to clubroot disease, and belongs to the technical field of genetic engineering. The technical problem to be solved by the application is how to regulate resistance of plants to Plasmodiophora brassicae. Specifically, the technical problem to be solved by the application is how to regulate resistance of cruciferous plants to Plasmodiophora brassicae. To solve the above technical problem, the application provides application of NPR1 protein or biological materials related to the NPR1 protein in regulating resistance of plants to clubroot disease. The application discloses, for the first time, application of NPR1 protein and a coding gene thereof in regulating resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae. Research on the function of NPR1 and application of the function in cruciferous plant breeding are of great significance to the mechanism of resistance of cruciferous plants to clubroot disease and / or Plasmodiophora brassicae and to resistance breeding.

The application provides a kind of plant cell membrane vesicle and cerium-based nanoscale enzyme composite material, its structure includes plant cell membrane vesicle and the cerium-based nanoscale enzyme attached to its surface;Cerium ion of cerium-based nanoscale enzyme and the carboxyl group on the surface of plant cell membrane vesicle form coordination bond;Plant cell membrane vesicle is from cruciferous plant, and the carboxyl group on the surface includes free carboxyl group connected with the surface of plant cell membrane vesicle by-NH-CO-PEGn-structure;The mass ratio of cerium ion and plant cell membrane protein is 0.1-0.5:1.The application also provides a method for preparing the composite material, and the application of the composite material in sepsis treatment.The composite material of the application can specifically adsorb endotoxin and efficiently degrade endotoxin, and can be used for preventing / rescuing cell inflammation induced by endotoxin, and can also effectively alleviate organ failure of sepsis model mice.

The invention relates to the technical field of pesticides, and discloses an insect pest prevention and control method for brassicaceous vegetables. The method comprises the following steps: raising seedlings of cruciferous vegetable seeds, and controlling insect pests by using chemical pesticides in the vegetable growth process. Main insect pests of cruciferous vegetables include cabbage caterpillars, plutella xylostella, aleyrodid, aphids, beet armyworms, grubs, prodenia litura, mamestra brassicae, agrotis ypsilon, phyllotreta striolata, radish borer, simian insects and the like, when the insect pests occur, chemical pesticides are used for controlling the insect pests, the chemical pesticides comprise fenpropathrin and chlorbenzuron as effective components, and the chemical pesticides are used for controlling the insect pests. The suspending agent composition is prepared by adding an auxiliary agent and a synergist into the effective components, is used for preventing and treating brassicaceous vegetable insect pests, especially has a very good effect on cabbage plutella xylostella, not only solves the problem of insect pests in the cabbage growth period, but also reduces the pesticide use amount, solves the problems that pesticide residues are easy to exceed the standard and the safety interval is long, and has a good application prospect. And the purposes of pesticide reduction and synergism are achieved.

This application discloses the application of the EDS1 protein and its encoding gene in regulating plant disease resistance, belonging to the field of genetic engineering technology. The technical problem this application aims to solve is: how to regulate clubroot resistance in plants. To this end, this application provides the application of the EDS1 protein, or substances regulating the expression of the EDS1 protein encoding gene, or substances regulating the content of the EDS1 protein, in regulating plant clubroot resistance; the EDS1 protein can be a protein with the amino acid sequence being sequence 2. This application is the first confirmation of EDS1's participation in the *Cladophora brassicae* infection cycle, and the obtained susceptible materials can be utilized as a potential breeding resource. The discovery and enrichment of breeding resources will greatly accelerate the breeding process of clubroot-resistant cruciferous plants and strengthen disease control. Furthermore, clubroot resistance can be achieved by overexpressing EDS1 in cruciferous plants through transgenic or other means.

The invention discloses a purple cabbage-derived eccDNA molecule for identifying new germplasm of preserved szechuan pickle and application of the purple cabbage-derived eccDNA molecule. The eccDNAs molecule has the potential of cross-species transfer, and can be used as a natural genetic vector to introduce excellent genetic information of distant species into cultivated species. The invention successfully realizes stable inheritance of eccDNAs molecules of distant materials such as purple cabbage in new germplasm of preserved szechuan pickle. Specifically, an eccDNA molecule (chr3: 46317085-46317645) specifically expressed in the new germplasm of the preserved szechuan pickle is identified, and the molecule carries an ERF4 gene from purple cabbage and has a conservative AP2 / ERF structural domain. According to the present invention, the existence and the expression specificity of the eccDNA molecule are confirmed through circle-seq sequencing, reverse PCR and DH5alpha competent cell positive clone verification, and the corresponding specific PCR detection primer pair is provided; the eccDNA molecule is inherited in the new germplasm sexual progeny of the preserved szechuan pickle, the expression of the eccDNA molecule can promote the leaf shape to be converted into an oval shape (similar to purple cabbage) from an incised shape, the leaf thickness is increased, and meanwhile, the drought resistance is remarkably improved. The invention provides a novel method for genetic improvement of cruciferous crops based on eccDNAs mediation, and provides a novel thought and technical approach for molecular breeding of preserved szechuan pickles and other related crops.

The invention provides a method for improving clubroot resistance of cruciferous plants. It is found for the first time that probenazole treatment can improve the resistance of Chinese cabbages to clubroot and inhibit the vitality of plasmodiophora brassicae spores. The morbidity and the disease index of the Chinese cabbage clubroot can be reduced through pretreatment with probenazole of different concentrations and different dosage forms, the disease development is delayed, and adverse effects on the growth of Chinese cabbages cannot be caused. The probenazole suspending agent and the nanocapsule suspending agent prepared by the invention have outstanding prevention and control effects on clubroot of cruciferous plants.

This invention relates to a rapeseed BnCDY201 gene and its application in regulating the flowering and vegetative growth stages of cruciferous plants, belonging to the field of plant genetic engineering technology. The nucleotide sequence of the BnCDY201 gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. The overexpression vector of the BnCDY201 gene constructed in this invention was transformed into Arabidopsis thaliana. The resulting transgenic plants exhibited significantly delayed flowering and a significantly increased number of rosette leaves, demonstrating the gene's function in prolonging the vegetative growth stage and delaying flowering. This provides a new genetic resource and effective tool for regulating plant flowering and vegetative growth through genetic engineering, and has significant application value in crop breeding.

The application discloses an interference sequence for improving drought resistance of Brassica napus and application thereof, wherein the interference sequence targets BnaA7.ARF17 gene in the Brassica napus, and the interference sequence can reduce the expression level of the gene, and the interference sequence is an antisense sequence or an RNA interference hairpin structure of SEQ ID NO:1. By targeting the BnaA7.ARF17 gene in the Brassica napus and using the antisense sequence or the RNA interference hairpin structure thereof, the expression level of the gene can be accurately and efficiently reduced, so that the drought resistance of the Brassica napus is significantly improved, an effective gene regulation strategy for coping with drought stress is provided, the yield and quality of the Brassica napus under drought conditions are improved, and the biological technology means for improving the drought resistance of Brassica plants is expanded.

The invention belongs to the technical field of agriculture and biology, discloses application of arabidopsis thaliana GALT4 in regulation and control of pollen development, and verifies that the arabidopsis thaliana GALT4 gene is a plant male fertility key regulation and control gene with high pollen specificity expression, and the CDS sequence is shown as SEQ ID NO.1 for the first time. Polarity elongation of the pollen tube is maintained by regulating and controlling spatial and temporal distribution of methyl esterified pectin in the pollen tube. Knockout or silencing of the homologous gene in a plant can lead to reduction of pollen viability, abnormal development of pollen cell nucleus, malformation of pollen morphology, slowing of pollen germination rate and hindering of pollen tube polarity elongation. The invention provides a brand new molecular target for plant pollen morphological development and pollen tube growth regulation and control, can be used for fertility regulation and control and hybrid seed production optimization of cruciferous crops, and has important theoretical research value and industrial application prospect.

The invention discloses a microorganism for preventing and treating clubroot of cruciferae and a preparation method and application of a fungicide of the microorganism, and relates to the technical field of biological prevention and treatment of plant diseases, the microorganism is screened out through a microbial metabolome and verified, the microorganism is matched with a microorganism resource library to form nocardia-like actinomycetes (1A6), and the use concentration of the microorganism is OD600 = 1. According to the microorganism for preventing and treating the clubroot of the cruciferae and the preparation method of the microbial inoculum of the microorganism, the strain is screened out from contrastive analysis of root swelling tissues of green vegetables and soil metabolome, and the microorganism is applied to preventing and treating the clubroot of the cruciferae, so that the clubroot prevention and control effect is effectively achieved; and the method has the advantages of low pollution and low residue.

The invention discloses application of an arabidopsis thaliana gene AT1G12064 in arabidopsis thaliana breeding and a method, and belongs to the field of gene engineering. Through verification, the gene negatively regulates and controls the size and weight of arabidopsis seeds and the number of grains per pod, and positively regulates and controls the pod length, the flowering period and the growth period. A mutant of the gene is knocked out, the seed length, width, area, grain weight, grain number per pod and single plant yield are remarkably increased, the flowering period and the growth period are shortened, and early maturing and high yield are achieved. New gene resources and technical support are provided for early-maturing and high-yield breeding of cruciferous crops such as arabidopsis thaliana, oilseed rape and Chinese cabbage, and the application prospect is wide.

This invention relates to the fields of agricultural biotechnology and environmental remediation technology, specifically to a novel, highly efficient, and safe formulation for promoting plant growth and degrading soil phthalates, and its application. The formulation comprises (R)-5-hydroxymethyldihydrofuran-2-one and water. Experiments have shown that (R)-5-hydroxymethyldihydrofuran-2-one, at a concentration of 0.01 mM, has a significant growth-promoting effect on cruciferous vegetable Chinese flowering cabbage (Pachira aquatica), while simultaneously accelerating the degradation of dibutyl phthalate (DBP) in the soil, achieving a dual function. Experiments also show that applying 0.01 mM of A-like to DBP-contaminated soil significantly increases the plant height, root length, and biomass of Chinese flowering cabbage, promotes earlier flowering, and reduces the residual amount of DBP in the soil by 77.2%, with a degradation promotion rate exceeding 62%.

The invention discloses an oilseed rape BnaC03g38070D gene and an application of the oilseed rape BnaC03g38070D gene in promoting flowering of cruciferous plants. According to the invention, SNP markers of 326 rape populations are respectively used for GWAS analysis with flowering phenotypes under long-day and short-day conditions, a candidate gene BnaC03g38070D is screened at a C03 associated site, the coding region of the candidate gene BnaC03g38070D is mutated due to SNP variation (G is converted into C) in the coding region of the candidate gene BnaC03g38070D, the site is significantly associated with the flowering traits of the rape, and the gene BnaC03g38070D can be used for identifying the flowering traits of the rape. And the expression level of the gene is also highly related to the flowering traits of the rape. The BnaC03g38070D gene is heterologously expressed in arabidopsis thaliana, the flowering time of an obtained homozygous transgenic line is obviously earlier than that of a wild type, and the total number of leaves is obviously reduced, which indicates that the expression of the gene can promote the flowering of arabidopsis thaliana. Therefore, the gene can be applied to molecular breeding of the flowering period character of cruciferae and flowering period regulation and control.

The invention discloses a Chinese cabbage clubroot-resistant gene BrRLP30 and application thereof. The nucleotide sequence of the Chinese cabbage clubroot-resistant gene BrRLP30 is as shown in SEQ ID No.1. The invention further discloses a preparation method of the Chinese cabbage clubroot-resistant gene BrRLP30. After the endogenous BrRLP30 gene of the Chinese cabbage is silenced by a VIGS technology, the susceptibility of the plant to clubroot is increased, the disease index is obviously higher than that of a control group, and the root swelling symptom is aggravated. The gene is transformed into a Chinese flowering cabbage plant through an agrobacterium flower immersion transformation method to obtain a BrRLP30 heterologous overexpression strain, and a disease resistance identification result shows that the overexpression of BrRLP30 significantly enhances the resistance of the plant to clubroot. Therefore, the gene BrRLP30 is closely related to the clubroot disease, the gene and the homologous gene of the gene in other cruciferous plants can be applied to breeding of the cruciferous plants and other horticultural plants, the disease resistance of the plants can be improved, and the gene BrRLP30 has a good application prospect.

This invention discloses a method for the propagation and renewal of *Avicennia marina* germplasm resources. *Avicennia marina* is grafted onto radish rootstock to achieve propagation and renewal. This one-step tissue culture regeneration method eliminates the need for explant cutting, complex culture media, the addition of exogenous hormones, and multiple culture media changes, saving manpower and financial resources and preventing contamination risks during culture media changes. Compared with existing technologies, this root-transplantation cultivation method effectively solves the problem of *Avicennia marina* roots being unsuitable for soil, leading to early plant death and hindering propagation and renewal. Furthermore, utilizing radish roots, which are highly resistant to clubroot disease, solves the problem of clubroot disease and even plant death. In addition, radish roots can be harvested and stored for overwintering, maintaining a very high survival rate the following year. Compared to other cruciferous crops such as cabbage and mustard, using radish as a root donor facilitates overwintering and the subsequent greening and reproductive growth of *Avicennia marina* the following year.

The invention discloses bacillus intergenus MS443 and application thereof, and belongs to the technical field of microorganisms. The intergeneric bacillus sp. MS443 is preserved in the China Center for Type Culture Collection, and the preservation number of the intergeneric bacillus sp. MS443 is CCTCC (China Center for Type Culture Collection) NO: M 2025280. The strain has a heterotrophic nitrification function, can effectively convert ammonium nitrogen into nitrate nitrogen, can be applied to soil nitrogen conversion, and converts ammonium nitrogen accumulated in soil into nitrate nitrogen which is more easily absorbed by plants. On the other hand, due to the fact that the utilization efficiency of plants on nitrogen is improved, the yield is improved, and the strain shows a remarkable growth promoting effect on various crops including solanaceae (such as tomatoes and peppers), gramineae (such as corn), malvidae (such as cotton) and cruciferae (such as flowering cabbage) plants and can be applied to agricultural production to improve the nitrogen fertilizer utilization efficiency and the crop yield; the use amount of chemical fertilizer can be reduced.