237 results about "Brassicaceae" patented technology

Lipo Chitooligosaccharide (LCO) [NodBj-V(C18:1,Mefuc)] isolated from Bradyrhizobium japonicum strain 532C was able to stimulate seed germination/seedling emergence, or in the case of potato, sprouting, of a number of crop plants representing eight distantly related plant families (Poaceae, Fabaceae, Brassicaceae, Cucurbitaceac, Malvaceae, Asteraceae, Chenopodiaceae and Solanaceae) of plants, at 25 and/or at 15° C. It also promoted sprouting potato minitubers. Other LCOs [NodRM-V(C_16:2,5) and LCO from R. leguminosarum] were also shown to also display growth-promoting effects on the tested crop plants. The compositions comprising at least one LCO are shown to be effective in promoting growth under both laboratory and field conditions. The invention thus also relates to methods for promoting seed germination and/or seedling emergence and/or growth of plants comprising subjecting the seeds and/or plants to an effective amount of an agricultural composition comprising at least one LCO.

The present invention provides methods for growing plants to result in increased concentrations of one or more glucosinolates. The plant may be a member of the order Capparales, such as a Brassicaceae, a Capparaceae, or a cultivar thereof. The methods include exposing a plant to an altered radiation condition, a water deficit condition, an altered growth temperature, an altered level of sulfur and/or nitrogen, or a combination thereof.

The invention relates to a biological agent against crucifer club root and application thereof, belonging to the technical field of bio-pesticide. The strain for production is Bacillus subtilis XF-1, whose preserving number is CGMCC NO.2357. The stain has the characteristics as follows: (1) the primary colony on the LB culture substrate is white and round having a wet surface; the later colony is light yellow having uneven edge with dry and crimple surface; observed from the microscope, the strain is short-bar shaped and movable with spore, peritricha and dimension of 0.7 - 0.8 * 2.0-2.4 mum; (2) the strain is Gram-positive and aerobic and makes use of glycogen, sugar, citrate, gelatin hydrolysate, starch and casein, but does not make use of cellulose, tyrosine and catalase positive; (3) the stain has the function of sterilization, disease prevention, and yield improvement. The embodiment of the invention is as follows: using the test tube of Bacillus subtilis XF-1 stain, shake cultivation, and culture solution for fermentation to prepare biological agent, then applying the biological agent to the rhizosphere soil of crucifer crops, thereby having good effect in preventing and treating, and simple production.

Disclosed is a large-scale breeding method for breeding mummified aphids and adult bees of aphidiusgifuensis by yellow-green myzuspersicae. The yellow-green myzuspersicae with large bodies serve as host aphids of aphidiusgifuensis, and parent aphid disease-resistant leafy cruciferous green vegetables, radishes or sweet blue flowers serve as host plants of the yellow-green myzuspersicae, so that the mummified aphids and the adult bees of the aphidiusgifuensis are artificially bred on a large scale. The method solves the problems that the bodies of the host aphids are small, the mummified aphids are difficultly collected, the eclosion rate of the aphidiusgifuensis is low, successful egg laying amount is small and industrialized production is not easily realized when the aphidiusgifuensis is artificially bred. Collecting and breeding technology of the host aphids and species of the aphidiusgifuensis is provided in the artificially breeding process, the technology of host plant cultivation, inoculation breeding of the aphids and breeding of the mummified aphids and the adult bees of the aphidiusgifuensis is provided, the method has the advantages of short period, high efficiency, high breeding speed and low cost, the mummified aphids of the aphidiusgifuensis can be largely bred, the mummified aphids and the adult bees of the aphidiusgifuensis are produced on a large scale, and the myzuspersicae, English grain aphids and cotton aphids can be effectively prevented in the technical field of biological prevention.

The invention provides a method for preparing indigo naturalis, comprising the following steps of: a, placing leaves or stem leaves of strobilanthus (acanthaceae), indigo (polygonaceae) or woad (crucifer) into water with the pH value of 3.5-7 at the temperature of 25-60 DEG C, the mass ratio of water to the fresh leaves is 8-15, soaking for 24-48 hours, and filtering and removing leaf slag to obtain filtrate; b, introducing air into the filtrate for 0-30min, adding alkaline to regulate the pH value to be 9-10.5, introducing the air for 30-240min, adding lime milk, stirring to be uniform and standing, filtering to obtain precipitate, drying to obtain crude indigo, and refining the crude indigo to finally obtain the indigo naturalis. In the crude indigo prepared by adopting the method provided by the invention, contents of indigo blue and indirubin are higher, and product quality is good.

A process of aqueous protein extraction from Brassicaceae oilseed meal, such as canola, commercial canola meal or yellow mustard, to obtain a napin-rich protein extract, a cruciferin-rich protein extract, and a low-protein residue. The process comprising the steps of performing aqueous extraction of the Brassicaceae oilseed meal at a pH of from about 2.5 to about 5.0 to obtain a soluble napin-rich protein extract and a cruciferin-rich residue followed by performing aqueous extraction of the cruciferin-rich residue to obtain a soluble cruciferin-rich protein extract and a low-protein residue. The cruciferin-rich residue may be treated with cell wall degrading enzymes to obtain a cruciferin-rich fraction The cruciferin-rich protein products may be substantially free of napin protein and may be useful as a non-allergenic food product for human consumption.

Products containing juices and or extracts from Brassicaceae plants are notorious for their obnoxious odor. This odor is a result of the myrosinase-glucosinolate system found in the family of plants. The present invention relates to Brassicaceae products (particularly red cabbage pigments) and provides treatments using enzyme(s) and chromatography to prevent, or significantly reduce the formation of off-odors.

The present invention relates to the isolation, purification, characterization and use of a mitochondrial pyruvate dehydrogenase kinase (PDHK) gene [SEQ ID NO:1](pYA5; ATCC No 209562) from the Brassicaceae (specifically Arabidopsis thaliana). The invention includes isolated and purified DNA of the stated sequence and relates to methods of regulating fatty acid synthesis, seed oil content, seed size/weight, flowering time, vegetative growth, respiration rate and generation time using the gene and to tissues and plants transformed with the gene. The invention also relates to transgenic plants, plant tissues and plant seeds having a genome containing an introduced DNA sequence of SEQ ID NO:1; or a part of SEQ ID NO:1 characterized in that said sequence has been introduced into an antisense or sense orientation, and a method of producing such plants and plant seeds. The invention also relates to substantially homologous DNA sequences from plants encoding proteins with deduced amino acid sequences of 25% or greater identity, and 50% or greater similarity, isolated and/or characterized by known methods using the sequence information of SEQ ID NO:1, and to parts of reduced length that are still able to function as inhibitors of gene expression by use in an anti-sense, co-suppression or other gene silencing technologies.

The invention discloses an anti-bacterial protein PBR1 as well as a preparation method and application thereof, and relates to the technical field of molecular biology and biological pesticides. The amino acid sequence of the anti-bacterial protein PBR1 is shown as SEQ ID NO.1. The anti-bacterial protein PBR1 has a superior effect on preventing and curing plant diseases such as cruciferous plasmodiophoromycetes, paddy rice magnaporthe oryzae, curvularia lunata, fusarium oxysporum f. sp. dianthi, fusarium oxysporum vasinfectum, konjac foot rot bacteria, panax notoginseng root rot pathogen, pomegranate wilt pathogen, gaeumannomyces gramini, cylindrosporium pomi brooks, alternaria alternata and/or botrytis cinerea. A pesticide taking the anti-bacterial protein PBR1 as an active ingredient is a novel environment-friendly biological pesticide, is safe to people and livestock, protects the environment, and has a favorable development prospect.

The invention discloses a heavy metal adsorbent and a preparation method and application thereof. The heavy metal adsorbent is biological charcoal obtained by air-drying and pyrolyzing cruciferous plants in a grinding mode, wherein the cruciferous plants comprise leaf mustards, radishes, oilseed rape, Chinese cabbages, cabbages, shepherd's purse, wild cabbages and the like. Through tests, it is found that the cruciferous plants contain more sulfhydryl compounds, and the biological charcoal with a sulfydryl group can be obtained after the sulfhydryl compounds are pyrolyzed, can generate adsorbing and chelating effects with heavy metal ions and has the high affinity with positive ions of heavy metals such as Cd, Cu, Mn, Ni and Zn; the waste cruciferous plant bodies such as the Chinese cabbages and the leaf mustards are collected to be air-dried and pyrolyzed to prepare the biological charcoal, therefore, the discharge amount of garbage can be decreased, reutilization of waste resources is achieved, the high heavy metal adsorption capacity is achieved, and wide application prospects in heavy metal-containing water body treatment, heavy metal-contaminated soil remediation and permeable reactive barrier packing are achieved.

The invention discloses a cabbage type rape Ogura cytoplasmic male sterility (OGU CMS) restorer and an obtained derivative restorer material after the cabbage type rape Ogu CMS restorer is transferred, wherein the preservation number of the Chinese microbial culture preservation management committee center for general microbiology is CGMCC No. 6001, and the plant name is Ogu CMS restorer CLR650. A transformation method of the cabbage type rape OgU CMS restorer comprises the following steps: using a cruciferae resource containing Ogu CMS exogenous restoring genes as a restoring gene donor parent, utilizing a grafting technology to overcome distant hybridization reproductive dysfunction, leading intergeneric radish restoring genes into cabbage type rapes, continuously culturing fertile plants with chlorosis, and obtaining the restorer CLR650 which can be fertile in selfing and test-crossing after screening, selfing and backcross screening for multiple generations. The restorer material is low in cost, good in safety and capable of being applied to a cabbage type rape Ogu CMS pollination system.

The invention relates to a breeding method for colored-flower rape used for creative agriculture. The method comprises the following steps: selecting a mainly cultivated rape variety with superior growth vigor, medium height, good lodging resistance and disease resistance and large petals as a female parent and a cruciferous plant of varieties with colored petals as a male parent, carrying out hybridization and selecting plants with plant properties similar to those of the female parent rape and flower color properties similar to those of the male parent in segregative generations so as to obtain F0 seeds; sowing the F0 seeds, selecting the main inflorescence of excellent individual plants and reserving seeds thereof to obtain F1 seeds; then carrying out mixed seeding of the F1 seeds; starting with F2 plants, selecting plants with target flower colors in an initial flowering stage, carrying out selfing, bagging and seed reserving, and eliminating individual plants of an original rape flower color variety; and carrying out continuous selfing and separating until in few districts, a variety of a same target flower color appears in the whole district after F5 or F6, and carrying out curtain enclosing, serlfing and seed reserving. With the method provided by the invention, peasants both harvest a product (i.e., rapeseeds) and sell the landscape, and the environment is beautified.

The invention belongs to the technical field of agricultural sustainable development, relates to preparation of organic preparations and in particular relates to an organic preparation for controllingcruciferous vegetable club root and application thereof. The organic preparation is composed of organic raw materials and decomposition-promoting ingredients, wherein the organic materials include but not limited to one or more of crop straws, saw dust and other solid organic materials and molasses, alcoholic fermentation liquor and other liquid organic materials; the decomposition-promoting ingredients include but not limited to one or more of special microbes and organic catalysts; and a ratio (mass) of the organic raw materials to the decomposition-promoting ingredients is (95-100):(5-0).The invention further discloses a preparation method and application of the organic preparation. The organic preparation disclosed by the invention is ecologically environment-friendly and is applicable to soil with high incidence of club root after continuous cropping of various cruciferous vegetables. The quantity of plasmodiophora brassicae woron in soil and the incidence rate of the club rootof crops can be effectively reduced after treatment, the sterilization rate and the club root control rate respectively reach 60-90%, and the yield of the crops is obviously increased.

The invention provides methods and compositions for heavy metal phytoremediation, including plants which are genetically engineered to overexpress glutamylcysteine synthetase (ECS) and thereby provide enhanced heavy metal accumulation. In various embodiments, the plants comprise a gene encoding ECS operably linked to a heterologous promoter, the plant is a member of the Brassicaceae family. In general, the methods comprise the steps of growing such plants in a medium such as soil or water comprising a heavy metal, under conditions wherein ECS is overexpressed, whereby the plant provides enhanced accumulation of the heavy metal, whereby the heavy metal content of the medium is decreased.

The invention discloses isaria fumosorosea and bacillus thuringiensis wettable powder. The wettable powder comprises the following ingredients in percentage by mass: 8%-20% of isaria fumosorosea and bacillus thuringiensis, 1-8% of wetting agent, 1-12% of dispersing agent, 0.1-0.5% of ultraviolet protective agent, and the balance of carriers. The weight part ratio of the isaria fumosorosea to bacillus thuringiensis is (10:0.4) to (10:1). The isaria fumosorosea is isaria fumosorosea strain SCAU-IFCF02 which is preserved in CCTCC (China Center for Type Culture Collection) at November 1rst, 2013, with the preservation number of CCTCC NO:M2013526. The bacillus thuringiensis is subsp. Kurstaki. The wettable powder is fast and efficient, has no pesticide residues, postpones the occurrence of pest resistance to insecticide, is environment-friendly, and especially has a good prevention effect on pests on brassicaceous vegetables of plutella xylostella and the like.

The invention discloses a cruciferous plant nitrilase mutant, and belongs to the technical field of biological engineering. According to the cruciferous plant nitrilase mutant, peptide fragments of sites 225-285 of the amino acid sequences of an arabis alpina nitrilase are embedded into a turnip nitrilase lacking the corresponding peptide fragments to obtain a turnip/arabis alpina nitrilase chimera, and then a coding gene of the chimeric BaNIT is subjected to site-specific saturation mutation to obtain the plant nitrilase mutant, and the plant nitrilase mutant is one or more of the following:(1) L at the site 223 mutates into Q; (2) H at the site 263 mutates into D; and (3) Q at the site 279 mutates into E. The catalytic activity of the plant nitrilase mutant is increased by 2.23 times, the solubility of a recombinant protein is greatly improved, the enantioselectivity E value is maintained at 400 or above, and the plant nitrilase mutant has good application prospects in efficient catalysis of racemic isobutylsuccinonitrile to synthesize (S)-3-cyano-5-methylhexanoic acid.

The invention relates to methods and compositions for modulating properties of fruit dehiscence in plants such Brassicaceae plants, specifically to improved methods and means for reducing seed shattering in Brassicaceae plants, particularly the Brassicaceae plants grown for oil production, to a degree which is agronomically important.

The invention provides an application method of bacillus amyloliquefaciens Bamm22 for preventing and treating plasmodiophora brassicae. The method comprises the following two methods: the method I comprises: carrying out root-irrigation treatment on a cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 80-100 ml per time per strain, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fifteenth day after transplanting; the method II comprises: carrying out spraying treatment on the cruciferae crop by a bacillus amyloliquefaciens Bamm22 bacterial liquid with the amount of 150-200 ml per square meter, and carrying out treatment for at least three times, wherein the treatment times are the first day, the seventh day and the fourteenth day after seeding. The bacillus amyloliquefaciens Bamm22 provided by the invention is simple in using method, the application concentration of the bacterial liquid is less and the application dosage of the bacterial liquid is less.

The invention discloses an insecticidal composition containing sulfoxaflor and carbamates. The insecticidal composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is the sulfoxaflor; the active ingredient B is any of the following insecticides: carbaryl, indoxacarb, metolcarb, fenobucarb, and isoprocarb; and the weight ratio of the active ingredient A to the active ingredient B is 1-50 percent:1-80 percent. The composition can control various insect pests, has a remarkable synergistic effect, widens a bactericidal spectrum, and has relatively high activity on cotton bollworms, aphids, rice planthoppers, rice leafhoppers, and Cnaphalocrocis medinalis as well as Plutella xylostella, cabbage caterpillars and beet armyworms on cruciferous vegetables; the using amount of pesticides is reduced, the residual amount of the pesticides on crops is reduced, and the environmental pollution is reduced; and the insecticidal composition is safe to people and livestock, has high environmental compatibility, and ensures that pests cannot produce the pesticide resistance easily.

The invention belongs to the field of the seed pelleting technology, specifically relates to the pelleting method of brassicaceous vegetable seeds. The invention provides a utilization method of plant pesticides in the vegetable seed pelletization, and Azadirachtin, Matrine, Celangulin are the active ingredients. The pelleting seeds using the method have the merits of high germination percentage, low toxicity and good environmental compatibility, and can effectively prevent seed-borne disease of brassicaceous vegetable seeds and reduce the harm of soil insects.

The invention relates to a pesticide composition which is characterized by comprising the effective components of tolfenpyrad and butene-fipronil or ethiprole. The pesticide composition has excellent control effect on flea beetles which are harmful to Cruciferae plants and also has good control effect on other injurious insects, such as aphids, diamond back moths, cabbage caterpillars, and the like on the Cruciferae plants.

The invention provides a rooting agent for preventing and treating clubroot diseases of cruciferae crops. Effective constituents of the rooting agent comprise 0.1-0.5 part of a water-soluble boron compound, 140-250 parts of a water-soluble nitrogen compound and 49-60 parts of organic fertilizer. The rooting agent is applied in the growth period after plants, infected with the clubroot diseases, of the cruciferae crops such as oilseed rape come into twelve true leaves, induction and growth of enough new roots of the plants, infected with the clubroot diseases, of the cruciferae crops such as the oilseed rape can be effectively facilitated, the yield loss of the plants infected with the diseases is lowered to the greatest extent, and economic benefits of growers are guaranteed. The invention further provides a preparing method of the rooting agent, wherein the rooting agent is prepared by mixing raw materials and then conducting granulation, operation is easy and convenient, and cost is low.

The invention relates to a compound chemical hybridizing agent composite and application thereof. The composite contains compound tribenuron-methyl, thifensulfuron methyl, a boricfertilizer, a dispersing agent, soluble starch and the like. The composite can induce the male sterility and female fertility of crucifer. In comparison with a single agent, the compound chemical hybridizing agent has a good and thorough male sterility effect, a male sterility rate of over 95 percent and a female fertility rate of 100 percent. The compound chemical hybridizing agent has bioactivity which is 100 to 1,000 times as high as that of the traditional hybridizing agent, the used amount is 0.15 to 0.3g/hm<2>, and the compound chemical hybridizing agent has the advantages of superhigh efficiency, super-low used amount, low toxicity, low residue and low cost.

The invention provides a fermentation method of bacillus amyloliquefaciens Bam22 for preventing and treating cruciferae plasmodiophora brassicae. The method comprises the following steps: (1) picking up a bacillus amyloliquefaciens Bam22 colony cultivated on a solid culture medium, and carrying out shake culture in a liquid culture medium at 30-32 DEG C for 10-12 hours, wherein the rotating speed is 200-220rpm; and the product is taken as a seed solution; (2) fermenting the seed solution obtained in the step (1) in a fermentation tank for 40-50 hours, wherein the access amount of the seed solution is 8%-10%; the temperature of the tank is 27-30 DEG C; the stirring speed of the fermentation tank is 220-250rpm; the air flow is 0.8:1 to 1:1 (V/V.m); the efficient bacillus amyloliquefaciens Bam22 for cruciferae plasmodiophora brassicae is preserved at the General Microbiology Center of the China Committee for Culture Collection of Microorganisms on September 6, 2015; and the preservation number is CGMCC No.11329. The fermentation method provided by the invention is very favorable to cultivation of the bacillus amyloliquefaciens Bam22; and multiple bacteria are obtained and have good activity.