The invention discloses a low-temperature preservation process of Taxus chinensis var. The low-temperature preservation process includes the basic steps that a superior tree is selected for collectingseeds, arils of the collected seeds are washed, then the seeds are exposed to the sun for 2-3 days, stored in a bag for storage, the temperature is 4-5 DEG C, the seeds are place for 5-7 months, after natural temperature recovery, the dormancy is broken, after mechanically removing of hulls is carried out, soaking is carried out in distilled water for 24 hours, changing of water is carried out for several times until the inner coats of the seeds are removed, then the processed seeds are placed under sterile conditions and placed in 1% potassium permanganate solution for soaking and disinfection for 30 minutes, then rinsing with sterile water is carried out, the processed seeds are transferred to a freeze pipe, after 10% DMSO cryoprotectant is added, the freeze pipe is sealed and then placed in an ice bath, soaking treatment is carried out under 250W ultrasonic conditions for 10-15 minutes, then the processed pipe is put in a cold storage at -16-(-18) DEG C to pre-froze for 75min, andthen the processed pipe is put in liquid nitrogen for freezer storage after treatment is completed. Compared with traditional seed preservation methods, the process can further enhance seed vigor, andgreatly increase the amount of TTC reduction, the preservation time is longer, the cost is lower, and the economic and social benefits are better.