87 results about "Urechis unicinctus" patented technology

The application relates to a composite flavoring mainly comprising urechis unicinctus powder and kelp powder as main materials and a preparation method of the composite flavoring. Proteolytic enzyme is adopted to hydrolyze urechis unicinctus into micro-molecular amino acid and peptide which are easier to be absorbed by a human body; moreover, laminaria japonica is processed by adopting methods of boiling and adding vinegar for immersing, as a result, the fishy smell of the laminaria japonica can be effectively removed, and the aborption and utilization of the effective ingredients of the laminaria japonica are facilitated. The urechis unicinctus is rich in proteins, microelements and essential amino acid for the human body, the laminaria japonica is rich in vitamins and mineral substances, and the combination of the urechis unicinctus and the laminaria japonica not only has a fresh, delicious and unique flavor, but also is reasonable in the matching of nutrient ingredients, and can well satisfy the demand of the human body for the nutrient ingredients. All the raw materials in the flavoring provided by the invention are from natural substances, so that the composite urechis unicinctus flavoring is healthier and safer.

The invention discloses a preparation method of an urechis unicinctus visceral protein source zinc chelating peptide. The zinc chelating peptide takes utrechis unicinctus viscera as a raw material, and the product is obtained through viscera pretreatment, protein extraction, enzyme hydrolysis, gel purification and chromatography. The prepared zinc chelating peptide has an amino acid sequence of Phe-Pro-Tyr-Lys-His, and the ESI-MS detection molecular weight is 690.78 Da. The preparation of the zinc chelating peptide provides a technical support for development of zinc supplemental health-care food and drugs, and also provides a new idea for high-valued utilization of the urechis unicinctus viscera.

The invention relates to an HPLC (high performance liquid chromatography) and GPC (gel permeation chromatography) based separation and purification method for marine organism sourced polypeptide. According to the method, urechis unicinctus viscera are selected and crushed, and urechis unicinctus visceral meal is prepared; the urechis unicinctus visceral meal is subjected to enzymatic hydrolysis after concentration; a product of the urechis unicinctus visceral meal subjected to enzymatic hydrolysis is concentrated, frozen and dried and then is subjected to Sephadex G-15 gel chromatography and HPLC separation and purification, and Gln-Pro-Met-Thr-Phe is obtained. The method has the advantages that an extraction and purification technology is more advanced, extraction and purification are fast and high in accuracy, and ACE (angiotensin I-converting enzyme) inhibition activity of the prepared polypeptide is remarkable.

The invention discloses a method for preparing urechis unicinctus glycosaminoglycan and antibacterial peptide simultaneously. The method includes: raw material process, producing raw material of urechis unicinctus into a homogenate; enzymolysis; ultrafitration; preparation of glycosaminoglycan, eliminating protein, ethanol precipitation and washing, anion exchange column chromatography, gel filtration chromatography to obtain glycosaminoglycan with high purity; and preparation of the antibacterial peptide. According to the method, the reaction condition is mild, the preparation process is environment-friendly and energy-saving, and the added value of products is high; the purity of the prepared glycosaminoglycan and the antibacterial peptide is high; a large amount of protein can be recycled, the comprehensive utilization of the urechis unicinctus is facilitated, the prepared glycosaminoglycan has obvious effects of anticoagulant and free radical scavenging, and the prepared antibacterial peptide has obvious bacteriostatic activity to staphylococcus aureus, Escherichia coli, Aeromonas hydrophila and the like; and the obtained glycosaminoglycan and the urechis unicinctus can provide fine immunopotentiators and novel antibacterial agents for marine rare life aquaculture.

The invention discloses a condiment. The condiment is characterized in that: the condiment is prepared by hydrolyzing urechis unicinctus (nereis succinea) serving as a raw material through protease, mixing inactivated enzyme hydrolysis liquid with auxiliary materials and drying the mixture. The condiment of the invention contains amino acid and small peptide which can be easily digested and absorbed by a human body instead of the high protein of the urechis unicinctus (nereis succinea). A contrast test shows that the food made by the condiment of the invention has a better taste.

The invention discloses a culture medium and method for culturing a urechis unicinctus trochophore cell line. The culture medium is prepared by adding 20.2g / l of NaCl, 0.54g / l of KCl, 0.60g / l of CaCl2, 1g / l of MgSO4, 3.9g / l of MgCl2, 2.92g / l of L-glutamine, 5% of fetal bovine serum, 2% of urechis unicinctus coelomic fluid, 1% of urechis unicinctus yolk extract, penicillin with final contraction being 100IU / ml and streptomycin with final concentration being 100 microgram / ml into L-15 culture medium dry powder. The culture method is characterized in that the culture medium is used to perform trochophore single-cell primary culture, early passage is achieved, and subculturing is performed. The culture medium is high in specificity, can provide comprehensive nutrition to the cell growth and proliferation of the cell line and has great reference value to the building of other marine invertebrate trochophore cell lines.

The invention relates to an active polypeptide prepared by high performance liquid chromatography and application thereof, belonging to the technical field of marine organisms. The new short peptide containing 5 amino acid sequences is separated and purified from the protein enzymolysis product of the Urechis unicinctus viscera, the sequence is identified to be Gln-Pro-Met-Thr-Phe, and the ESI-MS detection molecular weight is 552.72 Da. The in-vitro activity indicates that the active polypeptide has favorable angiotensin converting enzyme (ACE) inhibition activity and can be used for preparing anti-hypertension health food.

The invention belongs to the field of aquatic livestock breeding, and particularly relates to an indoor breeding device and method for urechis unicinctus. The breeding device comprises a breeding pond, breeding boxes, bricks, microporous inflation pipes and a water overflow pipe. The bricks are arranged at the bottom of the breeding pond in a matrix shape, each microporous inflation pipe is laid between the two adjacent rows of bricks, the breeding boxes with a plurality of round holes are placed on the bricks, net boxes, grait and screen meshes are sequentially laid on the bottoms of the boxes, and the water overflow pipe is arranged at the bottom of the breeding pond. The breeding method comprises the steps that 1, fry are bred; 2 the fry are fed; 3, in the breeding stage, feeding, water change and temperature controlling are carried out; 4, box shifting and fry screening are carried out; 5, the fry are developed. The bricks are adopted as supporting bodies, the breeding boxes are adopted as carriers, inflation equipment is laid on the bottom of the pond, organic matter in the breeding device is discharged out, local pollution is avoided, the living environment of the urechis unicinctus is improved, the indoor breeding sand changing and pond shifting time is prolonged, the breeding yield is improved, and labor cost is lowered.

The invention relates to a preparation method for urechis unicinctus seasoning liquid. The method comprises the following steps: taking fresh urechis unicinctus as a raw material, conventionally processing, and grinding to obtain urechis unicinctus pulp; adding compound protease into the urechis unicinctus pulp for controlled enzymolysis to obtain enzymolysis liquid; inactivating the enzymolysis liquid, filtering, adding 1-5% of pure active carbon and 0.5-1.5% of beta-cyclodextrine based on the weight of the enzymolysis liquid for debittering, and filtering to obtain urechis unicinctus refined liquid; concentrating the refined liquid, adding 0.05-0.5% of a colorant, 0.05-0.5% of an emulsifier and 5-25% of a seasoner based on the weight of concentrated liquid for seasoning and homogenizing, degassing in vacuum, filling, sealing, and sterilizing to obtain the finished urechis unicinctus seasoning liquid. The method is reasonable in technology, mild in process and free of environmental pollution; the prepared urechis unicinctus seasoning liquid keeps all the nutritive ingredients and flavor factors of the urechis unicinctus, is thick in seafood flavor and rich in nutrition, has the healthcare effects of warming the liver and kidney, tonifying yang, consolidating essence and the like, and can greatly meet the seasoning and nutritional requirements of people on seasonings.

The invention discloses parent Navodon septentrionalis reproduction maturing promoting bait and a preparation method thereof. The parent Navodon septentrionalis reproduction maturing promoting bait comprises the following components in percentage: 8 to 13 percent of clam worm, 8 to 13 percent of oyster meat, 8 to 13 percent of mussel meat, 25 to 35 percent of artificial compound feed powder, 0.2 to 0.4 percent of vitamin C, 0.1 to 0.2 percent of vitamin E, 0.6 to 0.8 percent of immune polysaccharide, 0.2 to 0.4 percent of Coptis chinensis, Scutellaria baicalensis and golden cypress powder or rheum officinale Coptis chinensis, Scutellaria baicalensis and golden cypress powder, 0.2 to 0.4 percent of spirulina powder, 0.2 to 0.4 percent of lecithin, 8 to 13 percent of Trachypenaeus curvirostris, 8 to 13 percent of leftovers from pectinid cylinder processing and 5 to 10 percent of residual internal organs from urechis unicinctus processing. The bait can satisfy the nutrient demands of parent fish, improve the developmental rate of sexual gland of parent fish, promote the synchronous development of sexual gland, improve egg laying amount and ovum quality of parent fish and lay a good foundation for the scale seedling breeding of Navodon septentrionalis.

The invention discloses an urechis unicinctus farming method. By applying a micropore oxygenation technology to an urechis unicinctus pond and in cooperation with the selenium-added feed, the growth effect of urechis unicinctus living on the bottom layer is promoted, the yield of urechis unicinctus is increased, and selenium-rich urechis unicinctus with rich nutrition is produced. The selenium-added feed is prepared from eucheuma gel extraction residue extract, degummed kelp powder, sargassum powder, scallop meat powder, shrimp shell powder, medical stone powder, squid organ meal, shell powder, sweet potato leaves powder, bile acid and selenium methionine.

The invention belongs to the technical field of biological active peptide. The invention discloses urechis unicinctus biological active peptide, wherein the amino acid sequence is Thr-Arg-Gly-Pro-Pro.The invention also discloses a preparation method of the urechis unicinctus biological active peptide, which is a method for extracting the urechis unicinctus biological active peptide from the bodywall of the urechis unicinctus. The urechis unicinctus biological active peptide has pressure-reducing activity, has high angiotensin converting enzyme (ACE) inhibition activity and can inhibit proliferation of vascular endothelial cells.

This invention discloses a method for manufacturing a fibrinolytic enzyme from whole-body or coelomic fluid of Urechis unicinctus, which comprises the steps of: (1) grinding and / or separating the tissues, (2) centrifuging to obtain the clear supernatant, (3) ultrafiltrating, (4) freeze-drying to obtain freeze-dried powders, or precipitating with acetone at a low temperature and vacuum filtrating to obtain the precipitate, (5) dissolving, purifying through a gel permeation column, and freeze-drying to obtain the fibrinolytic enzyme. The enzyme has good anticoagulation effects, and can be used to manufacture drugs for treating cardiovascular and cerebrovascular diseases.

The present invention relates to compound urechis unicinctus seasoning materials and a preparation method and an application thereof. The recipe of the compound urechis unicinctus seasoning materials is as follows: 90% of urechis unicinctus seasoning raw liquid, 2% of edible salt, 4% of disodium 5'-ribonucleotide and 4% of an emulsifier. The seasoning materials can be made into microcapsules. The preparation method of the urechis unicinctus seasoning raw liquid is as follows: fresh urechis unicinctus is selected as raw materials, heads, tails and internal organs of the urechis unicinctus are removed, the treated urechis unicinctus is washed clean using tap water, the washed urechis unicinctus is cut into segments, water with amount being one time of the weight of the urechis unicinctus is added, and the urechis unicinctus segments are minced and beat with a beating machine to obtain urechis unicinctus pulp; then the urechis unicinctus pulp is boiled under normal pressure, and the boiled urechis unicinctus pulp is filtered to obtain urechis unicinctus meat residues and urechis unicinctus soup juice; water with amount being 5-10 times of the urechis unicinctus meat residues is added into the urechis unicinctus meat residues, the mixture is stirred evenly, pH value of the mixture is adjusted, alkaline protease and flavoring protease are added, the mixture is stirred, the stirred mixture is enzymolyzed, the enzymolyzed material is then heated to 90-100 DEG C, the heated material is subjected to enzyme deactivation, the enzyme deactivated material is cooled to room temperature, and the cooled material is filtered to obtain urechis unicinctus enzymatic hydrolysate; and the urechis unicinctus enzymatic hydrolysate is mixed with urechis unicinctus soup juice at a volume ratio of 1:(5-8) evenly to obtain urechis unicinctus seasoning raw liquid. Obviously, the compound urechis unicinctus seasoning materials fully retain the original juice and original taste of the urechis unicinctus, and are rich in nutrition, significant in freshness increasing, and natural and healthy composite seafood seasonings.

The present invention relates to a squid stuffing dumpling, which comprises dumpling wrappers and a stuffing, wherein the stuffing comprises, by mass, 15-25 parts of squid, 5-15 parts of seaweed, 12-20 parts of seafood mud, 1-3 parts of a seasoning and 2-5 arts of a composite soup paste. According to the present invention, the product is green, health and novel, is easy to eat, and meets requirements of consumers; the product is rich in iodine, selenium and other trace elements and a variety of amino acids, such that effects of body constitution enhancing, immunity improving, aging delaying and the like can be provided after long-term consumption of the product; cortex mori radicis, salvia miltiorrhiza bunge, tuckahoe and licorice root are added to the stuffing so as to provide effects of liver protection, intestine and stomach invigorating, antibacterial and blood fat lowering; and the seafood mud comprising nereis succinea, urechis unicinctus and shrimp mud is added to combine with the and squid meat so as to make the seafood flavor significant, and the nereis succinea and the urechis unicinctus are rich in nutrition so as to meet the health consumption concept.

The invention belongs to the technical field of biological products, and particularly relates to a preparation method and application of urechis unicinctus autolytic enzyme. The preparation method comprises the steps of taking urechis unicinctus internal organs, performing cleaning with bacteria-free water, performing homogenization, adding PBS, performing leaching, performing loading in low-temperature environment, performing standing overnight, then performing centrifugation, and collecting supernatant; performing gradient precipitating in low-temperature environment with a saturated ammonium sulfate solution to obtain protein precipitate, performing resolvation with PBS buffer liquid, and performing dialysis to obtain an autolytic enzyme crude enzyme solution; and performing column chromatography and separation and purification on the crude enzyme solution to obtain the urechis unicinctus autolytic enzyme. The urechis unicinctus autolytic enzyme prepared by the method has favorablethermal stability, has favorable wall-breaking effects on algal cells of chlorella, and can be applied to extraction of active substances in aquatic animals and processing by-products, and productionand processing of foods including algae, so as to improve degradation effects and reduce production cost.

The invention discloses a breeding method for shortening float periods of urechis unicinctus larvae. The breeding method particularly includes feeding isochrysis 3011 to the urechis unicinctus larvae from the third day to the seventh day after the urechis unicinctus larvae are fertilized, and keeping the temperatures at 15.5-16 DEG C; keeping the water temperatures at 16.5-17 DEG C from the seventh day to the twelfth day; keeping the water temperatures at 17.5-18 DEG C from the thirteenth day to nineteenth day and changing 1 / 2 volumes of water twice on a daily basis; feeding feed four times on a daily basis from the seventh day to nineteenth day. The isochrysis 3011 is initial feed, and the feeding quantities of the initial feed is 40ml / m<3>d. Formulas and usage quantities of the feed in different procedures from the seventh day to nineteenth day are different from one another. The breeding method has the advantages that the metamorphosis and settlement time of the urechis unicinctus larvae in the float periods can be shortened, breeding periods can be shortened by 15-25 days as compared with schemes in the prior art, and the breeding cost can be reduced to a great extent.

The invention relates to a preparation method of urechis unicinctus viscera glycosaminoglycan. The method comprises the following steps: using urechis unicinctus viscera, rubbing and homogenizing, degreasing and performing pumping filtration to obtain the urechis unicinctus viscera dry powder, then hydrolyzing the viscera dry powder by papain and trypsin, extracting a viscera glycosaminoglycan crude product from a hydrolysate, and performing dialysis and freeze drying to obtain the viscera glycosaminoglycan. The invention also relates to an application of the viscera glycosaminoglycan in preparation of food, health product and medicine for preventing and adjusting blood fat. The extraction method is simple, yield can reach 6-7%, protein content of viscera glycosaminoglycan can be greatly reduced, purity of glycosaminoglycan is increased, and the method provides a novel approach for preventing and adjusting blood fat.

The total RNA of Urechis unicinctus, which is used as template, is cloned to obtain the full-length cDNA sequence of the Urechis unicinctus fibrinolysin gene, wherein the open reading frame (ORF) of the sequence has 792 basic bases and is from the initiation codon ATG to the stop codon TAA. The cDNA original coding sequence of the fibrinolysin gene is translated to obtain the amino acid sequence of the Urechis unicinctus fibrinolysin, wherein the amino acid sequence has 263 amino acids in all. Different recombinant plasmid vectors can be constructed to construct fibrinolysin gene engineering bacteria for different receptors such as Escherichia coli and Saccharomyces and ensure that the cDNA sequence of the Urechis unicinctus fibrinolysin gene is expressed in the gene engineering bacteria and prepare fibrinolysin. The prepared fibrinolysin can be utilized to prepare the anticoagulant medicines or antithrombotic medicines for curing cardiovascular diseases and have very good research and development prospects.

The invention relates to a preparation technology of an interventional thrombolytic biological product namely urechis unicinctus plasmin. The technology comprises the following steps: freezing coelomic fluid of urechis unicinctus, then subjecting the fluid to centrifugation and suction filtration, carrying out freeze drying in vacuum to obtain a coarse product, subjecting the coarse product to DEAE (diethyl amino ethanol)-Sepahcel (cellulose glucan gel) anion exchange chromatography, Sephadex (hydroxypropyl glucan gel)G-75 chromatography, benzamidine-Sepharose (agarose gel) chromatography, and freeze drying to further verify that the coarse product is plasmin, and then carrying out an interventional thrombolytic experiment on a rat carotid and cerebral artery embolism model to prove that the urechis unicinctus plasmin can completely dissolve thrombus and does not have any side effect such as allergy, and the like.

The invention discloses a method for processing urechis unicinctus dumplings and comprehensively utilizing waste of the dumplings. The method comprises the following steps: processing urechis unicinctus: processing the urechis unicinctus fished on the sea in time while keeping the urechis unicinctus fresh, cutting off two ends of the urechis unicinctus, removing impurities, quickly freezing the urechis unicinctus at -40 to -30 DEG C, and conducting storage; processing leek: removing rotten leaves and soil, retaining leek shoots, and removing leek leaves; manufacturing dumplings; treating urechis unicinctus waste: subjecting the urechis unicinctus waste to dehydration and quick-freezing, and preparing a feed for mariculture; and treating leek waste: subjecting the leek waste to dehydration,and preparing a feed for breeding livestock and poultry. The urechis unicinctus is processed by the method of the invention, so that the quality and taste of the urechis unicinctus are ensured, nutrient loss and nutrient deterioration are avoided, the processed dumplings are delicious in taste, fresh, and good in mouth feel, and the limitation that leek urechis unicinctus dumplings prepared by traditional methods can only be prepared when needed is broken.

The invention discloses an urechis unicinctus collecting device. The device comprises an urechis unicinctus collecting net box (11) with an opening on a bottom surface, a net pocket arranged (9) on one side surface and remaining surfaces covered with mesh cloth, first main pipes (1) are transversely arranged at the lower portions of the opposite side surfaces of the urechis unicinctus collecting net box (11) and the net pocket (9), third main pipes (3) in communication with the first main pipes (1) through first connecting pipes (12) are disposed at the lower portions of the first main pipes (1), and second main pipes (2) disposed in parallel with the third main pipes (3) are communicated with the third main pipes (3) through second connecting pipes (13); a water spraying pipe sprays out the water to wash a sand layer at the bottom of a pool, the impulse is used for making the urechis unicinctus rush out and float upward, and water flows the floating urechis unicinctussea into the netpocket at the back of the collecting device for collection, which reduces the cost, improves collection efficiency, and reduces the rate of urechis unicinctus residual compared with manual dredging.

The invention relates to a compound traditional Chinese medicine formula for treating cardio-cerebral vessel embolism and belongs to the technical field of traditional Chinese medicine formulas.The compound traditional Chinese medicine formula for treating cardio-cerebral vessel embolism comprises, by mass, 4-6 g of tempeh extract, 8-12 g of sea cucumber extract, 4-6 g of hirudo extract, 3-8 g of Urechis unicinctus extract, 3-8 g of Radix Salviae Miltiorrhizae extract, 8-13 g of Radix Astragali extract, 90-110 mg of coenzyme Q10, 180-220 mg of procyanidine, 70-110 mg of sodium selenite, 1800-2200 IU of vitamin A, 28-32 mg of vitamin B2, 25-35 mg of folic acid, 5-7 g of lecithin and 800-1100 mg of carnitine.The compound traditional Chinese medicine formula for treating cardio-cerebral vessel embolism is reasonable in design, good in treating effect by combining traditional Chinese medicines with dietetic invigoration, low in price and free of side effect.

The invention discloses a Urechis unicinctus feed. The feed is prepared by using the following raw materials, by weight, 25-40 parts of degummed kelp powder, 10-20 parts of gulfweed powder, 8-12 parts of edible seaweed powder, 5-8 parts of soybean protein powder, 8-15 parts of scallop meat powder, 5-10 parts of shrimp shell powder, 5-10 parts of corn germ powder, 1-3 parts of bile acid, 3-6 parts of fish meal, 0.5-1.5 parts of pillared montmorillonite powder, 0.5-0.8 parts of composite vitamin and 0.3-0.5 parts of composite trace element. The Urechis unicinctus feed has the advantages of balanced and comprehensive nutrition and good palatability, allows Urechis unicinctus fed with the feed to have the advantags of good growth, fast growth speed and high weight gain, and benefits the large area popularization culture of the Urechis unicinctus.

The present invention relates to a nutrition hot pot seafood seasoning material, and belongs to the technical field of food seasoning materials. The nutrition hot pot seafood seasoning material is characterized by comprising the following traditional Chinese medicine raw material components by weight: 20-30 parts of chicken bone powder, 30-50 parts of shrimp powder, 10-20 parts of crab powder, 30-40 parts of oyster powder, 10-30 parts of squid powder, 5-10 parts of ginseng powder, 2-6 parts of ginger powder, 10-15 parts of allium fistulosum powder, 3-10 parts of garlic powder, 20-50 parts of urechis unicinctus powder, 10-15 parts of maltodextrin, 5-10 parts of onion powder, 10-30 parts of glossogyne tenuifolia cass powder, 10-20 parts of black sesame powder, 1-5 parts of white pepper powder, 5-20 parts of red date powder, 5-10 parts of Chinese wolfberry powder, 5-15 parts of trehalose, 10-20 parts of papaya powder, 3-8 parts of pericarpium citri reticulatae powder, and 5-10 parts of angelica sinensis powder. According to the present invention, the nutrition hot pot seafood seasoning material of the present invention has characteristics of delicious taste and rich nutrition, the powder is easy to carry and transport, the raw materials are derived from the natural food materials and the traditional Chinese medicine components, and the eating is safe.

The invention belongs to the field of aquatic livestock breeding, and particularly relates to a device and method for collecting urechis unicinctus fry. According to the collecting device, one end of a water inlet pipe is connected with a hose through a valve, and the other end of the water inlet pipe is vertically communicated with the side wall of a main pipe; a transverse branch pipe is vertically communicated with one end of the main pipe, at least one transverse nozzle is vertically arranged on the side wall of the transverse branch pipe, at least one inclined branch pipe is arranged on the side wall of the main pipe, and the top of each inclined branch pipe is provided with an inclined nozzle. The fry are collected through the collecting device, and the method comprises the steps that 1, water is drained for the first time; 2, wand flushing is carried out, and water is drained for the second time; 3, flow speed is adjusted at the same time in the step 2; 4, the fry are collected. The sand flushing method is adopted, the urechis unicinctus fry inhabiting in a sand bed can be collected better and faster, the influence of blackening and smelliness of the sand bed on growth of the urechis unicinctus fry is reduced, the fry collecting speed is improved by two to three times, and the damage rate of the urechis unicinctus fry is reduced.

The invention relates to urechis unicinctus sperm cryopreservation liquid which comprises urechis unicinctus sperms, preservatives and antifreeze agents. A preparation method of the cryopreservation liquid includes the steps: collecting sperms; identifying sperm activity; diluting the sperms; reducing temperature, and balancing; firstly pre-cooling; secondly pre-cooling; preserving; unfreezing; eluting and the like. According to the cryopreservation liquid, the urechis unicinctus sperms can be effectively preserved for a long time and are preserved in liquid nitrogen, and activation rate of unfrozen sperms can reach 70% or more. According to the preparation method, expensive instruments are omitted, and the preparation method is simple to operate, low in cost and good in effect, provides sperm source guarantee for artificial insemination operations of urechis unicinctus and can be used for other research fields such as crossbreeding, germplasm selection, gynogenesis and species protection of the urechis unicinctus.

The invention discloses a method for stereo ecological aquaculture of urechis unicinctus. The method includes steps of pond repairing, water quality control, fry releasing, bait casting, disease prevention and control, harvesting and the like. By adoption of the method for stereo ecological aquaculture of urechis unicinctus and other organisms, the aquaculture water space and baits can be utilizedcompletely, the aquaculture capacity and unit water yield are increased, economic benefits of mariculture in coastal regions are increased, and important economic significance and social significanceare achieved.

The invention discloses an artificial compound feed capable of promoting growth of blood and blood cells of bodies of urechis unicinctus. The artificial compound feed is characterized by being prepared from the following raw materials in parts by mass of 10-20 parts of soybean flour, 10-20 parts of kelp powder, 20-35 parts of gulfweed powder or sargassum thunbergiio.kuntze powder, 10-20 parts of fish meal, 10-20 parts of clamworm powder, 3-5 parts of blood powder, 5-10 parts of oyster shell powder, 0.5-1 part of complex vitamins, 0.2-0.5 part of composite trace elements, 0.5-1 part of composite traditional Chinese medicine powder and 0.5-1 part of a compound iron preparation. The feed is particulate and the particle size range is 80-150 meshes.