87 results about "Urechis unicinctus" patented technology

The invention discloses a preparation method of an urechis unicinctus visceral protein source zinc chelating peptide. The zinc chelating peptide takes utrechis unicinctus viscera as a raw material, and the product is obtained through viscera pretreatment, protein extraction, enzyme hydrolysis, gel purification and chromatography. The prepared zinc chelating peptide has an amino acid sequence of Phe-Pro-Tyr-Lys-His, and the ESI-MS detection molecular weight is 690.78 Da. The preparation of the zinc chelating peptide provides a technical support for development of zinc supplemental health-care food and drugs, and also provides a new idea for high-valued utilization of the urechis unicinctus viscera.

The invention relates to an HPLC (high performance liquid chromatography) and GPC (gel permeation chromatography) based separation and purification method for marine organism sourced polypeptide. According to the method, urechis unicinctus viscera are selected and crushed, and urechis unicinctus visceral meal is prepared; the urechis unicinctus visceral meal is subjected to enzymatic hydrolysis after concentration; a product of the urechis unicinctus visceral meal subjected to enzymatic hydrolysis is concentrated, frozen and dried and then is subjected to Sephadex G-15 gel chromatography and HPLC separation and purification, and Gln-Pro-Met-Thr-Phe is obtained. The method has the advantages that an extraction and purification technology is more advanced, extraction and purification are fast and high in accuracy, and ACE (angiotensin I-converting enzyme) inhibition activity of the prepared polypeptide is remarkable.

The invention discloses an artificial urechis unicinctus fry and fingerling culture method. Fry and fingerling culture is started in autumn. The method includes the steps: S1 urechis unicinctus parentselection and temporary culture; S2 artificial spawning and natural insemination; S3 incubation and larva selection; S4 daily management of planktonic larvae; S5 young artificial urechis over-wintering culture. According to the artificial urechis unicinctus fry and fingerling culture method, the water temperature of a natural sea area is reduced, water quality is stable, diseases are not easily caused, an idle fry and fingerling culture workshop in winter is sufficiently used, water changing quantity is small, heating is omitted, production cost is reduced, fries and fingerlings can be discharged from a pond in the middle ten days of April after indoor over-wintering growth for 4-5 months in winter, fry and fingerling discharging time is 2 months shorter than that of fry and fingerling culture in spring, fry and fingerling specifications reach about 5cm and are far larger than those of the fries and fingerlings cultured in spring, survival rate is increased by 30% or more, and early fries and fingerlings and large fries and fingerlings are discharged. Farmers perform culture for 5 months, and the fries and fingerlings can be successively harvested and appear on the market at the end of September in the current year.

The invention discloses a culture medium and method for culturing a urechis unicinctus trochophore cell line. The culture medium is prepared by adding 20.2g/l of NaCl, 0.54g/l of KCl, 0.60g/l of CaCl2, 1g/l of MgSO4, 3.9g/l of MgCl2, 2.92g/l of L-glutamine, 5% of fetal bovine serum, 2% of urechis unicinctus coelomic fluid, 1% of urechis unicinctus yolk extract, penicillin with final contraction being 100IU/ml and streptomycin with final concentration being 100 microgram/ml into L-15 culture medium dry powder. The culture method is characterized in that the culture medium is used to perform trochophore single-cell primary culture, early passage is achieved, and subculturing is performed. The culture medium is high in specificity, can provide comprehensive nutrition to the cell growth and proliferation of the cell line and has great reference value to the building of other marine invertebrate trochophore cell lines.

The invention belongs to the field of aquatic livestock breeding, and particularly relates to an indoor breeding device and method for urechis unicinctus. The breeding device comprises a breeding pond, breeding boxes, bricks, microporous inflation pipes and a water overflow pipe. The bricks are arranged at the bottom of the breeding pond in a matrix shape, each microporous inflation pipe is laid between the two adjacent rows of bricks, the breeding boxes with a plurality of round holes are placed on the bricks, net boxes, grait and screen meshes are sequentially laid on the bottoms of the boxes, and the water overflow pipe is arranged at the bottom of the breeding pond. The breeding method comprises the steps that 1, fry are bred; 2 the fry are fed; 3, in the breeding stage, feeding, water change and temperature controlling are carried out; 4, box shifting and fry screening are carried out; 5, the fry are developed. The bricks are adopted as supporting bodies, the breeding boxes are adopted as carriers, inflation equipment is laid on the bottom of the pond, organic matter in the breeding device is discharged out, local pollution is avoided, the living environment of the urechis unicinctus is improved, the indoor breeding sand changing and pond shifting time is prolonged, the breeding yield is improved, and labor cost is lowered.

The invention discloses parent Navodon septentrionalis reproduction maturing promoting bait and a preparation method thereof. The parent Navodon septentrionalis reproduction maturing promoting bait comprises the following components in percentage: 8 to 13 percent of clam worm, 8 to 13 percent of oyster meat, 8 to 13 percent of mussel meat, 25 to 35 percent of artificial compound feed powder, 0.2 to 0.4 percent of vitamin C, 0.1 to 0.2 percent of vitamin E, 0.6 to 0.8 percent of immune polysaccharide, 0.2 to 0.4 percent of Coptis chinensis, Scutellaria baicalensis and golden cypress powder or rheum officinale Coptis chinensis, Scutellaria baicalensis and golden cypress powder, 0.2 to 0.4 percent of spirulina powder, 0.2 to 0.4 percent of lecithin, 8 to 13 percent of Trachypenaeus curvirostris, 8 to 13 percent of leftovers from pectinid cylinder processing and 5 to 10 percent of residual internal organs from urechis unicinctus processing. The bait can satisfy the nutrient demands of parent fish, improve the developmental rate of sexual gland of parent fish, promote the synchronous development of sexual gland, improve egg laying amount and ovum quality of parent fish and lay a good foundation for the scale seedling breeding of Navodon septentrionalis.

The invention discloses an urechis unicinctus farming method. By applying a micropore oxygenation technology to an urechis unicinctus pond and in cooperation with the selenium-added feed, the growth effect of urechis unicinctus living on the bottom layer is promoted, the yield of urechis unicinctus is increased, and selenium-rich urechis unicinctus with rich nutrition is produced. The selenium-added feed is prepared from eucheuma gel extraction residue extract, degummed kelp powder, sargassum powder, scallop meat powder, shrimp shell powder, medical stone powder, squid organ meal, shell powder, sweet potato leaves powder, bile acid and selenium methionine.

The invention belongs to the technical field of biological active peptide. The invention discloses urechis unicinctus biological active peptide, wherein the amino acid sequence is Thr-Arg-Gly-Pro-Pro.The invention also discloses a preparation method of the urechis unicinctus biological active peptide, which is a method for extracting the urechis unicinctus biological active peptide from the bodywall of the urechis unicinctus. The urechis unicinctus biological active peptide has pressure-reducing activity, has high angiotensin converting enzyme (ACE) inhibition activity and can inhibit proliferation of vascular endothelial cells.

This invention discloses a method for manufacturing a fibrinolytic enzyme from whole-body or coelomic fluid of Urechis unicinctus, which comprises the steps of: (1) grinding and/or separating the tissues, (2) centrifuging to obtain the clear supernatant, (3) ultrafiltrating, (4) freeze-drying to obtain freeze-dried powders, or precipitating with acetone at a low temperature and vacuum filtrating to obtain the precipitate, (5) dissolving, purifying through a gel permeation column, and freeze-drying to obtain the fibrinolytic enzyme. The enzyme has good anticoagulation effects, and can be used to manufacture drugs for treating cardiovascular and cerebrovascular diseases.

The present invention relates to compound urechis unicinctus seasoning materials and a preparation method and an application thereof. The recipe of the compound urechis unicinctus seasoning materials is as follows: 90% of urechis unicinctus seasoning raw liquid, 2% of edible salt, 4% of disodium 5'-ribonucleotide and 4% of an emulsifier. The seasoning materials can be made into microcapsules. The preparation method of the urechis unicinctus seasoning raw liquid is as follows: fresh urechis unicinctus is selected as raw materials, heads, tails and internal organs of the urechis unicinctus are removed, the treated urechis unicinctus is washed clean using tap water, the washed urechis unicinctus is cut into segments, water with amount being one time of the weight of the urechis unicinctus is added, and the urechis unicinctus segments are minced and beat with a beating machine to obtain urechis unicinctus pulp; then the urechis unicinctus pulp is boiled under normal pressure, and the boiled urechis unicinctus pulp is filtered to obtain urechis unicinctus meat residues and urechis unicinctus soup juice; water with amount being 5-10 times of the urechis unicinctus meat residues is added into the urechis unicinctus meat residues, the mixture is stirred evenly, pH value of the mixture is adjusted, alkaline protease and flavoring protease are added, the mixture is stirred, the stirred mixture is enzymolyzed, the enzymolyzed material is then heated to 90-100 DEG C, the heated material is subjected to enzyme deactivation, the enzyme deactivated material is cooled to room temperature, and the cooled material is filtered to obtain urechis unicinctus enzymatic hydrolysate; and the urechis unicinctus enzymatic hydrolysate is mixed with urechis unicinctus soup juice at a volume ratio of 1:(5-8) evenly to obtain urechis unicinctus seasoning raw liquid. Obviously, the compound urechis unicinctus seasoning materials fully retain the original juice and original taste of the urechis unicinctus, and are rich in nutrition, significant in freshness increasing, and natural and healthy composite seafood seasonings.

The invention belongs to the technical field of biological products, and particularly relates to a preparation method and application of urechis unicinctus autolytic enzyme. The preparation method comprises the steps of taking urechis unicinctus internal organs, performing cleaning with bacteria-free water, performing homogenization, adding PBS, performing leaching, performing loading in low-temperature environment, performing standing overnight, then performing centrifugation, and collecting supernatant; performing gradient precipitating in low-temperature environment with a saturated ammonium sulfate solution to obtain protein precipitate, performing resolvation with PBS buffer liquid, and performing dialysis to obtain an autolytic enzyme crude enzyme solution; and performing column chromatography and separation and purification on the crude enzyme solution to obtain the urechis unicinctus autolytic enzyme. The urechis unicinctus autolytic enzyme prepared by the method has favorablethermal stability, has favorable wall-breaking effects on algal cells of chlorella, and can be applied to extraction of active substances in aquatic animals and processing by-products, and productionand processing of foods including algae, so as to improve degradation effects and reduce production cost.

The invention discloses a breeding method for shortening float periods of urechis unicinctus larvae. The breeding method particularly includes feeding isochrysis 3011 to the urechis unicinctus larvae from the third day to the seventh day after the urechis unicinctus larvae are fertilized, and keeping the temperatures at 15.5-16 DEG C; keeping the water temperatures at 16.5-17 DEG C from the seventh day to the twelfth day; keeping the water temperatures at 17.5-18 DEG C from the thirteenth day to nineteenth day and changing 1/2 volumes of water twice on a daily basis; feeding feed four times on a daily basis from the seventh day to nineteenth day. The isochrysis 3011 is initial feed, and the feeding quantities of the initial feed is 40ml/m<3>d. Formulas and usage quantities of the feed in different procedures from the seventh day to nineteenth day are different from one another. The breeding method has the advantages that the metamorphosis and settlement time of the urechis unicinctus larvae in the float periods can be shortened, breeding periods can be shortened by 15-25 days as compared with schemes in the prior art, and the breeding cost can be reduced to a great extent.

The invention relates to a preparation method of urechis unicinctus viscera glycosaminoglycan. The method comprises the following steps: using urechis unicinctus viscera, rubbing and homogenizing, degreasing and performing pumping filtration to obtain the urechis unicinctus viscera dry powder, then hydrolyzing the viscera dry powder by papain and trypsin, extracting a viscera glycosaminoglycan crude product from a hydrolysate, and performing dialysis and freeze drying to obtain the viscera glycosaminoglycan. The invention also relates to an application of the viscera glycosaminoglycan in preparation of food, health product and medicine for preventing and adjusting blood fat. The extraction method is simple, yield can reach 6-7%, protein content of viscera glycosaminoglycan can be greatly reduced, purity of glycosaminoglycan is increased, and the method provides a novel approach for preventing and adjusting blood fat.

The total RNA of Urechis unicinctus, which is used as template, is cloned to obtain the full-length cDNA sequence of the Urechis unicinctus fibrinolysin gene, wherein the open reading frame (ORF) of the sequence has 792 basic bases and is from the initiation codon ATG to the stop codon TAA. The cDNA original coding sequence of the fibrinolysin gene is translated to obtain the amino acid sequence of the Urechis unicinctus fibrinolysin, wherein the amino acid sequence has 263 amino acids in all. Different recombinant plasmid vectors can be constructed to construct fibrinolysin gene engineering bacteria for different receptors such as Escherichia coli and Saccharomyces and ensure that the cDNA sequence of the Urechis unicinctus fibrinolysin gene is expressed in the gene engineering bacteria and prepare fibrinolysin. The prepared fibrinolysin can be utilized to prepare the anticoagulant medicines or antithrombotic medicines for curing cardiovascular diseases and have very good research and development prospects.

The invention discloses a method for processing urechis unicinctus dumplings and comprehensively utilizing waste of the dumplings. The method comprises the following steps: processing urechis unicinctus: processing the urechis unicinctus fished on the sea in time while keeping the urechis unicinctus fresh, cutting off two ends of the urechis unicinctus, removing impurities, quickly freezing the urechis unicinctus at -40 to -30 DEG C, and conducting storage; processing leek: removing rotten leaves and soil, retaining leek shoots, and removing leek leaves; manufacturing dumplings; treating urechis unicinctus waste: subjecting the urechis unicinctus waste to dehydration and quick-freezing, and preparing a feed for mariculture; and treating leek waste: subjecting the leek waste to dehydration,and preparing a feed for breeding livestock and poultry. The urechis unicinctus is processed by the method of the invention, so that the quality and taste of the urechis unicinctus are ensured, nutrient loss and nutrient deterioration are avoided, the processed dumplings are delicious in taste, fresh, and good in mouth feel, and the limitation that leek urechis unicinctus dumplings prepared by traditional methods can only be prepared when needed is broken.

The invention discloses a Urechis unicinctus feed. The feed is prepared by using the following raw materials, by weight, 25-40 parts of degummed kelp powder, 10-20 parts of gulfweed powder, 8-12 parts of edible seaweed powder, 5-8 parts of soybean protein powder, 8-15 parts of scallop meat powder, 5-10 parts of shrimp shell powder, 5-10 parts of corn germ powder, 1-3 parts of bile acid, 3-6 parts of fish meal, 0.5-1.5 parts of pillared montmorillonite powder, 0.5-0.8 parts of composite vitamin and 0.3-0.5 parts of composite trace element. The Urechis unicinctus feed has the advantages of balanced and comprehensive nutrition and good palatability, allows Urechis unicinctus fed with the feed to have the advantags of good growth, fast growth speed and high weight gain, and benefits the large area popularization culture of the Urechis unicinctus.

The invention relates to urechis unicinctus sperm cryopreservation liquid which comprises urechis unicinctus sperms, preservatives and antifreeze agents. A preparation method of the cryopreservation liquid includes the steps: collecting sperms; identifying sperm activity; diluting the sperms; reducing temperature, and balancing; firstly pre-cooling; secondly pre-cooling; preserving; unfreezing; eluting and the like. According to the cryopreservation liquid, the urechis unicinctus sperms can be effectively preserved for a long time and are preserved in liquid nitrogen, and activation rate of unfrozen sperms can reach 70% or more. According to the preparation method, expensive instruments are omitted, and the preparation method is simple to operate, low in cost and good in effect, provides sperm source guarantee for artificial insemination operations of urechis unicinctus and can be used for other research fields such as crossbreeding, germplasm selection, gynogenesis and species protection of the urechis unicinctus.

The invention discloses a method for stereo ecological aquaculture of urechis unicinctus. The method includes steps of pond repairing, water quality control, fry releasing, bait casting, disease prevention and control, harvesting and the like. By adoption of the method for stereo ecological aquaculture of urechis unicinctus and other organisms, the aquaculture water space and baits can be utilizedcompletely, the aquaculture capacity and unit water yield are increased, economic benefits of mariculture in coastal regions are increased, and important economic significance and social significanceare achieved.

The invention discloses an artificial compound feed capable of promoting growth of blood and blood cells of bodies of urechis unicinctus. The artificial compound feed is characterized by being prepared from the following raw materials in parts by mass of 10-20 parts of soybean flour, 10-20 parts of kelp powder, 20-35 parts of gulfweed powder or sargassum thunbergiio.kuntze powder, 10-20 parts of fish meal, 10-20 parts of clamworm powder, 3-5 parts of blood powder, 5-10 parts of oyster shell powder, 0.5-1 part of complex vitamins, 0.2-0.5 part of composite trace elements, 0.5-1 part of composite traditional Chinese medicine powder and 0.5-1 part of a compound iron preparation. The feed is particulate and the particle size range is 80-150 meshes.