Active polypeptide prepared by high performance liquid chromatography and application thereof

A technology of high-performance liquid chromatography and active peptides, which is applied in the field of marine biological active peptides and its applications, can solve the problems of high fishing intensity and insufficient wild resources

Inactive Publication Date: 2015-12-23
MARINE FISHERIES RES INST OF ZHEJIANG
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to excessive fishing intensity in recent years, wild resources have become insufficient

Method used

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  • Active polypeptide prepared by high performance liquid chromatography and application thereof
  • Active polypeptide prepared by high performance liquid chromatography and application thereof
  • Active polypeptide prepared by high performance liquid chromatography and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0010] Example: a bioactive peptide derived from the viscera of Echinococcus monocylicus, specifically a short peptide with the following sequence: Gln-Pro-Met-Thr-Phe .

[0011] The preparation steps are as follows: (1) The viscera are cut into pieces and then homogenized with a tissue blender. Stir and degrease overnight with acetone, discard the supernatant by centrifugation, dry the precipitate in a blast drying oven at 40°C overnight, and then pulverize it. Add distilled water to the viscera powder of P. monoringensis at a ratio of 1:20 (mass volume ratio, 50g: 1000mL), heat it in a constant temperature water bath at 80°C for 4 hours, centrifuge to get the supernatant, repeat the above steps three times, and finally obtain the supernatant The supernatant is the experimental sample, concentrated. Add distilled water to the sample at a ratio of 1:5 (mass volume ratio, 10g:50mL), then adjust the pH to 6.8, add 2% (mass fraction) papain, heat in a 60°C water bath for 4 hour...

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Abstract

The invention relates to an active polypeptide prepared by high performance liquid chromatography and application thereof, belonging to the technical field of marine organisms. The new short peptide containing 5 amino acid sequences is separated and purified from the protein enzymolysis product of the Urechis unicinctus viscera, the sequence is identified to be Gln-Pro-Met-Thr-Phe, and the ESI-MS detection molecular weight is 552.72 Da. The in-vitro activity indicates that the active polypeptide has favorable angiotensin converting enzyme (ACE) inhibition activity and can be used for preparing anti-hypertension health food.

Description

technical field [0001] The invention relates to a marine biological active polypeptide and its application, in particular to an active polypeptide prepared by high performance liquid chromatography and its application. Background technique [0002] Urechisunicinctus (Urechisunicinctus), commonly known as sea intestines and sea intestines, belongs to the phylum Urechisunicinctus, the order Urechisunicinctus, the order Acanthus, and the family Urechisidae. Large body, about 100-300mm long, 25-27mm wide, 200mm-250mm long, covered with granular protrusions of different sizes, conical snout; 1 pair of abdominal setae, thick; 9-13 around the anus Tail setae brown. Distributed in Russia, Japan, North Korea and the coasts of the Yellow and Bohai Seas in my country, it is a common species of benthic organisms in the intertidal subtidal zone and subtidal shallow water zone on the mud and sandy coast of northern my country. It has a large body, delicious meat, and its body wall muscl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C07K1/20C07K1/16A23L1/305
Inventor 李佩佩张小军严忠雍喻亮张帅陈思
Owner MARINE FISHERIES RES INST OF ZHEJIANG
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