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Preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity

An angiotensin, fermented milk technology, applied to bacteria, dairy products, Lactobacillus and other directions used in food preparation, can solve problems such as impaired renal function, and achieve the effect of improving ACE inhibitory activity and enriching taste

Pending Publication Date: 2021-03-16
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of drugs that inhibit ACE activity can lead to a number of side effects, including cough, rash, and impaired kidney function

Method used

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  • Preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity
  • Preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity
  • Preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:

[0022] Lactococcus lactis ( Lactococcus lactis ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20090, after taking it out from -80°C and thawing, take 200 μL and inoculate it into 5mL sterilized MRS broth medium, and place it in a 37°C constant temperature incubator After 12 hours of static culture in the medium, take 2 mL of the bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 37°C for static culture for 12 hours;

[0023] Yarrowia lipolytica ( Yarrowia lipolytica ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32187, after it was taken out from -80°C and thawed, 200 μL was inoculated into 5mL sterilized YPD mediu...

Embodiment 2

[0036] Embodiment 2: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:

[0037] Lactobacillus helveticus ( Lactobacillus helveticus ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20243, after taking it out from -80 ℃ and thawing, take 150 μL and inoculate it into 5 mL sterilized MRS broth medium, and place it in a 30 ℃ constant temperature incubator After standing for 12 hours, take 1 mL of bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 30°C for 12 hours;

[0038] Kluyveromyces marx ( Kluyveromyces marxianus ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32015, after it was taken out from -80°C and thawed, 150 μL was inoculated into 5mL sterile YPD medium, and placed in a constant t...

Embodiment 3

[0044] Embodiment 3: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:

[0045] Lactobacillus helveticus ( Lactobacillus helveticus ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20243, after taking it out from -80 ℃ and thawing, take 150 μL and inoculate it into 5 mL sterilized MRS broth medium, and place it in a 30 ℃ constant temperature incubator After standing for 12 hours, take 1 mL of bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 30°C for 12 hours;

[0046] Yarrowia lipolytica ( Yarrowia lipolytica ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32187, after it was taken out from -80°C and thawed, 200 μL was inoculated into 5mL sterilized YPD medium, and placed in a constant t...

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Abstract

The invention discloses a preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity. Lactic acid bacteria and saccharomycetes are used for jointly fermenting cow milk to prepare the fermented milk. Lactic acid bacteria and saccharomycetes are used for co-fermentation, the saccharomycetes decompose lactoprotein by secreting protease, peptidase and the like toprovide a basic carbon source for cell growth, and meanwhile, generated small peptides and amino acids provide an important nitrogen source for lactic acid bacteria symbiotic with the small peptidesand amino acids; compared with single strain fermentation, the ACE inhibitory activity of the fermented milk is improved through co-fermentation of lactic acid bacteria and saccharomycetes, a foundation is laid for development of novel functional fermented milk with antihypertensive activity in the future, and the method is simple, easy to operate and suitable for industrial production and marketapplication and popularization.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation and relates to a method for preparing fermented milk, in particular to a method for preparing fermented milk with the activity of inhibiting angiotensin-converting enzyme by co-fermenting milk with lactic acid bacteria and yeast. Background technique [0002] Renin and angiotensin converting enzyme (ACE) are two key enzymes regulating the renin-angiotensin system, and they are important determinants of blood pressure and body fluid homeostasis. Renin cleaves angiotensinogen to produce angiotensin-I, which is then converted into angiotensin-II through the action of ACE. The nonapeptide-bradykinin is inactivated, thereby increasing blood pressure. This gives ACE inhibitors antihypertensive activity. However, the use of drugs that inhibit ACE activity can lead to a number of side effects, including coughing, rashes, and impaired kidney function. Therefore, food-derived ACE inhibito...

Claims

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Application Information

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IPC IPC(8): A23C9/127
CPCA23C9/127A23V2400/137A23V2400/147A23V2400/157A23V2400/165A23V2400/113A23V2400/515A23V2400/169A23V2400/249
Inventor 伊日布斯高杰刘彦敏王康敖日罕安达
Owner KUNMING UNIV OF SCI & TECH
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