Preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity
An angiotensin, fermented milk technology, applied to bacteria, dairy products, Lactobacillus and other directions used in food preparation, can solve problems such as impaired renal function, and achieve the effect of improving ACE inhibitory activity and enriching taste
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0021] Embodiment 1: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:
[0022] Lactococcus lactis ( Lactococcus lactis ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20090, after taking it out from -80°C and thawing, take 200 μL and inoculate it into 5mL sterilized MRS broth medium, and place it in a 37°C constant temperature incubator After 12 hours of static culture in the medium, take 2 mL of the bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 37°C for static culture for 12 hours;
[0023] Yarrowia lipolytica ( Yarrowia lipolytica ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32187, after it was taken out from -80°C and thawed, 200 μL was inoculated into 5mL sterilized YPD mediu...
Embodiment 2
[0036] Embodiment 2: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:
[0037] Lactobacillus helveticus ( Lactobacillus helveticus ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20243, after taking it out from -80 ℃ and thawing, take 150 μL and inoculate it into 5 mL sterilized MRS broth medium, and place it in a 30 ℃ constant temperature incubator After standing for 12 hours, take 1 mL of bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 30°C for 12 hours;
[0038] Kluyveromyces marx ( Kluyveromyces marxianus ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32015, after it was taken out from -80°C and thawed, 150 μL was inoculated into 5mL sterile YPD medium, and placed in a constant t...
Embodiment 3
[0044] Embodiment 3: The preparation method of the fermented milk with the activity of inhibiting angiotensin-converting enzyme is as follows:
[0045] Lactobacillus helveticus ( Lactobacillus helveticus ), purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 20243, after taking it out from -80 ℃ and thawing, take 150 μL and inoculate it into 5 mL sterilized MRS broth medium, and place it in a 30 ℃ constant temperature incubator After standing for 12 hours, take 1 mL of bacterial liquid and inoculate it into 50 mL of sterilized MRS broth medium, and place it in a constant temperature incubator at 30°C for 12 hours;
[0046] Yarrowia lipolytica ( Yarrowia lipolytica ) was purchased from the China Industrial Microorganism Culture Collection Management Center, the strain number is CICC 32187, after it was taken out from -80°C and thawed, 200 μL was inoculated into 5mL sterilized YPD medium, and placed in a constant t...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap