78results about How to "Good ACE inhibitory activity" patented technology

The present invention discloses a method of using biological enzyme decomposition to prepare a hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity, and three novel peptides of high ACE inhibitory activity. For the hydrolysis compound containing peptide of angiotensin transfer enzyme inhibitory activity of the present invention, the oxhide rich in praline is used as the raw material; the product of ACE inhibitory activity IC50 equal to 0.168mg / m can be prepared through the compounding and hydrolysis of biological enzyme. After ultrafiltration, the IC50 is equal to 0.079mg / ml. The means of analysis and detection determines the peptides, ISVPGPM, LGPVGNPGPA and DLSFLPQPPQQ, containing high ACE inhibitory activity. The three peptide sections are novel peptide sections containing high ACE inhibitory activity. Therefore, the three peptides have potential function of lowering blood pressure. The compounding and decomposition of biological enzyme of oxhide is a peptide type hydrolysis product, has inhibitory effects on angiotensin transfer enzyme (ACE), and can be widely used for health food, functional food, auxiliary drugs for lowering blood pressure and other products; the peptide has broad market prospects.

The invention discloses a yak skin blood pressure-decreasing collagen polypeptide and a preparation method thereof. The preparation comprises the following steps: pre-treating raw materials by virtue of physical and chemical methods, preparing gelatin, preparing ACE-inhibiting collagen polypeptide by virtue of composite enzymatic hydrolysis, and separating and purifying polypeptide by utilizing methods such as ultra-filtration, ion-exchange column chromatography, gel filtration chromatography and high performance liquid chromatography; concentrating and drying. According to the technical scheme in the invention, collagen polypeptide is successfully extracted from the yak skin, and the collagen polypeptide has good solubility, emulsifying capacity and foaming characteristic, and has higher ACE-inhibiting activity (blood pressure decreasing effect); the preparation method is simple and easy to operate, and low in cost, so that the application prospect of the yak skin is expanded.

A depressor peptide with higher suppression activity to angiotensin-converting enzyme (ACE) and having 6 amino acids is prepared from the protein of Chinese shrimp through enzymolyzing, separation and purifying. Its short peptide with 12 sequences is chemically synthesized. It can be used to prepare antihypertensive medicine.

The invention provides a loach protein antihypertensive peptide and a preparation method thereof, belonging to the technical field of biology. The antihypertensive short peptide separated and purified from an enzymolysis product of loach protein has high activity in inhibiting angiotensin converting enzyme (ACE). The preparation method provided by the invention comprises the following steps of: carrying out enzymolysis on the loach protein to prepare an enzymolysis liquid; separating and purifying the antihypertensive peptide from the enzymolysis liquid; and sequencing the antihypertensive peptide. The invention combines a modern enzyme engineering technique and a peptide purifying and identifying technique, deep development and utilization on the loach protein are utilized, and a novel-sequence short peptide Leu-Glue-Tyr with high ACE inhibiting activity is found; and the novel loach protein antihypertensive peptide provided by the invention has high ACE inhibiting activity and has an IC50 value of 0.103 mg / mL.

Shark protein antigypertensive peptide, preparation and application thereof, which pertains to marine biology technical field. The novel antigypertensive peptide with two amino acid separated and purified from proteolysis product of shark, has a high inhibiting activity for angiotensin converting enzyme (ACE). Preparation includes steps of carrying out enzymolysis for shark protein to prepare enzymatic hydrolysate, seperating and purifying peptide from the enzymatic hydrolysate, and sequencing of peptide. The invention is applicable for preparing antihypertensive medicament. The invention apply microbial enzyme technology to deep development of shark, obtain peptide having novel sequence, with high inhibiting activity for ACE.

The invention provides an oligopeptide composition, and a delicious seasoning containing the same and a preparation method and application thereof. The oligopeptide composition is prepared from a shellfish or alga through enzymatic hydrolysis under the action of supersonic waves, wherein enzymatic hydrolysis conditions are that the power of the supersonic waves is 250 to 500 W, an enzyme concentration is 2 to 10 mg / g, the mass percent concentration of a substrate is 2 to 10% (in terms of the content of protein), temperature is 45 to 60 DEG C, time is 1 to 10 h and used enzymes comprises flavourzyme and one or more selected from the group consisting of compound protease, neutral protease, papain, alkali protease and pancreatin, and the molecular weight of oligopeptide in the oligopeptide composition is 200 to 4000 Da. The oligopeptide composition has high ACE inhibition activity and unique delicious taste; and the delicious seasoning with a blood pressure-reducing health function and unique flavor can be prepared from the oligopeptide composition.

The invention provides an angiotensin converting enzyme inhibitory peptide derived from bitter almond protein and a preparation method thereof. The inhibitor is pentapeptide of which the relative molecular mass is 688 and the amino acid sequence is Thr-Tyr-Gly-Trp-Tyr. The preparation method comprises the following steps: debitterizing and detoxifying peeled bitter almond raw material, degreasing with petroleum ether, extracting and separating the protein by an alkali fusion acid precipitation method, carrying out enzymolysis with an alkaline proteinase, carrying out centrifugal separation on the enzymolysis solution, taking the supernatant, separating with an ultrafiltration membrane, and separating and purifying by gel filtration chromatography and reversed phase high performance liquid chromatography to obtain the new ACE (angiotensin converting enzyme) inhibitory peptide.

The invention discloses a preparation method of casein hypotensive peptide and belongs to the field of biotechnology. The method comprises the following steps: heat and denature the casein suspension, adjust the pH after cooling, add pepsin, inactivate the enzyme after the reaction is completed, centrifuge, adjust the pH of the supernatant, and then add trypsin and chymotrypsin to continue the reaction. After completion, the enzyme was inactivated, centrifuged, and the supernatant was vacuum concentrated and freeze-dried to obtain the crude casein hypotensive peptide, which was separated by macroporous adsorption resin and Sephadex chromatography to obtain the casein hypotensive peptide with ACE inhibitory activity . The method simulates gastrointestinal digestion and utilizes two-step enzymatic hydrolysis to prepare small molecular peptides, which have high ACE inhibitory activity, no toxic and side effects, and are easy to absorb in vivo, and can be used to prepare antihypertensive drugs and food.

The invention discloses a rapid preparation method of a mussel protein antihypertensive peptide. The antihypertensive peptide is characterized in that an amino acid sequence of the antihypertensive peptide is Val-Ser-Trp-Pro-Cys-Arg-Trp(VSWPCRW); the molecular weight determined by ESI-MS (electrospray ionization mass spectrometry) is 935.07Da. The active peptide Val-Ser-Trp-Pro-Cys-Arg-Trp is obtained by shelling mussels, taking the meat of the mussels, carrying out enzymolysis on the meat, carrying out ultrafiltration on the enzymatic hydrolysate, adsorbing the enzymatic hydrolysate with an agarose magnetic microsphere immobilized ACE (angiotensin converting enzyme) and carrying out purification by RP-HPLC (reversed-phase high-performance liquid chromatography). The active peptide prepared by the invention has obvious ACE inhibitory activity and can be used in hypertension treatment related drugs.

The invention relates to an active polypeptide prepared by high performance liquid chromatography and application thereof, belonging to the technical field of marine organisms. The new short peptide containing 5 amino acid sequences is separated and purified from the protein enzymolysis product of the Urechis unicinctus viscera, the sequence is identified to be Gln-Pro-Met-Thr-Phe, and the ESI-MS detection molecular weight is 552.72 Da. The in-vitro activity indicates that the active polypeptide has favorable angiotensin converting enzyme (ACE) inhibition activity and can be used for preparing anti-hypertension health food.

The invention relates to a manufacturing method for vaccinium bracteatum leaf tea. The method comprises the following steps that 1, stalks of fresh vaccinium bracteatum leaves are removed, and the vaccinium bracteatum leaves are cleaned thoroughly and pulped, distilled water is used for forming a suspension, the suspension is heated, ultrasonic enzymolysis is performed after neutral protease is added to obtain a vaccinium bracteatum leaf proteolysis product; 2, stalks of fresh vaccinium bracteatum leaves are removed, the vaccinium bracteatum leaves are spread to be dried at room temperature and cut into strips, boiling and de-enzyming are performed, then, the vaccinium bracteatum leaves are dried in the shade, baked and smashed to obtain vaccinium bracteatum leaf tea powder; 3, the vaccinium bracteatum leaf proteolysis product and the vaccinium bracteatum leaf tea powder are mixed, packaged and sterilized to obtain a vaccinium bracteatum leaf tea bag product with the anti-hypertensive effect. The technology is simple, operation is convenient, scale production is easy, the obtained product meets the requirement for functional food, and the brewed tea water is light yellow and pan-green, fragrant and heavy in flavor, mellow in taste and sweet in aftertaste and has the anti-hypertensive effect.

the invention discloses a Chinese shrimp protein isallobaric peptide and preparing method and application in the ocean biological technological domain, which is characterized by the following: separating and purifying the isallobaric peptide with 6 amino acids; possessing inhibiting activity of high ACE; proceeding chemical synthesis for shorted-peptide of three 6-amino acids; sieving 12-sequence short peptide with excellent ACE inhibiting activity.

The invention discloses a method for preparing channel catfish skin collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides. The method comprises the following steps: preparing channel catfish skin collagen into a solution with a substrate concentration of 1-3%, adjusting the pH value of the solution to 7.0-8.0; carrying out enzymolysis on the solution for 10-20 min at a temperature of 40-50 DEG C by using alpha-chymotrypsin, wherein the addition amount of the alpha-chymotrypsin is 1000-3000U / g; after the enzymolysis is completed, carrying out enzyme deactivation by using a boiling water bath; adjusting the pH value of reaction mixed liquid to 8.0-9.0; carrying out enzymolysis on the reaction mixed liquid for 20-30min at a temperature of 50-60 DEG C by using Alcalase enzyme, wherein the addition amount of the Alcalase enzyme is 2000-3000U / g; and after the enzymolysis is completed, carrying out enzyme deactivation by using the boiling water bath, and centrifugally collecting liquid supernatant. According to the method, channel catfish skin is used for preparing collagen ACE (angiotensin-1 converting enzyme) inhibitory peptides for the first time, thereby realizing the efficient utilization of channel catfish skin resources; a defect that loca of single enzymatic hydrolysis are insufficient, and loca of mixed enzymatic hydrolysis are interfered with each other is overcome, the ACE inhibitory activity of enzymolysis products is improved, and the IC50 value is 0.33-0.86 mg / mL.

The invention relates to an antihypertensive peptide and a preparation method thereof, and specifically relates to extraction of the antihypertensive peptide from shells. The antihypertensive peptidecomprises the following amino acid sequences: Val-Leu-Asp-Ser-Gly-Asp-Gly-Val-Thr-His; Leu-Asp-Ser-Gly-Asp-Gly-Val-Thr-His; Val-Val-Val-Gly-Asp-Gly-Ala-Val-Gly-Lys; and Phe-Ala-Gly-Asp-Asp-Ala-Pro-Arg-Ala. The ACE inhibitory activity of the antihypertensive peptide prepared in the invention is high, and in the concentration range of lower than 100 micrometers, the antihypertensive peptide is freeof toxic or side effect for vascular endothelial cells and is high in safety; an additional enzymolysis agent is not used in the preparation process, cysteine residues are not contained in the amino acid sequences of the obtained antihypertensive peptide, an additive for protecting the activity of the peptide does not need to be used, the preparation process is simple, the cost is low, and the product quality is high; the raw materials are wide in resources in coastal areas, the cost is low, and the antihypertensive peptide is applicable to industrial production.

The invention belongs to the technical field of biological active peptide. The invention discloses urechis unicinctus biological active peptide, wherein the amino acid sequence is Thr-Arg-Gly-Pro-Pro.The invention also discloses a preparation method of the urechis unicinctus biological active peptide, which is a method for extracting the urechis unicinctus biological active peptide from the bodywall of the urechis unicinctus. The urechis unicinctus biological active peptide has pressure-reducing activity, has high angiotensin converting enzyme (ACE) inhibition activity and can inhibit proliferation of vascular endothelial cells.

The invention provides a method for preparing a hypotensive mulberry leaf tea bag. The method comprises the following steps: removing stalks of fresh mulberry leaves, cleaning the fresh mulberry leaves, pulping and adding protease; performing ultrasonic assisted enzymolysis, and centrifuging after enzymolysis; concentrating supernatant of an enzymolysis product, and lyophilizing to obtain mulberry protein antihypertensive peptide; mixing and blending the mulberry protein antihypertensive peptide and mulberry leaf tea powder; packaging, and sterilizing to obtain the hypotensive mulberry leaf tea bag. The problem of mulberry leaf protein is difficult to hydrolyze due to the existence of pectin can be solved by utilizing ultrasonic wave application in the enzymolysis process, so that the ACE (angiotensin converting enzyme) inhibition activity of the mulberry protein enzymatic hydrolysate can be increased, and the IC 50 (half inhibit concentration) value is 1.12-2.76mg / mL.

The invention discloses angiotensin converting enzyme (ACE) inhibitory peptide which has specific sequence and is screened from porphyra haitanensis enzymolysis products. The sequence of the polypeptide is Thr-Gly-Ala-Pro-Cys-Arg (TGAPCR), and through mass spectrum identification, the molecular weight of the polypeptide is 603.69 Da. The polypeptide has the characteristics of stable structure, notoxicity, high water solubility and the like; and through in-vitro high-performance liquid chromatography (HPLC) identification, the polypeptide has low IC50. Significant effect of reducing the systolic pressure of spontaneously hypertensive rats in vivo is achieved; and the diastolic pressure can be reduced for a long time. The ACE inhibitory peptide with specific sequence can be applied to development of an ACE inhibitor and also can be applied to health-care products and medicine products related to hypertensive therapy.

The invention discloses a preparation method of a sea cucumber egg fermentation product with functions of lowering blood pressure and resisting blood coagulation. The preparation method of the sea cucumber egg fermentation product with the functions of lowering blood pressure and resisting blood coagulation comprises the following steps: washing sea cucumber eggs, and freeze-drying the washed sea cucumber eggs, so that freeze-dried sea cucumber egg powder is prepared for later use; mixing the freeze-dried sea cucumber egg powder with water, adding glucose, and adjusting the initial pH, so that a sea cucumber egg basic culture medium is prepared; sterilizing the basic culture medium; adding a bacillus natto bacterial suspension, and carrying out shaking culturing, so that a sea cucumber egg fermentation broth is prepared; centrifuging the sea cucumber egg fermentation broth, and collecting the obtained supernatant; and then, concentrating the supernatant, and freeze-drying the concentrate, so that the sea cucumber egg fermentation product freeze-dried powder with the functions of lowering blood pressure and resisting blood coagulation is prepared. The sea cucumber eggs are fermented with the bacillus natto according to the preparation method, so that fibrinolytic enzymes with the effect of dissolving thrombus are produced; moreover, polypeptides which are easy to absorb and have the functions of resisting blood coagulation and lowering blood pressure are also produced. The sea cucumber egg fermentation product can be directly eaten as a food, or prepared into health-care functional products after further developments, including concentrating, separating and so on.

The invention discloses a method for preparing antihypertensive peptide by enzymolysis of walnut meal with compound protease, which belong to the technical field of food-borne protein deep processing.The protease preparation is screened and compounded, and enzymolysis process conditions are optimized, so that the walnut protein source antihypertensive peptide with high activity is prepared. The optimal process conditions for enzymolysis of walnut protein are as follows: the ratio of compound enzyme is 1: 2 (alkaline protease: neutral protease), the substrate concentration is 4%, the enzymolysis temperature is 45 DEG C, a pH value is 8.0, the enzyme addition amount is 8000U / g, and the enzymolysis time is 60min. The properties of polypeptide products are compared and analyzed, the relativemolecular mass of the products is mainly concentrated within a range of 0-2 kDa, good normal-temperature storage stability and in-vitro digestion stability are achieved, and a theoretical basis is provided for industrial production and practical application of the products.

The present invention relates to a method for preparing a product containing an antihypertensive peptide by fermenting a starting material containing casein using lactic acid bacteria. The invention also relates to the products obtained and their use in this regard as functional products or as ingredients or additives for edible substances.

The invention belongs to the field of biotechnology and discloses a production method of clam active peptide. The production method of clam active peptide has the following steps: acquiring fresh clammeat and washing with water, and adding water and grinding homogenate by colloid to obtain clam meat slurry; adding water and compound protease in the clam meat slurry to perform enzymolysis and heating to kill enzyme after enzymolysis; and collecting enzymolysis solution through centrifugation, intercepting the enzymatic solution below 2 KDa molecular quantity through microfiltration, ultrafiltration and nanofiltration membrane, drying to obtain the clam active peptide. The production method of clam active peptide takes the flesh claw meat as raw material, adopts compound protease-membrane coupling technique to produce the clam active peptide which has pure color, outstanding taste, and blood pressure lowering functional activity and easy absorption for human body through process technology of enzymolysis, membrane separation and purification, drying and the like, and high-value utilization of the clam meat is achieved.

The invention provides a mytilus crassitesta oligopeptide and use thereof and belongs to the technical field of bioactive peptides. An amino acid sequence of the mytilus crassitesta oligopeptide is Leu-Ala-Ala-Ser-His or Tyr-Glu-Leu-His-Asp. The mytilus crassitesta oligopeptide provided by the invention has relatively high ACE inhibiting activity and is free of cytotoxicity, the content of an endogenous dilatation factor NO in blood vessel endothelial cells (HUVEC cells) can be increased, the content of an endogenous contraction factor ET-1 can be decreased, namely, the pressure release actionis exerted through affecting functions of the blood vessel endothelial cells. The mytilus crassitesta oligopeptide provided by the invention can be applied to preparation of health-care foods and / ormedicines with a blood pressure regulating action.

The invention relates to filefish skin collagen peptide having ACE restraining activity. The amino acid sequence of the collagen peptide is Gly-Val-Gla, and through UPLC-MS detection, the molecular ion peak is [M]+329m / z. The invention further relates to a preparation method of the collagen peptide. The preparation method comprises combined usage of mixing and hydrolysis through double enzymes, ultrafiltration and a gel column chromotography technique, the whole preparation process is simple and easy to control, the enzymolysis efficiency is high, and the obtained collagen peptide is safe andfree from side effects, has higher ACE restraining activity and also has better heat stability; besides, after being digested through digestive tracts of stomach and intestines, the collagen peptide still maintains higher ACE restraining activity; and in addition, processed waste namely fishskin is used as a raw material for preparing the collagen peptide, so that environmental pollution is effectively avoided, and the utilization rate of the resources is increased. The filefish skin collagen peptide has higher practical value and commercial value.

The invention provides a preparation method and application of urechis unicinctus ACE inhibitory peptide, and belongs to the technical field of biological medicine. The preparation method comprises the following steps of degreasing crushed urechis unicinctus, and obtaining a degreased product; drying and crushing the degreased product to obtain urechis unicinctus body wall powder; and mixing the urechis unicinctus body wall powder, neutral protease and water, performing enzymolysis reaction, enzyme deactivation and centrifugation, and collecting supernatant containing the urechis unicinctus ACE inhibitory peptide. The urechis unicinctus ACE inhibitory peptide prepared by the method provided by the invention has good ACE inhibitory activity, and the ACE inhibitory rate of the supernatant containing the urechis unicinctus ACE inhibitory peptide is 52.84 + / -0.31% through measurement.

The invention discloses a preparation method of fermented milk capable of inhibiting angiotensin converting enzyme activity. Lactic acid bacteria and saccharomycetes are used for jointly fermenting cow milk to prepare the fermented milk. Lactic acid bacteria and saccharomycetes are used for co-fermentation, the saccharomycetes decompose lactoprotein by secreting protease, peptidase and the like toprovide a basic carbon source for cell growth, and meanwhile, generated small peptides and amino acids provide an important nitrogen source for lactic acid bacteria symbiotic with the small peptidesand amino acids; compared with single strain fermentation, the ACE inhibitory activity of the fermented milk is improved through co-fermentation of lactic acid bacteria and saccharomycetes, a foundation is laid for development of novel functional fermented milk with antihypertensive activity in the future, and the method is simple, easy to operate and suitable for industrial production and marketapplication and popularization.

Functional food including milk products from which an antihypertensive effect is obtained in a more natural way, more specifically functional food of which ACE inhibitory activity is enhanced by adding EMC preferably with ACE inhibitory activity of 9,000 units or more is provided. ACE inhibitory activity of this functional food is made 5,000 units or more per daily standard intake of the product. For example, in case of processed cheese, ACE inhibitory activity is made 350 units per gram or more assuming daily intake to be approximately 15 g.

The invention relates to the field of active peptide preparation, in particular to a method for preparing antihypertensive active peptides. The method includes carrying out enzymatic hydrolysis on Alaska cod by enzymes; carrying out enzyme deactivation on enzymatic hydrolysate, and then carrying out centrifuging and filtering to obtain the 500-3000 D antihypertensive active peptides which are antihypertensive peptides. The Alaska cod is used as a raw material. The method has the advantages that the antihypertensive active peptides prepared from the Alaska cod by the aid of the method are highin ACE (angiotensin converting enzyme) inhibitory activity and free of toxic and side effect, and can be easily absorbed and utilized by human bodies, and good antihypertensive effects can be realizedby the antihypertensive active peptides; the basis can be provided to comprehensively utilizing cod resources, and the basis can be provided to further developing natural antihypertensive healthcareproducts.

The invention relates to a pig-derived ACE inhibitory activity polypeptide, a pharmaceutical composition or food and application, in particular to a pig-derived polypeptide with angiotensin convertingenzyme (ACE) inhibitory activity and a composition or food containing the active polypeptide or acceptable salt thereof. The amino acid sequence of the polypeptide is Ala-Val-Ala-Thr-Leu-Leu-Lys-Pro.The polypeptide AVATLLKP has ACE inhibitory activity and blood pressure lowering activity, and has good application prospects as a health care product and a medicine lead compound for diseases such as hypertension, heart diseases and cardiovascular diseases.