Acetes chinensis protein antigypertensive peptide and preparation method and application thereof

A technology of antihypertensive peptide and hairy shrimp, applied in the field of marine biology, can solve the problems of low added value, no development and utilization, low added value, etc., and achieve the effect of high ACE inhibition activity

Inactive Publication Date: 2007-02-07
SHANDONG UNIV
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different enzymes are used for enzymatic hydrolysis, and many ACE-inhibiting short peptides are found in the enzymolyzed products. These protein resources include milk protein, soybean protein, egg protein, chicken protein, marine fish protein, algae, etc., until now More than 200 enzymatically active peptides with ACE inhibitory activity have been reported, but the reported ACE inhibitory short peptides are mainly derived from terrestrial protein hydrolysates, and there are fewe

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Acetes chinensis protein antigypertensive peptide and preparation method and application thereof
  • Acetes chinensis protein antigypertensive peptide and preparation method and application thereof
  • Acetes chinensis protein antigypertensive peptide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] (1) Preparation of protease preparation

[0059] Seed culture medium: take 0.3 part of beef extract, 1 part of peptone, 2 parts of agar, 0.5 part of NaCl as medium by weight, 98 parts of water, pH is 7.1, sterilized, streaked and connected to Bacillus subtilis SM98011 (Bacillus.SubtilisSM98011) Bacteria were cultured at 30°C for 20 hours.

[0060] Fermentation medium: 3 parts of bean cake powder, 2 parts of corn flour, 1 part of bran, 40.4 parts of Na2HPO, 0.04 parts of KH2PO, 100 parts of water, and 0.3 part of soybean oil as defoamer. Sterilize at 120°C for 30 minutes, after cooling, put 10 parts of the bacterial suspension connected with seeds into a 150-liter fermenter with a sample volume of 70 liters, and at 30°C, the ventilation rate (V 风 / V 液 ·Time) 1:0.6, stirring at 330 rpm, and culturing for 36 hours. At this point the pH was 7.0.

[0061] (2) Preparation of enzymatic short peptides

[0062] First take by weighing 50 parts (fresh weight) of Chinese hairy...

Embodiment 2

[0070] Embodiment 2: As described in Example 1, the difference is that the amount of raw material hairy shrimp is different, and the hairy shrimp of 45 parts (fresh weight) is beaten. Then add 50 parts (liquid weight) of protease preparation.

[0071] The short peptide of the present invention has high ACE inhibitory activity, and even after entering the digestive tract and undergoing enzymatic hydrolysis, the enzymatic hydrolysis product still has high antihypertensive activity. The present invention is characterized in that it integrates enzyme engineering technology and peptide purification and identification technology, deeply develops and utilizes hairy shrimp, a large amount of marine protein resource that has not been effectively utilized so far, and discovers a new amino acid sequence composition The ACE-inhibiting peptides ensured the functionality in vivo, which laid the foundation for the development of antihypertensive drugs in the future.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

the invention discloses a Chinese shrimp protein isallobaric peptide and preparing method and application in the ocean biological technological domain, which is characterized by the following: separating and purifying the isallobaric peptide with 6 amino acids; possessing inhibiting activity of high ACE; proceeding chemical synthesis for shorted-peptide of three 6-amino acids; sieving 12-sequence short peptide with excellent ACE inhibiting activity.

Description

[0001] This application is a divisional application of No. 200410075836.4 patent application with the same name. (1) Technical field [0002] The invention relates to the separation and purification of an antihypertensive peptide with a new amino acid sequence from an enzymatic hydrolysis product of Chinese hairy shrimp (Accedes chinensis), a preparation method and application thereof, and belongs to the technical field of marine biology. (2) Background technology [0003] Short peptides obtained by partial enzymatic hydrolysis of food proteins have attracted more and more attention from food scientists. These short peptides have various activities, such as: enhancing immunity, increasing nutrient absorption rate, antihypertensive activity. Proteins with these activities have been purified from food protein hydrolysates. These peptides are inactive within the protein sequence and are only active when released from the protein by enzymatic hydrolysis or food processing. [0...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C07K5/06C07K5/08C07K5/10C07K7/06C12P21/02A61K38/04A61P9/12
Inventor 张玉忠何海伦陈秀兰吴昊周百成
Owner SHANDONG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap