This invention discloses a method and application for the separation and purification of polysaccharides from *Tea laurentii*, belonging to the field of *Tea laurentii* technology. The method includes pulverizing and sieving *Tea laurentii*, extracting with
ethanol, centrifuging to collect the precipitate; adjusting the pH of the precipitate with water, adding a compound
enzyme for
enzymatic hydrolysis, centrifuging to collect the supernatant, vacuum concentrating, and precipitating with
alcohol by
centrifugation to obtain a crude
polysaccharide extract. The extract is then further purified by adding water to adjust the pH, adding
papain for
enzymatic hydrolysis, heating and holding at a constant temperature, adding a compound
solvent, centrifuging to collect the upper aqueous phase, treating with
petroleum ether, adsorbing with a compound adsorbent, and
dialysis to obtain refined crude polysaccharides. These are then further purified by
chromatography,
gradient elution, concentration
dialysis, and freeze-
drying to finally obtain *Tea laurentii* polysaccharides. This invention utilizes a multi-step synergistic purification process involving compound
enzymatic hydrolysis, compound
solvent removal, compound adsorbent adsorption, and
gradient elution to effectively remove proteins, fat-soluble pigments,
small molecule impurities, and salts from *Tea laurentii* polysaccharides, significantly improving the purity and uniformity of the product.