5 results about "Alp activity" patented technology

This invention discloses a near-infrared fluorescent probe for detecting bone regeneration vitality and its preparation method, belonging to the field of biochemistry. Using rhodamine as the fluorescent core, the invention utilizes quinoline ring expansion to increase the conjugated area and extend emission. The brightness and stability of the probe are controlled by adjusting the steric hindrance of the aromatic ring at position 9, constructing a series of stable, high-brightness probe cores with low pKa. By introducing a phosphate ester group as a responder while simultaneously quenching the fluorescence of the core, a near-infrared fluorescent probe, SiRd-MOP, for detecting bone regeneration vitality is obtained. The preparation method of this invention has high yield and mild reaction conditions. The prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), and has successfully achieved ALP activity monitoring in osteogenic differentiation models and throughout the entire process of fracture regeneration and repair in mice. It can be used for real-time assessment of bone regeneration vitality and has very high biological application prospects.

The application belongs to the technical field of nano-enzyme sensing, and particularly relates to a three-mode phytic acid detection method based on a double-enzyme cascade reaction and application thereof. The detection method uses CAU-10-NH2 metal organic framework as a light-responsive nano-enzyme, combines the light catalytic oxidase activity of ALP and CAU-10-NH2, acquires signals through three modes of fluorescence spectrum, ultraviolet-visible absorption spectrum and photothermal imaging, and quantitatively analyzes the phytic acid content according to the signal change. Phytic acid inhibits the ALP activity, controls the generation amount of ascorbic acid, further influences the oxTMB content generated by CAU-10-NH2 catalyzing TMB oxidation, and realizes the synchronous change of fluorescence, colorimetric and photothermal signals. The application first discovers that CAU-10-NH2 has light catalytic oxidase activity, and only the -NH2 substituted derivative in the CAU-10 series (including CAU-10-H, -OH, -Br, -NO2, etc.) exhibits the activity, which expands the application of aluminum-based MOFs in the field of nano-enzyme sensing.

The invention discloses a near-infrared fluorescent probe for detecting bone regeneration activity and a preparation method of the near-infrared fluorescent probe, and relates to the technical field of biochemistry. According to the invention, rhodamine is taken as a fluorescent parent nucleus, quinoline ring expansion is utilized to enlarge the conjugate area and prolong emission, and the brightness and stability of the probe are regulated by regulating the steric hindrance of a ninth aromatic ring, so that a series of stable and high-brightness probe parent nucleuses with smaller pKa are constructed. The near-infrared fluorescent probe SiRd-MOP for detecting the bone regeneration activity is obtained by introducing a phosphate ester group as a response group and quenching parent nucleus fluorescence at the same time. The preparation method is high in yield and mild in reaction condition, the prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), ALP activity monitoring in the whole process of fracture regeneration and repair of a cell osteogenic differentiation model and a mouse is successfully achieved, the probe can be used for evaluating bone regeneration activity in real time, and the probe has very high biological application prospects.

The invention belongs to the technical field of biosensors, and relates to a biosensor for detecting the total amount of pesticide residues as well as a detection method and application of the biosensor. The biosensor comprises alkaline phosphatase, a buffer solution, ascorbic acid-2-phosphate, a double-chain probe, an activation chain, a fluorescence report probe and Cu < 2 + >. The double-stranded probe is formed by hybridizing DNA1 and DNA2, one side of the blunt end of the double-stranded probe is respectively modified with an azide group and an alkynyl group, and one side of the overhanging end of the double-stranded probe is provided with a toehold region and a section of MNAzyme fragment; the activation chain comprises another MNAzyme segment, and the activation chain can be hybridized with the DNA1 to form the MNAzyme. Alkaline phosphatase is used as a broad-spectrum indicator, a collective normalization biosensing strategy for synchronous toxicity detection of multiple pesticide residues is developed based on ALP activity total inhibition rate detection, high sensitivity, operability and multi-component response capability are achieved, and early, rapid and comprehensive monitoring of the pesticide residues is facilitated.

This invention discloses an antibacterial and osteogenic formulation of zinc-doped mesoporous silica nanoparticles and its preparation method. A mesoporous silica precursor is prepared using a template method, and a zinc precursor is added in stages during the hydrolysis and condensation process of the silica source, ensuring uniform dispersion of the zinc component in the framework, pore walls, and surface layer. After heat treatment to remove the template, zinc-doped mesoporous silica nanoparticles (Zn-MSN) are obtained. Further surface modification with amylation, loading with antibacterial agents and / or osteogenic active components, and construction of an end-capping layer yield a multifunctional formulation. The formulation has an average particle size of 80–200 nm and a specific surface area of ​​150–350 m² / g. 2 / g, the zinc component has no independent crystalline phase, which can realize the time-sequential release of zinc ions and active components; in vitro experiments show that the formulation has an antibacterial rate of >99% against Staphylococcus aureus and other bacteria, and can significantly enhance ALP activity, mineralized nodule formation and RUNX2 / OCN expression, and has both antibacterial and osteopromoting synergistic effects, making it suitable for the repair of infected bone defects.