11 results about "Alp activity" patented technology

The invention discloses a method for inducing osteoblasts by using mesenchymal stem cells, and belongs to the technical field of biology. The method for inducing the osteoblasts comprises the step of adding brucella outer membrane protein into a conventional osteoblast induction culture medium to promote the dental pulp stem cells to be differentiated into the osteoblasts. According to osteoblast differentiation metaphase differentiation marker detection, the cell ALP activity of a group added with 800ng / mL of brucella outer membrane protein is 2.36 times that of a conventional osteoblast induction culture medium group; according to osteoblast differentiation end-stage differentiation marker detection, the calcium deposition amount of brucella outer membrane proteome cells added with 800ng / mL is 481.54 mu g / mg protein, and is 2.55 times of that of a conventional osteoblast induction culture medium group. By adding the brucella outer membrane protein into a conventional osteoblast induction culture medium, the osteogenic differentiation efficiency of the dental pulp stem cells can be effectively improved.

The invention discloses copper selenide / tricalcium silicate bone cement. The bone cement comprises a liquid phase component and a solid phase component, the liquid phase component is water, and the solid phase component is tricalcium silicate powder and copper selenide nanoparticles. Compared with the existing tricalcium silicate bone cement, the tricalcium silicate bone cement has the advantages that by introducing a trace amount of copper selenide nanoparticles, not only can the hydration and coagulation time of tricalcium silicate be shortened, but also the porosity and degradation rate of the tricalcium silicate after coagulation and solidification can be reduced, the compressive strength can be improved, the stimulation effect on ALP activity of MC3T3-E1 cells can be enhanced, the survival rate of the MC3T3-E1 cells can be improved, and the application prospect is broad. The immunogenic apoptosis of K7M2-wt cells is promoted, and the tumor growth rate is reduced. Besides, the introduction of the copper selenide nanoparticles can also endow the tricalcium silicate bone cement with the performance of temperature rise under near-infrared light, so that in clinical application, the tricalcium silicate bone cement can be further combined with a near-infrared technology to realize ablation of bone tumors, and a new strategy is provided for treatment of the bone tumors.

The invention relates to the technical field of tissue engineering and regeneration, in particular to a stem cell membrane-stent dynamic co-culture device and a gradient regulation culture process. The stem cell membrane-stent dynamic co-culture device comprises a) a multi-modal mechanical loading module (1), a compression strain unit (2) and a multi-modal mechanical loading module (3), the frequency is adjustable from 0.1 Hz to 2 Hz, the strain amplitude is 5%-15% (the precision is + / -0.5%), and the periodic compression stress of bone tissue can be simulated; (2) a fluid shear force unit: generating 0.1-5Pa laminar shear force (Re < 2000) through a conical flow channel (with a taper angle of 15 degrees) and a peristaltic pump, and simulating a shear microenvironment of a blood vessel or periodontal ligament area; the method has the beneficial effects that multi-modal mechanical synergy is achieved, and the cell differentiation efficiency is remarkably improved through coupling loading of compression and shearing force. Experiments show that the ALP activity reaches 38.2 + / -3.1 U / mg (only 12.5 + / -2.3 U / mg in traditional single-mode stimulation), and the proportion of a CD31 + vascularized region is increased to 41.7 + / -3.8% (plt; 0.01).

The invention discloses a preparation method of a BMP-2 sustained-release material and application of the BMP-2 sustained-release material in bone defect repair, and belongs to the technical field of drug sustained release. Boric acid, urea and PMMA microspheres are used for preparing a boron nitride porous material, and after the boron nitride porous material is modified through O / Ar mixed gas treatment, the BMP-2 sustained-release material is prepared. The modified boron nitride porous material is soaked in an SBF solution and a BMP-2 solution, the prepared BMP-2 sustained-release material serves as a sustained-release material, the degradation speed of the BMP-2 sustained-release material is low, in-vivo slow release of BMP-2 can be achieved, and cell ALP activity is high.

This invention discloses a near-infrared fluorescent probe for detecting bone regeneration vitality and its preparation method, belonging to the field of biochemistry. Using rhodamine as the fluorescent core, the invention utilizes quinoline ring expansion to increase the conjugated area and extend emission. The brightness and stability of the probe are controlled by adjusting the steric hindrance of the aromatic ring at position 9, constructing a series of stable, high-brightness probe cores with low pKa. By introducing a phosphate ester group as a responder while simultaneously quenching the fluorescence of the core, a near-infrared fluorescent probe, SiRd-MOP, for detecting bone regeneration vitality is obtained. The preparation method of this invention has high yield and mild reaction conditions. The prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), and has successfully achieved ALP activity monitoring in osteogenic differentiation models and throughout the entire process of fracture regeneration and repair in mice. It can be used for real-time assessment of bone regeneration vitality and has very high biological application prospects.

The application belongs to the technical field of nano-enzyme sensing, and particularly relates to a three-mode phytic acid detection method based on a double-enzyme cascade reaction and application thereof. The detection method uses CAU-10-NH2 metal organic framework as a light-responsive nano-enzyme, combines the light catalytic oxidase activity of ALP and CAU-10-NH2, acquires signals through three modes of fluorescence spectrum, ultraviolet-visible absorption spectrum and photothermal imaging, and quantitatively analyzes the phytic acid content according to the signal change. Phytic acid inhibits the ALP activity, controls the generation amount of ascorbic acid, further influences the oxTMB content generated by CAU-10-NH2 catalyzing TMB oxidation, and realizes the synchronous change of fluorescence, colorimetric and photothermal signals. The application first discovers that CAU-10-NH2 has light catalytic oxidase activity, and only the -NH2 substituted derivative in the CAU-10 series (including CAU-10-H, -OH, -Br, -NO2, etc.) exhibits the activity, which expands the application of aluminum-based MOFs in the field of nano-enzyme sensing.

The invention discloses a near-infrared fluorescent probe for detecting bone regeneration activity and a preparation method of the near-infrared fluorescent probe, and relates to the technical field of biochemistry. According to the invention, rhodamine is taken as a fluorescent parent nucleus, quinoline ring expansion is utilized to enlarge the conjugate area and prolong emission, and the brightness and stability of the probe are regulated by regulating the steric hindrance of a ninth aromatic ring, so that a series of stable and high-brightness probe parent nucleuses with smaller pKa are constructed. The near-infrared fluorescent probe SiRd-MOP for detecting the bone regeneration activity is obtained by introducing a phosphate ester group as a response group and quenching parent nucleus fluorescence at the same time. The preparation method is high in yield and mild in reaction condition, the prepared probe can specifically and sensitively respond to alkaline phosphatase (ALP), ALP activity monitoring in the whole process of fracture regeneration and repair of a cell osteogenic differentiation model and a mouse is successfully achieved, the probe can be used for evaluating bone regeneration activity in real time, and the probe has very high biological application prospects.

The invention belongs to the technical field of biosensors, and relates to a biosensor for detecting the total amount of pesticide residues as well as a detection method and application of the biosensor. The biosensor comprises alkaline phosphatase, a buffer solution, ascorbic acid-2-phosphate, a double-chain probe, an activation chain, a fluorescence report probe and Cu < 2 + >. The double-stranded probe is formed by hybridizing DNA1 and DNA2, one side of the blunt end of the double-stranded probe is respectively modified with an azide group and an alkynyl group, and one side of the overhanging end of the double-stranded probe is provided with a toehold region and a section of MNAzyme fragment; the activation chain comprises another MNAzyme segment, and the activation chain can be hybridized with the DNA1 to form the MNAzyme. Alkaline phosphatase is used as a broad-spectrum indicator, a collective normalization biosensing strategy for synchronous toxicity detection of multiple pesticide residues is developed based on ALP activity total inhibition rate detection, high sensitivity, operability and multi-component response capability are achieved, and early, rapid and comprehensive monitoring of the pesticide residues is facilitated.

The present invention relates to the technical field of food safety detection, and in particular to a method for detecting glyphosate by an in-situ fluorescence reaction triggered by a biological enzyme. The present invention utilizes alkaline phosphatase (ALP) to catalyze the substrate L-ascorbic acid-2-phosphate trisodium salt to form L-ascorbic acid, which reacts with 3-aminopropyltrimethoxysilane at room temperature to generate fluorescent product silicon nanoparticles (SiNPs). When glyphosate is present, glyphosate inhibits ALP activity, weakens its catalytic ability, and then causes L-ascorbic acid and SiNPs to produce less, and the fluorescence intensity weakens. As the concentration of glyphosate increases, the fluorescence intensity of the system continuously weakens, thereby achieving quantitative detection of glyphosate. The present invention does not need to prepare nanomaterials, simplifies the detection method, reduces detection costs, and simultaneously the method has good selectivity and high sensitivity in the detection of glyphosate, and has a broader application prospect in glyphosate residue detection.

This invention discloses an antibacterial and osteogenic formulation of zinc-doped mesoporous silica nanoparticles and its preparation method. A mesoporous silica precursor is prepared using a template method, and a zinc precursor is added in stages during the hydrolysis and condensation process of the silica source, ensuring uniform dispersion of the zinc component in the framework, pore walls, and surface layer. After heat treatment to remove the template, zinc-doped mesoporous silica nanoparticles (Zn-MSN) are obtained. Further surface modification with amylation, loading with antibacterial agents and / or osteogenic active components, and construction of an end-capping layer yield a multifunctional formulation. The formulation has an average particle size of 80–200 nm and a specific surface area of ​​150–350 m² / g. 2 / g, the zinc component has no independent crystalline phase, which can realize the time-sequential release of zinc ions and active components; in vitro experiments show that the formulation has an antibacterial rate of >99% against Staphylococcus aureus and other bacteria, and can significantly enhance ALP activity, mineralized nodule formation and RUNX2 / OCN expression, and has both antibacterial and osteopromoting synergistic effects, making it suitable for the repair of infected bone defects.

The present invention discloses a preparation method of a BMP-2 sustained-release material and an application thereof in bone defect repair, belonging to the technical field of drug sustained-release. The present invention uses boric acid, urea and PMMA microspheres to prepare a boron nitride porous material. After the boron nitride porous material is modified by treating it with an O₂ / Ar mixed gas, the modified boron nitride porous material is soaked in an SBF solution and a BMP-2 solution. The prepared BMP-2 sustained-release material serves as a sustained-release material, has a slow self-degradation rate, can achieve the slow release of BMP-2 in the body, and has high cellular ALP activity.