The invention belongs to the technical field of
analytical chemistry, and relates to an
alkaline phosphatase activity colorimetric detection method based on CeVO4. The
alkaline phosphatase activity colorimetric detection method comprises the following steps: respectively adding
alkaline phosphatase with different activities and 0.05 mL of 1 mM
sodium hexametaphosphate (NaPO3) 6 into a 5 mL
centrifuge tube; sequentially adding 0.1 mL of 1mg / mL CeVO4 suspension, 0.1 mL of 5mM TMB and an acetate
buffer solution into the
mixed solution, wherein the final total volume of the solution is 3mL; afterpassing through the membrane, recording the
absorbance at the
wavelength of 652 nm by using an
ultraviolet-visible absorption spectrophotometer, and drawing an
ALP activity-
absorbance standard workingcurve; performing calibration; and measuring the
absorbance of the sample to be measured, and comparing to obtain the
ALP activity. The method is mild in detection process condition, low in detectioncost and simple to operate; the alkaline
phosphatase activity is detected through a TMB + CeVO4
system, the
detection limit is as low as 0.68 U / L, and the detectable range is as wide as 1-210 U / L; the alkaline
phosphatase activity is detected by using a TMB + CeVO4
system, the response to alkaline
phosphatase is sensitive, the measurement result of an actual sample is accurate and reliable, and the detection of the alkaline phosphatase activity in human serum is realized.