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A kind of vegf-165 activator and its application for promoting stem cell differentiation

A technology of stem cells and stromal cells, applied in the field of osteogenic differentiation of stem cells, which can solve the problems of infection, immune rejection, etc.

Active Publication Date: 2021-07-06
ASIA REGENERATIVE MEDICINE LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection.

Method used

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  • A kind of vegf-165 activator and its application for promoting stem cell differentiation
  • A kind of vegf-165 activator and its application for promoting stem cell differentiation
  • A kind of vegf-165 activator and its application for promoting stem cell differentiation

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Experimental program
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Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

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Abstract

The invention relates to a VEGF-165 activator and its use for promoting stem cell differentiation. Alkaline phosphatase (ALP) is a marker of early osteogenesis, which is mainly distributed in the cell membrane and promotes cell calcification. The quantitative detection of ALP can reflect the differentiation level of osteoblasts. Cell differentiation is more obvious. High expression of ALP activity is an early marker of osteoblast differentiation and maturation. When ALP activity is enhanced, bone formation is enhanced and bone matrix mineralization is promoted. Therefore, ALP activity is a good indicator to reflect the degree of differentiation and functional status of osteoblasts. Test compounds 1-3 promote the osteogenic differentiation of hBMSCs by activating the expression of BMP-2 protein in hBMSCs, and test compounds 4 and 5 promote the osteogenic differentiation of hBMSCs by activating the expression of VEGF-165 protein in hBMSCs.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/165C12N2506/1353
Inventor 卢珊珊赵元英
Owner ASIA REGENERATIVE MEDICINE LTD
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