36 results about "Pre osteoblasts" patented technology

The invention discloses a bone-promoting rubidium-titanium-tantalum composite material and its preparation method and application. The method performs sand blasting, acid treatment and alkali heat treatment on the titanium-tantalum composite material synthesized by plasma activation and sintering to modify the surface of the titanium-tantalum composite material. properties, and the surface of the surface-modified titanium-tantalum composite material is doped with rubidium salt through ion exchange, and finally the bone-promoting rubidium-titanium-tantalum composite material is prepared by high-temperature calcination. The material of the invention has good biocompatibility, does not have cytotoxicity, promotes cell proliferation, and has a good effect of inducing osteogenic differentiation of pre-osteoblasts. The material can effectively solve the problems of poor compatibility with the human body in clinical application of bone repair materials, and has broad application prospects in clinical bone repair.

The invention relates to a bone morphogenetic protein 2 activator and its use for promoting osteogenic differentiation of mesenchymal stem cells. Alkaline phosphatase (ALP) is a marker of early osteogenesis, which is mainly distributed in the cell membrane and promotes cell calcification. The quantitative detection of ALP can reflect the differentiation level of osteoblasts. Cell differentiation is more obvious. High expression of ALP activity is an early marker of osteoblast differentiation and maturation. When ALP activity is enhanced, bone formation is enhanced and bone matrix mineralization is promoted. Therefore, ALP activity is a good indicator to reflect the degree of differentiation and functional status of osteoblasts. Test compounds 1-3 promote the osteogenic differentiation of hBMSCs by activating the expression of BMP-2 protein in hBMSCs, and test compounds 4 and 5 promote the osteogenic differentiation of hBMSCs by activating the expression of VEGF-165 protein in hBMSCs.

The invention belongs to the technical field of medicines, and particularly relates to application of compound tarasaponin IV capable of promoting primary osteoblast multiplication and mineralizationfunctions. The invention provides new application of the tarasaponin IV, i.e., application of the tarasaponin IV in preparation of medicine for preventing or treating bone diseases. The result shows that the tarasaponin IV can promote the preosteoblast growth and increase the demand quantity on ALP, so that the ALP utilization rate is improved; the formation of osteoblast mineralized nodules is promoted; the bone matrix mineralization effect is improved, so that the tarasaponin IV can be used as an active ingredient for preparing the medicine for preventing or treating the bone diseases. The medicine can be any one form of tablets, pills, capsules or injections. The invention also provides a preparation method of the medicine.