36 results about "Pre osteoblasts" patented technology

The present invention relates to a pharmaceutical composition for preventing or treating a bone-related disease; and the pharmaceutical composition is very effective in preventing or treating bone-related diseases in that it not only promotes differentiation of preosteoblast cells into osteoblasts to induce production of mineralized nodules, but also, at the same time, inhibits differentiation of bone marrow cells into osteoclasts and the function of osteoclasts so that bone homeostasis is effectively regulated. In addition, the present invention relates to a pharmaceutical composition for preventing or treating a menopausal disease, in particular, a menopausal bone disease; and the pharmaceutical composition is very effective in preventing or treating a menopausal bone-related disease in that it not only increases the volume of trabecular bone and promotes differentiation of preosteoblast cells into osteoblasts to induce production of mineralized nodules, but also, at the same time, inhibits differentiation of bone marrow cells into osteoclasts and the function of osteoclasts so that bone homeostasis is effectively regulated.

The invention discloses an shRNA sequence for inhibiting mouse MACF1 gene expression and application thereof, which is used to solve the technical problem of low efficiency of the existing shRNA sequence for inhibiting mouse MACF1 gene expression. The technical solution is to clone the shRNA sequence that inhibits the expression of the mouse MACF1 gene into the pGLV3 lentiviral vector to obtain a recombinant lentiviral vector containing the shRNA sequence; the obtained recombinant vector is used to transfect mouse cells to achieve inhibition Purpose of MACF1 gene expression in cells. The shRNA sequence mentioned above can obviously inhibit the expression of MACF1 gene at mRNA and protein levels in mouse preosteoblasts. The proliferation, migration and differentiation functions of preosteoblasts were inhibited by inhibiting the expression of MACF1 in mouse preosteoblasts. The inhibition rate of the present invention at the protein level reaches over 90%, and the inhibition rate at the gene level reaches 81%.

The invention discloses a bone-promoting rubidium-titanium-tantalum composite material and its preparation method and application. The method performs sand blasting, acid treatment and alkali heat treatment on the titanium-tantalum composite material synthesized by plasma activation and sintering to modify the surface of the titanium-tantalum composite material. properties, and the surface of the surface-modified titanium-tantalum composite material is doped with rubidium salt through ion exchange, and finally the bone-promoting rubidium-titanium-tantalum composite material is prepared by high-temperature calcination. The material of the invention has good biocompatibility, does not have cytotoxicity, promotes cell proliferation, and has a good effect of inducing osteogenic differentiation of pre-osteoblasts. The material can effectively solve the problems of poor compatibility with the human body in clinical application of bone repair materials, and has broad application prospects in clinical bone repair.

The invention relates to a bone morphogenetic protein 2 activator and its use for promoting osteogenic differentiation of mesenchymal stem cells. Alkaline phosphatase (ALP) is a marker of early osteogenesis, which is mainly distributed in the cell membrane and promotes cell calcification. The quantitative detection of ALP can reflect the differentiation level of osteoblasts. Cell differentiation is more obvious. High expression of ALP activity is an early marker of osteoblast differentiation and maturation. When ALP activity is enhanced, bone formation is enhanced and bone matrix mineralization is promoted. Therefore, ALP activity is a good indicator to reflect the degree of differentiation and functional status of osteoblasts. Test compounds 1-3 promote the osteogenic differentiation of hBMSCs by activating the expression of BMP-2 protein in hBMSCs, and test compounds 4 and 5 promote the osteogenic differentiation of hBMSCs by activating the expression of VEGF-165 protein in hBMSCs.

The invention discloses an antibacterial and bone-containing rubidium-titanium-tantalum composite material and a preparation method thereof. In the method, the titanium-tantalum composite material synthesized by cold isostatic pressing and vacuum sintering is subjected to sandblasting, acid treatment and alkali heat treatment to make titanium-tantalum composite The surface of the material is modified, and the surface of the surface-modified titanium-tantalum composite material is doped with rubidium salt through ion exchange, and finally the antibacterial bone-promoting rubidium-titanium-tantalum composite material is prepared by high-temperature calcination. The material of the invention has good antibacterial properties and biocompatibility, does not have cytotoxicity, promotes cell proliferation, and has a good effect of inducing osteogenic differentiation of pre-osteoblasts. The material can effectively solve the problems of easy infection of bacteria in clinical application of bone repair materials and poor compatibility between implant materials and human body, and has broad application prospects in clinical bone repair.

The invention belongs to the technical field of medicines, and particularly relates to application of compound tarasaponin IV capable of promoting primary osteoblast multiplication and mineralizationfunctions. The invention provides new application of the tarasaponin IV, i.e., application of the tarasaponin IV in preparation of medicine for preventing or treating bone diseases. The result shows that the tarasaponin IV can promote the preosteoblast growth and increase the demand quantity on ALP, so that the ALP utilization rate is improved; the formation of osteoblast mineralized nodules is promoted; the bone matrix mineralization effect is improved, so that the tarasaponin IV can be used as an active ingredient for preparing the medicine for preventing or treating the bone diseases. The medicine can be any one form of tablets, pills, capsules or injections. The invention also provides a preparation method of the medicine.