Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Bone morphogenetic protein 2 activator for inducing directed differentiation of stem cells

A technology of morphogenetic protein and activator, applied in the field of stem cells, which can solve problems such as infection and immune rejection

Active Publication Date: 2018-11-30
广东创晟控股集团有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bone morphogenetic protein 2 activator for inducing directed differentiation of stem cells
  • Bone morphogenetic protein 2 activator for inducing directed differentiation of stem cells
  • Bone morphogenetic protein 2 activator for inducing directed differentiation of stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a bone morphogenetic protein 2 activator for promoting directed differentiation of stem cells. Alkaline phosphatase (ALP) is an early osteogenesis marker, and is mainly distributed in cell membranes to promote cell calcification. The quantitative detection of the ALP can reflect the differentiation level of osteoblasts. If the activity of the ALP is higher, the higher activity of the ALP indicates that pre-osteoblasts have more obvious differentiation tendency to mature osteoblasts. The high expression of the activity of the ALP is an early marker of osteoblastdifferentiation and maturation. When the activity of the ALP is enhanced, bone formation is enhanced, and bone matrix mineralization is promoted. Therefore, the activity of the ALP is a good indicator for reflecting the degree differentiation and the functional status of the osteoblasts. The osteogenic differentiation of hBMSCs can be promoted by activating BMP-2 protein expression in the hBMSCsby to-be-tested compounds 1 to 3. The osteogenic differentiation of the hBMSCs can be promoted by activating VEGF-165 protein expression in the hBMSCs by to-be-tested compounds 4 and 5.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/155C12N2506/1353
Inventor 卢珊珊赵元英
Owner 广东创晟控股集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com