A kind of activator of vascular endothelial growth factor 165 and its application in stem cells

A technology of stem cells and stromal cells, applied in the field of osteogenic differentiation of stem cells, which can solve the problems of infection, immune rejection, etc.

Active Publication Date: 2021-10-01
广东创晟控股集团有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of activator of vascular endothelial growth factor 165 and its application in stem cells
  • A kind of activator of vascular endothelial growth factor 165 and its application in stem cells
  • A kind of activator of vascular endothelial growth factor 165 and its application in stem cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a vascular endothelial growth factor 165 activator and its application in stem cells. Alkaline phosphatase (ALP) is a marker of early osteogenesis, which is mainly distributed in the cell membrane and promotes cell calcification. The quantitative detection of ALP can reflect the differentiation level of osteoblasts. Cell differentiation is more obvious. High expression of ALP activity is an early marker of osteoblast differentiation and maturation. When ALP activity is enhanced, bone formation is enhanced and bone matrix mineralization is promoted. Therefore, ALP activity is a good indicator to reflect the degree of differentiation and functional status of osteoblasts. Test compounds 1-3 promote the osteogenic differentiation of hBMSCs by activating the expression of BMP-2 protein in hBMSCs, and test compounds 4 and 5 promote the osteogenic differentiation of hBMSCs by activating the expression of VEGF-165 protein in hBMSCs.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/999C12N2506/1353
Inventor 卢珊珊赵元英
Owner 广东创晟控股集团有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products