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Method for inducing bone mesenchymal stem cells to osteoblast differentiation

A technology for osteoblast differentiation and bone marrow mesenchymal, which is applied to bone/connective tissue cells, biochemical equipment and methods, animal cells, etc. Simple and convenient, risk avoidance and immunogenicity, low cost effect

Inactive Publication Date: 2017-06-27
周婧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods are relatively complicated for the preparation of materials, and the proportion of osteoblast differentiation is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Clean the surface of the strontium titanate {100} crystal surface single wafer with deionized water in ultrasonic wave; irradiate the cleaned strontium titanate {100} crystal surface single wafer under ultraviolet light (wavelength 365nm, power 15W), The irradiation time was 30min; the ratio of terminal hydroxyl group / bridging hydroxyl group formed in situ on the surface of strontium titanate {100} crystal plane was 0.3; 4 / cm 2 The density of strontium titanate was uniformly planted on a single chip of strontium titanate {100} crystal surface, cultured with conventional DMEM basal medium, and the medium was changed every two days; after 14 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The proportion of osteoblasts was 60%.

Embodiment 2

[0011] Clean the surface of the strontium titanate {100} crystal surface single wafer with deionized water in ultrasonic wave; irradiate the cleaned strontium titanate {100} crystal surface single wafer under ultraviolet light (wavelength 365nm, power 15W), The irradiation time was 60min; the ratio of terminal hydroxyl group / bridging hydroxyl group formed in situ on the surface of strontium titanate {100} crystal facet single crystal was 0.4; 4 / cm 2 The density of strontium titanate was uniformly planted on a single chip of strontium titanate {100} crystal surface, cultured with conventional DMEM basal medium, and the medium was changed every two days; after 14 days of culture, mature and differentiated cells were obtained by flow cytometry identification. The proportion of osteoblasts was 75%.

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Abstract

The invention discloses a method for inducing bone mesenchymal stem cells to osteoblast differentiation. A crystal face single crystal surface of strontium titanate {100} is irradiated with ultraviolet light firstly to produce a surface with specific hydroxyl properties, then bone mesenchymal stem cells are cultured on the surface, preosteoblasts can be obtained after the culturing for 7 days by using the conventional DMEM basal medium, and mature differentiation osteoblasts can be obtained after the culturing for 14 days. The method for inducing directional differentiation of the stem cells by utilizing hydroxyl properties of the material surface, provided by the invention, is simple and convenient to operate and very low in cost, can obviously improve the differentiation ratio of the mesenchymal stem cells into the osteoblasts, and further can avoid application risks and immunogenicity of animal serums and biochemical reagents, thereby being applicable to the fields of bone tissue repair, reconstruction and the like.

Description

technical field [0001] The invention relates to a method for inducing bone marrow mesenchymal stem cells to differentiate into osteoblasts. Background technique [0002] Stem cell directional differentiation regulation is one of the key topics in stem cell research. In addition to biochemical methods such as gene transcription factors, cell growth factors, and chemical inducers, it has been found that the surface properties of materials play a decisive role in mediating the directed differentiation of stem cells in vitro. A new hotspot of research. Bone marrow mesenchymal stem cells can differentiate into osteoblasts, adipocytes, chondrocytes, nerve cells and smooth muscle cells. Mediating the directional differentiation of bone marrow mesenchymal stem cells into osteoblasts to provide suitable cells for transplantation is an urgent problem to be solved in the field of osteoporosis treatment in cell replacement therapy. Patent applications CN201380080663.2, CN201380080656...

Claims

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Application Information

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IPC IPC(8): C12N5/077C12N5/0775
CPCC12N5/0654C12N2506/1353C12N2537/00
Inventor 周婧
Owner 周婧
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