Bone morphogenetic protein 2 activators and application of same to promotion of osteogenic differentiation of mesenchymal stem cells

A technology of morphogenetic protein and plasmic stem cells, applied in the field of stem cells, can solve problems such as infection immune rejection

Active Publication Date: 2018-12-18
SHANDONG XINRUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection.

Method used

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  • Bone morphogenetic protein 2 activators and application of same to promotion of osteogenic differentiation of mesenchymal stem cells
  • Bone morphogenetic protein 2 activators and application of same to promotion of osteogenic differentiation of mesenchymal stem cells
  • Bone morphogenetic protein 2 activators and application of same to promotion of osteogenic differentiation of mesenchymal stem cells

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Experimental program
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Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

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Abstract

The invention relates to bone morphogenetic protein 2 activators and application of the same to promotion of the osteogenic differentiation of mesenchymal stem cells. Alkaline phosphatase (ALP) is anearly osteogenesis marker, is mainly distributed in cell membranes and can promote cell calcification. Quantitative detection of ALP can reflect the differentiation level of osteoblasts. The higher the activity of ALP is, the more obvious the differentiation of preosteoblasts into mature osteoblasts is. The high expression of ALP activity is an early indicator for the differentiation and maturation of osteoblasts. When ALP activity is enhanced, bone formation is improved and bone matrix mineralization formation is promoted. Therefore, ALP activity is a good indicator reflecting the differentiation degree and functional status of osteoblasts. To-be-tested compounds 1 to 3 promote the osteogenic differentiation of hBMSCs by activating BMP-2 protein expression in hBMSCs; and to-be-tested compounds 4 and 5 promote the osteogenic differentiation of hBMSCs by activating VEGF165 protein expression in hBMSCs.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/999C12N2506/1353
Inventor 卢珊珊赵元英
Owner SHANDONG XINRUI BIOTECH CO LTD
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