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BMP-2 activator and application of same in stem cell induced differentiation

An activator and stem cell technology, applied in the field of stem cells, can solve problems such as infection and immune rejection, achieve active proliferation, strong biological action, and promote the formation of new blood vessels

Active Publication Date: 2018-12-21
杭州爱唯生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection.

Method used

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  • BMP-2 activator and application of same in stem cell induced differentiation
  • BMP-2 activator and application of same in stem cell induced differentiation
  • BMP-2 activator and application of same in stem cell induced differentiation

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Experimental program
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Effect test

Embodiment Construction

[0016] 1. Experimental materials

[0017] The compounds to be tested are Sesquiterpene Lactone of Phyllostachys genus (to be tested 1, CAS No.: 54999-07-4), arnica lactone C (to be tested 2, CAS No.: 34532-67-7), cyclamate Analactone C (to be tested 3, CAS No.: 79383-83-8), isopalactin (to be tested 4, CAS No.: 62024-77-5), 3β,8β,5,13-bicyclic Oxygen-5,7(13)-Lactomycin-2(9),5,7( 13)-Triene-4,8-dione (under test 6, CAS No.: 135010-63-8), the structural formula is as follows figure 1 shown.

[0018] Fetal bovine serum was purchased from Thermo Company of the United States; DMEM medium was purchased from Gibco Company of the United States; ALP, CCK-8 detection kit, total protein quantitative test kit (BCA method) and RIPA cell lysate were purchased from Nanjing Jiancheng Bioengineering Institute ; BMP-2 monoclonal antibody was purchased from Beijing Zhongshan Jinqiao, and VEGF-165 monoclonal antibody was purchased from Abcam.

[0019] 2. Experimental method

[0020] 1. Isola...

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Abstract

The invention relates to a BMP-2 activator and application of the same in stem cell induced differentiation. Alkaline phosphatases (ALPs), early osteogenesis markers, are mainly distributed in cytomembranes to promote cell calcification, quantitative detection of the ALPs can reflect the differentiation level of osteoblasts, and the higher the activity is, the more obvious differentiation of preosteoblasts into the mature osteoblasts is; the high expression of the ALP activity is an early indicator for osteogenic differentiation maturation, bone formation is enhanced when the ALP activity is improved, and bone matrix mineralization is promoted, so that the ALP activity is a good indicator reflecting the differentiation degree and the functional status of the osteoblasts; to-be-tested compounds 1-3 promote osteogenic differentiation of hBMSCs by activating BMP-2 protein expression in the hBMSCs, and to-be-tested compounds 4 and 5 promote osteogenic differentiation of the hBMSCs by activating VEGF165 protein expression in the hBMSCs.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to osteogenic differentiation of stem cells. Background technique [0002] Huge bone defect has always been a common clinical problem. Bone transplantation is the main treatment method, but there are many shortcomings such as infection and immune rejection. Bone marrow mesenchymal stem cells (BMSCs) have multi-directional differentiation potential, and can differentiate into osteoblasts, chondrocytes, nerve cells and other cells under different induction conditions. Immunogenicity and the ability to differentiate into osteoblasts undoubtedly become ideal seed cells for tissue engineering. [0003] Bone morphogenetic protein 2 (BMP-2) is a multifunctional protein that can induce new bone formation and fracture healing, and plays an important role in regulating the proliferation and differentiation of stem cells during embryonic development, and can Induces osteogenic and chondrogenic diff...

Claims

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Application Information

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IPC IPC(8): C12N5/077
CPCC12N5/0654C12N2501/999C12N2506/1353
Inventor 卢珊珊赵元英
Owner 杭州爱唯生命科技有限公司
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