387 results about "Abnormal alkaline phosphatase" patented technology

The invention belongs to the technical field of biological medicine, and discloses a probiotic agent capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the probiotic agent. The invention provides a probiotic agent composed of bifidobacterium adolescentis and bifidobacterium longum, the probiotic agent can significantly improve the bone mineral density, the bone trabecula thickness, the bone trabecula number, the bone trabecula connectivity and the bone volume fraction of an osteoporosis mouse, and meanwhile, the bone trabecula separation degree of the osteoporosis-removed mouse is significantly reduced; the level of vitamin D metabolites such as 25-hydroxy vitamin D and 1, 25-dihydroxy vitamin D in mouse serum is improved; the content of serum calcium and alkaline phosphatase in serum of mice is reduced, and the level of osteocalcin and type I procollagen amino terminal peptide in the serum is improved. The probiotic agent can relieve bone calcium loss, maintain bone health, repair bone injury, increase osteoblasts and reduce the osteoporosis degree, and can be used for preparing drugs for preventing and / or treating osteoporosis or preparing health care products or food beneficial to improving bone mineral density.

The invention provides a plant lactobacillus TH13, and the preservation number of the plant lactobacillus TH13 is CGMCC (China General Microbiological Culture Collection Center) NO.32112. The plant lactobacillus TH13 provided by the invention can be used for fermenting folium cortex eucommiae and enzymatically hydrolyzed bone meal, and the fermentation product of the plant lactobacillus TH13 has high content of chlorogenic acid, proline, glycine and lysine. The fermentation product can significantly improve the content of alkaline phosphatase (ALP) and osteocalcin (OCN) in mouse serum and reduce the content of tartrate-resistant acid phosphatase (TRACP). Therefore, the fermentation product can significantly improve the bone mineral density of the femur, increase the number of bone trabecula and significantly reduce the separation degree of the bone trabecula, thereby promoting bone formation of a mouse with senile osteoporosis, inhibiting bone resorption and improving senile bone loss and bone microstructure deterioration. The preparation method of the probiotic preparation containing the plant lactobacillus and the probiotic fermentation product is simple, and large-scale production can be realized.

The invention relates to animal feed additives comprising the polypeptides having alkaline phosphatase activity and which are pH or gastric stable. The invention futher relates to the animal feed additive for preserving or maintaining the health of an animal, typically poultry or swine, including the intestinal health of the animal.

An animal feed additive comprising a polypeptide having alkaline phosphatase activity is described, wherein the selected polypeptides have activity against relevant biological targets, are thermostable, and have high three-dimensional similarity to each other and to human intestinal alkaline phosphatase and close proximity on the phylogenetic tree.

The invention relates to the technical field of in-vitro diagnostic reagent chemiluminescence detection, in particular to a Christmas liquefied carbohydrate chain antigen chemiluminescence detection kit and a preparation method thereof. The chemiluminescence detection kit comprises the following components: a KL-6 calibrator, an amino magnetic bead coupled KL-6 monoclonal antibody, an alkaline phosphatase labeled KL-6 monoclonal antibody, an AMPPD rapid chemiluminescence substrate solution and a cleaning solution, and the chemiluminescence detection kit prepared from the components is simple in detection process, easy to operate, high in automation degree and high in detection sensitivity. The kit is high in sensitivity, good in specificity and good in stability, a result can be automatically obtained within 12.5 min, the requirement for clinical auxiliary diagnosis of interstitial lung diseases can be met, and the problem that in the prior art, a chemiluminescence detection kit prepared by adopting an acridinium ester magnetic particle chemiluminescence immunoassay method or a biotin-streptavidin detection system is poor in specificity and high in sensitivity is solved. The technical problems of low marking efficiency, high manufacturing cost and poor detection result reliability in the prior art are solved.

The invention relates to a method for improving chemiluminescence immunoassay sensitivity of magnetic particles, a detection kit and application thereof, firstly, magnetic beads are coupled with an antibody, then magnetic beads are sealed to obtain a magnetic bead coated antibody, sealing comprises pre-sealing and second-step sealing, glycine and ethanolamine are added in the pre-sealing process, and in the second-step sealing process, glycine and ethanolamine are added in the second-step sealing process. Casein hydrolysate and dodecyl dimethyl amine ethyl lactone are added; then coupling the antibody with alkaline phosphatase or streptavidin to obtain an alkaline phosphatase labeled antibody or a streptavidin labeled antibody; the magnetic bead coated antibody, the alkaline phosphatase labeled antibody or the streptavidin labeled antibody is used for sample detection. The method is high in sensitivity and wide in detection range, is adaptive to a common immunoassay instrument, does not need expensive equipment, and is also simple to operate; in addition, the AMH concentration with extremely low concentration can be accurately detected.

The present technology provides antibodies specific to alkaline phosphatase, placental (ALPP) and / or alkaline phosphatase, placental-like 2 (ALPPL2), antigen-binding fragments thereof, and antibody-drug conjugates (ADCs) that can be employed as potential therapeutics for the treatment of diseases including cancer.

The invention belongs to the technical field of preparation of polypeptides, and particularly relates to a self-assembled polypeptide capable of responding to alkaline phosphatase and degrading Neuropilin-1 as well as a preparation method and application of the self-assembled polypeptide. The self-assembled polypeptide (formula I) provided by the invention has a self-assembly starting unit 'GNNQQNY (SEQ ID NO: 1)', can be self-assembled in vitro, and can form nanofibers after responding to alkaline phosphatase, so that not only can the critical self-assembly concentration of the polypeptide be reduced, but also the binding capacity with target protein can be improved. The material can degrade NRP1 on the surfaces of tumor cells and Treg cells, and has huge potential in promoting immunotherapy of tumors.

The invention relates to the field of biomedicine, particularly provides a copper-based nano-enzyme as well as a preparation method and application thereof, and aims to solve the problems that in the prior art, clinical treatment on primary sclerosing cholangitis (PSC) is difficult in diagnosis and lacks of effective treatment drugs. The copper-based nano-enzyme comprises a nano-enzyme carrier and a copper-based nano-enzyme, wherein the nano-enzyme carrier is a two-dimensional nanosheet formed by copper ions, gallic acid and ursodesoxycholic acid through coordinate bonds; the probe molecule is a cyanine dye molecule connected to the surface of the nano-enzyme carrier through a covalent bond; wherein the fluorescence intensity of the copper-based nano enzyme is enhanced after the action of the alkaline phosphatase. The three functions of alkaline phosphatase responsive diagnosis, nano-enzyme catalytic treatment and ursodesoxycholic acid hepatic targeting are innovatively and synergistically integrated into one nano platform, the treatment function can be executed while specific imaging diagnosis is performed on diseases, and a new strategy is provided for diagnosis and treatment of diseases such as PSC.

The invention discloses an antibody for detecting indol sulfate, a detection kit and application of the antibody, and the antibody can be specifically combined with indol sulfate and has high combination activity. The detection kit detects the content of indoxyl sulfate in a to-be-detected sample through a competitive binding reaction of an immunomagnetic bead coated indoxyl sulfate antibody, an antigen in the sample and an alkaline phosphatase labeled indoxyl sulfate antigen, and has important economic value and social significance.

The present invention relates to the technical field of the development and utilization of probiotics, specifically to a Weissella confusa DY12 with probiotic activity and its application. The Weissella confusa DY12 was isolated from sheep feces. The present invention identified the probiotic effect of Weissella confusa DY12 through animal experiments. The results showed that Weissella confusa DY12 could tolerate acid and bile salts and resist the gastrointestinal internal environment; its hydrophobicity was 39.47% and its auto-aggregation was 48.12%, demonstrating that the Weissella confusa DY12 of the present invention has good intestinal adhesion ability and has the potential of probiotics. The present invention verified that Weissella confusa DY12 can effectively regulate the immune function of the animal body, improve the antioxidant capacity of the body, as well as the content of lysozyme and the activity of alkaline phosphatase, and increase the daily weight gain, ultimately improving the growth performance of animals.

The invention belongs to the technical field of in-vitro diagnosis and rapid detection, and relates to an interleukin 6 and procalcitonin joint detection kit, a preparation method and a detection method, the interleukin 6 and procalcitonin joint detection kit comprises a Ra1 reagent, a Ra2 reagent, an Rb1 reagent, an Rb2 reagent and a substrate solution; the Ra1 reagent is an interleukin 6 antibody magnetic bead labeling solution, and is prepared from a biotin labeled interleukin 6 first antibody solution and a streptavidin magnetic bead solution; the Ra2 reagent is a procalcitonin antibody magnetic bead labeling solution, and is prepared from a biotin labeled procalcitonin first antibody solution and a streptavidin magnetic bead solution; the Rb1 reagent is prepared from an alkaline phosphatase labeled interleukin 6 second antibody solution; the Rb2 reagent is prepared from a second procalcitonin antibody solution labeled by horse radish peroxidase; the substrate solution comprises a first substrate solution and a second substrate solution. The detection sensitivity can be improved, the detection time is shortened, the detection efficiency is improved, and the operation is simple.

The invention discloses a method for detecting 17 beta-estradiol by using a colorimetric-fluorescent dual-mode biosensor, and belongs to the technical field of chemical analysis and detection. According to the method, a colorimetric-fluorescent dual-mode biosensor for detecting 17 beta-estradiol is established based on oxidase-like activity and fluorescence quenching characteristics of manganese dioxide nanosheets and catalytic characteristics of alkaline phosphatase by taking a nucleic acid aptamer as a recognition molecule. The synthesis cost of the nucleic acid aptamer is low, the method has universality, and other target objects can be detected by changing the nucleic acid aptamer; mBs-DNA-ALP is prepared by using a biotin-streptavidin reaction and a DNA hybridization reaction, and a structure conversion process from double-stranded DNA to an aptamer / target compound is realized by using targeted induction, so that a detection signal is amplified; according to the method for detecting 17 beta-estradiol through colorimetric-fluorescence signals, dual-mode output is achieved, results are verified and supplemented mutually, and the specificity and accuracy of the method are improved.

The disclosure features methods for treating at least one symptom of neurofibromatosis type 1, treating at least one symptom of dystrophic scoliosis, or inducing bone healing by administering a soluble alkaline phosphatase (sALP) to the patient.

The invention discloses a fluorescence sensor for detecting thromboregulatory protein as well as a preparation method and a use method of the fluorescence sensor. The preparation method comprises the following steps: S1, adding a capture antibody of the thromboregulatory protein into an elisa plate; s2, adding the prepared bovine serum albumin; s3, adding prepared thrombus regulatory protein solutions with different concentrations; s4, adding a prepared detection antibody modified with alkaline phosphatase and used for detecting thromboregulatory protein; s5, adding a p-nitrophenyl disodium phosphate solution, and fully reacting; s6, a NO2-Eu-MOF nanosheet solution is prepared; s7, preparing a 1, 4-benzene diphosphonic acid solution; s8, adding a 1, 4-benzene diphosphonic acid solution into the NO2-Eu-MOF nanosheet solution, and detecting a fluorescence signal; and S9, adding the solution obtained in the step S8 into the solution obtained in the step S5, detecting the fluorescence intensity, and constructing a fluorescence intensity-concentration working curve. According to the invention, the concentration of thromboregulatory protein in the sample can be quantitatively analyzed.

Featured are polypeptides that include soluble alkaline phosphatases, mutants, fragments, fusion proteins thereof, and methods of use thereof, for treating bone mineralization disorders, such as hypophosphatasia (HPP), and symptoms thereof. The polypeptides include a soluble alkaline phosphatase (sALP) or fragment thereof, which is derived from a naturally occurring alkaline phosphatase (ALP).

The invention discloses an eggshell membrane peptide for enhancing bone mineral density as well as a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of the eggshell membrane peptide for enhancing bone mineral density, which comprises the following steps: carrying out vacuum freeze drying and crushing on an eggshell membrane to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernate; adding collagenase, carrying out enzymolysis, and centrifuging to take supernate, so as to obtain primary enzymatic hydrolysate; adding alkaline phosphatase, performing enzymolysis and centrifugation, and taking supernate to obtain enzymatic hydrolysate; and performing ultrafiltration and drying to obtain the eggshell membrane peptide. According to the eggshell membrane peptide for enhancing the bone mineral density, keratin cross-linking is destroyed through enzymolysis of keratinase, active peptide capable of stimulating osteoblast proliferation is released through enzymolysis of collagenase, phosphopeptide calcium binding sites are further activated through enzymolysis of alkaline phosphatase, calcium salt deposition is promoted, and the eggshell membrane peptide for enhancing the bone mineral density is provided.

The invention relates to a protein with alkaline phosphatase activity and application thereof. In particular to a protein with alkaline phosphatase activity, a gene for coding the protein, a recombinant vector inserted with the gene, a transformant transformed with the gene, a method for preparing alkaline phosphatase and application of the alkaline phosphatase in preparation of high-temperature-resistant and / or alkali-resistant products. The protein with alkaline phosphatase activity disclosed by the invention has relatively high high temperature resistance and alkali resistance, and lays a foundation for improving the application of alkaline phosphatase in the fields of food, feed, medicine, environmental governance, pesticide degradation and the like.

The invention discloses a nanofiber based on a polypeptide derivative and an enzymatic self-assembly method and application of the nanofiber based on the polypeptide derivative, the sequence of the polypeptide derivative is Nap-Gly-Phe-Phe-pTyr-Lys-Trp-Tyr-Gln-Asn-Met-Ile-Arg, and the Gly-Phe-Phe-pTyr is of an L configuration or a D configuration. The self-assembled polypeptide derivative disclosed by the invention contains an alkaline phosphatase restriction enzyme cutting site, and the self-assembled polypeptide derivative is self-assembled at a specific part with high enzyme specificity expression through enzyme catalysis to form nanofibers. The nanofiber hydrogel obtained through enzymatic treatment is carried out under physiological conditions, high temperature or ultraviolet irradiation is not needed, damage of high temperature to bioactive substances is avoided, the nanofiber hydrogel is more suitable for a temperature-sensitive in-vivo environment, toxic chemical cross-linking agents or photoinitiators do not need to be introduced, the cytotoxicity of the material is remarkably reduced, active enzymes or drugs can be embedded in situ, and the nanofiber hydrogel can be used for in-situ treatment. The biological function is maintained.

The invention discloses a probe set, a kit and a nucleic acid signal amplification in-situ hybridization detection method, and relates to the technical field of molecular biology, the probe set comprises: a labeled extension probe, the sequence of which is as shown in SEQ ID No.9-24; and the sequence of the closed probe is as shown in SEQ ID No. 25-27. The kit comprises the probe set, a signal amplification precursor, a signal amplification body and an alkaline phosphatase labeled probe. According to the present invention, the partial probe sequence introduces the non-natural bases, and can be used for detecting the HPV18 infected cervical cancer Hela cells, the experiment results show that the introduction of the non-natural bases does not affect the normal positive value, the matching degree between the non-natural bases is more specific than the matching of the natural bases, and the detection sensitivity is improved.

The present invention discloses a type I semi-cyanine photosensitizer, a type I targeted photosensitizer, and a preparation method and application thereof. The semi-cyanine photosensitizer can rapidly generate a large amount of O2 under light and hypoxic conditions. ·‑ , thereby achieving the effect of photodynamic therapy, and having good biocompatibility and biodegradability, it is an effective type I photosensitizer. In addition, the above-mentioned type I photosensitizer is reacted with phosphorus oxychloride in the presence of water to prepare a type I photosensitizer with tumor targeting. It can specifically target alkaline phosphatase, a biomarker highly expressed in tumors. Under the action of alkaline phosphatase, the photosensitizer can be enriched in the tumor area, thereby achieving precise tumor imaging and photodynamic therapy, which can effectively reduce the side effects of the photosensitizer and improve the inhibitory effect of the photosensitizer on tumors.

The invention relates to a dog early pregnancy RLN detection kit and a detection method thereof. The kit comprises a magnetic bead working solution, an enzyme working solution, a cleaning solution, a chemiluminescent substrate, a calibration product and a quality control product. Wherein the magnetic bead working solution contains toluenesulfonyl magnetic beads labeled by canine RLN monoclonal antibodies, and the enzyme working solution contains canine RLN monoclonal antibodies labeled by alkaline phosphatase and an antibody blocking agent. When the canine early pregnancy RLN detection kit provided by the invention is used for determining the canine RLN concentration in serum, the RLN antibody on the magnetic bead and the RLN antibody on the enzyme marker are simultaneously and rapidly combined with different sites on the RLN antigen to form an antibody-antigen-antibody structure, the combination is more sufficient, the detection sensitivity is high, the antibody blocking agent shortens the window period of detection, and the detection time is shortened. The false positive of the detection result is reduced, and the detection accuracy is improved.

The invention discloses bifidobacterium longum subsp.longum capable of improving the physiological activity function of vitamin D and maintaining bone health and application of the bifidobacterium longum subsp.longum, and belongs to the technical field of biological medicine. The bifidobacterium longum subsp. Longum CCFM1448 provided by the invention can obviously improve the total bone mineral density, the bone mineral density of distal femur bone trabecula, the thickness of the bone trabecula, the number of the bone trabecula, the connectivity of the bone trabecula and the bone volume fraction of an osteoporosis mouse, and obviously reduce the separation degree of the bone trabecula of the osteoporosis mouse; the content of a vitamin D metabolite 1, 25-dihydroxy vitamin D in serum of a mouse with osteoporosis is remarkably increased, the content of serum calcium and alkaline phosphatase in the serum of the mouse with osteoporosis is reduced, and the level of osteocalcin and I-type procollagen amino-terminal original peptide in the serum is increased. Therefore, the bifidobacterium longum subsp. Longum CCFM1448 has the effects of relieving bone calcium loss, maintaining bone health, repairing bone injury, increasing osteoblasts and reducing osteoporosis degree.

The invention relates to the field of phosphorylated tau protein detection, in particular to a method for improving phosphorylated tau protein detection sensitivity and a kit thereof. The invention provides a method for improving phosphorylated tau protein detection sensitivity. The method comprises the following steps: S101, obtaining a blood sample; s102, adding the detection reagent into the blood sample; s103, detecting the concentration of the phosphorylated tau protein in the blood sample by using a chemiluminescence method; wherein the detection reagent comprises an alkaline phosphatase polymer-antibody compound. The alkaline phosphatase polymer-antibody compound prepared by the invention is high in yield, good in stability, high in coupling efficiency, low in background, good in precision, strong in anti-interference capability, smaller in batch difference and lower in non-specific adsorption, the sensitivity is remarkably improved, and the compound shows remarkable advantages in detection of phosphorylated tau protein in a blood sample.

The invention discloses a nano-enzyme, a detection kit containing the nano-enzyme and application of the nano-enzyme in heavy metal detection. The zirconium-doped cerium dioxide nano-enzyme is prepared by a chemical synthesis method, and has the activity of alkaline phosphatase. The nano-enzyme can catalyze hydrolysis of a substrate to generate chemiluminescence, so that the nano-enzyme is applied to the technical field of chemiluminescence analysis and detection, and the problems that natural enzyme is easily influenced by the external environment and is poor in stability are solved. The invention also discloses a chemiluminescence immunodetection kit for detecting heavy metals. The kit realizes automatic quantitative analysis of heavy metals based on the zirconium-doped cerium dioxide nano-enzyme and antigen-antibody specific binding property, has good specificity and sensitivity, and effectively reduces the heavy metal detection cost.

The invention discloses a synthesis method of an amino-acid-based MOFs (Metal-Organic Frameworks) derived nano-enzyme, and relates to the technical field of nano-enzyme synthesis, the synthesis method of the amino-acid-based MOFs derived nano-enzyme comprises the following steps: step 1, selecting proper metal ions and organic ligands; step 2, ultrasonic dispersion; step 3, precipitate drying; and step 4, activating treatment of the nano enzyme. The MOFs-derived nano-enzyme with alkaline phosphatase activity is synthesized by taking natural amino acid as a ligand and one of ions of zirconium, copper and iron as a metal node and introducing defects by a method of adding an acidic regulator in synthesis, and the crystal structure of the nano-enzyme is not changed under the introduction of the defects, so that the alkaline phosphatase activity of the MOFs-derived nano-enzyme is improved. The MOFs-derived nano-enzyme has the advantages that the MOFs-derived nano-enzyme is modified, the catalytic hydrolysis activity of the MOFs-derived nano-enzyme on phosphate substrates is greatly improved, good substrate specificity is shown, the structure of the MOFs-derived nano-enzyme does not change after long-term incubation under acidic, alkaline and high-temperature conditions, and compared with natural enzyme, the MOFs-derived nano-enzyme has good stability, pH stability and reusability.

The invention relates to a liver cirrhosis prediction and noninvasive diagnosis system and method for autoimmune hepatitis, and belongs to the technical field of medicine. The system comprises a data acquisition module, a data processing module and a model construction module. The data acquisition module is used for acquiring blood detection indexes of a patient, including total protein, albumin, total bilirubin, direct bilirubin, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), glutamyltranspeptidase (GGT), urea, creatinine, platelet count (PLT) and the like. The model building module is combined with blood detection data and clinical pathology data, a statistical analysis method such as binary Logistic regression is adopted, and a liver cirrhosis prediction model is trained and built. Based on four key blood detection indexes, the method can effectively predict the occurrence of liver cirrhosis of AIH patients, and especially provides a scientific reference for noninvasive diagnosis and curative effect monitoring for patients who cannot perform liver puncture or reject liver puncture.

The invention provides a multicolor visual semi-quantitative detection method for miRNA in skin interstitial fluid, which comprises the following steps: extracting a skin interstitial fluid sample in a minimally invasive manner by combining a microneedle patch with a vacuum negative pressure technology; the method comprises the following steps: incubating a skin interstitial fluid sample and a magnetic nanoprobe of which the surface is immobilized with a hairpin probe H1, opening H1 in the presence of target miRNA, triggering a subsequent hybridization chain reaction of a hairpin probe H2 and H3, and constructing a biotin-rich DNA compound on the surface of a magnetic bead; the method comprises the following steps: carrying out enzyme labeling by using an alkaline phosphatase-streptavidin conjugate, catalyzing ascorbic acid phosphate to generate ascorbic acid to reduce potassium iodate, and generating an iodine elementary substance to etch a gold nano bipyramid, so that the color of a solution is changed, and high-sensitivity detection on target miRNA is realized. The multi-color visual detection method provided by the invention is combined with the gold nano bipyramid localized surface plasmon resonance property change, so that near-full spectrum color change can be generated, and the portability of the sensor and the accuracy of visual analysis can be improved.

The invention discloses an SERS (Surface Enhanced Raman Scattering) probe for monitoring a redox reaction as well as a preparation method and application of the SERS probe. A metal complex TEPE-Au is prepared firstly, and the chemical structural formula of the metal complex TEPE-Au is shown as a formula I; the TEPE-Au complex has an obvious Raman characteristic peak in a silent zone, shows high sensitivity and high resolution, and can be used as a silent zone reporter molecule. After being prepared into an SERS probe, the SERS probe is combined with an SERS detection technology, so that the SERS probe can be used for monitoring redox reaction, can also be used for detecting the concentration or content of alkaline phosphatase, and has a good application prospect in the fields of biological detection, chemical detection or medical diagnosis and the like. And the formula I of the # imgabs0 # is shown in the specification.

The invention provides an alkaline phosphatase color developing solution and a preparation method thereof, and particularly relates to the technical field of immunodetection, the alkaline phosphatase color developing solution is formed by dissolving chromogen dry powder in a color developing buffer solution, and the concentration is 1-3 mg / ml; wherein the chromogen dry powder is composed of naphthol AS-MX phosphate and fast red-TR; the preparation method comprises the following steps: S1, dissolving naphthol AS-MX phosphate and fast red TR with purified water to obtain a solution; wherein the concentration of naphthol AS-MX phosphate in the solution is 0.5 to 1.5 mg / ml, and the concentration of fast red TR is 0.5 to 1.5 mg / ml; s2, freezing and storing the solution in a refrigerator, and condensing the solution into ice blocks; s3, the ice blocks are put into a freeze dryer, vacuumizing is carried out, water molecules in the ice blocks are sublimated into water vapor under the negative pressure condition, the water vapor is discharged, and chromogen dry powder can be obtained; s4, the chromogen dry powder is dissolved in the chromogenic buffer solution, and an alkaline phosphatase chromogenic solution can be obtained; the prepared developing solution has the core advantages of high sensitivity, rapid developing, multicolor compatibility, digital adaptation and the like, and an efficient and reliable tool is provided for pathological research and clinical diagnosis.