Chemiluminescence quantitative detection kit for procalcitonin, and preparation method and detection method thereof
A procalcitonin and chemiluminescence technology, applied in the field of immunoassay, can solve the problems of low sensitivity of the kit, narrow detection range, poor specificity of the kit, etc., and achieves simple and time-saving operation, wide detection range and good accuracy Effect
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Embodiment 1
[0038] Embodiment 1: the preparation of kit
[0039] (1) Preparation of magnetic separation reagents:
[0040] Materials and Instruments:
[0041] 1. Magnetic particles: the surface contains carboxyl (COOH) active groups, and the carboxyl group content per gram of magnetic particles (dry weight) is 0.4 mmol, with superparamagnetism and a diameter of 1 μm.
[0042] 2. Streptavidin was purchased from Sigma.
[0043] 3. 2-Morpholineethanesulfonic acid (MES), carbodiimide (EDC), trishydroxymethylaminomethane (TRIS) and other reagents are all chemically pure.
[0044] 4. Analytical balance
[0045] Steps:
[0046] 1. Take 100mg of magnetic particles, magnetically separate to remove the supernatant, and resuspend in 10ml of 0.05mol / L, pH4.7 MES buffer;
[0047] 2. Add 3 mg of streptavidin and suspend at room temperature for 50 minutes;
[0048] 3. Add 0.7ml freshly prepared 10mg / ml EDC aqueous solution and suspend at room temperature for 8h;
[0049] 4. Magnetic separation, r...
Embodiment 2
[0081] Embodiment 2: the implementation of detection and the evaluation of detection effect
[0082] Materials and Instruments:
[0083] 1. The kit prepared by Example 1;
[0084] 2. The luminescent substrate and cleaning solution are produced by Suzhou Hao Oubo Bio-Pharmaceutical Co., Ltd.
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