Chemiluminescence quantitative detection kit for procalcitonin, and preparation method and detection method thereof
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A procalcitonin and chemiluminescence technology, applied in the field of immunoassay, can solve the problems of low sensitivity of the kit, narrow detection range, poor specificity of the kit, etc., and achieves simple and time-saving operation, wide detection range and good accuracy Effect
Active Publication Date: 2014-07-02
SUZHOU HAOOUBO BIOPHARML
View PDF2 Cites 39 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
However, the sensitivity of the kit in this patent is still low (0.018ng / ml), the specificity of the kit is also poor, and the detection range is narrow
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0038] Embodiment 1: the preparation of kit
[0039] (1) Preparation of magnetic separation reagents:
[0040] Materials and Instruments:
[0041] 1. Magnetic particles: the surface contains carboxyl (COOH) active groups, and the carboxyl group content per gram of magnetic particles (dry weight) is 0.4 mmol, with superparamagnetism and a diameter of 1 μm.
[0042] 2. Streptavidin was purchased from Sigma.
[0043] 3. 2-Morpholineethanesulfonic acid (MES), carbodiimide (EDC), trishydroxymethylaminomethane (TRIS) and other reagents are all chemically pure.
[0044] 4. Analytical balance
[0045] Steps:
[0046] 1. Take 100mg of magnetic particles, magnetically separate to remove the supernatant, and resuspend in 10ml of 0.05mol / L, pH4.7 MES buffer;
[0047] 2. Add 3 mg of streptavidin and suspend at room temperature for 50 minutes;
[0048] 3. Add 0.7ml freshly prepared 10mg / ml EDC aqueous solution and suspend at room temperature for 8h;
[0049] 4. Magnetic separation, r...
Embodiment 2
[0081] Embodiment 2: the implementation of detection and the evaluation of detection effect
[0082] Materials and Instruments:
[0083] 1. The kit prepared by Example 1;
[0084] 2. The luminescent substrate and cleaning solution are produced by Suzhou Hao Oubo Bio-Pharmaceutical Co., Ltd.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
concentration
aaaaa
aaaaa
diameter
aaaaa
aaaaa
concentration
aaaaa
aaaaa
Login to view more
Abstract
The invention relates to a chemiluminescence quantitative detection kit for procalcitonin, and a preparation method and detection method thereof. The kit comprises a procalcitonin series standard substance, a magnetic separation reagent (magnetic particle suspension coupled with streptavidin), a first reagent (anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label) and a second reagent (anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label). The sensitivity of the kit prepared from the magnetic particle suspension coupled with streptavidin, anti-procalcitonin monoclonal antibody solution containing biotin N-hydroxysuccinimide ester label and anti-procalcitonin monoclonal antibody solution containing alkaline phosphatase label is up to 0.008ng/ml; and the kit has the advantages of high accuracy, high precision, no need of prediluting the sample, and wide detection range, and is simple and time-saving to operate.
Description
technical field [0001] The invention relates to the field of immune analysis, in particular to a chemiluminescence quantitative detection kit for procalcitonin, a preparation method and a detection method thereof. Background technique [0002] Procalcitonin (PCT) is derived from a single-copy gene located on chromosome 11 (11p15,4), which consists of 280 base pairs with 6 exons and 5 introns. After transcription, it is translated into Preprocalcitonin in the rough endoplasmic reticulum of parafollicular cells of the thyroid, including 84 N-terminal amino acids, active calcitonin and calcitonin. The procalcitonin precursor cuts off the single sequence of nPro-CT end under the action of endogenous polypeptide enzymes to generate 116 amino acid PCT with a molecular weight of about 13kD. PCT and calcitonin have the same 32 amino acid sequence (60~ 91 bits). [0003] PCT is expressed by neuroendocrine cells (including C cells in thyroid, lung, and pancreatic tissues) and is cle...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more