192 results about "Antibodies monoclonal" patented technology

The present invention relates to conjugates of therapeutically useful anthracyclines with carriers such as polyclonal and monoclonal antibodies, proteins or peptides of natural or synthetic origin; methods for their preparation, pharmaceutical composition containing them and use thereof in treating certain mammalian tumors.

This invention relates to a monoclonal antibody capable of combining with H5 subtype avian influenza virus HA protein specifically, the hybridoma cell line secreting said antibody and a preparing method. The invention also relates to a serial test kit for testing H5 subtype avian influenza virus by the antibody and a bit of said antibody in the test sample of the virus and its usage in treatment.

The present invention relates to methods for analyzing protein samples, e.g., monoclonal antibodies, via the SITRS technique, as well as methods and systems that facilitate the processing of data generated by the SITRS technique.

The invention discloses an anti-rabies virus monoclonal antibody and a preparation method and an application, belonging to the field of biomedicine and particularly relating to the preparation of a monoclonal antibody capable of identifying rabies virus and the application. The monoclonal antibody of the invention is screened by indirect Enzyme-linked immunosorbent assay (ELISA), and specificity and affinity thereof combined with antigen are identified by methods such as polyacrylamide gel electrophoresis analysis, speckle ELISA, immunoblot analysis and the like. The anti-rabies virus monoclonal antibody of the invention can be applied in multiple testing methods of antigen of rabies virus and can be also applied in the preparation of rabies virus detecting kit. The anti-rabies virus monoclonal antibody is secreted by anti-rabies virus monoclonal antibody hybridoma cell strain 2C5 with the preservation number being CGMCC No.3014.

The invention discloses an anti-avian leukosis virus p27 protein monoclonal antibody, a gold-colloidal strip containing the same, and application. The anti-avian leukosis virus p27 protein monoclonal antibody is generated by secretion of a hybridoma cell strain with a preservation number of CGMCC NO. 11089 or a hybridoma cell strain with a preservation number of CGMCC NO. 11090. The monoclonal antibody is high in titer to p27 protein and good in specificity, and therefore can be used for preparing a kit and the gold-colloidal strip for detecting an avian leukosis virus. A preparation method of the monoclonal antibody provided by the invention is simple, and an antibody purification process is simple, and the efficiency is high, and the cost is low. As the gold-colloidal strip prepared by the anti-avian leukosis virus p27 protein monoclonal antibody provided by the invention is adopted to detect the anti-avian leukosis virus, the specificity is strong, and the operation is simple, and convenience, speediness and simplicity are realized, besides, a special instrument and equipment are not needed, and professional training is not needed, and a result is clear and easy to recognize; the operation is simple and convenient; the popularization is easy, and the antibody is more suitable for real-time detection.

The present invention provides compositions and methods for inhibiting the growth of a GITR-expressing cancer cell which cells may include, but are not limited to cells of epithelial origin such as NSCLC, prostate cancer, breast cancer, colon cancer and ovarian cancer and to treat or ameliorate the symptoms associated with the presence of these cells in a subject. Suitable compositions for use in these methods are antibodies that selectively recognize and bind to GITR (Glucocorticoid-induced TNFR-related protein) present on these cancer cells. The antibodies can be either polyclonal or monoclonal antibodies.

The invention relates to the research field of biological diagnostic reagents, in particular to an ELISA (Enzyme-Linked Immunosorbent Assay) kit for detecting APP (Actinobacillus Pleuropneumoniae). The kit comprises a washing solution, a colorimetric solution, a stopping solution, a positive control specimen, a negative control specimen and an ELISA plate coating a first antibody as well as a second antibody for detection, wherein the first antibody is a monoclonal protein antibody of the APP rApxIVN, and the second antibody is a biotin-labeled monoclonal protein antibody of the APP rApxIVN. The invention can be applied to detecting APP-infected pig serum and has favorable importance and sensitivity.

The invention relates to an aqueous two phase extraction (ATPE) augmented precipitation process, which may be used to recover and also partially purify therapeutic proteins, including monoclonal antibodies from a crude multi-component mixture. The process involves the formation of a forward extraction PEG-Phosphate ATPE system in which the target product is preferentially partitioned to the polymer rich phase. A second ATPE back extraction system is then formed by introducing the polymer rich phase from the forward extraction to a new phosphate salt rich phase, causing the product to precipitate at the interface between the two phases. This precipitate is then recovered and resolubilised in a suitable buffer and may be passed on for further purification.

Monoclonal antibodies that bind and inhibit biological activities of human FGFR2 are disclosed. The antibodies can be used to treat cell proliferative diseases and disorders, including certain forms of cancer, associated with activation or overexpression of FGFR2.

The embodiments of the invention relate to the field of immunoassay, and in particular to a human heparin binding protein assay kit with high sensitivity and wide detection range. The human heparin binding protein assay kit comprises: a reagent 1, a reagent 2 and human HBP calibrators with different concentration gradients. The reagent 2 comprises a carboxyl latex microsphere labeled only with human HBP monoclonal antibody and a carboxyl latex microsphere labeled only with human HBP polyclonal antibody. The average particle diameter of the carboxyl latex microsphere labeled only with the humanHBP monoclonal antibody > the average particle diameter of the carboxyl latex microsphere labeled only with the human HBP polyclonal antibody. The human heparin binding protein assay kit provided bythe invention can complete a single sample test within 10 minutes, and indexes of precision, accuracy and anti-interference are excellent. The invention can be used clinically to predict an organ dysfunction caused by sepsis, and can be used as an early diagnostic marker for sepsis, especially serious bacterial infection.

The provided human epididymis specific express protein ESC42 with coded sequence can be prepared by chemical method or genetic engineering technique, and can be used to prepare antibacterial drug or new contraceptive.

The invention discloses a neutralizing monoclonal antibody resisting to tetanus toxin. Hybridoma cells are obtained from tetanus toxin heavy chain C fragment immune mice, light chain and heavy chain variable region genes of the antibody are taken, human-mouse chimeric complete antibody expression vectors are built, CHO cell transfection is carried out, purification is carried out, and then the antibody is obtained. The antibody has the activity of specific binding to tetanus toxin, the monoclonal antibody can partially protect mice against attack of tetanus toxin, and four antibodies can completely protect mice against a twofold lethal dose of tetanus toxin in combination.

The invention discloses a porcine pseudorabies virus gE IgM antibody colloidal gold immunochromatograohic assay test strip as well as a preparation method and application thereof. The test strip comprises a sample absorption region, a colloidal gold labeled probe region, a solidifying antibody region, a water absorption region, a bottom plate and a clamping shell, wherein the sample absorption region, the colloidal gold labeled probe region, the solidifying antibody region and the water absorption region are sequentially bonded on the bottom plate and are interlapped in the clamping shell; the sample absorption region is coated with purified porcine pseudorabies virus gE protein; the colloidal gold labeled probe region is coated with colloidal gold labeled porcine pseudorabies virus gE protein monoclonal antibody; the solidifying antibody region is sequentially provided with a detection line T coated with mouse anti-pig IgM monoclonal antibody and a control line C coated with goat anti-mouse IgG. The test strip is simple to operate, good in repeatability, high in sensitivity, rapid and intuitive in result, simple in process and low in cost, can be mass-prepared, and is suitable for mass field test and early diagnosis for porcine pseudorabies virus infection in swinery.

The invention discloses a high-specificity and high-affinity anti-Pp65 monoclonal antibody which is generated by a hybridoma cell strain 8D6 of the anti-Pp65 monoclonal antibody, wherein the cell strain 8D6 is collected in China Center For Type Culture Collection (CCTCC) and has a collection number of CCTCC NO:C201476. The monoclonal antibody secreted by the hybridoma cell strain has good affinity and specificity, and the immunoreactivity and the specificity of the antibody are superior to those of domestic and imported monoclonal antibodies. The anti-Pp65 monoclonal antibody can serve as a key raw material for blood white cell immunofluorescent cytochemical diagnosis and immunofluorescent quantitative PCR (polymerase chain reaction) detection of HCMV (human cytomegalovirus) infection, also can serve as a key raw material for detecting HCMV by using a double antibody sandwich method, has important meaning for establishment of a quick and sensitive clinical diagnosis reagent to HCMV infection, and has important application prospects.

The invention discloses an epididymal protease inhibitor protein monoclonal antibody and application thereof, which belong to the field of biological engineering. The monoclonal antibody is secreted by hybridoma preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of C200849. The monoclonal antibody can be combined to the epididymal protease inhibitor protein Eppin protein of specific expression in a testis and epididymis tissue by specificity. The monoclonal antibody can be used for preparing male infertility diagnostic reagents, male contraceptives and anti-fertility study biological products.

The invention provides a kit for improving GPC3 detection sensitivity and a preparation method thereof, and belongs to the technical field of immunodetection; the kit comprises a reagent M and a reagent R; the reagent M is prepared by diluting a magnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody, and the diameter of the magnetic bead particle is 0.8-3[mu]m; the diluent of themagnetic bead particle coupled mouse anti-human GPC3 monoclonal antibody comprises the following components: Procline 300, a buffer solution, a surfactant and protein; and the reagent R is prepared by diluting a rabbit anti-human GPC3 polyclonal antibody marked by alkaline phosphatase. The kit has the advantages of higher GPC3 detection sensitivity and wider linear range. The invention also provides a preparation method of the kit for improving the GPC3 detection sensitivity.