84results about How to "Meet the needs of on-site testing" patented technology

The invention relates to a rapid immunomagnetic bead chromatographic method for main allergens in aquatic products. A rat monoclonal antibody which has specific reaction with a tropomyosin (Tm) allergen of edible crustacean aquatic organisms and edible soft-bodied aquatic organisms, has specific reaction with a parvalbumin (Pa) allergen of fishes, frogs, rats and sea turtle vertebrates, and is prepared and screened by hybridoma technology is taken as a specific antibody and coupled to the surface of a magnetic nanometer material in a chemical bonding mode to serve as a marker so as to prepare a specific nanometer detection probe suitable for magnetic bead chromatography; the allergen specific nanometer detection probe and sample solution are added into a sample pad of an allergen magnetic immunochromatographic test strip, the sample pad is stood at room temperature for 20 minutes, the allergens are captured by a detection strip and a control strip through chromatography to form a macroscopic colored strip, so that immunochromatography is realized; and a test card is put on a magnetic assay reader, the content of the allergens in a sample to be detected is calculated according to a marked line, comparison is performed and the immunochromatography is finished.

The invention discloses a bacteria drug-resistance detection system and an operation method thereof. The system comprises a drug sensitive test inoculation instrument, drug sensitive test image acquisition conversion equipment, a drug sensitivity detection kit and a bacterial strain culture device. A medium is arranged in the drug sensitivity detection kit. The drug sensitive test inoculation instrument is used for inoculating a bacterial strain into the drug sensitivity detection kit. The bacterial strain culture device provides a growth environment for the bacterial strain in the drug sensitivity detection kit. The drug sensitive test image acquisition conversion equipment is used for acquiring and converting images of the bacterial strain in the drug sensitivity detection kit. Management software is arranged in the drug sensitive test image acquisition conversion equipment, and the management software is connected to the network. With combination of online and offline, clinical medication is directly guided. The system meets on-site detection requirements, is convenient for data analysis and decision-making for a farm, a veterinary medicines factory and an administrative department, is suitable for current high-level drug resistance situation in our country, and satisfies the need of quantitative networked monitoring of drug resistance. A broth dilution method and an agar dilution method are both considered. The system meets different requirements of large-scale monitoring and scattered sample detection.

The invention provides a multi-component content prediction method under the condition of coexistence of rare earth ions with color characteristics and without color characteristics, and relates to the field of component content prediction in the rare earth extraction process. The method comprises: the problem that the component content is difficult to quickly and accurately detect exists in the rare earth extraction process; the GA-ELM-based rare earth extraction process multi-component content prediction method is provided for solving the problem that the original color feature-based singlerare earth element component content detection method is not applicable due to the fact that the image color features of a CePr/Nd mixed solution containing colorless Ce ions are greatly different from those of a Pr/Nd solution. The method comprises the following steps: firstly, searching H and S components with the maximum correlation with the component content in an HSI color space; secondly, establishing a multi-component content soft measurement model based on an extreme learning machine ELM by taking H and S component first moments as input; for the uncertainty of an initial weight and athreshold value of an ELM model, using a genetic algorithm GA to optimize model parameters, so that the precision of the optimized ELM model with the component content is higher.

The invention relates to the technical field of immunodiagnosis, and discloses colloidal gold immunochromatography test paper for combined diagnosis of COVID-19 and mycoplasma pneumoniae aiming at theblank problem of COVID-19 and mycoplasma pneumoniae antibody detection in the prior art, which specifically comprises the following preparation steps: 1) preparing a detection line T and a quality control line C; 2) preparing a colloidal gold conjugate treatment pad; and 3) preparation of test paper: sequentially connecting and assembling the water absorption pad, the sample pad, the colloidal gold conjugate treatment pad, the antibody-coated modified nitrocellulose membrane and the PVC bottom plate to obtain a finished product. The prepared test paper can accurately detect whether a person has 2019-nCoV and mycoplasma pneumonia or not, is short in detection period, simple in detection process and portable in equipment, adopts an antibody antigen detection mode as the Antigen has no latent period, and the invention is visual and accurate in result display and high in specificity, can diagnose infection of new crowns and pulmonary branches at different time, and is high in detection efficiency.

The invention discloses a canine parvovirus colloidal gold immunochromatography test strip and a preparation method. The test strip comprises a sample absorbing area, a gold label probe area, an immobilized antibody area, a water absorbing area and a bottom plate, wherein the sample absorbing area, the gold label probe area, the immobilized antibody area and the water absorbing area are sequentially paved on the bottom plate and partially overlapped each other; the gold label probe area is coated with a colloidal gold labeled anti-canine parvovirus monoclonal antibody F1; the immobilized antibody area is sequentially provided with a test line and a control line, the test line is coated with an anti-canine parvovirus monoclonal antibody B6, and the control line is coated goat anti-mouse IgG (immunoglobulin G). According to the preparation method, a glass fiber membrane, a polyester membrane coated with the anti-canine parvovirus monoclonal antibody F1, a nitrocellulose membrane coated with the test line and the control line, and water absorbing filter paper are assembled onto a polyethylene plate to obtain the canine parvovirus colloidal gold immunochromatography test strip. The test strip is fast to test, high in accuracy rate, high in specificity, and simple and convenient to carry and operate.

The invention relates to an anti-rabies virus IgG antibody colloidal gold immunochromatographic assay reagent plate and a preparation method. Glass fiber paper and cellulose nitrate films are laid on a polyethylene plate and a polyvinyl chloride substrate film; the cellulose nitrate film is coated with an assay line and a contrast line; and a gold-labeled probe polyester film is adhered on the assay line side; water-absorbing filter paper is adhered on the contrast line side, wherein the assay line is coated with rabies virus purified antigen, and the contrast line is coated with anti-SPA purified antigen. The reagent plate of the invention has the advantages of rapid detection, high detection accuracy, high specificity, convenient carrying, simple and convenient operation and can be usedfor detecting rabies virus antibodies of various animals and human. The assay reagent plate can be stored at the normal temperature for 1 year without special equipment or apparatus and has high assay repeatability.

The present application discloses a PEDV immuno-chromatography test strip along with preparation method and application. The PEDV immune-chromatography test strip of the present application comprises:the sample pad and the chromatography strip on support material; the assay band coated with anti-PEDV antibody; the quality control band coated with goat anti-mouse IgG antibody; and the sample pad sprayed with quantum dot-labeled anti-PEDV antibody probe. The PEDV immuno-chromatography test strip of the present application employed quantum dot-labeled anti-PEDV antibody probe for bioassay. It combines the high sensitivity of fluorescent quantum dots and the high specificity of antigen and antibody, so that the immuno-chromatography test strip has the advantages of high sensitivity, good specificity and short testing period. It is good for on-site test and is suitable for screening and monitoring the porcine epidemic diarrhea virus.

The invention discloses a dynamic sounding method for detecting a compaction degree by replacing a sand replacement method with foundation bearing capacity. The dynamic sounding method comprises the steps of manufacturing a steel mould with the inside diameter of 200 mm and the height of 350 mm through a mould manufacturing technology; calculating the optimal water content and the maximum dry density through an indoor compaction test; performing static pressure molding with a press machine according to the optimal water content and the maximum dry density of soil as well as the specified compaction degree, and then selecting light or heavy dynamic sounding according to the soil types; then, detecting a test piece according to the dynamic sounding method of the foundation bearing capacity to obtain the indoor data; performing detection by replacing the sand replacement method with the dynamic sounding method on site, if the result is no lower than the indoor detection data, the compaction degree is qualified; the detection data in the dynamic sounding method disclosed by the invention is more comparable and reliable, so that the result is closer to the true value; it is more important that the detection efficiency is improved greatly; the detection speed is high, and the data is more accurate; the field detection requirements and the actual progress requirements of a construction site are met, and detection counterfeiting is avoided; and the cost is reduced greatly.

The invention discloses detection test paper for quickly diagnosing the Lyme disease, and a preparation method thereof. A method for detecting the Lyme disease by ELISA (Enzyme Linked Immunosorbent Assay) is long in detection time and is not suitable for substrate detection. The colloidal gold immunochromatography detection test paper for quickly diagnosing the Lyme disease comprises a bottom plate, a sample cushion, a Jinbiao cushion, a nitrocellulose membrane and a water adsorption cushion, wherein the sample cushion, the Jinbiao cushion, the nitrocellulose membrane and the water adsorptioncushion are arranged and connected in sequence and are all arranged on the bottom plate; the nitrocellulose membrane is provided with a first detection line, a second detection line and a quality control line, wherein the first detection line is provided with a mouse anti-human IgM (Immunoglobulin M) monoclonal antibody, the second detection line is provided with a mouse anti-human IgG (Immunoglobulin G) monoclonal antibody, and the quality control line is provided with a goat anti-mouse IgG polyclonal antibody; and the Jinbiao cushion is provided with Lyme disease recombinant antigen-colloidal gold conjugate. The detection test paper has the advantages of short detection time, high detection accuracy, high specificity and convenience in operation, and does not need the help of other equipment instruments.

The invention relates to a reagent card for detecting lily symptomless virus by adopting colloidal gold immunochromatographic assay and a preparation method. The reagent card comprises a reagent card slot, a lining board, a sample pad, a colloidal gold combining pad, a nitrocellulose membrane and suction filter paper, wherein the colloidal gold combining pad contains a golden probe, the lining board is fixed in the reagent card slot, the sample pad, the colloidal gold combining pad, the nitrocellulose membrane and the suction filter paper are arranged and connected to the upper surface of the lining board in sequence, a detection line and a contrast line are formed on the nitrocellulose membrane, and a rabbit anti-LSV polyclonal antibody and a sheep anti-rabbit IgG purification antibody respectively coat the detection line and the contrast line. The preparation method of the reagent card comprises the following main steps: coating an immunochromatographic membrane by the rabbit anti-LSV polyclonal antibody, and then preparing the colloidal gold combining pad. The reagent card has the advantages of rapidity in detection, high accuracy, strong specificity, simplicity and convenience in operation and no need of special equipment and instruments.

The invention discloses a rapid detection system for pesticide residues of fruits and vegetables and application of the rapid detection system, and belongs to the technical field of detection of pesticide residues. The rapid detection system disclosed by the invention comprises a plurality of slave computer detection modules and at least one host computer monitoring module, wherein the slave computer detection modules are used for detecting the concentration of the pesticide residues in a to-be-detected sample, and generating a detection signal; the detection signal is output through a printer after being subjected to conversion; meanwhile, detection information is transmitted to the host computer monitoring module; the host computer monitoring module is used for monitoring the detection states of the slave computer detection modules, collecting the detection result and automatically introducing the detection result into a database sub-module; meanwhile, query of the detection information can be achieved; and the database sub-module is used for collection, classified storage and analysis treatment of the detection information. According to the rapid detection system disclosed by the invention, rapid and multi-channel detection of the pesticide residues is achieved, the detection efficiency is improved; the detection cost is reduced; and the requirements of field detection of the pesticide residues can be fully met.

The invention discloses specific primers which are adopted in an LAMP detection method for species identification of Peru liquids or highly processed products thereof and comprise the upstream outer primer (F3), the downstream outer primer (B3), the upstream inner primer (FIP) and the downstream inner primer (BIP). The invention further provides a method for identifying the Peru squids and the highly processed products thereof through a loop-mediated isothermal amplification technique. The method comprises the following steps of 1 extracting of genome DNA in a fish sample to be detected; 2 designing of the Peru squid LAMP amplification primers; 3 setting of a real-time fluorescent LAMP system and an LAMP staining method system; 4 judging according to detection results of the real-time fluorescent LAMP system and the LAMP staining method system.

The invention relates to a detection method for sensitively and rapidly detecting staphylococcus aureus enterotoxin B, comprising the following steps: heating H1 and H2 with concentration of 5Mum at 95 DEG C for denaturation for 5min, heating an SEB aptamer at 85-95 DEG C for denaturation for 5-10min, then taking 6.7-33.3muL of SEB aptamer with concentration of 1muM, adding 100muL of SEB, mixing and then adding 80-100muL of hybrid buffer solution, and reacting for 30min at 37 DEG C; adding 6.7-33.3muL of 1muM H0, and reacting for 30min at 37 DEG C; adding 10-30muL of mixed reaction system of 100muL of H1 with concentration of 5muM and 100muL of H2 with concentration of 5muM; reacting for 45min at 37 DEG C constant temperature, and measuring the fluorescence value; compared with a conventional detection technology based on nucleic acid aptamer, the detection sensitivity is reduced by 2 orders of magnitudes.

The invention discloses a glycopeptide antibiotic drug-resistance gene detection kit. The glycopeptide antibiotic drug-resistance gene detection kit comprises probes 1 to 23, and corresponding sequences are shown as SEQ ID NO. 1 to SEQ ID NO. 23; the invention further discloses a method for detecting a glycopeptide antibiotic drug-resistance gene by utilizing the kit; when the glycopeptide antibiotic drug-resistance gene detection kit disclosed by the invention is used for detecting, the credibility of an experiment result needs to be evaluated at first; the detection result of the group is credible only when detection results of positive and negative quality control contrasts are positive (+) and negative (-). When the results displayed by the three probes of a detected object are consistent, whether a sample is infected by the glycopeptide antibiotic drug-resistance gene or not can be judged; when the results displayed by the three probes of the detected object are inconsistent, whether the sample to be detected is positive or negative cannot be judged, and the detection kit needs to be used for redetecting or existing other detection methods are used for further identification. The glycopeptide antibiotic drug-resistance gene detection kit is simple to operate, rapid and efficient and high in accuracy and the results are easy to read.

The invention relates to a method and device for measuring the length of a concealed columnar steel structure based on an ultrasonic guided wave sound field regulation and control technology, and belongs to the field of nondestructive testing and length measurement. The concealed columnar steel structure is a core-shell structure with a coating layer, such as a side slope anchor rod, a road guardrail stand column and the like. The device comprises a mobile device, a monitoring host and an ultrasonic guided wave transducer which are partially or completely connected in a wired or wireless mode.The method comprises the steps of standard library establishment, main frequency detection, modal detection, wave structure automatic recommendation, transducer working parameter and installation mode automatic recommendation and guided wave sound field regulation and control. According to the method, the guided wave sound field is regulated and controlled by analyzing and using the propagation characteristics of the ultrasonic guided waves and installing and arranging the transducers, so that the received echo signals only contain target modes, the multi-mode problem in traditional ultrasonic guided wave detection is solved, and the method has important engineering significance.

The invention discloses a portable potentiostat application platform based on vitamin B detection and aims to overcome defects of low measurement precision and large control errors of an existing potentiostat. The portable potentiostat application platform is characterized in that a power module is connected with a power input end of a microcontroller module; a D/A (digital-to-analog) conversion module converts digital quantity of the microcontroller module into analog quantity and transmits the analog quantity to an operational amplifier detection module; an A/D (analog-to-digital) conversion module converts sampling data of the operational amplifier detection module into digital quantity from the analog quantity and transmits the digital quantity to the microcontroller module; pull-in of a relay unit module is controlled through switching of high and low levels of a PC port in the microcontroller module, so that the connection state of a working electrode of the operational amplifier detection module is controlled; a key display module sends set parameters to the microcontroller module for processing and displays data received from the microcontroller module; an RS485 transmission module is connected with the microcontroller module and transmits detected signals to a GPRS (general packet radio service) wireless communication module. The portable potentiostat application platform based on vitamin B detection can detect trace vitamin B and can be combined with an electrochemical impedance test system for application.

The invention discloses a test strip for detecting citrobacter freundii and a preparation method thereof. The test strip comprises a sample pad, a combining pad, a nitrocellulose membrane, a water-absorbing pad and a PVC (Polyvinyl Chloride) bottom board. The test strip is characterized in that the sample pad, the combining pad, the nitrocellulose membrane and the water-absorbing pad are adhered in order and sequence on the PVC bottom board; the combining pad is coated with an anti-citrobacter-freundii egg-yolk antibody-colloidal gold marker; and the nitrocellulose membrane is coated with a detection line formed by citrobacter-freundii ultrasonic breaking liquid and a quality control line formed by rabbit-anti-chicken egg-yolk antibodies. The test strip disclosed by the invention has the advantages of high sensitivity, strong specificity, simplicity in operation, fast detection and high accuracy.

The invention provides a sleeve sealed inside diameter test gauge. The test gauge mainly comprises a depth screw, a locking nut, a gauge device, an indicating gauge dial plate, a connecting rod, a contact, a reference backup plate and a wear-resisting plate, wherein the reference backup plate is arranged above the wear-resisting plate; the wear-resisting plate is connected with the depth screw by the locking nut; the gauge device is connected below the depth screw; the depth screw is connected with the indicating gauge dial plate and the connecting rod by the gauge device respectively; and the contact is arranged at the end of the connecting rod. The sleeve sealed inside diameter test gauge has high adjustable property, meets the sealed diameter measurement of different apertures and the requirements of on-site detection, and is convenient to detect and easy to operate.

The invention discloses a toxoplasma gondii dense granular protein GRA1. A nucleotide sequence for encoding the protein is shown as SEQ ID NO:1. The invention further discloses an application of the dense granular protein GRA1 to the preparation of an enzyme-linked immunoassay detecting kit for treating toxoplasmosis and an enzyme-linked immunoassay method for detecting a sheep toxoplasma gondii IgG antibody. The method has the advantages such as high sensitivity, strong specificity, good accuracy as well as high speed, simplicity and convenience when being used for detecting the toxoplasmosis.

The invention discloses toxoplasma detecting parallel probes. The toxoplasma detecting parallel probes comprise a probe 1, a probe 2 and a probe 3, with respective sequences as follows: SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. The invention further discloses a gene chip comprising the probes as well as a kit comprising the gene chip, and further discloses a method for detecting toxoplasma by using the kit. The method comprises the following steps: detecting by using the toxoplasma detecting kit provided by the invention, wherein when detection results displayed by the three probes of a detected object are consistent, whether a sample is infected by the toxoplasma can be judged, and when the detection results displayed by the three probes of the detected object are inconsistent, whether the sample to be detected is positive or negative cannot be judged; and redetecting by using the detecting kit or further verifying by using an existing other detection method. Through toxoplasma detecting parallel probes, the gene chip, the kit and the detection method, operation is simple, results are easy to read, needs of hospital detection, prenatal detection and other on-site detections are met, and the results are accurate and reliable.