Enzyme-linked immunoassay method for detecting sheep toxoplasma gondii igg antibody

An enzyme-linked immunosorbent assay, Toxoplasma gondii technology, applied in immunoassays, chemical instruments and methods, botanical equipment and methods, etc., can solve the problems of inappropriate large-scale inspection, low detection rate, time-consuming and laborious, etc., and achieve high sensitivity. , the effect of good specificity and easy automation
CN109369796AInactive Publication Date: 2019-02-22HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2019-02-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a toxoplasma gondii dense granular protein GRA1. A nucleotide sequence for encoding the protein is shown as SEQ ID NO:1. The invention further discloses an application of the dense granular protein GRA1 to the preparation of an enzyme-linked immunoassay detecting kit for treating toxoplasmosis and an enzyme-linked immunoassay method for detecting a sheep toxoplasma gondii IgG antibody. The method has the advantages such as high sensitivity, strong specificity, good accuracy as well as high speed, simplicity and convenience when being used for detecting the toxoplasmosis.
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Description

technical field

[0001] The invention relates to an enzyme-linked immunosorbent method (ELISA) for detecting goat toxoplasma IgG antibody, and also relates to a toxoplasma gondii dense granule protein GRA1 which can specifically react with goat toxoplasma gondii IgG antibody. technical background

[0002] Toxoplasma gondii is a zoonotic protozoan widely distributed in the world, which can cause diseases in humans and various animals. It is estimated that about 1 / 3 of the world's people are infected with Toxoplasma gondii. Among animals, pigs have the highest infection rate, generally above 20%, and some farms even reach 100%. Sheep are the most sensitive animals to Toxoplasma gondii except mice. After being infected with Toxoplasma gondii, sheep are prone to miscarriage, stillbirth, weak fetus, etc. In some countries, up to 20% of sheep abortions are caused by Toxoplasma gondii infection. industry has caused great losses. In China, the average infection rate of flocks is ar...

Claims

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