58 results about "Babesia" patented technology

The invention discloses a primer, a probe and a kit for fluorescence quantitative PCR (Polymerase Chain Reaction) detecting and typing of babesia. According to the invention, the primer sequence is as shown in SEQID (SEQuence IDentifier) NO.1, 2 and 3 and the probe is as shown in SEQIDNO.4 and 5. A typing method of the babesia comprises the following steps of: carrying out PCR amplification on the to-be-detected bacterium by using the primer and the probe to obtain Erich bacterium positive samples of 290bp (base pair) bands, wherein the fluorescence of the samples is enhanced at the wavelength of 640nm in fluorescence detection; and typing the babesia according to the melting temperature. The primer, the probe and the kit disclosed by the invention have obvious technical advantages, have very high specificity and sensitivity and can be used for conveniently detecting the babesia of important species.

The invention relates to a membrane for detecting cattle and sheep Giardia in Haemaphysalis qinghaiensis and distinguishing the type. The membrane for detecting Theileria luwenshuni, Theileria uilenbergi and Giardia in cattle and sheep is prepared by fixing specific oligonucleotide of the Theileria luwenshuni and the Theileria uilenbergi or the specific oligonucleotide of Babesia and the universal specific oligonucleotide of Theileria on a Biodyne C membrane. The method for detecting comprises the steps: using DNA extracted from Haemaphysalis to be detected as a template; using a specific primer to perform PCR amplification; hybridizing the product with the oligonucleotide of the Theileria and Babesia for detecting; putting the hybridized membrane in an exposed template holder after enhanced chemiluminescence incubation; putting an X-ray film on the membrane for treatment of exposure, developing and the like; and determining whether the detected animal has the corresponding disease according to an intensity signal produced on the film.

The invention discloses a babesia mocroti Bm7 recombinant antigenic protein, which comprises protein with an amino acid sequence shown in a SEQ ID NO.2, or a protein with the same functions formed by replacing and deleting or inserting the protein. In addition, the invention also discloses a preparation method of the babesia mocroti Bm7 recombinant antigenic protein, which comprises the steps of babesia mocroti Bm7 gene sequence amplification, babesia mocroti Bm7 recombinant plasmid construction and identification, and inducible expression and purifying of the recombinant protein. In addition, the invention also discloses an application of babesia mocroti Bm7 recombinant antigenic protein in preparation of a reagent or a kit for detecting babesia mocroti disease patients (animal or human) serum antibody. Through verification, the babesia mocroti Bm7 recombinant antigenic protein used for babesia mocroti diagnosis has high sensitivity and singularity, is latent diagnosis candidate target, and can be taken as a target antigen for babesia mocroti disease diagnosis.

The invention discloses a molecular beacon mediated isothermal amplification detection method combining with a nucleic acid chromatography detection technology and application thereof. The method canbe used for detecting pathogen genomes in various biofluid media, such as various unknown contaminant bacteria in blood platelet or tiny babesia propagated through blood transfusion. A molecular beacon mediated isothermal duplication product is detected by a nucleic acid chromatography method; the possibility of the isothermal duplication technology in the direction towards instant inspection (Point-Of-Care Technology, POCT) is enhanced; a kit can be further prepared; wide application can be realized in the fields of emergency / war wound emergency rescue and treatment, inspection and quarantine, sudden infectious disease inspection and monitoring, Point-of-Care Testing and the like; the application prospects are wide.

The invention provides a bovine babesiosisbigemina nested PCR specific primer as well as a detection kit and a nested PCR detection method. The method is simple in template DNA preparation steps and low in cost; the detection of the method is extremely convenient and quick; specificity and sensitivity of the detection method can be improved; trace genome DNA extracted by single tick and even partof tissue and organs of the tick can be used as a template for identifying; four specific primers are designed for the specific area of bovine babesiosisbigemina taking RAP-1 as target gene; the defect of cross reaction between bovine babesiosisbigemina and bovine babesia when diagnosing the babesia in the tick body through the existing PCR detection technology is solved. The method is higher in reaction specificity and higher in sensitivity, is specially applied to accurate detection of the bovine babesiosisbigemina, and has broad application prospects.

The invention belongs to the field of biomedical researches and clinical application, and particularly relates to a specific method for quickly and serologically detecting babesiasis with high throughput based on micro-fluidic chips and application thereof. The method for detecting the serum antibody of a babesia antigen in the serum of a babesiasis patient is provided based on the micro-fluidic chips. Comparison and verification with traditional serology including an enzyme linked immunosorbent assay prove that the diagnosis result of the method for serologically detecting babesiasis based on micro-fluidic chips is consistent with that of traditional serology. Furthermore, the method has the characteristics of low serum sample usage, multi-antigen detection, low reagent and energy consumption, low detection cost, high automatic degree and prevention of pollution among samples. The method can further provide a magnificent reference base for serum immunological diagnosis of patients with babesiasis.

The invention discloses application of a reagent combination for detecting pathogens in preparation of a kit for detecting the quality of a blood product. The pathogens are selected from a pathogen group consisting of HAV, HBV, HCV, HEV, TP, HIV1, HIV2, HTLV1, HTLV2, HBOV, TTV, HCMV, B19, DENV, ZIKV, WNV, babesia, trypanosoma krusei and plasmodium, and belongs to the field of medical examination.The invention further discloses a corresponding kit and a method for detecting the quality of the blood product. By means of the kit, the lowest detection limit of pathogens is 1.0 * 10 < 3 > copies / mL, the kit has good specificity and repeatability, and an important technology is provided for screening and evaluating blood transfusion transmitted pathogens.

The invention discloses a kit for detecting and identifying babesia motasi through the combination of isothermal amplification of cross primers and immunochromatography and a use method of the kit. The babesia motasi detection kit comprises five primers for isothermal amplification, namely a cross primer with the sequence of SEQ ID No.3, two replacement primers with the sequences of SEQ ID No.1 and SEQ ID No.2, a probe primer with the sequence of SEQ ID No.4, and a biotin labelled probe primer with the sequence of SEQ ID No.5. The kit further comprises an immunochromatographic test strip. Thekit has the advantages of convenience in operation, speediness, high sensitivity, high specificity and wide scope of application, and the detection result can be read by naked eyes.

The invention discloses a babesia orientilas thrombin gene 1 which has the nucleotide sequence shown in SEQ ID NO:1. The invention further discloses recombinant protein obtained by coding the babesia orientilas thrombin gene 1, wherein the recombinant protein has the amino acid sequence shown in SEQ ID NO:2. The recombinant protein can effectively detect the existence of antigens of a water buffalo infected with the babesia orientilas, has good reactogenicity, belongs to a candidate factor for developing a serological diagnosis method for detecting the babesia orientilas disease, and is beneficial for controlling the prevalence and spread of the babesiosis disease of the water buffalo.

The invention discloses a method and a special kit thereof for detecting babesia. The invention discloses a group of DNA molecules which include DNA molecules as shown in (1)-(3): (1) a DNA molecule as shown in SEQ ID No.2; (2) a DNA molecule as shown in SEQ ID No.3; and (3) a DNA molecule as shown in SEQ ID No.4. The method disclosed by the invention, compared with morphological and immunofluorescent methods adopted in clinical field in the past, can greatly improve the sensitivity of detecting babesia, and the method has the characteristics of being rapid and convenient; and the method and the special kit thereof are suitable for basic hospitals and suitable for monitoring babesia propagation media and animal hosts.

The invention discloses a primer, a probe and a kit for fluorescence quantitative PCR (Polymerase Chain Reaction) detecting and typing of babesia. According to the invention, the primer sequence is as shown in SEQID (SEQuence IDentifier) NO.1, 2 and 3 and the probe is as shown in SEQIDNO.4 and 5. A typing method of the babesia comprises the following steps of: carrying out PCR amplification on the to-be-detected bacterium by using the primer and the probe to obtain Erich bacterium positive samples of 290bp (base pair) bands, wherein the fluorescence of the samples is enhanced at the wavelength of 640nm in fluorescence detection; and typing the babesia according to the melting temperature. The primer, the probe and the kit disclosed by the invention have obvious technical advantages, have very high specificity and sensitivity and can be used for conveniently detecting the babesia of important species.

The invention is directed to methods of using novel Babesia antigen peptides in detecting Babesia spp. in a sample. The methods may be adapted to assays suitable for high blood volume screening, use for clinical diagnosis of patients with babesiosis, or assaying blood contaminated with Babesia spp., such as B. microti.

The present invention relates to methods for stable transfection of Babesia parasites, and for vaccines conferring immunity against parasitic arthropods.

The invention discloses a diagnostic kit and a detection method for detecting sheep Xinjiang babesia U sp. through rapid nested PCR. The sheep Xinjiang babesia red blood cell invasion related protein (rap-1) is adopted as the target gene, and 4 bidirectional nested specific primers are designed and synthesized according to the rap-1 gene sequence to form specific primers XJ-full F(5'-CGGTAGTGTGTTGATATCCTAGAG-3'), XJ-full R(5'-CACAAGCGTTACATCGTAGAGCCG-3'), XJ-internal F(5'-AATGACCGCCCGCGAAATCAGCCACGA C-3') and XJ-internal R(5'-GTGGGTGGCGAAGGACAATTTGTTG-3'); and a nested PCR detection method is established for the sheep Xinjiang babesia of a field blood sample.

The invention discloses a field mouse babesia RPA molecular detection method. The field mouse babesia RPA molecular detection method comprises the following steps: designing a pair of specific primersand probes according to field mouse babesia mitochondria COX I gene sequence, wherein the nucleotide sequences of the primer pair is shown as SEQ ID NO: 2 to SEQ ID NO: 4 respectively; then extracting total DNA inside a dog blood sample to carry out recombinase combined enzymatic amplification reaction; and finally adopting a lateral flow analysis test paper strip to carry out detection on an amplification product. The method is high in sensitivity and high in specification, and field mouse babesia can be identified rapidly.

The invention relates to the technical field of molecular biomedicine, in particular to a PCR (Polymerase Chain Reaction) detection kit for cat and / or dog pathogens, a detection method and application. The kit is used for detecting canine distemper virus, canine influenza A virus, canine parainfluenza virus, canine parvovirus, canine coronavirus, canine rotavirus, canine babesia, canine ascaris, canine Ehrlichia, canine brucella, rabies virus, borrelia burgdorferi, reference gene ACTB, feline herpes virus, feline calicivirus and feline parvovirus. The kit comprises primers and probes of feline coronavirus, feline immunodeficiency virus, feline leukemia virus, feline mycoplasma, feline mycoplasma, feline chlamydia, giardia, toxoplasma, bartonella and reference gene GAPDH, collected DNA and RNA are added into the kit, a real-time fluorescence PCR instrument is adopted for PCR reaction, FAM, HEX, ROX and CY5 fluorescence signals are collected in each cycle, analysis of related pathogens is carried out, and the kit can be used for detecting the feline and the canine. Compared with a traditional detection method, the method has the advantages of higher specificity and higher sensitivity.

Methods for the rapid detection of the presence or absence of Babesia in a biological or non-biological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers and probes targeting Babesia and kits are provided that are designed for the detection of Babesia, including, but not limited to, the Babesia species of B. microti, B. divergens, B. duncani, and B. venatorum. Also described are kits, reaction mixtures, and oligonucleotides (e.g., primer and probe) for the amplification and detection of Babesia.

The invention discloses a method for detecting ovine babesiasis indeterminate species, Babesia motasi LZ subspecies and Babesia motasi NB subspecies as well as a detection kit thereof. The detection method takes ovine babesiasis thrombospondin-related anonymous protein TRAP gene as a detection identifier. The kit comprises six primers shown in SEQ ID No.1, SEQ ID No.2, SEQ ID No.3, SEQ ID No.4, SEQ ID No.5, and SEQ ID No.6, The method takes TRAP as the detection identifier, the difference between species and subspecies is large, the detection sensitivity is better than that of the current technologies. The kit can simultaneously amplify several target DNA molecules in a PCR reaction, and realizes a purpose of simultaneously discriminating and detecting a plurality of microbes in a same sample. Compared with the other molecule detection technologies, the method has the advantages of simpleness, rapidity, high efficiency and saved cost, is suitable for rapid detection of cause of disease, and can be a hotspot technology for scientific research and clinical diagnosis.

The present invention relates to methods for stable transfection of Babesia parasites, and for vaccines conferring immunity against parasitic arthropods.

Methods and compositions for preventing and / or treating diseases and conditions caused by certain apicomplexan infections are provided. The methods involve administering / providing to a subject one or more of tartrolon D and E, including isomers thereof. Exemplary apicomplexan infections that are prevented / treated by the compounds of the invention include those caused by Cryptosporidium, Babesia, Cyclospora, Isospora, Plasmodium, Sarcocystis, Besnoitia, Hammondia, Neospora, Theileria and Toxoplasma.

The invention discloses a monoclonal antibody resistant to vole babesia. The monoclonal antibody is secreted by a hybridoma cell strain HA5-E4 having a collection number of CCTCC NO: 201835. Aiming atlactic dehydrogenase LDH in a glucide metabolic pathway of the vole babesia, the protein has immunogenicity, and a monoclonal antibody aiming at rBmLDH is prepared; Western blot detection proves thatthe monoclonal antibody is capable of specifically recognizing the vole babesia but cannot recognize the LDH of the host. Therefore, the monoclonal antibody can be applied to detection of vole babesia in infection cells through an immumofluorescence method.

An antigen composition with plurality of antigens from B. microti, and methods of predicting a likelihood of an individual having B. microti infection using the antigen composition as biomarkers of infections of B. microti to human are provided. In particularly preferred aspects, the antigens are immunodominant and have quantified and known relative reactivities with respect to sera of a population infected with the pathogen with an average discriminatory p value of ≤0.05.

The present invention discloses a cross-primer constant temperature amplification method and a kit used for identifying and detecting babesia unidentified species. The constant temperature amplification kit for identifying and detecting the babesia unidentified species comprises a cross primer BXJ-2a1s having a sequence of SEQ ID No.3, a replacement primer BXJ-5a having a sequence of SEQ ID No.1 and a replacement primer BXJ-4s having a sequence of SEQ ID No.2, respectively, a probe BXJ-2a having a sequence of SEQ ID No.4 and a probe BXJ-3a having a sequence of SEQ ID No.5, respectively, conventional reagents for amplification, and lateral flow chromatography test strips required for determination of results. The detection method can specifically detect the babesia unidentified species, isfast, simple in operation, strong in specificity, high in sensitivity, free of complicated instruments and equipment, and intuitive and visible in results, and very suitable for rapid detection at grass-root sites and has relatively large application prospects.

The invention discloses a babesia mocroti 2D41 antigen protein and application thereof. The protein has an amino acid sequence as shown by SEQ ID NO:8, and has an encoded gene sequence as shown by SEQID NO:7. The babesia mocroti 2D41 antigen protein has a continuous high-immunity reaction with mouse serum of 14dpi-270dpi in a specific IgG antibody reaction and has an effect of remarkably reducingparasitemia in immunity protection testing, and the average parasitemia value of the protein is reduced by about 35% when being compared with those of an adjuvant group and a natural immunity group.The 2D41 antigen protein can be used for detecting or diagnosing babesia mocroti diseases, and for preparing treatment and / or prevention medicines for reducing parasitemia.

The present invention provides an isolated gene, the sequence of which is shown in SEQ ID NO:3. The present invention also provides a Babesia microti Bm1524 antigenic protein, which is encoded by the above isolated gene, and its amino acid sequence is shown in SEQ ID NO:4. The present invention also provides an antibody that specifically binds to the above-mentioned Babesia microti Bm1524 antigen protein. The present invention has further established a highly specific and sensitive diagnostic method for Babesia microti through the expression and purification of Babesia microti Bm1524 antigen, which can be applied to different aspects such as scientific research and prevention of Babesia microti; the present invention The vaccine has the effect of preventing Babesia vole infection. The kit and the colloidal gold immunochromatographic test strip of the invention can also be used to detect Babesia microti.

The invention discloses a fluorescent quantitation PCR (photo-conductive relay) primer combination and method for rapid detection of dog babesia and a kit with the fluorescent quantitation PCR primer combination and belong to the technical field of biological detection. The fluorescent quantitation PCR primer combination is designed for a dog babesia 18S RNA sequence, and a primer is high in specificity, free of interference of other nonobjective genes and capable of determining the transcriptional level of the dog babesia and quantifying the number of the dog babesia rapidly efficiently and accurately. The dog babesia is quantitively detected with the fluorescent quantitation PCR method which is simple, accurate and free of pipe contamination and gel electrophoresis, quantitative results can be acquired by directly detecting change of fluorescence signals in the PCR amplification process through a photoelectric conduction system, result repetition is good, detection sensitivity is high, and detection on high-throughput samples can be achieved.

The invention discloses Babesia mocroti antigen proteins 2D33 and 2D36 and application of the Babesia mocroti antigen proteins. The amino acid sequences of the Babesia mocroti antigen proteins are respectively represented by SEQ ID NO:4 or SEQ ID NO:6, and an encoding gene sequence of Babesia mocroti antigen proteins is represented by SEQ ID NO:3 or SEQ ID NO:5. An immunoreactions spectrum of Babesia mocroti is acquired, the immunoreactions of recombinant proteins Bm2D33 and Bm2D36 with mouse serum after an acute stage are obviously decreased in the protein chip analysis of specific IgG antibody, and the recombinant proteins Bm2D33 and Bm2D36 can be used for detecting disease transformation progress of Babesia mocroti from the acute stage to a chronic stage. The recombinant proteins Bm2D33and Bm2D36 present very high specificity, namely 100% in the cross reaction tests of the serum of patients with falciparum malaria.

The invention discloses a globoid protein gene 4 of babesia orientalis. The gene has a nucleotide sequence as shown in SEQ ID NO: 1. The invention further discloses a protein for encoding the globoidprotein gene 4 of the babesia orientalis. The protein has an amino acid sequence as shown in SEQ ID NO: 2. The protein can effectively detect the existence of a babesia orientalis antigen in a buffalobody, has high immunogenicity, can detect the existence of the babesia orientalis by applying an indirect immunofluorescence method, and can be used as a candidate molecule for diagnosing an antigen.