40 results about "Ascaris" patented technology

The invention discloses preparation and application of a giant panda Ascaris schroederi Mcintosh antigen, which belong to the giant panda Ascaris schroederi Mcintosh prevention, treatment and detection field. The method comprises the following steps: a Ascaris schroederi Mcintosh antigen gene primer is designed; total RNA of ascarids undergoes reverse transcription by the RT-PCR method to synthesize cDNA, and then the cDNA is taken as a template for PCR amplification of a target product; the purified target product is connected with a pMD18-T vector and then converted into DH5 alpha competent bacteria; positive recombinant clone is selected through flat screening and culture of the bacteria, and the antigen genes have a Bs-Ag1gene, a Bs-Ag2 gene and a Bs-Ag3 gene after culture and sequencing; and then recombinant plasmids which are accurately sequenced are converted into Escherichia coli BL21 competent cells for mass expression of proteins after construction of the recombinant plasmids, induction expression and purification of the recombinant proteins. After detection and immunologic tests of the product and ELISA detection and analysis of a test animal antibody IgG, the Bs-Ag1gene, the Bs-Ag2 gene and the Bs-Ag3 gene of the giant panda Ascaris schroederi Mcintosh antigen can be taken as candidate genes for preparing genetic engineering vaccines through the sascarids; and the recombinant proteins Bs-Ag1, Bs-Ag2 and Bs-Ag3 have good reactionogenicity and can be used for detecting infection of giant panda Ascaris by the ELISA method.

The invention provides an amino acid-containing cassava lees organic fertilizer. The organic fertilizer contains the following raw materials in parts by mass: 800-900 parts of cassava lees, 175-250 parts of plant ash, 50-100 parts of plant protein and 0.2-0.4 part of composite strain. The organic fertilizer provided by the invention is prepared through fermentation by adopting cassava lees as main raw material, the organic content is over 60%, the content of total nutrients is over 8.5%, the content of total amino acids is over 1%, the content of nitrogen phosphorus and potassium is over 2.3, and the water content is about 27%, the organic fertilizer is slightly alkaline, the death rate of ascaris eggs exceeds 99%, and the requirements of NY525-2012 are met; and moreover, the cost is low, the fermentation time is short, the whole fermentation process is controllable, the problem of incomplete fermentation or excessive composting is overcome, the environmental pollution caused by solid wastes is effectively reduced in the alcohol production process, and the production requirements of environmental protection are met.

The plum beverage disclosed by the invention is prepared from wild jujube peel, smoked plum, haw, white sugar, citric acid, benzoic acid as raw material and through the steps of water extraction, filtering liquid combination, injecting into sugar melting pot, heating to boiling, charging formula amount of white sugar and stirring, melting the sugar and concentrating.

The invention discloses an electrochemical sludge deep dewatering device and a sludge deep dewatering method. The equipment includes mainframe support, feeder, sludge conveyor belt, conveyor belt drive mechanism, one or more series-connected electrochemical sludge deep dewatering mechanisms, mud cake separation mechanism, sludge conveyor belt descaling and cleaning system; The deep mud dewatering mechanism is composed of lower plate, lower plate electrode, upper plate, upper plate electrode, electrode insulation plate, upper plate sealing cover, sealing spring, spring pull rod, pressurized hydraulic oil cylinder, and beam. The present invention utilizes the electrochemical phenomena and mechanical pressure after the sludge is added to the DC electric field for dehydration; it can reduce the moisture content of the activated excess sludge from 85% to below 40% at one time without adding any conditioning measures , to reduce the volume of the remaining sludge by more than 2/3, and through electrochemical action, it can destroy the microbial cell membrane in the sludge, kill roundworm eggs and pathogenic microorganisms in the sludge, and remove some heavy metals.

The invention relates to a traditional Chinese medicine composition for treating ascariasis of fur animals, which is prepared from dark plum fruit, rhizoma coptidis, aconite, pericarpium zanthoxyli, asarum sieboldi, rhizoma zingiberis, ramulus cinnamomi, radix sophorae falvescentis, cortex meliae and areca catechu. The traditional Chinese medicine composition can alternatively be a decoction, and is prepared according to the following steps: (1) measuring all traditional Chinese medicines according to a formula and putting into an extraction pot; (2) cooking the traditional Chinese medicines in the extraction pot for 2-12 hours by adding water 5-15 times of the total weight of the traditional Chinese medicines; (3) filtering to remove medicine residues and keeping the filtrate for later use; (4) cooking the medicine residues in the extraction pot for 2-12 hours by adding water 5-10 times of the weight of the residues; (5) filtering to remove medicine residues and combining the obtained liquid with the first extraction liquid; and (6) vacuum-concentrating the mixture at 60 DEG C to 10-50 percent of the original volume, filtering to remove residues, filling according to 200 ml per bottle. The traditional Chinese medicine composition provided by the invention has the effect of clearing away heat, secreting bile, relieving ascaris colic, stopping vomiting, expelling ascaris and stopping pain, and has over 98% cure rate of ascariasis of fur animals.

The invention provides biological bacterial fertilizer prepared from poultry and livestock waste. The biological bacterial fertilizer meets the following indexes that the number of effective living bacteria is greater than or equal to 42 million/g; the content of N+P2O5+K2O is greater than or equal to 8%, and the content of organic matter is greater than or equal to 45%; the content of amino acids is greater than or equal to 6%; the content of humic acid is greater than or equal to 5%; the germination index of seeds is greater than or equal to 95%; the content of soluble carbon is smaller than or equal to 0.56; the number of coliform bacteria is smaller than or equal to 60/g; the mortality of ascaris eggs is greater than or equal to 97%; the fertilizer has no pungent odor. The invention further discloses a preparation method of the biological bacterial fertilizer prepared from the poultry and livestock waste. The method comprises the step of preparing carrier materials; the prepared biological bacterial fertilizer is long in service life and especially beneficial to crop growth and seeding, and has beneficial strains, various enzymes and trace elements such as various vitamins, carbohydrate, cellulose and nucleic acid; the microbial activity of soil can be enhanced, the number of beneficial bacteria in root-layer soil is increased, and the activity of the beneficial bacteria in the root-layer soil is improved.

Discloses is a traditional Chinese medicine used for treating colic caused by ascaris, belonging to traditional Chinese medicines for treating colic caused by ascaris. The compositions of the medicine are 30 grams of capillary wormwood herb, 30 grams of betel nut, 12 grams of dark plum, 12 grams of hawthorn fruit, 12 grams of zanthoxylum piperitum, 12 grams of prepared aconite root, 3 grams of asarum, 3 grams of coptidis rhizoma, 3 grams of cassia twig, 3 grams of evodis rutaecarpa, 6 grams of of phellodendron bark, 6 grams tangerine peel and 9 grams of dry ginger. The worms dispelling prescription takes 30 grams of chinaberry bark and 20 grams of betel nut as auxiliary prescription, and when pulse sinks, extremities are cold, and physical quality is poor, 10 grams of ginseng and 10 grams of angelica sinensis are added, and when patients are infected with constipation, 12 grams of rheum officinale and 12 grams of matrii sulfas exsiccatus are added. The drug dispensing materials of the traditional Chinese medicine are all natural Chinese herbs, the traditional Chinese medicine is then made with a traditional processing method, and the materials of the medicine are easy to get, the prescription and preparation methods are simple, and the costs of preparing drugs are low. The advantages of the medicine are that the Chinese herb has simple compatibility, the drugs used in the drug prescriptions are totally natural Chinese herbs, fetching and using of the medicine are easy, the process for preparing the medicine is simple, the curing effect of the medicine after the medicine being taken is excellent, and the cost of the medicine is low.

The invention discloses a traditional Chinese medicine composition for treating ascariasis. The traditional Chinese medicine composition is prepared from the following raw materials in a weight ratio: 8-15 g of coptis chinensis, 10-20 g of dark plum fruit, 5-12 g of cortex phellodendri, 12-20 g of fructus quisqualis, 5-12 g of wild ginger, 15-25 g of glabrous greenbrier rhizome, 10-20 g of radix paeoniae rubra, 8-15 g of codonopsis pilosula and 4-10 g of Chinese honeylocust fruit. According to the traditional Chinese medicine composition for treating ascariasis disclosed by the invention, glabrous greenbrier rhizome is used as a main drug, other adjuvant drugs are added, and the drugs interact with each other and have synergistic effects so as to achieve excellent efficacies of regulating qi and alleviating pain, relieving ascaris colic and expelling parasites, clearing heat and benefiting gallbladder as well as removing stagnation; the traditional Chinese medicine composition has good treatment effects on ascariasis, and is safe and free of side effects, simple in preparation method and convenient to take.

The invention relates to a fermentation technology of biological organic bacterial manure, and belongs to the technical field of biological organic bacterial manure manufacturing. The technology includes the steps that the raw materials of chicken manure, tobacco waste and cereal, cotton and oil processing plant waste are piled at the normal temperature to be naturally dehydrated; then, a compound fermentation strain is added, and after two times of fermentation and ageing treatment, the biological organic bacterial manure is obtained. By means of the technology, escherichia coli, ascaris eggs and some infectious germs in the chicken manure can be killed in the decomposing process, worm eggs or harmful pathogens or weed seeds in the mixed materials can be thoroughly killed through ageing treatment, and thus the biological bacterial manure is deeply decomposed and has high fertilizer efficiency. The technology is environmentally friendly in production process, short in decomposing period and small in nutrient loss; experiments on the crops of rice, lactuca sativa L, cotton and the like show that the yield is greatly increased, and pest and disease damage is effectively controlled. The problems that in a traditional fermentation method, maturity is low, pathogens and weed seeds in raw materials can not be completely killed, the fermentation process pollutes the environment and fertilizer efficiency is low are solved.

The invention relates to a Chinese medicinal pill which is prepared from pharbitis seed, areca-nut each 100g, omphalia, banksia rose each 24g, oriental wormwood 50g, chinaberry bark, Chinese honey locust each 24g through grilling into dense extract and powder.

The invention relates to the field of health care products, and discloses applications of camellia oleosa seed oil. The application refers to the preparation of medicines for preventing and/or treating chronic pharyngitis, hypertension, cardiovascular diseases, abdominal pain caused by eruptive diseases, and ascaris intestinal obstruction. A preparation process of the camellia oleosa seed oil comprises the following steps: (1) carrying out first-time squeezing on camellia oleosa seeds at 35-45 DEG C, so that crude camellia oleosa seed oil is obtained; and (2) mixing the crude camellia oleosa seed oil, lucid ganoderma, rhizoma curcumae longae and chitosan, and carrying out second-time squeezing at 25-70 DEG C. Compared with the camellia oleosa seed oil prepared by adopting the traditional method, the camellia oleosa seed oil prepared by adopting the method provided by the invention has the obvious effects for preventing and/or treating chronic pharyngitis, hypertension, cardiovascular diseases, abdominal pain caused by eruptive diseases, and ascaris intestinal obstruction.

The invention discloses a drug for treating ascaris intestinal obstruction. The drug is prepared from, by weight, 5-10 parts of costus roots, 5-10 parts of cortex magnoliae officinalis, 1-5 pars of immature bitter oranges, 3-6 pars of rhubarb, 3-6 parts of fructus mume, 1-5 parts of semen arecae, 5-10 parts of fructus quisqualis, 1-5 parts of natrii sulfas, 1-5 parts of ginger, 5-10 parts of herba artemisiae scopariae, 5-10 parts of senna leaves, 1-5 parts of pericarpium citri reticulatae, 3-6 parts of dandelions, 3-6 parts of China ixeris, 5-10 parts of angelica sinensis, 1-5 parts of radix achyranthis bidentatae, 1-5 parts of honeysuckle flowers, 5-10 parts of fructus forsythiae, 1-5 parts of radix bupleuri, 3-6 parts of cinnamon, 1-5 parts of inula flowers and 1-5 parts of prepared common monkshood daughter roots. According to the drug for treating the ascaris intestinal obstruction, the compatibility is conducted on all drugs according to differentiation, and the effects of dispersing cold, warming innards, stopping vomit, moistening dryness, lubricating the intestines, removing toxicity, relieving pain and the like are achieved.

A traditional Chinese medicine for curing ascaris intestinal obstruction belongs to a traditional Chinese medicine for curing intestinal obstruction. The components include: 30g of smoked plum, 30g of rhubarb, 20g of zingiber and 100g of honey. The substances in the prescription are made from natural traditional Chinese medicine plus traditional preparing methods. The materials are easy to collect, the prescription and the preparation methods are simple, and the cost of medicine preparation is low. The compatibility of the traditional Chinese medicine is simple; the medicine used in the prescription is fully natural herbal medicine, which is easy to collect and use; the preparation method is simple; the effects for taking and curing are good; the cost of the medicine is low, and the medicine is in particular suitable for the people living in remote villages far from towns; the cost is low for curing the people suffering from ascaris intestinal obstruction disease, which solves the problem that the local area lacks medical conditions to cure the disease because of low household income and poor living condition.

The invention discloses a traditional Chinese medicine preparation for treating phlegm-dampness constitution type polyp, which relates to a traditional Chinese medicine preparation, and is used for treating patients with phlegm-dampness constitution type who are blocked by veins and will form gallbladder polyp in biliary tract after phlegm forms knot. The preparation includes: 60-100 parts of black plum, 50-60 parts of as arum, 60-80 parts of dried ginger, 30-50 parts of cortex phellodendri, 60-90 parts of fresh ginger and 700-800 parts of a gel composite liquid. The gel composite liquid is prepared from 28% of Chinese date juice, 42% of egg white, 21% of fine powder, 3% of K-carrageenan mixture, and 6% potassium chloride. The Chinese medicinal preparation has the effects of regulating qi,promoting blood circulation, clearing away heat and toxic materials, eliminating phlegm and dispersing stagnation, promoting gallbladder contraction and cholecystectomy, facilitating bile drainage, reducing or preventing biliary tract infection and ascaris eggs remaining in biliary tract to form gallbladder polyps, and reducing the occurrence of gallbladder polyps. The invention also provides a preparation method of a traditional Chinese medicine preparation for treating phlegm-dampness constitution type polyp.

The invention discloses a processing method of a bio-organic fertilizer. The method comprises the sequentially performed steps of mixing, fermentation, crushing, screening and drying. The mixing stepis performed before the fermentation; the raw materials are mixed to obtain mixed materials; the mixed materials comprise 10 to 20 percent of compost manure, 3 to 5 percent of additives and the balance of stalks and animal waste; the additives comprise 100 weight parts of fermentation strains, 1 to 3 weight parts of ferrous sulfate and 2 to 6 weight parts of humic acid. The organic fertilizer product meets the standard NY525-2012. The main index requirements are shown as follows: the organic matter content (metered by dry bases) is higher than or equal to 60 percent; the total nutrient (N+P2O5+K2O) content (metered by dry bases) is higher than or equal to 8 percent; the moisture (free water) content is lower than or equal to 30 percent; the PH is 5.5 to 8.0. The heavy metal content, the ascaris egg death rate and the colon bacillus value index in the organic fertilizer conform to the requirement of GB-8172.

This invention relates, e.g., to a method for suppressing inflammation (e.g. allergic inflammation, for example asthma caused by ragweed), a Th1-mediated condition and/or a Th2-mediated condition and/or a condition in which administration of an antioxidant would be beneficial, in a subject in need thereof, comprising administering to the subject an anitinflammatory-effective amount of Ascaris-derived heme-binding protein (HBP) or an active fragment or variant thereof (e.g. domain 2 of the polypeptide or an active variant thereof). Also described are pharmaceutical compositions for suppressing one of the above conditions, adjuvant compositions and immunogenic compositions comprising Ascaris-derived heme-binding protein (HBP), or an active fragment or variant thereof, and an antigen.

The invention discloses fragrant long-acting organic fertilizer based on sludge and lemon pomace. The organic fertilizer comprises the following components in parts by weight: 100 to 150 parts of sludge, 50 to 100 parts of lemon pomace, 10 to 15 parts of oil cake residue, 5 to 10 parts of agricultural nitric acid rare earth, 20 to 30 parts of waste oil, 5 to 10 parts of milk, 5 to 10 parts of vinasse and 0.5 to 1 part of a microbial agent; the total concentration of the finished living bacterial of the microbial agent is 1.5*10<13> cfu/ml; the lanthanum content of the agricultural nitric acidrare earth is 35 mg/kg and the cerium content of the agricultural nitric acid rare earth is 50 mg/kg; the pH of the sludge is 8 to 9; and the water content of a mixture of the sludge of the lemon pomace is 30 to 40 percent. The fertility of the soil is improved by combining the sludge and rich nitrogen phosphorus potassium elements in the lemon pomace; furthermore, the stench of the sludge is overcome and the ascaris eggs are killed to 100 percent; and the validity period is increased to 36 months.

The invention belongs to the technical field of porcine insect repellants and provides a preparation capable of preventing and treating ascaris suum and a preparation method. The preparation capable of preventing and treating ascaris suumcom comprises the following components in parts by weight: 13-16 parts of pumpkin seeds, 9-17 parts of nux vomica, 9-15 parts of groundsel, 6-9 parts of wikstroemia indica roots, 6-15 parts of quispualis indica, 13-20 parts of chinaberry root barks, 15-18 parts of gemma agrimoninae, 5-14 parts of lespedeza and 11-19 parts of pomegranate barks. The raw materials are cleaned, soaked, decocted, mixed and stirred to prepare the preparation capable of preventing and treating ascaris suum. The preparation capable of preventing and treating ascaris suum provided by the invention adopts a Chinese herbal medicine formula and makes full use of Chinese herbal medicines which are rich in alkaloid, so that the problems of drug resistance, medicinal residues, large toxic and side effects and poor effect are solved while ascaris suum is efficiently prevented and treated. Meanwhile, the invention provides a preparation method of the preparation capable of preventing and treating ascaris suum. According to the preparation method, active ingredients of Chinese herbal medicines capable of preventing and treating ascaris suum can be fully extracted, and relatively high activity is maintained.

The invention discloses anti-infection children fruit and vegetable flavor formula sheep milk powder and a preparation method thereof, and relates to the technical field of formula sheep milk powder. The formula sheep milk powder is prepared from raw sheep milk, demineralized whey powder, whole sheep milk powder, isomaltooligosacharide, concentrated whey protein powder, mixed vegetable oil, mixed fruit and vegetable powder, arachidonic acid, docosahexaenoic acid, N-acetylneuraminic acid, phosphatidylserine, yeast beta-glucan, fructus momordicae powder, phospholipid, potassium hydroxide and a compound nutrition enhancer. By adding the yeast beta-glucan, the reaction of immune cells of a determined population to bacterial infection is accelerated, and the position and speed of bacterial infection are rapidly controlled, so that the infection is relieved as soon as possible; and the formula sheep milk powder is capable of helping a human body to resist invasion of various pathogenic bacteria, such as staphylococcus aureus, escherichia coli, candida albicans, listeria monocytogenes, pneumocystis carinii, leishmania, herpes viruses, ascaris and the like, establishing a pathogenic bacteria defense line and perfecting an immune health system of children.

The invention relates to an anthelmintic suitable for an infant. The anthelmintic comprises the following compositions in parts by weight: 10-45 pumpkin seeds, 0.5-5 crystal sugar, 1-12 hawthorn, 5-25 sesame oil, 2-18 vinegar, 1-7 VC (Vinyl Chloride) and 40-60 water. In the anthelmintic suitable for the infant, the pumpkin seeds, the crystal sugar, the hawthorn, the sesame oil, the vinegar and the VC are taken as raw materials, wherein the pumpkin seeds have the effect of parasite expelling, and therefore, the anthelmintic can efficiently expel ascaris in the abdomen; the hawthorn has the action of stimulating the appetite, and therefore, the anthelmintic can relieve poor appetite caused by the ascaris in the abdomen; and the vinegar can take the effect of palsying the ascaris. The invention has the advantages of natural raw material acquisition, no toxic and side effects and good parasite expelling effect and is particularly suitable for the infant.

The invention belongs to the technical field of organic fertilizer fermentation, and discloses an organic fertilizer fermentation microbial agent and a preparation process. The preparation process is characterized by comprising the following steps of: S1, performing test tube culture; S2, performing shake-flask culture; S3, performing seeding tank culture: performing continuous aerobic culture on a culture solution I, a culture solution II and a culture solution III in a culture medium I, a culture medium II and a culture medium III respectively under the conditions that the inoculation amount is 0.5-1 percent and the temperature is 28-32 DEG C for 48 hours, and transferring into a fermentation tank for performing enlarged culture when proper conditions are met; S4, preparing a microbial carrier; and S5, drying the microbial carrier: smashing the microbial carrier through a sterile smashing machine to obtain a 120-mesh organic fertilizer fermentation microbial agent. The organic fertilizer fermentation microbial agent is cultured in a multi-step and classified way, so that the microbial growth index of various strains can be increased greatly, and the activity of each strain is increased greatly. When the fermentation microbial agent is used for preparing an organic fertilizer, nitrogen fixation, phosphorus dissolution, potassium dissolution, activation and decomposition of organic matters are realized, and escherichia coli and ascaris eggs are killed.

The invention relates to the technical field of digestion equipment, in particular to an enteroscope for removing intestinal ascaris, the enteroscope comprises a storage cavity, a storage box, a driving motor and a functional sleeve, and the functional sleeve, the driving motor, the storage cavity and the storage box are sequentially connected from left to right; a deinsectization pipe used for removing roundworms is connected in the functional sleeve in a sleeved mode, the outer circumferential face of the deinsectization pipe is attached to the inner circumferential face of the functional sleeve, one end face of the deinsectization pipe is coated with an adhesive used for adhering the roundworms, and the other end face of the deinsectization pipe is attached to the functional sleeve. A cyclone wheel disc which is driven by a driving motor to generate air flow to rotate is annularly arranged on the peripheral face of the end, provided with the adhesive, of the deinsectization pipe, the adhesive can well help the deinsectization pipe to adhere roundworms, and the problem that the roundworms are not easy to clamp is well solved.

The invention provides a roundworm mi RNA inhibitor. The nucleotide sequence of the inhibitor is: UCUACCCGAGAAUCGAACAGUC. Through combination with asu-miR-100 b, the inhibitor obviously reduces the abundance of asu-miR-100 b, significantly influences on the growth of the third-stage larvae of ascaris sum and reduces the infection rate. The asu-miR-100 b inhibitor expressed by the whole growth period of roundworm can be applied to influence the growth or functions related to its infectivity of the third-stage larvae of ascaris sum and ascaris lumbricoides.