Organic fertilizer fermentation microbial agent and preparation process

A fermentation agent and preparation technology, which is applied to the treatment of biological organic parts, bacteria, fungi, etc., can solve the problems of reduced activity of bacteria, shortening the time of fermentation and maturity, and reducing the number of turning times, so as to increase the number of bacteria Index, increase availability, increase the effect of specific surface area

Pending Publication Date: 2021-08-06
湖北金地天成农业发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The Chinese invention patent with application publication number CN112662593A discloses an organic fertilizer fermenting bacteria agent, which provides a method that can accelerate the fermentation and decomposing speed of organic waste, reduce the number of turning times, and shorten the time of fermentation and decomposing, but the microorganisms in this invention The fermentation method is not suitable for different types of bacterial agents. The organic microbial agent prepared by this method has low microbial activity, which leads to poor activation rate and various physical and chemical properties of subsequent organic fertilizers. The activity of the bacteria species decreases, and the number of bacteria increases slowly or Individual strains cannot survive, greatly reducing production efficiency

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  • Organic fertilizer fermentation microbial agent and preparation process

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Experimental program
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Effect test

Embodiment 1

[0036] S1: Test-tube culture: take the peeled potatoes and cut them into small pieces, boil them, filter the juice, add glucose and sterilize at 110-120°C for 30 minutes to obtain a standby solution 1; take beef extract, peptone, and sodium chloride to mix and dissolve and adjust the pH to 7.2-7.5, after sterilizing at a temperature of 120-125 ℃ for 30 minutes, the second liquid is obtained; take beef extract, peptone, yeast extract, diammonium hydrogen citrate, glucose, Tween, sodium acetate, dipotassium hydrogen phosphate, magnesium sulfate, Manganese sulfate, dissolve in water, adjust pH to 6.2-6.5, pack into laboratory anaerobic bottles, and sterilize at 120-125°C for 20 minutes to obtain standby solution III;

[0037] S2: Shake flask culture: Saccharomyces cerevisiae, Bacillus terpenoids, Aspergillus oryzae, Aspergillus niger, Staphylococcus thermophilus, Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus licheniformis are separately connected to the slanted strai...

Embodiment 2

[0042] S1: Test-tube culture: take the peeled potatoes and cut them into small pieces, boil them, filter the juice, add glucose and sterilize at 110-120°C for 30 minutes to obtain a standby solution 1; take beef extract, peptone, and sodium chloride to mix and dissolve and adjust the pH to 7.2-7.5, after sterilizing at a temperature of 120-125 ℃ for 30 minutes, the second liquid is obtained; after taking beef extract, peptone, yeast extract, diammonium hydrogen citrate, glucose, Tween, sodium acetate, and dipotassium hydrogen phosphate, add water to dissolve, Adjust the pH value to 6.2-6.5, pack into laboratory anaerobic bottles, and sterilize at 120-125℃ for 20 minutes to obtain the standby solution III;

[0043] S2: Shake flask culture: Saccharomyces cerevisiae, Bacillus terpenoids, Aspergillus oryzae, Aspergillus niger, Staphylococcus thermophilus, Bacillus subtilis, and Bacillus licheniformis were separately inserted into the standby solution 1 from the slanted strains of t...

Embodiment 3

[0048] S1: Test-tube culture: take the peeled potatoes and cut them into small pieces, boil them, filter the juice, add glucose and sterilize at 110-120°C for 30 minutes to obtain a standby solution 1; take beef extract, peptone, and sodium chloride to mix and dissolve and adjust the pH to 7.2-7.5, after sterilizing at a temperature of 120-125 ℃ for 30 minutes, the second liquid is obtained; after taking beef extract, peptone, yeast extract, diammonium hydrogen citrate, glucose, Tween, sodium acetate, and dipotassium hydrogen phosphate, add water to dissolve, Adjust the pH value to 6.2-6.5, pack into laboratory anaerobic bottles, and sterilize at 120-125℃ for 20 minutes to obtain the standby solution III;

[0049] S2: Shake flask culture: Saccharomyces cerevisiae, Bacillus terpenoids, Aspergillus oryzae, Aspergillus niger, Staphylococcus thermophilus, Bacillus subtilis, and Bacillus licheniformis were separately inserted into the standby solution 1 from the slanted strains of t...

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Abstract

The invention belongs to the technical field of organic fertilizer fermentation, and discloses an organic fertilizer fermentation microbial agent and a preparation process. The preparation process is characterized by comprising the following steps of: S1, performing test tube culture; S2, performing shake-flask culture; S3, performing seeding tank culture: performing continuous aerobic culture on a culture solution I, a culture solution II and a culture solution III in a culture medium I, a culture medium II and a culture medium III respectively under the conditions that the inoculation amount is 0.5-1 percent and the temperature is 28-32 DEG C for 48 hours, and transferring into a fermentation tank for performing enlarged culture when proper conditions are met; S4, preparing a microbial carrier; and S5, drying the microbial carrier: smashing the microbial carrier through a sterile smashing machine to obtain a 120-mesh organic fertilizer fermentation microbial agent. The organic fertilizer fermentation microbial agent is cultured in a multi-step and classified way, so that the microbial growth index of various strains can be increased greatly, and the activity of each strain is increased greatly. When the fermentation microbial agent is used for preparing an organic fertilizer, nitrogen fixation, phosphorus dissolution, potassium dissolution, activation and decomposition of organic matters are realized, and escherichia coli and ascaris eggs are killed.

Description

technical field [0001] The invention belongs to the technical field of organic fertilizer fermentation, in particular to an organic fertilizer fermentation bacterial agent and a preparation process. Background technique [0002] The organic fertilizer starter can decompose protein, cellulose, hemicellulose, lignin, etc., and compound bacteria and fungi. The organic fertilizer starter produced and developed by Beijing Huiminda Science and Technology Development Center has a high content of effective viable bacteria. It has strong degradation ability, and can achieve the effects of heating up, deodorizing, eliminating pests, weed seeds and improving nutrients at the same time. Under suitable conditions, it can quickly decompose and mineralize carbon, nitrogen, phosphorus, potassium, sulfur, etc. in the pile to form simple organic matter, which is further decomposed into nutrients that can be absorbed by crops. [0003] The Chinese invention patent with the application publica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N1/18C05F17/20C12R1/865C12R1/07C12R1/69C12R1/685C12R1/645C12R1/125C12R1/10C12R1/01C12R1/23C12R1/225C12R1/46
CPCC12N1/20C12N1/14C12N1/18C05F17/20Y02W30/40
Inventor 徐广朝李春映
Owner 湖北金地天成农业发展有限公司
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