The invention discloses a chitosan oligosaccharide composite with cold-resistant function, which is aqueous solution, wherein the mass concentration of the chitosan oligosaccharide is 5%-10%, other auxiliary active constituents include at least one of phosphorus, potassium, calcium, boron, molybdenum, zinc, salicylic acid and betaine, and the content of the auxiliary active constituents is 0.5%-30% of the total mass concentration of the composite. Before the flowering period and the young fruit period of fruit trees, the time of young seedling of melon and fruit, vegetables and flowers, and the cold sensitive period of the winter growth of greenhouse crops, the composite is diluted by 1000 times with water to be sprayed on the leaves of the plants for 2-3 times; the chitosan oligosaccharide composite used in the invention has the function of promoting photosynthesis of the crops, improving activation performance of various enzymes and promoting metabolism of carbohydrates; and the chitosan oligosaccharide composite is used for inducing the crops to improve stress resistance and inducing the crops to improve cold resistance, so that the crops can get through the low-temperature cold harmful period, and regular development and yield of the crops are ensured.