23 results about "Mass propagation" patented technology

The present application relates to a kind of methods for mass propagation of tea corner miridae in laboratory, comprising the following technical steps: collecting tea garden tea corner miridae adult, cultivating fall kui seedling, putting seedling and leading insect, tea corner miridae habitat and feeding, mating and oviposition and nymph hatching and feeding.The present application uses fall kui seedling as the main body of feeding raw material, and the leaf of raw material is relatively thick, strong in harm resistance, not easy to dry, which helps to improve the hatching rate of tea corner miridae eggs, reduce the frequency of raw material supply and replacement, at the same time, the present application saves time and effort, can be realized in laboratory low cost and sustainability fast, efficient, mass propagation of tea corner miridae test insect source.

The invention relates to the technical field of agricultural planting, in particular to a straw returning optimized fertilization method. The method comprises the following steps: cutting corn straws into small sections, and carrying out air drying treatment; then, the air-dried corn straw is flatly laid at the 20-30 cm position of the planting field; after straw returning to the field, base fertilizer is applied in combination with ploughing operation. The corn straw is cut into sections and air-dried, so that the contact area between the straw and microorganisms is increased, attachment and decomposition of the microorganisms are facilitated, and a proper living environment is created for the microorganisms. Base fertilizer is applied in combination with cultivated land after straw returning, on one hand, mass propagation of microorganisms is promoted, and the straw decomposition capacity of the microorganisms is enhanced; on the other hand, the straw, the soil and the base fertilizer are fully mixed, nutrients generated by straw decomposition are evenly distributed in the soil and absorbed and utilized by corn roots in time, the problems that straw decomposition is slow and the corn competes for nutrients and water are solved, normal growth of the corn is guaranteed, effective utilization of the straw is achieved, and good promotion of corn growth is achieved.

The invention discloses a micro-ecological flora in-situ screening and anaerobic acid production enrichment method and device based on selective metal ion removal, belongs to the field of anaerobic treatment of high-concentration organic wastes, and solves the problems of coordinating the flora relationship of the organic wastes and improving the enrichment efficiency of anaerobic produced short-chain fatty acids. The method comprises the following steps: gradually removing soluble Fe, Co, Ni, Mg and Ca in an exchangeable state and a carbonate binding state from organic wastes by using a filler loaded with chelating groups, creating a multivalent metal ion deficiency environment, and stresses the evolution of a micro-ecosystem by using the selectivity dependence difference of acid-producing fermentation flora and methanogenic flora on metal ions, so as to achieve the purpose of improving the yield of the organic wastes. The interaction relationship between the multivalent metal and the acidogenic fermentation flora and methanogenic flora is regulated and controlled, the flora screening effect is exerted, the acidogenic fermentation flora is enriched, the methanogenic flora is inhibited, and the acidogenic fermentation flora is promoted to become dominant bacteria for mass propagation and proliferation, so that the acidogenic reaction is improved and the methanogenic reaction is inhibited; the efficiency improvement and the lossless enrichment of anaerobic acid production of the high-concentration organic waste are realized.

The present application provides a method for reconstructing wild population of Taxus chinensis var. mairei, wherein a seedling cultivation technique is simple and achieves a high seed germination rate, enabling mass propagation of high-quality seedlings to supply sufficient seedling materials for the wild reintroduction of Taxus chinensis var. mairei; a hardening-off technique enhances stress resistance of the seedlings and shortens the cultivation cycle required for the seedlings to reach standard seedling specifications; a wild acclimatization technique improves adaptability of the seedlings and elevates quality of the Taxus chinensis var. mairei seedlings; both a reintroduction site habitat restoration technique and a wild reintroduction technique help to improve survival rate of the Taxus chinensis var. mairei. By means of the population reconstruction process comprising the seedling cultivation, the hardening-off and wild acclimatization, the reintroduction site habitat restoration, and the wild reintroduction, survival rate of the Taxus chinensis var. mairei is improved, thereby successfully reconstructing the wild population of Taxus chinensis var. mairei.

The invention provides a vibrio parahaemolyticus bacteriophage RKP-VP23248 and application of the vibrio parahaemolyticus bacteriophage RKP-VP23248, the vibrio parahaemolyticus bacteriophage is preserved in the China General Microbiological Culture Collection Center on September 27, 2024, and the preservation number of the vibrio parahaemolyticus bacteriophage RKP-VP23248 is CGMCC (China General Microbiological Culture Collection Center) No. 46193. The invention further provides a bacteriophage composition, and the bacteriophage composition comprises the vibrio parahaemolyticus bacteriophage RKP-VP23248. The bacteriophage provided by the invention is separated from natural water environments such as seawater, river water and water sediment, still keeps high-level titer under continuous passage, has relatively strong genetic stability and thermal stability, and can effectively cope with disease outbreak caused by mass propagation of pathogenic bacteria when water environment conditions change. The bacteriophage has strong lysis property on the drug-resistant high-pathogenicity vibrio, the lysis rate is up to 88.51%, and the problem of vibrio drug resistance enhancement caused by antibiotic abuse can be effectively solved, so that the problems of large-scale acute death and the like of aquatic animals such as fishes, shrimps and the like caused by the high-pathogenicity vibrio are solved.

The invention belongs to the field of plant planting, and particularly relates to a method for improving the germination rate of papaya seeds and cultivating high-quality seedlings. Comprising the following steps: (1) seed selection: harvesting seeds on amphoteric plants when fruits are mature, taking out the seeds, removing aril, cleaning with clear water, airing, and selecting full seeds; (2) seed pretreatment; (3) peeling off testa; and (4) sowing the treated seeds in a seedling raising tray, and raising seedlings according to a conventional seedling cultivation method. According to the method, the germination rate of the papaya seeds is increased to 98.5% for the first time, the seedling time is shortened, germination and seedling emergence are neat, seedlings are thick and strong, root systems are developed, and the seedling quality is high. The method solves the problem of low germination rate or long germination time of papaya under conventional conditions, is an improvement of a papaya seedling culture key technology, and provides a simple and practical method for mass propagation of papaya seedlings. The method has the advantages of being simple in treatment process, low in cost, easy to operate and the like.

The invention provides sour tea with a hypoglycemic effect and a preparation method thereof, and the preparation method comprises the following steps: S1, preparing an organic fertilizer, and planting tea seedlings by using the organic fertilizer; s2, after the tea tree grows to be mature, picking fresh leaves with one bud and two leaves or one bud and three leaves and uniform in size; s3, the picked fresh leaves are subjected to withering, fixation and rolling in sequence, and tea leaves are obtained; s4, mixing the tea leaves obtained in the step S3 with brown sugar, and performing anaerobic fermentation in a closed environment; and S5, tedding and airing the tea leaves subjected to anaerobic fermentation to obtain the sour tea. According to the method, the tea leaves and the brown sugar are mixed and then subjected to anaerobic fermentation, in the anaerobic fermentation process, the brown sugar serves as an important nutrient substance, fermentation substrates are increased, energy is provided for mass propagation of microorganisms, rapid fermentation of the microorganisms is promoted, effective microbial communities are generated, the nutritional quality of the sour tea is further improved, and the prepared sour tea has a certain hypoglycemic effect.

A system and method for growing microalgae capable of mixotrophic metabolism, preferably Chlorella sp. Microalgae grown in the system using the method are able to survive and grow in dark refrigeration, which allows the algae to be stored and transported for application as a live culture. In addition, the microalgae can be grown in sufficient quantities to be sold commercially for application to crops as a biostimulant.

The method comprises the following steps: (1) collecting branches of the purple gynura, trimming and disinfecting the branches, and picking off stem tops to serve as explants; and (2) carrying out primary culture, subculture and rooting culture on the explants, a culture medium used in the primary culture comprises 1.5-2mg / L of B5 + 6-BA, 0.1-1mg / L of NAA, 25-30g / L of cane sugar and 9g / L of agar, and the pH value is 5.8; a culture medium for subculture is prepared from B5, 0.3 mg / L to 3mg / L of furfuryl aminopurine, 0.01 mg / L to 1mg / L of NAA, 30g / L of cane sugar and 6.5 g / L of agar, and the pH is 5.8; a culture medium for rooting culture is B5, 1-10mg / L of furfuryl aminopurine, 1mg / L of IBA, 30g / L of cane sugar and 6.5 g / L of agar, and the pH is 5.8. According to the method disclosed by the invention, the branches of the gold-leaf purple gynura are used for culturing, so that rapid and large-scale proliferation of the gold-leaf purple gynura can be realized, and the problem that the division and cutting propagation speed of the gold-leaf purple gynura is relatively low is solved. The method is simple and convenient to operate and more suitable for production practice.

The invention provides a Shewanella algal bacterium phage (Shewanella algal bacterium phage) PKP-SH23103 and application of the Shewanella algal bacterium phage. The Shewanella algal bacterium phage is preserved in the China General Microbiological Culture Collection Center on November 29, 2023, and the preservation number is CGMCC (China General Microbiological Culture Collection Center) NO.45783. The invention further provides an application of the Shewanella algal bacterium phage PKP-SH23103. The invention further provides a bacteriophage composition, and the bacteriophage composition comprises the algal shewanella bacteriophage PKP-SH23103. The phage provided by the invention is separated from a natural water environment, still keeps high-level titer (1010PFU / mL) under continuous passage, has relatively strong genetic stability and thermal stability, has the characteristic of high permeability resistance, and can effectively cope with disease outbreak caused by mass propagation of pathogenic bacteria when water environment conditions change; the bacteriophage obtained by the invention can be used for preventing and treating diseases caused by shewanella alginate, is safe and environment-friendly, effectively replaces the use of antibiotics in a culture process, reduces the problems of water body pollution, enhanced bacterial drug resistance, drug residues and the like caused by antibiotic abuse, and has good practical application value.

The invention discloses a culture medium combination for tissue culture and breeding of asparagus fern. The culture medium combination consists of an explant culture medium, an axillary bud and cluster bud induction culture medium, an axillary bud and cluster bud proliferation culture medium, rooting water and a rooting culture medium. By means of breeding of the culture medium combination, the pollution rate of the asparagus fern explant can be controlled within 10%, the germination rate of the explant is higher than 80%, the first rooting rate reaches 60% or above, the second rooting rate reaches 90% or above, the culture medium combination can be directly used for factory production of asparagus fern tissue culture, and a solid foundation is laid for mass propagation of asparagus fern.

The invention discloses a delphinium ajacis lateral bud mass propagation method, and belongs to the technical field of agricultural plant tissue culture propagation. According to the method, the delphinium ajacis lateral buds are used as explants, delphinium ajacis seedlings with stable characters are produced through a tissue culture method, and the propagation cardinal number of delphinium ajacis is rapidly increased. The phenomenon that the characters are not fixed when a sexual propagation method is adopted for expanding propagation is prevented. And the breeding speed of the delphinium ajacis can meet the market requirement.

The application belongs to the technical field of plant tissue culture and particularly relates to a tissue culture propagation method of Cupressus gigantea. In order to solve the technical problems of difficult asexual reproduction, low seed germination and rooting rates of Cupressus gigantea, the application provides the tissue culture propagation method of Cupressus gigantea, which comprises the following steps: taking Cupressus gigantea seeds or branches as explants, sterilizing the explants, and then sequentially performing initiation culture and rooting culture; when the Cupressus gigantea seeds are taken as the explants, the initiation culture further comprises proliferation culture. The application obtains the optimal tissue culture condition of Cupressus gigantea by screening the sterilization mode, the initiation culture medium, the proliferation culture medium and the rooting culture medium, and the survival rate of transplanting is more than 90% after seedling transplanting and local transplanting. The application provides technical support for the mass propagation of Cupressus gigantea and has important significance for the field return and population recovery of Cupressus gigantea.

The utility model discloses a water culture and soil culture shared device for cutting plants. The water culture and soil culture shared device comprises a lower water culture cup (1) and an upper soil culture cup (2), the cup bottom of the upper soil culture cup (2) is connected with the cup opening of the lower soil culture cup (1); the interior of the cup bottom of the upper soil culture cup (2) protrudes upwards to form a conical body (21), and a through hole (22) is formed in the top of the conical body (21); a drain hole (24) is formed in the edge of the joint of the bottom edge and the cylinder body (23) of the upper soil culture cup (2); the cuttage branches (001) are inserted into the lower soil culture cup (1) through the through holes (22) in the upper soil culture cup (2); and a sealing body (5) is arranged at the through hole (22). Water cutting and soil cutting are carried out at the same time, water cutting provides nutrition for cutting branches, and meanwhile soil cutting is used for improving the follow-up survival rate. Therefore, the problems that direct soil cutting is not easy to root, nutrition growth of water cutting is not vigorous than that of soil culture, and root rotting and death are easy to occur when water cutting is converted into soil culture can be solved, and the method is suitable for mass propagation.

The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a cluster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant miscanthus), and their uses in propagating Miscanthus x giganteus (Giant miscanthus).

The application discloses a culture medium combination for tissue culture of Asparagus cochinchinensis, which is composed of an explant culture medium, an axillary bud and cluster bud induction culture medium, an axillary bud and cluster bud proliferation culture medium, rooting water and a rooting culture medium. Through the tissue culture of the culture medium combination, the explant contamination rate of Asparagus cochinchinensis can be controlled within 10%, the explant budding rate is higher than 80%, the first rooting rate is higher than 60%, and the second rooting rate is higher than 90%, so that the culture medium combination can be directly used for factory production of Asparagus cochinchinensis tissue culture, and lays a solid foundation for mass propagation of Asparagus cochinchinensis.

The utility model relates to the technical field of pomacea canaliculata physical prevention and control, aims to solve the problems that in the prior art, the egg killing efficiency is low, the environment is prone to being polluted, the prevention and control effect is slow, mass propagation of pomacea canaliculata cannot be prevented, and the local ecological environment cannot be rapidly recovered, and provides a pomacea canaliculata egg killer which comprises a telescopic rod. The top end of the telescopic rod is detachably connected with a rolling device used for grinding ampullaria gigas eggs, the rolling device is provided with a connecting frame, the end, close to the telescopic rod, of the connecting frame is detachably and fixedly connected to one end of the telescopic rod, the end, away from the telescopic rod, of the connecting frame is provided with a rotatable roller, and the outer wall of the roller is provided with a plurality of spikes. And the plurality of spikes are uniformly distributed on the outer wall of the roller. The pomacea canaliculata egg killing device has the advantages of being high in egg killing efficiency, free of environment pollution, fast in prevention and treatment effect and capable of rapidly preventing the pomacea canaliculata from mass propagation and rapidly recovering the local ecological environment.

The invention discloses a preparation method of nano-metal drug-loaded particles for seed treatment and application of the nano-metal drug-loaded particles in prevention and control of rice diseases and insect pests. According to the invention, the nano-carrier is prepared from the organic metal framework and is loaded with the pesticide, and the tannic acid metal complex shell is assembled, so that targeted controlled release of the pesticide can be carried out at harmful organism hazard parts, and mass propagation of pathogenic bacteria under an acidic condition and harm of pests are effectively coped with. The nano-metal drug-loaded particles show an excellent targeted pest control effect, the effective utilization rate of pesticide active ingredients is increased, the lasting period is prolonged, germination of rice seeds and growth of rice seedlings are promoted, and the nano-metal drug-loaded particles are particularly suitable for improving the quality of the rice seedlings in direct seeding cultivation of the rice and have a wide rice seed treatment application prospect.

The utility model discloses a spartina alterniflora harvester which comprises a main machine body in which an engine is arranged, and further comprises a cab arranged on the main machine body; the conveying assembly is obliquely arranged on the main machine body, is close to the cab and comprises a header conveying belt and a first supporting frame, one end of the first supporting frame is connected with the conveying assembly, the other end of the first supporting frame is connected with the main machine body, and the feeding end of the header conveying belt is lower than the discharging end of the header conveying belt; the cutting assembly is arranged at the feeding end of the conveying assembly, is used for cutting spartina alterniflora and comprises a cutting knife and a collecting roller, the cutting knife is connected with the collecting roller, and the collecting roller is connected with the header conveying belt. By arranging the collecting box, grass seeds of the spartina alterniflora can be prevented from leaking to the mud flat and being transported along the way, mass propagation of the spartina alterniflora can be avoided, the header conveying belt and the conveying belt in the collecting box are made of stainless steel grid materials, the spartina alterniflora cut by the cutting assembly is drained through the stainless steel grid conveying belt, and the weight of the spartina alterniflora is reduced; and the resource consumption is reduced.

The invention discloses an artificial breeding anoplophora chinensis biological type dastarcus helophoroides and a method for preventing and treating anoplophora chinensis, which comprises the following steps of: firstly, collecting wild anoplophora chinensis dastarcus adults in the field, feeding and inducing oviposition, storing the oviposition grains, taking out part of the oviposition grains to incubate the anoplophora chinensis biological type dastarcus helophoroides one-year-old larvae, and taking out part of the oviposition grains to incubate the anoplophora chinensis biological type dastarcus helophoroides; the other part of egg grains can be used for manufacturing egg cards for field release for preventing and treating anoplophora chinensis. Then, anoplophora chinensis larvae or barley pest pupae are utilized to breed first-instar larvae of anoplophora chinensis biological type dastarcus helophoroides, and adults are emerged and stored; and finally, part of imagoes are used for repeating the process, and large-scale artificial breeding of the biological dastarcus helophoroides of the anoplophora chinensis is achieved. The other part can be used for field release for preventing and treating anoplophora chinensis. According to the method, biological prevention and control are carried out by utilizing parasitic natural enemy anoplophora chinensis biological type dastarcus helophoroides, the anoplophora chinensis is finally killed through artificial mass propagation and release, and the quantity of pest populations can be controlled at a relatively low level when the anoplophora chinensis is continuously released.

The invention provides a tissue culture medium for rapid propagation of caulis sinomenii and a method for rapid propagation of caulis sinomenii, and belongs to the technical field of plant tissue culture. The invention provides a tissue culture medium for rapid propagation of caulis sinomenii. The tissue culture medium comprises an induction medium and a rooting medium, the induction culture medium takes an MS culture medium as a basic culture medium, and further comprises 1-2mg / L of 6-BA and 0.1-0.2 mg / L of NAA; the rooting culture medium takes a 1 / 2 MS culture medium as a basic culture medium, and further comprises 0.1-1g / L of LAc and 0.1-0.2 mg / L of NAA. According to the embodiment of the invention, stem segments with buds of caulis sinomenii are taken as explants, axillary buds are directly induced to differentiate by utilizing the tissue culture medium, a large number of seedlings can be obtained, mass propagation can be realized within a short time, and the propagation efficiency of caulis sinomenii is improved.

The application discloses a culture method for propagating Brachythecium populeum, and belongs to the technical field of Brachythecium populeum tissue culture. The application uses sporangium as an explant to propagate Brachythecium populeum, and quickly obtains Brachythecium populeum spores with a high germination rate, establishes a regeneration system of Brachythecium populeum, lays a foundation for large-scale propagation of Brachythecium populeum, shortens the propagation cycle of Brachythecium populeum, and better maintains excellent properties. The application first establishes a method for culturing Brachythecium populeum by using spores, fills the blank of the prior art, and provides a technical basis for propagation, seed preservation, preparation of protoplasts, recombination experiments and gametophyte culture of Brachythecium populeum.

The invention belongs to the technical field of deep processing of agricultural products, and provides a production method of probiotic pickles, which is characterized in that fresh vegetables are fermented by adding specific probiotic strains to produce the probiotic pickles. The pickled vegetables added with the probiotics can specifically promote mass propagation of strains with high acid resistance such as lactobacillus acidophilus, lactobacillus plantarum and lactobacillus brevis, and the strains can consume a large amount of oxygen and effectively inhibit growth of infectious microbes, especially aerobic harmful bacteria. They not only can survive and colonize in the stomach, but also can resist the invasion of helicobacter pylori. More importantly, the probiotics can pass the test of gastric juice by 100%, can be planted into the large intestine, and can continue to consume oxygen and prevent the growth of harmful bacteria, thereby maintaining the health of the intestinal tract.