359 results about "Mass propagation" patented technology

The invention discloses a method for artificially feeding black soldier flies, which comprises the following steps of: feeding adults, collecting eggs and incubating, feeding larvae, pupating and the like. The provided method for artificially feeding black soldier flies is a mature method for artificially feeding black soldier flies. The method is easy and effective, is easy to operate, is suitable for mass propagation and production of the black soldier flies, and lays a foundation for effectively utilizing the black soldier flies, such as treating refuse, changing waste into treasure or using larvae as feeds or baits.

The invention discloses a method for propagating Phytoseiulus Persimilis, which comprises the steps: planting and cultivation of host plant, inoculation and feeding of Tetranychus cinnabarinus, inoculation and feeding of Phytoseiulus Persimilis as well as collection, package and refrigeration of Phytoseiulus Persimilis. The method takes mass propagation of Phytoseiulus Persimilis as the purpose, provides technical support for feeding Phytoseiulus Persimilis on scale, and provides special predacity natural enemy for Tetranychus pest mites on greenhouse crops for biological control. The invention is a production method in which living hosts (firstly host plant and host Tetranychidae are cultivated) are adopted for feeding Phytoseiulus Persimilis, and makes a series of breakthroughs on key techniques for artificial mass propagation of Phytoseiulus Persimilis. The method of the invention can lead the production cost of Phytoseiulus Persimilis to be stabilized, the production period to be 25 days, and the production scale to reach 10,000/m<2>.

The present invention discloses a fully soluble aquatic fertilizer and its preparation method and pertains to aquaculture field. The aquatic fertilizer of the present invention comprises monoammonium phosphate 15-45%, sodium humate 12-20%, sodium silicate 5-12%, compound premix 8-13%, and urea 30-40%. The compound premix is mainly composed of trace elements. Firstly, the aquatic fertilizer of the present invention has comprehensive nutrition and balanced formula; secondly, the present invention has high dissolution speed and utilization rate; thirdly, the present invention features simple use method, small use amount, sufficient content, long lasting time and low cost; fourthly, the present invention can not only promote the mass propagation of food organisms of aquatic animals but also specifically cultivate helpful algae, improve water quality, increase dissolved oxygen, effectively avoid pond poisoning, reduce diseases, lower feed coefficient and enhance fish immunity; the present invention is green and environment-friendly, and has stable quality and no harm on human and animal and no pollution on environment.

The invention relates to a livestock and poultry farm dung dirt biological fermentation treatment method and a livestock and poultry farm dung dirt biological fermentation treatment device. The method comprises the following steps of: hardening and tempering the collected dung dirt, and spraying the dung dirt into a backing in a fermentation workshop through a pipeline system by using theories of microbial occupying, aerobic fermentation, three channel operation and the like; thoroughly digesting and decomposing farm dung dirt by using highly active enzymes generated by mass propagation of beneficial microorganisms which are mainly bacillus natto in the backing; and evaporating water in the dung dirt by using a great amount of biological heat generated in the backing in a microbial activity process so as to realize zero emission of the farm dung dirt.

The invention relates to a composite microbial inoculant and its preparation method, and overcomes the problems of slow decomposing agent start and incomplete decomposition in the prior art. The invention provides a straw decomposing agent for quick start of decomposition at low temperature, and the straw decomposing agent comprises a strain YO-H, bacillus subtilis, Saccharomyces cerevisiae, Aspergillus niger and Trichoderma harzianum. The straw decomposing agent provided by the invention has the advantages that: 1. it employs the strain YO-H, and can quickly start decomposition under the condition of a low environmental temperature; 2. the strain YO-H cooperates with other decomposing fungi to achieve a better decomposing effect; and 3. an additional enzyme preparation is added to improve the cellulose activity of the decomposing agent, about 3-4 days are left before mass propagation of decomposing fungi and generation of new enzyme, and during the period, the additionally added enzyme preparation is employed to degrade lignin and cellulose, that is to say the decomposition time is 3-4 days ahead of schedule.

The invention discloses a method for feeding lepidopteron. In the feeding process, a tray type net cage is used as a main feeding tool. The method comprises steps of collecting eggs, sterilizing, hatching larvae from the eggs, feeding the larvae, feeding pupas, feeding imagoes and the like, wherein gauze or plastic ropes are used as an oviposition substrate for collecting the eggs; the eggs are sterilized by using a formaldehyde solution with the volume concentration of 3-4%; the eggs are hatched and fed in an illumination incubator; the larvae are sterilized by using a sodium hypochlorite solution with the volume concentration of 8-12%; pupas feather in the tray type net cage; the feathering imagoes are continuously fed in the net cage and the eggs are collected and sterilized. The tray type net cage is simple and convenient to operate, the insects are effectively prevented from escaping and the cleaning can be carried out at any time; in the feeding process, eggs and larvae are disinfected by using different disinfectants, the disinfection effect is thorough and gentle, the bacteria are effectively prevented from mass propagation, and the hatchability of the eggs, the feathering rate of pupa are also improved, the hatchability of the eggs can be 95-96%, and the feathering rate of pupa can be 98%.

The invention discloses a composition for removing acnes and application of the composition in cosmetics. The composition is prepared from the following components: a paeony extract, a cucumber extract, a feverfew extract, a yellowish sophora root extract, a hamamelis virginiana extract, a lonicera tragophylla extract, tea tree leaf oil and oligosaccharide. The composition disclosed by the invention further comprises one or the combination of several of nicotinamide, panthenol, allantoin and oat kernel oil. The composition is suitable for acne type skins and helps users resist and relieve inflammation caused by exuberant sebum secretion, sebaceous gland obstruction and mass propagation of propionic acid acne bacillus and to repair the skin. According to the application of the composition for removing the acnes in medicines or cosmetics, the composition can be made into liquid, gel, emulsion or paste and other forms.

The invention provides a novel composite starter for pickled cabbage, which belongs to the biotechnical field, and is used for solving the problems of pickled cabbage deterioration caused by growth environment change-induced massive cell death, bacteria sharp cutoff-induced difficulty in rapid decrease of initial pH value in fermentation, and mass propagation of various microorganisms during natural fermentation or inoculated fermentation. The composite starter is composed of three kinds of lactobacillus and a protectant, wherein a ratio of Leuconostoc mesenteroides to Lactobacillus plantarum to Lactobacillus casei is 1:1:(0.7-1.3); a ratio of the protectant to lactobacillus is 3:(0.8-1.2); and the protectant consists of the following raw materials: 1.5% of trehalose, 0.5% of glycerol, 2% of sorbitol, 1% of maltodextrin and 10% of skimmed milk. A preparation method of the composite starter comprises pre-refrigerating at minus70 DEG C for 2-3hr, and performing freeze drying in vacuum. The inventive composite starter can well restore the flavor of the conventional pickled cabbage. The pickled cabbage produced by using the composite starter is mellow, fresh and tasty, and has moderate sourness and pure flavor.

The invention belongs to the field of animal cultivation, and particularly relates to a ricefield eel culturing method. According to the ricefield eel culturing method, the surviving rate, growth speed and quality of ricefield eels are high. The ricefield eel culturing method includes the following steps that a pond which is good in water quality, free of pollution and quiet is selected; the pond is cleared up manually for clearing redundant sludge, and sludge with the depth of 20cm remains; thorough disinfection is carried out on the pond through quick lime, water is changed thoroughly once after the disinfection, and water grass is planted in the pond; earthworms are thrown in the pond and perform mass propagation after about 20 days; ricefield eel fries are put in the pond; in the process of ricefield eel culturing, fish bait is thrown according to the amount of the earthworms in the pond, fish bait throwing is not needed if the earthworms are sufficient, if the earthworms are not sufficient, the fish bait is thrown for 1-3 times every day according to conditions, and the fish bait mainly contains little fish, fly maggots, yellow mealworms and the like; after cultivation for one and half years, the ricefield eels are fished for sell through a fishpot.

The invention discloses a method for preparing bacteria-free pine wood nematodes. The method for preparing the bacteria-free pine wood nematodes comprises the following steps: culturing the pine wood nematodes; preparing pure pine wood nematode eggs; soaking the pine wood nematode eggs in 15% H2O2 for 50-60 minutes; incubating the pine wood nematodes; and culturing the pine wood nematodes and performing bacteria-free identification, thereby preparing the bacteria-free pine wood nematodes. As for the method for preparing the bacteria-free pine wood nematodes, the property of being capable of being adhered on a glass vessel of the pine wood nematode eggs is utilized, a small culture dish is adopted for collecting the eggs, the newly prepared 15% H2O2 is further used for performing sterilization treatment, the bacteria-free pine wood nematode eggs are obtained, the bacteria-free eggs are incubated, mass propagation is further performed on botrytis cinerea, and then the bacteria-free pine wood nematodes are finally obtained. Under sterile conditions, the obtained bacteria-free pine wood nematodes are used for performing an inoculation test on seedlings cultured by spruce bacteria-free tissues, and the result shows that the inoculation of the bacteria-free pine wood nematodes can still enable the bacterial-free seedlings to wilt. The method can be adopted for enabling the pine wood nematodes to be bacteria-free and lose pathogenicity, thereby being the simple and successful method.

The invention relates to a method and a system for producing biodiesel by cultivating microalgae by using urban sewage. According to the method and the system, a sewage treatment system is converted from a treatment technique to a production technique; high contents of organics and nutrients such as nitrogen and phosphorus in the urban sewage are conducive to mass propagation of the microalgae, so that the sewage is converted from pollutants to raw materials for the growth of the microalgae; CO2 circulation and O2 circulation are tightly combined through the establishment of an algal-bacterial symbiotic system, so that the growth of the microalgae is no longer dependent on an external carbon source, and meanwhile, the production cost is low due to the reduction of aeration systems in an aeration tank; the illumination in the aeration tank is abundant due to the use of a photosynthesis component, which is conducive to the photosynthesis of the microalgae; and the subsequent sludge quantity is small due to the use of efficiently-degraded strains.

The invention discloses a method for treating electroplating wastewater. The method comprises the following steps: (1) primary physical-chemical treatment; (2) secondary physical-chemical treatment: after the discharged water generated in step (1) is subject to homogenizing treatment, adding sodium hypochlorite into the homogenized water, then adding sodium pyrosulfite into the obtained product to remove residual chlorine, and adding aluminum chlorhydroxide into the obtained product; and (3) biochemical system treatment: adding appropriate biochemical sewage into the product obtained in step (2), mixing the biochemical sewage and the product, then feeding the obtained mixture into a biological hydrolysis pool; controlling the mixture under appropriate conditions and within an appropriate retention time; carrying out reduction on the obtained mixture by utilizing facultative anaerobes; and hydrolyzing the obtained discharged water subjected to reduction, feeding the discharged water into an aerobic biological oxidation pond, and then carrying out mass propagation of engineering bacterias under an appropriate condition by using organic pollutants in wastewater. By using the method provided by the invention, the total treatment rates of a system are as follows: the treatment rate of heavy metal is greater than or equal to 99.7 percent, the treatment rate of ammonia nitrogen is greater than or equal to 90 percent, and the treatment rate of chemical oxygen demand (COD) is greater than or equal to 88 percent, therefore, the electroplating wastewater treated by using the method comprehensively meets the requirements of the Discharge Standard for Pollutants from Electroplating Industry (GB21900-2008).

The invention belongs to the field of wine brewing and particularly relates to a preparing method and application of pit mud for improving the flavor of baijiu in a new pit. The preparing method comprises the steps of preparation, and primary fermentation and secondary fermentation of raw materials. According to the preparing method, microorganisms in yellow-water-aged pit mud diluent are used for mass propagation in primary pit mud, starter microorganisms are properly added, and thus comprehensive composite pit mud function bacteria are obtained and replace a single strain to serve as a microorganism species source. Meanwhile, yellow-water-aged pit mud diluent is used for long-time soaking, a small amount of foreshot and after-run are supplemented, and thus certain organic acid and alcohol are increased, so that the original ecology of aged pit mud is simulated, and thus microorganism fermentation is better promoted. Black peat is further added to serve as humus and replace urea added by other factories as a nutrition source, and thus ethyl carbamate is avoided. On the aspect of pit mud aging, the prepared pit mud and the aged pit mud are mixed at the bottom of the pit, fermented mass fermentation is carried out for multiple turns to promote aging of the new pit mud, the circle is short, and higher practicability is achieved.

The invention relates to the technical field of field acquisition, artificial domestication and mass propagation of insects and in particular discloses a domestication and culture method and an application of hermitia illucens WH. The eggs and adults of Wuhan wild hermitia illucens are acquired and respectively raised and domesticated, thus obtaining the stable artificial propagation strain and creating a technology of mass raising. The hermitia illucens WH larvae raised in mass are utilized to transform pig manures, chicken manures and cattle manures respectively and accumulation of livestock and poultry manures can be reduced by more than 50% through treatment. The obtained prepupa contains 35-39% of crude protein, the protein is formed by 17 amino acids, the prepupa contains 33-35% of fat and the fat can serve as the high-quality feed ingredient and nutrition. The domestication and culture method has the following advantages and beneficial effects: the hermitia illucens larvae are utilized to transform livestock and poultry manures; the method has the advantages of simpleness and convenience in operation, deodorization, pathogenic bacteria removal, zero energy consumption, easiness in popularization and the like; and the environmental pollutants are effectively utilized and the high added value product can be obtained.

The present invention relates to a method for mass propagating the adventitious root of ginseng, camphor ginseng, wild ginseng by tissue culture and improving the saponin content. More specially, the method comprises the steps of: tissue culturing of the leaf, root, stems of ginseng, camphor ginseng, wild ginseng and dissecting the callus; Propagating the adventitious root, which was developed by above dissected callus; Mass culturing of the propagated adventitious root in a bioreactor. Especially, the present invention makes it possible to produce the adventitious root that has enriched saponin content and the ratio of diol saponin and triol saponin being the same level of the natural ginseng, thereby, it can provide more valuable adventitious root in commercial and usefulness.

The invention discloses a microorganism soil activator, relates to soil improvement materials, and belongs to the technical field of agriculture and biological engineering. The microorganism soil activator is characterized in that the microorganism soil activator comprises, by weight, 5-10% of microorganism cultures, 10-25% of potatoes, 15-35% of straw, 50-70% of organic fermented materials, 3-7% of xanthan gum, 5-15% of humic acid, 0.05-0.2% of ammonium di-hydrogen phosphate and 0.1-0.5% of soil disinfectant. The beneficial effects of the microorganism soil activator are that: the microorganism soil activator is mainly used to improve poor soil, metabolites generated due to mass propagation of microorganisms can promote the formation of soil granules, soil hardening due to a condition that a great amount of fertilizer is used in successive years is prevented, the soil structure and ecological environment are improved, and the yield and quality of crops are improved.

The invention discloses special EM (Effective Microorganisms) bacteria powder for aquaculture, as well as a preparation method and application of the special EM (Effective Microorganisms) bacteria powder. Bran, corn flour, amino acid powder and the like, which are used as dry matter fillers, are mixed with brown sugar, glucose and the like to obtain a mixture; the mixture is subjected to sealing fermentation at 25-37 DEG C for about 10 days by lactic acid bacteria, bacillus licheniformis and bacillus coagulans, so that fermented materials are prepared; then the prepared fermented materials are dried and crushed and are mixed with bacillus subtilis to obtain a final product. The EM bacteria powder can be directly scattered in a water body to inhibit pathogenic microorganisms through mass propagation of probiotics, decompose harmful substances in the water body, promote the growth of normal floras and beneficial algae in a cultivation ecosystem and keep the ecological balance of the aquaculture water body. The method is adopted to prepare the EM bacteria powder, the fermentation period is short, the process is simple, the equipment investment is low, the materials are convenient to obtain, and the method is easy to implement and is suitable for industrial production and self-production of households.

The invention discloses a method for treating mercury-containing wastewater by using modified blue-green algae. The method is characterized by comprising the following steps: (1) performing acidification modification treatment on blue-green algae, thus obtaining modified blue-green algae; (2) treating the wastewater by employing the modified blue-green algae; (3) detecting the supernatant fluid of the wastewater, and discharging the wastewater after qualification; and (4) recovering the blue-green algae, namely filtering the used modified blue-green algae, desorbing the blue-green algae in a desorbing agent for 1 hour, and filtering and refrigerating the modified blue-green algae. The modified blue-green algae raw materials used in the method come from eutrophic lakes and are easily obtained, mass propagation or dead algae in the lakes are reasonably utilized, the eutrophication phenomena can be reduced, and the treatment difficulty of the lake eutrophication is lightened; and moreover, the method for adsorbing and removing the mercury-containing industrial wastewater by utilizing the modified blue-green algae is simple in process and convenient to use.

The invention relates to the technical field of economical forest cultivation and rapid propagation and discloses a styrax hypoglauca grafting method with scion stock time separating treatment. The method includes: collecting scion before budding, sealing with wax and performing cold storage, taking stock, grafting scion, sleeving plastic bags for preserving moisture, and timely removing buds of stock when scion and stock bud to guarantee nutritional growth of scion buds. By the method, vernalization time of scion is guaranteed, budding capacity of scion is improved, stock vitality is guaranteed by delayed grafting, and withering of stock is reduced. By sleeving plastic bags for preserving moisture and removing stock buds, grafting survival rate is increased evidently, statistic average survival rate is above 67%, and mass propagation of styrax hypoglauca clone seedling is achieved.

The invention discloses an edible fruit and vegetable enzyme prepared from the following raw materials by the mass percentage: 45-80% of fruits and vegetables, 1-10% of an enzyme live keeping agent, and 15-50% of a bacterial liquid. The invention provides the edible fruit and vegetable enzyme and a preparation method thereof; preparation conditions simulate natural generation conditions of the enzyme, a principle is scientific, devices are simple, and the method is suitable for mass production; besides comprising various edible enzymes, main components of the enzyme comprise trace elements, minerals, vitamins, amino acids, proteins, carbohydrates and the like; especially, after the enzyme live keeping agent is used, the activity of the enzyme is protected, various extracellular enzymes are completive, the vitality is strong, moreover, most of beneficial living bacteria can smoothly pass through a stomach, are set in intestinal tracts and perform mass propagation, various enzymes are produced, food in the intestinal tracts are effectively decomposed, junk of the intestinal tracts is cleaned up, beneficial microbial flora of the intestinal tracts are maintained, and the intestinal tracts are facilitated to absorb various nutrients.

Mass propagation of harmful algae is harmful to the health and safety of people. Current methods to control algae bloom have respective advantages but all have rather large limitation, for example, limited effect or high cost or secondary pollution caused by the introduction of chemical substances; especially, the methods cannot solve the excessive reproduction of harmful algae on a large scale. The invention provides a simple, effective and cheap method which is convenient to solve the excessive reproduction of harmful algae on a large scale. The treatment method adopts prolific agricultural waste-straws as a material, and therefore, the source is wide, the operation is convenient and the method can be applied on a large scale in waters such as rivers, lakes, reservoirs, etc.

The invention relates to a method for artificial mass propagation of rice field eel seedlings, and belongs to the technical field of scientific application of fishery. The method is characterized by applying oral oxytocic hormone to rice field eels, namely feeding parent rice field eels with a certain dosage of oxytocic hormone by using animal bait as a carrier of the oxytocic hormone; and the method comprises the following steps of: stopping feeding of both male and female parent rice field eels for 1 day before spawning induction; observing oestrus conditions of bubbling and nesting of the male and female rice field eels in 3 to 4 days after the male and female rice field eels ingest an oxytocic hormone liquid medicament, and surrounding and marking nests with eichhornia crassipes after the nesting of the parent rice field eels is finished, wherein an inner net cage in a paddy field is used as a spawning pond; and naturally inseminating the parent rice field eels in the spawning pond, breaking membranes by fertilized ova and emerging seedlings after 144 hours, inhabiting all fish fries in the eichhornia crassipes surrounding the nests of the parent rice field eels after the fish fries break the membranes after 96 hours, and collecting the rice field eel seedlings at the time. By applying the method of the invention, the spawning induction ratio of the parent rice field eels reaches 80 to 90 percent, the hatching rate of natural spawning and fertilization is over 95 percent and the collection ratio of the rice field eel seedlings is over 95 percent.

The invention discloses a conservation and breeding method for rice photosensitive and temperature-sensitive sterile line, wherein the sterile lines are subject to twice linear light cryogenic temperature determination, and strains with low sterility initial point temperature and stable genetic homozygosity are selected for trophosome permanent conservation, and kern seeds are breeded from the expansion of the conserved trophosomes, thus realizing the mass propagation of sterility seeds for production of hybrid seeds.