34 results about "Gigantea" patented technology

The invention discloses a method for identifying a radix rosae gigantea extract, which comprises the following steps of: 1, establishing standard finger-prints of phenolic acid components, namely preparing test solution, preparing reference object solution, preparing octadecyl silica bonded silica gel serving as a stationary phase, performing gradient elution by aqueous solution of acetonitrile and glacial acetic acid, and absorbing the reference object solution and the test solution precisely and respectively, and measuring by liquid chromatography to formulate the finger-prints of the phenolic acid components in the radix rosae gigantea extract, wherein the theoretical plate number is between 5,000 and 20,000 by catechin; 2, measuring the sample finger-prints of the phenolic acid components in the radix rosae gigantea extract to be measured by a method which is the same as that used in the step one; and 3, comparing the sample finger-prints of the phenolic acid components in the radix rosae gigantea extract to be measured with the standard finger-prints of the phenolic acid components in the radix rosae gigantea extract, and identifying the quality of the radix rosae gigantea extract. The invention provides a new method and means for evaluating the quality of the radix rosae gigantea extract completely and accurately.

The present invention discloses Gymnosphaera gigantea propagating process. Gymnosphaera gigantea spore is first sterilized and then inoculated onto1 / 12-1 / 4MS solid culture medium to obtain sporophore seedling, and the sporophore seedling is transplanted to obtain Gymnosphaera gigantea. The present invention provides one way of propagating Gymnosphaera gigantea artificially. The said process makes it possible to obtain great amount of sporophore with small amount of spore, and realize the large scale industrial production of Gymnosphaera gigantea. In addition, the present invention provides excellent experiment system for relevant research and has wide application foreground.

The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising Aspergillus fumigatus (A. fumigatus) and expressing one or more genes of the ergot alkaloid biosynthesis pathway from one or more fungus selected from the group consisting of Epichloë festucae var. lolii×Epichloë typhina isolate Lp1 (E. sp. Lp1); Claviceps species; Claviceps africana (C. africana); Claviceps gigantea (C. gigantea); Epichloë coenophiala and Periglandula species, wherein gene easA or gene easM is inactivated in said A. fumigatus, is provided.

The invention discloses a sandwiched dried tofu containing silkworm chrysalises and pig intestines, and a preparation method thereof. The sandwiched dried tofu comprises the following components in parts by weight: soy bean 200-220, winged bean 12-14, tomato seed oil 9-11, salt 2-3, chili powder 2-3, Sichuan pepper powder 2-3, sesame oil 3-4, tomato juice 10-12, duck breast meal 11-14, silkworm chrysalis meal 13-16, pig intestines 13-16, beef omasum 9-12, broccoli 8-10, white gourd 8-10, raw rice sprout 7-9, Vicia gigantea 4-6, bee pollen 4-6, folium eriobotryae 5-6, Luffa cylindrical flower 3-4, Cirsium japonicum 2-3, Scrophularia ningpoensis 2-3, jasmine flower 5-6, and nutrient solution 20-30. The beef omasum, pig intestines, duck breast meal and silkworm chrysalis meal are added into the sandwiched dried tofu, the dried tofu is rich in nutrition and special in flavor, silkworm chrysalis meal has the effects of producing the body fluid, quenching thirst, promoting digestion and regulating vital energy, and the added Chinese medicines have the effects of cooling the blood and stopping bleeding, nourishing yin to reduce pathogenic fire, protecting liver, resisting microbes, and improving immunity.

The invention discloses a medicine composition for treating spleen and stomach damp-heat type peptic ulcer and a method for preparing the medicine composition. The medicine composition comprises diaphragma juglandis, Cipadessa cinerasce-ns (Pell.) Hand.-Mazz., radix glycyrrhizae, asian butteflybush roots and leaves, filipendula palmata, violet, codonopsis pilosula, smilax stans, hedyotis diffusa, herb of largeserrate molsa, fructus chaenomelis lagenariae, clerodenrum bungei steud, polygonum hydropiper, textile screw pine shoot, michelia alba, pararuellia delavayana, cirsium vlassovianum, camellia oil, biondia henryi, fructus psoraleae, cynanchum glaucescens, loofah juice, delavay falsepanax herb, blumea, caulis clematidis armandii, panax notoginseng, radix fici simplicissimae, corydalis gigantea Trautv. & C.A. Mey. and hypericum attenuatum. The medicine composition and the method have the advantages that effects of regulating spleens, harmonizing stomachs, clearing heat, removing dampness, promoting qi circulation and strengthening the spleens can be realized by the medicine composition, the medicine composition is mainly used for treating the spleen and stomach damp-heat type peptic ulcer, the stomach mucosae can be protected, the damp-heat constitution of patients further can be effectively improved, immune functions of the bodies of the patients can be integrally regulated, the medicine composition can become effective fast and is high in efficiency, short in treatment course, free of toxic and side effects and low in cost, definite curative effects can be realized, and the like.

The present invention provides a method of producing lysergic acid and other ergot alkaloids by genetic modification of a fungus. A strain of fungus comprising Aspergillus fumigatus (A. fumigatus) and expressing one or more genes of the ergot alkaloid biosynthesis pathway from one or more fungus selected from the group consisting of Epichloë festucae var. lolii×Epichloë typhina isolate Lp1 (E. sp. Lp1); Claviceps species; Claviceps africana (C. africana); Claviceps gigantea (C. gigantea); Epichloë coenophiala and Periglandula species, wherein gene easA or gene easM is inactivated in said A. fumigatus, is provided.

A process for extracting the antibacterial substance from the mucilage of S. gigantea includes such steps as thawing said mucilage, proportionally adding it to the extracting solvent prepared from water, Na4EDTA, phenylmethyl sulfuryl fluoride, aprotinin and acetic acid, stirring on ice bath to obtain liquid extract, centrifugal of supernatant, filtering, ultrafiltering, concentrating, and freeze drying.

The invention relates to a belt for treating dysmenorrhea and a preparation method thereof. The medicine packed in the belt is prepared from small galangal, valeriana jatamansi Jones, fructus galangae, tsinling larkspur root, tree-of-heaven ailanthus bark, Pinellia integrifolia N. E. Brown, Murraya exotica, Rabdosia serra (Maxim.) Hara, Eupatorium japonicum Thunb, Cacalia ainsliaefora(Franch.) Hand.-Mazz., Lysimachia decurrens Forst, Chinese trumpet creeper, Alsophila gigantea, Dichrocephala auriculata (Thunb.) Druce, Lysimachia foenum-graecum Hance, Triumfetta pilosa Roth, Marsdenia longipes W. T. Wang, Artemisia anomala S. Moore, Helicteres isora L., laminated sanicle herb, Damnacanthus macrophyllus Sieb. ex Mig. var giganteus ( Makion ) koidz, Ventilago leiocarpa Benth, and Abelmoschus sagittifolius(Kurz)Merr. The belt of the invention can sooth the liver for smooth circulartion of Qi, promoting blood circulation to remove blood stasis, soften hard lumps and dispel nodes, and eliminate cold to stop pain. Moreover, the belt of the invention has the advantages of simple and feasible preparation process, and remarkable clinical effect on dysmenorrhea patients.

The invention discloses a method for increasing the content of secondary metabolites in the black rice tree by infecting with Megacystis maxima. The present invention adopts Megacystis maxima to infect the root system of the black rice tree to form a symbiosis system of the black fan tree and the giant giant cyst G. gigantea. Through the mutual influence and mutual benefit and mutual assistance relationship between G. gigantea and the soil of the root system of the black rice plant, the content of secondary metabolites in the black rice plant is significantly increased. The invention can remarkably increase the content of secondary metabolites in the plant of the black rice plant, wherein the content of flavonoids is increased by no less than 0.01%, the content of iridoid glycosides is increased by no less than 0.01%, and the content of proanthocyanidins is increased by no less than 0.01%. At 0.01%, the content of other secondary metabolites increases by not less than 0.01%. The present invention can increase the content of iridoid glycosides, the functional components of black rice tree branches and leaves, and improve its coloring ability; it can also increase the content of flavonoids and proanthocyanidins secondary metabolites, thereby increasing the nutritional content of black rice .

The invention relates to a traditional Chinese medicine for treating sciatica. The traditional Chinese medicine is prepared from the following medicinal materials by weight: 13 parts of scorzonera divaricata, 15 parts of pseudobulbus calanthes fimbriatae, 12 parts of gymnosphaera gigantea leaf, 12 parts of sedum aizoon l. var. floribundum nakai, 14 parts of sarcanthus elongatus rolfe, 16 parts of lloydia thibetica baker, 12 parts of vernonia gratiosa, 12 parts of phyla nodiflora, 15 parts of rhizome of david anemone, 12 parts of cyclosorus aridus, 14 parts of bottle gourd peel, 14 parts of pellionia radicans, 14 parts of diphasiastrum complanatum, 12 parts of acanthopanax senticosus, 14 parts of aralia undulata, 16 parts of linaria buriatica, and 8 parts of amanitopsis volvata sacc. Clinic experiments prove that the traditional Chinese medicine can safely and effectively treat sciatica.

The invention discloses application of broccoli to inhibiting of weed germination and growth, and belongs to the technical field of weeding. The seed germination inhibition rate of the broccoli to weed of Chenopodium album, Artemisia hedinii, Setaria viridis (L.) Beauv. var. gigantea, Chloris virgata and Panicum miliaceum can reach 98%, 100%, 98%, 97%, 98%. Through a potting effect control test, the result shows that the weed germination inhibition rate of the broccoli reaches 94.5%. In a field test, the broccoli can significantly inhibit germination and growth of the Chenopodium album. The broccoli is applied to the technical field of weeding, the advantage of high efficiency, safety, environmental protection and the like are achieved, and development and application prospects are wide.

The invention discloses application of broccoli to inhibiting of weed germination and growth, and belongs to the technical field of weeding. The seed germination inhibition rate of the broccoli to weed of Chenopodium album, Artemisia hedinii, Setaria viridis (L.) Beauv. var. gigantea, Chloris virgata and Panicum miliaceum can reach 98%, 100%, 98%, 97%, 98%. Through a potting effect control test, the result shows that the weed germination inhibition rate of the broccoli reaches 94.5%. In a field test, the broccoli can significantly inhibit germination and growth of the Chenopodium album. The broccoli is applied to the technical field of weeding, the advantage of high efficiency, safety, environmental protection and the like are achieved, and development and application prospects are wide.

The invention provides an application of a rose odorata var gigantea fruit extract in preparation of medicine, health care product or food for treating and / or preventing stomach diseases. Experiments prove that the rose odorata var gigantea fruit extract has the effects of treating atrophic gastritis and repairing gastric mucosal lesion, and the curative effect is exact. The invention discloses a new application of the medicinal material rose odorata var gigantea fruit.

The invention discloses a quantitative PCR (Polymerase Chain Reaction) primer for a soybean E2 / GIGANTEA homologous gene and an application of the quantitative PCR primer. The invention provides a setof specific quantitative PCR primers for detecting the expression quantity of the soybean E2 / GIGANTEA homologous gene. The specific quantitative PCR primers are six single-stranded DNA shown as sequences 1-6 in a sequence table. By adopting a real-time quantitative PCR method, the expression characteristics of GIGANTEA (GI) and homologous genes thereof are defined, and the gene not only plays an important role in the soybean growth period, but also plays an important role in circadian movement / circadian clock, so that the gene expression is detected to play an important role in soybean biological research and application. The primer disclosed by the invention has great significance for understanding and mastering the growth habits of soybeans.

The invention discloses a pharmaceutical composition for treating tinea pedis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials: grenateleaf heteropappus herb, pennycress, Cydonia oblonga, Adenophora trachelioides, Japanese saussurea herb, rhynchosia volubilis, nakedflower beautyberry branchlet and leaf, honeysuckle, Vitis bryoniifolia Bunge, apricot kernel, gambleana spicebush root and leaf, malabarnut branchlet and leaf, nephelium lappaceum, patience dock root, Syzygium sanarangense, rhizome of Largeleaf Japanese Ginseng, duckweed, ashycoloured ironweed herb, Sanguisorba officinalis, polygonatum, Gorgon fruit, cape jasmine, common embelia root branchlet and leaf, Corydalis gigantea, henry sarmentose fig receptacle, cladonia fallax lichen and robust leontice rhizome and root. The invention has the beneficial effects that the pharmaceutical composition has the efficacies of clearing heat, detoxifying, relieving swelling and pain, dispelling wind and dampness and relieving itching; and the pharmaceutical composition has the advantages of quick and high effectiveness, high efficiency, definite therapeutic effect, short treatment course, no toxic or side effect, and low price.

The invention belongs to the field of traditional Chinese medicine preparations, and specifically provides a honey health care product, the active ingredient contained in which is prepared by extracting the following raw materials by weight: 2 parts of honey, 20 parts of forsythia forsythia, 25 parts of slippery leaf dieda, 18 parts of Huanghuaren, 20 parts of Kalanchoe, 15 parts of Hongmuxiang, 18 parts of Esperia galerica, 12 parts of Salvia miltiorrhiza, 5 parts of licorice, and then adopt conventional production technology to make soft capsules. The invention can nourish qi and blood.

The invention relates to an OsAGP13 (Oryza sativa Annotation Gigantea Project 13) gene for controlling rice flowering and application of an RNA (Ribonucleic Acid) interference fragment of the OsAGP13 gene, belonging to the field of plant genetic engineering; the nucleotide sequence of the RNA interference fragment of the OsAGP13 gene is represented by SEQIDNO:1; an OsAGP13-RNAi carrier is constructed by utilizing a genetic engineering technology; an OsAGP13-RNAi transgenic plant having an advanced heading and flowering period is obtained through a transgenic technology; the invention also proves that expression of an OsFTL1 gene is inhibited by the OsAGP13 gene; in the OsAGP13-RNAi plant, due to inhibition of expression of the OsAGP13 gene, high expression of the OsFTL1 gene is caused, so that rice is promoted to flower in advance; and the result shows that rice flowering can be controlled by the OsAGP13 gene. The OsAGP13 gene and the interference fragment of the OsAGP13 gene disclosed by the invention can be used for researching molecular biological mechanisms influencing rice flowering and controlling rice flowering.

The invention relates to a traditional Chinese medicine for treating polyarteritis nodosa. The traditional Chinese medicine is prepared from medicine materials by weight: 12 parts of oleanderleaf pittosporum fruit or herb, 3 parts of passiflora papilio, 15 parts of meconopsis georgei tayl, 15 parts of amur honeysuckle, 3 parts of denseleaf crazyweed herb, 10 parts of narrowleaf rockvine root or herb, 15 parts of marrow honey-suckle, 8 parts of geastrum triplex, 9 parts of dianella ensifolia, 12 parts of Chinese potentilla herb, 10 parts of gymnospermium kiangnanense, 14 parts of corydalis gigantea, and 10 parts of common calanthe herb. Clinical experiments show that the traditional Chinese medicine can effectively and safely treat the polyarteritis nodosa.

The invention provides a fertilized egg hatching system for schizothorax gigantea. The fertilized egg hatching system for schizothorax gigantea comprises a water accumulation pool and a plurality of hatching sets arranged in the water accumulation pool; the incubation set comprises a collecting tank and production supports arranged on the two sides of the collecting tank, and a plurality of incubators are arranged on the production supports side by side. A transverse water pipe is arranged above the production support, water outlet pipes with valves corresponding to the incubators are arranged on the transverse water pipe, and each water outlet pipe is connected with a water inlet hose; water outlets are formed in the water accumulation pool and the collecting tank; and the collecting tank is provided with a screen in front of the water outlet. The hatching system is high in hatching roe survival rate, high in production efficiency and capable of achieving large-scale production in Tibet.

The invention relates to the technical field of agricultural breeding, and in particular relates to ointment for treating anal prolapse of poultry. The ointment is prepared from the following raw materials in parts by weight: 20-30 parts of radix astragali, 6-15 parts of white atractylodes rhizome, 3-6 parts of tuber of Colocacia gigantea Hook.f, 30-40 parts of pericarpium granati, 3-8 parts of pericarpium citri reticulatae, 8-10 parts of galla chinensis, 4-7 parts of radix bupleuri, 6-13 parts of fructus aurantii, 15-30 parts of earthworm extracting solution, and 2-8 parts of alum. For the ointment, the synergistic treatment of the traditional Chinese medicinal materials is adopted, the ointment has the advantages of being free of side effects, being environmentally friendly, being low incost, and being good in effect, the parturient canal injuries of the poultry can be rapidly repaired, the occurrence of anal prolapse of the poultry can be prevented, and the morbidity of anal prolapse and other egg-laying injury diseases can be reduced.

A process for extracting the antibacterial substance from the mucilage of S. gigantea includes such steps as thawing said mucilage, proportionally adding it to the extracting solvent prepared from water, Na4EDTA, phenylmethyl sulfuryl fluoride, aprotinin and acetic acid, stirring on ice bath to obtain liquid extract, centrifugal of supernatant, filtering, ultrafiltering, concentrating, and freeze drying.

The invention relates to a vicia gigantea spore sowing substrate capable of preventing damage caused by weeds and pests. The vicia gigantea spore sowing substrate is prepared by taking the following raw materials: 100-120 parts of leaf mould, 20-25 parts of Kanuma volcanic ash soil , 30-38 parts of fermented saw dust, 1-1.5 parts of black alum, 0.3-0.5 part of photosynthetic bacteria, 1-2 parts of vitamin B6, 5-8 parts of calcium magnesium phosphate fertilizer, 2.3-2.5 parts of naphthylacetic acid, 10-12 parts of amino acid fertilizer, and 0.03-0.05 part of p-chlorophenoxyacetic acid. The substrate is rich in nutrition, and loose and ventilating in texture, and has certain water retention capacity; the fertility can be easily absorbed and utilized, the weed growth is inhibited, damage caused by weeds and pests is reduced, the germination ratio of vicia gigantea spores cannot be influenced, and further, the germination time that vicia gigantea spores propagate into prothallia can be reduced.

The invention discloses a method for increasing a content of secondary metabolites in vaccinium bracteatum by means of G. gigantea infection. According to the method for increasing the content of thesecondary metabolites in vaccinium bracteatum by means of G. gigantea infection, a root system of vaccinium bracteatum is infected with G. gigantea to form a symbiotic system of vaccinium bracteatum and G. gigantea. Through mutual effects and a mutual beneficial relation which are formed between G. gigantea and soil around the root system of vaccinium bracteatum, an significant increase of the content of the secondary metabolites in vaccinium bracteatum plants is promoted. By means of the method for increasing the content of the secondary metabolites in vaccinium bracteatum by means of G. gigantea infection, the content of the secondary metabolites in the vaccinium bracteatum plants can be significantly increased, wherein the content of flavone compounds is increased by 0.01% or above, thecontent of iridoid glycoside components is increased by 0.01% or above, the content of procyanidin components is increased by 0.01% or above, and the content of other secondary metabolites is increased by 0.01% or above. By means of the method for increasing the content of the secondary metabolites of vaccinium bracteatum by means of G. gigantea infection, the content of the efficacy components,namely the iridoid glycoside components, for dyeing branches and leaves of vaccinium bracteatum can be increased, so that dyeing power of the branches and leaves of vaccinium bracteatum is improved; and the content of the flavone secondary metabolites and the content of the procyanidin secondary metabolites can be increased, so that nutritional components of black rice are increased.

The invention relates to the technical field of prevention and treatment of Eulecanium gigantea Shinji diseases, in particular to an organic foliar fertilizer for preventing and treating Eulecanium gigantea Shinji diseases and a preparation method thereof. The organic foliar fertilizer is prepared by mixing pyroligneous liquor serving as a main raw material, nano silicon oxide and nano titanium oxide serving as inorganic auxiliaries and crushed chili seeds; and the organic foliar fertilizer is diluted to 200-500 times and sprayed to the lesion of the trunk to realize the prevention and treatment of the Eulecanium gigantea Shinji diseases. Any artificially synthesized chemical fertilizer and pesticide do not need to be added, and by means of effective components in the pyroligneous liquor and the combined action of inorganic and organic harmless components, the growth environment of the Eulecanium gigantea Shinji eggs is effectively destroyed and the coccidiococcus jujube eggs die whilefertilization is conducted.

A method of preparation of a potential herbal anti-cancer medicine from the leaves and twigs of Calotropis gigantean for prevention and treatment of all types of cancers, using fresh extract of leaves and twigs of Calotropis gigantea fortified with unrefined sugarcane sugar (jaggery). The aqueous extract of leaves and twigs of Calotropis gigantea was desiccated. The [3 H]-Thymidine growth assay was performed against cancer cell lines, WM 902-B and WM1341-D. The extract [28 mgms] was dissolved in 2 ml control at 0.5%, 0.1% DMSO. The extract is highly inhibitory at all dilutions and highly inhibitory to both the cancer cell lines.

The invention provides a method for collecting mucous from giant S.gigantea, which comprises the following steps: (1) prying out a block of healthy giant S. gigantean of moderate size from seabed; (2) knocking the top of pried out coral with extra force for more than twice; (3) immersing in sea for 50-100 minutes; (4) taking out the coral and putting into collecting basin to collect the mucous; (5) pouring the mucous into plastic centrifuge tube, maintaining under temperature of minus 20 Deg. C. The invention collects the mucous from giant S. gigantean by means of physical stimulation, which avoids great damage to coral and guarantee the survival of coral after returning to sea, and the nonuse of chemical agent makes little influence to further research.

The invention discloses an analgesic plaster for treating chronic purely traumatic lumbocrural pain and a preparation method for the analgesic plaster. The analgesic plaster for treating chronic purely traumatic lumbocrural pain is mainly prepared from the following raw medicaments in parts by weight: 40 to 82 parts of docynia fruits, 40 to 70 parts of hymenocallis littoralis, 35 to 70 parts of cuneate lespedeza, 35 to 70 parts of pedilanthus, 33 to 65 parts of zanthoxylum schinifollum, 30 to 60 parts of elaeagnus umbellata, 30 to 50 parts of wightia speciosissima, 30 to 45 parts of corydalis gigantea trautv. & C. A. Mey., 30 to 40 parts of radix podophylli, 28 to 40 parts of simao premna, 25 to 35 parts of excoecaria cochinchinensis, 22 to 30 parts of cyathea gigantea, 20 to 30 parts of phlomis mongolica, 18 to 30 parts of root and rhizome of smallflower iris and 15 to 30 parts of eriosema himalaicum ohashi. According to the analgesic plaster and the preparation method, multiple traditional Chinese herbal medicaments are reasonably selected for combination according to the theory of traditional Chinese medicine, so that the effects of relaxing muscles and tendons, promoting blood circulation, dredging collaterals, removing blood stasis, alleviating pain, promoting qi circulation and diminishing swellings can be achieved, and a special curative effect on chronic purely traumatic lumbocrural pain is achieved; the prepared plaster is externally applied, active pharmaceutical ingredients directly reach lesions, the efficacy is enhanced, and side effects on a human body can also be reduced.

The invention provides a nutritional agent for increasing a peanut oil yield and a preparation method of the nutritional agent. The nutritional agent comprises the following raw materials: 80-95 partsof hypolepis gigantea leaves, 150-170 parts of oxytropis assiensis vass petal, 160-180 parts of saussurea stricta leaves and 300 parts of water. After a series of treatment by the nutritional agent,soaked peanuts are sowed, diameters of the peanuts are effectively increased, and the plump degrees of the peanuts are raised greatly, so that the output and oil yield of the peanuts are greatly increased.