15 results about "Capsaicinoid" patented technology

This invention discloses a method for preparing capsaicin-like substances by temperature-responsive eutectic solvent extraction, comprising the following steps: drying Yunnan chili peppers (after removing stems and seeds), pulverizing, sieving, and collecting the chili powder; mixing lidocaine (HBA) with six alkane-chain carboxylic acids (valerate, hexanoic acid, heptanoic acid, octanoic acid, nonanoic acid, and lauric acid) at a 1:1 molar ratio to form a homogeneous and transparent liquid, TRDES; weighing the chili powder, adding it to the TRDES aqueous solution, and extracting by shaking; after extraction, centrifuging, and diluting the supernatant to obtain capsaicin-like substances. This invention uses temperature-responsive eutectic solvent extraction to prepare capsaicin-like substances, which is simple, highly efficient, and allows for the recycling of extraction reagents, effectively saving resources. It has significant practical and industrial value for promoting the large-scale production of capsaicin-like substances and enhancing their high-value applications.

The invention discloses a capsicum CaVAP27 gene and application of a capsicum CaVAP27 protein in regulation and control of capsaicin content. A CDS sequence of the capsicum CaVAP27 gene is shown as SEQ ID NO: 1, and an amino acid sequence of the capsicum CaVAP27 gene is shown as SEQ ID NO: 2. It is found that the expression level of the VAMP related protein gene CaVAP27 is remarkably related to the blister number and the capsaicin content of pepper fruits, the pepper CaVAP27 gene may influence the accumulation level of capsaicin by influencing blister formation, and discovery of the pepper CaVAP27 gene function provides a theoretical basis for creation of pepper varieties for regulating and controlling the capsaicin substance content.

The invention discloses a capsicum CaABCB9 gene, a capsicum CaABCB9 protein and application of the capsicum CaABCB9 gene and the capsicum CaABCB9 protein in regulation and control of capsaicin content. The CDS sequence of the capsicum CaABCB9 gene is shown as SEQ ID NO: 1, and the amino acid sequence is shown as SEQ ID NO: 2. It is found that the capsicum CaABCB9 gene is highly expressed in capsicum tissue with the highest capsicine content, the silent capsicum CaABCB9 gene can inhibit the capsicine from being transferred into diaphragm epidermis vesicles, synthesis inhibition of the capsicine is further fed back, the capsicine can be ingested into cells through heterologous expression of the capsicum CaABCB9 gene, and therefore the capsicum CaABCB9 gene can be used for preparing the capsicum tissue with the highest capsicine content. The capsicum CaABCB9 gene provides a new target for regulating the accumulation level of capsaicin substances and improving the quality and breeding efficiency of capsicum.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a CcMYB1R1 protein and a gene in regulating and controlling the content of capsaicin substances of capsicum. The invention provides the CcMYB1R1 protein for regulating and controlling the content of capsaicin substances, and the CcMYB1R1 protein is a key transcription factor for regulating and controlling synthesis of capsaicin substances in capsicum fruits, can promote biosynthesis of capsaicin substances, and has an important regulating and controlling effect on quality formation of capsicum fruits. By regulating and controlling the content of the CcMYB1R1 protein or the expression quantity of the CcMYB1R1 gene, the content of capsaicin substances in the pepper fruits can be effectively regulated and controlled, a new key gene resource is provided for analyzing a secondary metabolism regulation and control network of the pepper fruits, and a direct genetic target and an important genetic material are provided for cultivating new germplasm of the pepper.

The application belongs to the technical field of food analysis, and discloses a comprehensive quality evaluation method of hot and spicy seasoning. The application firstly organically fuses the absolute content of key flavor substances determined by a GC-MS internal standard method, the total content of capsaicin substances determined by HPLC, and the total content of amides determined by ultraviolet spectrophotometry, constructs a comprehensive quality index (CQI), overcomes the defects of one-sidedness of single index evaluation, can scientifically distinguish the comprehensive quality differences of non-balanced samples such as "high spiciness and low fragrance" and "high numbness and low spiciness", and the evaluation result has high correlation with the comprehensive satisfaction of experts' senses.

The invention relates to the technical field of plant genetic breeding, in particular to a directional pyramiding breeding method for improving the pungency degree and color value of processed chilies. Comprising the steps of parent screening (molecular marker identification of high-pungency and high-color-value germplasm based on capsaicin synthesis key genes Pun1 and Capana06g001204 and capsorubin regulatory genes CaBBX20 and CCS), hybridization polymerization (multi-generation backcross and molecular marker-assisted selection integration of pungency degree / color value genes), ploidy breeding accelerated homozygosis (a microspore culture combined chromosome doubling technology), and cross breeding of the high-pungency and high-color-value germplasm. And performing phenotype verification (detecting the content of capsaicin substances by high performance liquid chromatography and determining the color value by spectrophotometry). According to the method, gene modules for controlling the pungency degree and the color value are directionally polymerized, a special dry processing variety with the capsaicin content being larger than or equal to 3% (the corresponding pungency degree being larger than or equal to 30,000 SHU) and the color value being larger than or equal to 300 ASTA is bred, the breeding period is shortened to 1 / 3 of that of a traditional method, and the requirement of the pepper processing industry for high-quality raw materials is met. The method is suitable for large-scale pepper breeding, and is particularly suitable for planting in high-altitude and arid regions.

The invention relates to the technical field of agricultural biology, in particular to a specific primer of a molecular marker InDel.qCC2.1. 150 linked with the content of capsaicin substances and application of the specific primer. A segment of base insertion remarkably related to the capsaicin content exists at the position of 150438157bp of a chromosome 2 in a PI152225 material, the base insertion is close to a major qCC2.1 and is closely linked with a gene Cchi02g002094, an InDel molecular marker is designed, and a specific primer is designed. The primer can be used for screening hot peppers with different capsaicin substance contents in the seedling stage of the hot peppers, so that the capsaicin substance content determination work is relieved, the screening of materials with different pungency degrees is accelerated, and the primer has a very important effect on molecular genetic breeding of the hot peppers with different pungency degrees. As the marker is very close to the gene Cchi02g002094, the identification rate of the gene is very high.

Topical polyherbal oleogel composition, comprising, in weight percent of the finished product: (i) Boswellia serrata CO2 select extract 1.0–5.0%; (ii) Curcuma longa phytosome 0.5–2.5% with curcuminoids complexed to phospholipids together with turmerone-rich turmeric essential oil 0.2–0.8%; (iii) Zingiber officinale CO2 select extract 0.5–2.5%; (iv) Hypericum perforatum macerate oil 3.0–10.0%; (v) Capsaicinoids in an equivalent amount of 0.01–0.05%, obtained from Capsicum oleoresin, and Mentha piperita oil 0.3–1.0% together with Rosmarinus officinalis oil 0.2–0.6%; (vi) a carrier phase of sesame oil and medium-chain triglycerides in a ratio of 2:1 to 1:1, and castor oil 8–12% and squalane 1.0–3.0%; (vii) a structuring phase of sunflower lecithin 0.8–2.0% and beeswax 3.0–6.0%, providing a lamellar, shear-thinning oleogel; and (viii) mixed tocopherols 0.1–0.3% and a rosemary diterpene antioxidant extract 0.05–0.2%;wherein the composition is anhydrous with a water activity ≤ 0.55, and wherein the mass ratio of total boswellic acids to total curcuminoids is 0.8:1 to 1.6:1 and the mass ratio of menthol to total capsaicinoids is 10:1 to 30:1.;

The invention relates to the technical field of agricultural biology, in particular to a qCC2. 1. InDel.151 specific primer pair closely linked with the content of capsaicin substances and application of the qCC2. 1. InDel.151 specific primer pair. According to the application, a base which is remarkably related to the capsaicin content is inserted at the position of 151083393bp of a chromosome 2 in a low-pungency material PI152225, when a pungency value (SHU) 100000 is taken as a threshold value, except for heterozygotes, 91.94% of the base at the site is CTTTCATTGTGTTTTTCAG, and except for the heterozygotes, 74.54% of the base at the site is C in the material with a pungency value greater than 100000. The marker is very close to the gene Cchi02g002094, and the identification rate of the gene is very high, so that the marker has important significance on molecular marker-assisted selective breeding.

The invention provides a primer for identifying the content of capsaicin substances in pepper and application of the primer, and belongs to the technical field of gene engineering. The nucleotide sequence of a forward primer of the primer for identifying the content of capsaicin substances in hot peppers is shown as SEQ ID NO.1, and the nucleotide sequence of a reverse primer of the primer is shown as SEQ ID NO.2. The primer is used for detecting whether the insertion / deletion fragment exists in the upstream of the coding region of the pepper AT3 gene or not, so that the content of the capsaicin substances in the pepper can be judged. According to the method, the content of capsaicin substances in the hot peppers can be identified in the seedling stage of the hot peppers, a new method can be provided for cultivation of the high-capsaicin variety hot peppers, the breeding and genetic improvement cycle of the high-capsaicin variety hot peppers can be shortened, and the method has wide application prospects.

The invention relates to the technical field of agricultural biology, in particular to a specific dCAPS primer pair of SNP closely linked with the content of capsaicin substances and application of the specific dCAPS primer pair. According to the application, PBC932 is taken as a reference genome, a single base mutation exists at 150448409bp of a chromosome 2 in a PI152225 plant material, the mutation is in a gene Cchi02g002095 (Pun1), a molecular marker is designed in the gene Cchi02g002095 (Pun1), the molecular marker is named as a marker dCAPPSPh1, and a primer is designed. The primer can be used for screening hot peppers with different capsaicin substance contents in the seedling stage of the hot peppers, so that the capsaicin substance content determination work is relieved, the screening of materials with different pungency degrees is accelerated, and the primer plays a very important role in molecular genetic breeding of the hot peppers with different pungency degrees.

The present invention relates to a time-resolved fluorescence kit for evaluating the safety of edible vegetable oils, a preparation method, and its application. The kit comprises a fluorescent test strip and a sample reaction bottle containing a europium-labeled anti-benzo[a]pyrene monoclonal antibody, a europium-labeled anti-aflatoxin B1 monoclonal antibody, and a europium-labeled anti-capsaicinoid universal monoclonal antibody. One side of the fluorescent test strip comprises, from top to bottom, an absorbent pad, a test pad, and a sample pad, with adjacent pads overlapping and connecting at the junction. The test pad is based on a nitrocellulose membrane, which is provided with a transverse quality control line and a test line. The quality control line is coated with a rabbit anti-mouse polyclonal antibody. The test lines are located below the quality control line and are spaced apart. There are two or three test lines, each coated with a test substance-protein conjugate. The kit can achieve simultaneous and rapid detection of multiple targets on a single test strip. The kit is used for evaluating the quality and safety of edible vegetable oils, identifying waste cooking oils, and discovering strong carcinogens in edible vegetable oils.

The present invention relates to a modified release capsaicinoids formulation comprising of active core, at least one emulsifier and at least one gastro-resistant pH dependent polymer. The active core may be coated with at least one release modifying agent and optionally the outermost coating. The invention as described herein also relates to the solvent-free process, wherein emulsion comprising capsaicinoids is entrapped in at least one gastroresistant pH dependent polymer and deposited on non-pareil seeds to get active core, which may be further coated using at least one release modifying agent to get the beadlets. The formulation releases not more than 10% by weight of capsaicinoids in stomach within 2 hours, followed by release of not less than 75% of capsaicinoids in the intestine within 1 hour. The modified release formulation does not exhibit the alcohol dose dumping effect in stomach and it is safe for oral administration to the consumers.

A thermogenic composition prevents the adverse effects of cold exposure and / or mitigates hypothermia. Epinephrine, atropine sulfate, phenylephrine hydrochloride, L-carnitine, vitamin B12, magnesium sulfate, and saline solution are homogeneously mixed to form an injectable formulation for the thermogenic composition. Capsaicinoids, capsinoids, ginger extract, L-citrulline, grains of paradise extract, taurine, caffeine anhydrous, L-theanine, green tea extract, black pepper extract, potassium citrate, sodium citrate, and magnesium citrate anhydrous are heterogeneously mixed to form a capsulated formulation for the thermogenic composition.

The present invention discloses a universal monoclonal antibody HOPE1 against natural capsaicin, dihydrocapsaicin, and synthetic capsaicin, and its application. The heavy chain variable region sequence of the antibody is shown in SEQ ID NO. 1, and the light chain variable region sequence is shown in SEQ ID NO. 2. The monoclonal antibody HOPE1 has a half-inhibitory concentration of 0.32 ng / mL for natural capsaicin, 0.07 ng / mL for synthetic capsaicin, 0.21 ng / mL for dihydrocapsaicin, and a mixture of natural capsaicin, synthetic capsaicin, and dihydrocapsaicin, respectively, and has a strong affinity for capsaicinoids. The IC-ELISA immunoassay method and time-resolved fluorescence immunochromatography assay for detecting the content of capsaicinoids established using the monoclonal antibody HOPE1 provided by the present invention have the advantages of high sensitivity and low detection limit.