Cultivation method for promoting pepper growth and capsaicin synthesis

Abstract

The present invention provides a cultivation method for promoting pepper growth and capsaicinoid production, comprising the following steps: inoculating peppers with arbuscular mycorrhizal fungi during seedling cultivation, inoculating the peppers with a suspension of Priesteria gigantea 7-10 days after transplanting the pepper seedlings, spraying the peppers with a foliar fertilizer containing the Priesteria gigantea suspension at the initial stage of fruit setting, and finally spraying the peppers with abscisic acid 10-15 days before harvesting. This method, based on the nutrient requirements of peppers during different growth stages, selectively applies fertilizer types and dosages at different times, effectively promoting pepper seedling growth and increasing the capsaicinoid content of pepper fruits. The method has a short production cycle and is simple to operate.

Description

Technical Field

[0001] The invention relates to the field of crop cultivation methods, in particular to a cultivation method for promoting pepper growth and capsaicin synthesis. Background Art

[0002] Chili peppers are an important vegetable in our daily diet, and capsaicinoids are the most important quality indicator of chili peppers. There are 19 homologues, of which capsaicinoids account for 69% and are the primary determinant of their spiciness. Capsaicinoids are naturally occurring, low in toxicity, non-polluting, and possess diverse biological functions. They are currently widely used in food additives, pharmaceuticals, and biocontrol applications both domestically and internationally. Therefore, increasing the capsaicinoid content of chili peppers is crucial for enhancing their economic value. Currently, the main approach to increasing capsaicinoid content in chili peppers is through selective breeding, a method that is time-consuming, challenging, and expensive. Summary of the Invention

[0003] In light of this, the present invention provides a cultivation method for promoting pepper growth and capsaicinoid production. This method involves inoculating peppers with arbuscular mycorrhizal fungi during the seedling stage and then inoculating with Priesteria gigantea 7-10 days after transplanting, effectively promoting pepper seedling growth. Furthermore, during the fruiting stage, the present invention sprays a foliar fertilizer formulated with Priesteria gigantea bacterial solution and salicylic acid, effectively increasing the capsaicinoid content of pepper fruits. This method tailors the nutrient requirements of peppers at different growth stages to target specific fertilizers, resulting in a short cycle and simple operation.

[0004] The technical solution of the present invention is achieved as follows:

[0005] A cultivation method for promoting pepper growth and capsaicin synthesis comprises the following steps: inoculating arbuscular mycorrhizal fungi during pepper seedling cultivation, inoculating Priesteria gigantea suspension 7-10 days after the pepper seedlings are transplanted, spraying foliar fertilizer containing the Priesteria gigantea suspension on the leaves of the peppers at the early stage of fruit setting, and finally spraying abscisic acid on the leaves 10-15 days before the peppers are harvested.

[0006] Furthermore, the arbuscular mycorrhizal fungi are one or two of Funneliformis mosseae, Glomus intraradices and Glomus etunicatum; and the inoculation amount of the arbuscular mycorrhizal fungi is 10%-20% of the mass of the seedling medium during pepper seedling cultivation.

[0007] Furthermore, the effective viable bacteria count of the arbuscular mycorrhizal fungi is 1-4×10 8 cfu / mL.

[0008] Furthermore, the effective viable bacteria count of the arbuscular mycorrhizal fungi is 4×10 8 cfu / mL.

[0009] Furthermore, pepper seedlings are transplanted when they have 4-6 true leaves.

[0010] Furthermore, the inoculation volume of the Priesteria gigantea suspension is 1-3 ml / strain, and the effective viable count is 2-4×10 8 cfu / mL.

[0011] Furthermore, the preparation process of the Priesteria gigantea suspension is as follows: under sterile conditions, the Priesteria gigantea strain is inoculated onto a solid culture medium, cultured at a constant temperature of 28-30°C, and then a single colony is picked and inoculated into a liquid culture medium, cultured at 28-30°C and 180-200 r / min for at least 24 hours, and examined under a microscope until the effective viable count is 2-4×10 8 cfu / mL, and the suspension of Priesteria gigantea was obtained.

[0012] Furthermore, the foliar fertilizer preparation process containing the suspension of Priesteria gigantea is as follows: a single colony of the suspension of Priesteria gigantea is picked and inoculated into a liquid culture medium, and after shaking culture for 6-8 hours, 4-8 g of sodium phosphate and 20-30 g of salicylic acid are added to each liter of the suspension in sequence, and then the shaking culture is continued for 20-24 hours, and the suspension is examined under a microscope until the effective viable count reaches 6-10×10 8 cfu / mL, and finally adding 0.05-0.1% of the total mass of sodium benzoate to obtain a foliar fertilizer containing Priesteria gigantea suspension.

[0013] Furthermore, the foliar fertilizer is sprayed once every 7-10 days, with a dilution ratio of 200-300 times, sprayed 2-3 times, and an application amount of 30-50 L / mu.

[0014] Furthermore, the spraying concentration of the abscisic acid is 15-25 mg / L, and the application amount is 30-50 L / mu.

[0015] Furthermore, the solid culture medium is LB solid culture medium; and the liquid culture medium is LB liquid culture medium.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] The invention applies corresponding fertilizer types and amounts in different periods in a targeted manner according to the nutrient demand characteristics of peppers in different growth stages, which can effectively promote the growth of pepper seedlings and also increase the capsaicin content of pepper fruits. The cycle is short and the operation is simple.

[0018] The invention can effectively promote the growth of pepper seedlings and significantly increase the capsaicin content of pepper fruits through the synergistic effect of arbuscular mycorrhizal fungi, Priesteria gigantea and foliar fertilizer prepared from Priesteria gigantea and salicylic acid. DETAILED DESCRIPTION

[0019] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention.

[0020] Unless otherwise specified, the experimental methods used in the examples of the present invention are all conventional methods.

[0021] Unless otherwise specified, the materials, reagents, etc. used in the examples of the present invention can be obtained from commercial sources.

[0022] Example 1

[0023] A cultivation method for promoting pepper growth and capsaicin synthesis comprises the following steps: first, mixing arbuscular mycorrhizal fungi composed of Funneliformis mosseae and Glomus intraradices at a volume ratio of 1:1 with a commercial seedling medium at a ratio of 1:10 (w / w), and growing pepper seedlings; transplanting the pepper seedlings when they have 4 to 6 true leaves; and 7 days after transplanting the peppers, inoculating the peppers with an effective viable count of 2×10 8 cfu / mL suspension of Priesteria gigantea, 2 ml per plant; in the early stage of pepper fruit setting, spray the leaves with foliar fertilizer containing the Priesteria gigantea suspension, diluted 200 times, once every 7 days, for a total of 2 sprays, each application rate of 40 L / mu; finally, 10 days before pepper picking, spray the leaves with a concentration of 15 mg / L abscisic acid once, with an application rate of 40 L / mu;

[0024] The preparation steps of the Priesteria gigantea suspension are as follows: under sterile conditions, the Priesteria gigantea strain is inoculated onto LB solid medium and cultured at a constant temperature of 28°C; then a single colony is picked and inoculated into LB liquid medium, and cultured at 28°C and 180 rpm for at least 24 hours, and examined under a microscope until the effective viable count reaches 2×10 8 cfu / mL, and the bacterial suspension was obtained;

[0025] The steps for preparing foliar fertilizer containing Priesteria gigantea suspension are as follows: according to the steps for preparing Priesteria gigantea suspension, the effective viable bacteria count is 2×10 8 cfu / mL of bacterial suspension, inoculate a single colony of the bacterial suspension into LB liquid medium, shake culture for 6 hours, add 4g sodium phosphate and 20g salicylic acid per liter of bacterial suspension, and then continue shaking culture for 20 hours. Microscopic examination was performed until the effective viable count reached 6×10 8cfu / mL, and finally 0.05% sodium benzoate was added to obtain the foliar fertilizer of Priesteria gigantea suspension.

[0026] Example 2

[0027] A cultivation method for promoting pepper growth and capsaicin synthesis comprises the following steps: first, mixing arbuscular mycorrhizal fungi composed of Funneliformis mosseae and Glomus etunicatum at a volume ratio of 1:1 with a commercial seedling medium at a ratio of 1:15 (w / w) to grow pepper seedlings; transplanting the pepper seedlings when they have 4 to 6 true leaves; and 8 days after transplanting, inoculating the peppers with an effective viable count of 3×10 8 cfu / mL suspension of Priesteria gigantea, 2 ml per plant; in the early stage of pepper fruit setting, spray the leaves with foliar fertilizer containing the Priesteria gigantea suspension, the foliar fertilizer is diluted 250 times when spraying, spray once every 8 days, a total of 3 sprays, each application amount is 30L / mu; finally, 12 days before pepper picking, spray the leaves with a concentration of 20mg / L abscisic acid once, the application amount is 30L / mu;

[0028] The preparation steps of the Priesteria gigantea suspension are as follows: under sterile conditions, the Priesteria gigantea strain is inoculated onto LB solid medium and cultured at a constant temperature of 29°C; then a single colony is picked and inoculated into LB liquid medium, and cultured at 29°C and 190 rpm for at least 24 hours, and examined under a microscope until the effective viable count reaches 3×10 8 cfu / mL, and the bacterial suspension was obtained.

[0029] The steps for preparing foliar fertilizer containing Priesteria gigantea suspension are as follows: according to the steps for preparing Priesteria gigantea suspension, the effective viable bacteria count is 3×10 8 cfu / mL of bacterial suspension, a single colony of the bacterial suspension was inoculated into LB liquid medium, and after shaking culture for 7 hours, 6g of sodium phosphate and 25g of salicylic acid were added to each liter of bacterial suspension, and then shaking culture was continued for 20 hours. The culture was examined under a microscope until the effective viable count reached 8×10 8 cfu / mL, and finally 0.75% sodium benzoate was added to obtain the foliar fertilizer of Priesteria gigantea suspension.

[0030] Example 3

[0031] A cultivation method for promoting pepper growth and capsaicin synthesis comprises the following steps: first, mixing arbuscular mycorrhizal fungi composed of Glomus intraradices and Glomus etunicatum at a volume ratio of 1:1 with a commercial seedling medium at a ratio of 1:20 (w / w), and growing pepper seedlings; transplanting the pepper seedlings when they have 4-6 true leaves; and 10 days after transplanting the peppers, inoculating the peppers with an effective viable count of 4×10 8 cfu / mL suspension of Priesteria gigantea, 2 ml per plant; at the early stage of pepper fruit setting, spray the leaves with foliar fertilizer containing the Priesteria gigantea suspension, the foliar fertilizer is diluted 300 times when sprayed, spray once every 10 days, for a total of 3 sprays, each application amount is 50 L / mu; finally, 15 days before pepper picking, spray the leaves with a concentration of 25 mg / L abscisic acid once, the application amount is 50 L / mu;

[0032] The preparation steps of the Priesteria gigantea suspension are as follows: under sterile conditions, the Priesteria gigantea strain is inoculated onto LB solid medium and cultured at a constant temperature of 30°C; then a single colony is picked and inoculated into LB liquid medium, and cultured at 30°C and 200 rpm for at least 24 hours, and examined under a microscope until the effective viable count reaches 4×10 8 cfu / mL, and the bacterial suspension was obtained;

[0033] The steps for preparing foliar fertilizer containing Priesteria gigantea suspension are as follows: according to the steps for preparing Priesteria gigantea suspension, the effective viable bacteria count is 4×10 8 cfu / mL of bacterial suspension, a single colony of the bacterial suspension was inoculated into LB liquid medium, and after shaking culture for 8 h, 8 g of sodium phosphate and 30 g of salicylic acid were added to each liter of bacterial suspension, and then shaking culture was continued for 20 h. The culture was examined under a microscope until the effective viable count reached 10 × 10 8 cfu / mL, and finally 0.1% sodium benzoate was added to obtain the foliar fertilizer of Priesteria gigantea suspension.

[0034] Example 4

[0035] This embodiment differs from embodiment 2 in that the arbuscular mycorrhizal fungi only include Pseudomonas mosseae, and the rest are the same.

[0036] Example 5

[0037] This embodiment differs from embodiment 2 in that the arbuscular mycorrhizal fungi only include Glomus intraradicus, and the rest are the same.

[0038] Example 6

[0039] This embodiment differs from embodiment 2 in that the arbuscular mycorrhizal fungi only include Glomus juvenileus, and the rest are the same.

[0040] Test example:

[0041] In order to further illustrate the promotion and application value of the present invention, the effects of promoting growth and increasing the capsaicin content in the examples were verified.

[0042] (1) Verification of the growth-promoting effect of pepper seedlings

[0043] Experimental design: The experimental design includes 21 treatments, with 3 replicates for each treatment. The specific experimental design is as follows:

[0044] Treatment 1: Planting was carried out according to the cultivation method of Example 1.

[0045] Treatment 2: Planting was carried out according to the cultivation method of Example 2.

[0046] Treatment 3: Planting was carried out according to the cultivation method of Example 3.

[0047] CK1: Planting was carried out according to the cultivation method of Example 1, but the arbuscular mycorrhizal fungi were sterilized.

[0048] CK2: Planting was carried out according to the cultivation method of Example 2, but the arbuscular mycorrhizal fungi were sterilized.

[0049] CK3: Planting was carried out according to the cultivation method of Example 3, but the arbuscular mycorrhizal fungi were sterilized.

[0050] CK1-1: The cultivation method was followed in Example 1, except that Priesteria gigantea was sterilized.

[0051] CK1-2: Planting was carried out according to the cultivation method of Example 2, but Priesteria gigantea was sterilized.

[0052] CK1-3: Planting was carried out according to the cultivation method of Example 3, but Priesteria gigantea was sterilized.

[0053] CK2-1: Planting was carried out according to the cultivation method of Example 1, but the arbuscular mycorrhizal fungi and Priesteria gigantea were sterilized.

[0054] CK2-2: Planted according to the cultivation method of Example 2, but the arbuscular mycorrhizal fungi and Priesteria gigantea were sterilized.

[0055] CK2-3: Planting was carried out according to the cultivation method of Example 3, but the arbuscular mycorrhizal fungi and Priesteria gigantea were sterilized.

[0056] CK3-1: Planted according to the cultivation method of Example 1, but not inoculated with arbuscular mycorrhizal fungi.

[0057] CK3-2: Planted according to the cultivation method of Example 2, but not inoculated with arbuscular mycorrhizal fungi.

[0058] CK3-3: Planted according to the cultivation method of Example 3, but not inoculated with arbuscular mycorrhizal fungi.

[0059] CK4-1: Planted according to the cultivation method of Example 1, but without inoculating Priesteria gigantea.

[0060] CK4-2: Planted according to the cultivation method of Example 2, but without inoculating Priesteria gigantea.

[0061] CK4-3: Planted according to the cultivation method of Example 3, but without inoculating Priesteria gigantea.

[0062] CK5-1: Planted according to the cultivation method of Example 1, but not inoculated with arbuscular mycorrhizal fungi and Priesteria gigantea.

[0063] CK5-2: Planted according to the cultivation method of Example 2, but not inoculated with arbuscular mycorrhizal fungi and Priesteria gigantea.

[0064] CK5-3: Planted according to the cultivation method of Example 3, but not inoculated with arbuscular mycorrhizal fungi and Priesteria gigantea.

[0065] A pot experiment was conducted to raise and transplant pepper seedlings according to the above experimental design. 20 days after the pepper seedlings were transplanted, the plant height, stem diameter and chlorophyll spad value of the peppers were measured. SPSS software was used for statistical analysis. The results are shown in Table 1.

[0066] Table 1 Effects of arbuscular mycorrhizal fungi and Priesteria gigantea on growth indices of pepper seedlings

[0067]

[0068]

[0069] Note: Different lowercase letters abcde indicate significant differences at the 0.05 level under different planting methods. Different letters mark the values, indicating that there is a significant difference between the two. The same letters in the marking indicate no significant difference (P<0.05). The specific marking rules are as follows: first arrange the average values ​​of each treatment from large to small from top to bottom, then mark the letter a after the largest average value, and compare the average value with the following average values ​​in turn. Any insignificant difference is marked with the same letter a until a mean value with a significant difference is marked with the letter b; then use the mean value marked with the letter b as the standard and compare it with the average values ​​above it that are larger than it. Any insignificant difference is marked with b again until it is significant; and so on, marking continues until the smallest average value is marked and the comparison is completed. In this way, any average value with one identical letter means that the difference is not significant, and any average value without the same letter means that the difference is significant. The same below.

[0070] As shown in Table 1, after inoculation with arbuscular mycorrhizal fungi and Priesteria gigantea (treatments 1 to 3) according to the cultivation method of the present invention, the plant height, stem diameter and chlorophyll spad value of pepper seedlings were significantly higher than those of the treatments without inoculation with arbuscular mycorrhizal fungi (CK3-1 to 3-3), the treatment without inoculation with Priesteria gigantea (CK4-1 to 4-3), the treatment without inoculation with either (CK5-1 to 5-3), the treatment with only inoculation with arbuscular mycorrhizal fungi and sterilization (CK1 to 3), the treatment with Priesteria gigantea and sterilization (CK1-1 to 1-3) and the treatment with both sterilization (CK2-1 to 2-3); in addition, the treatments without inoculation with arbuscular mycorrhizal fungi and Priesteria gigantea were significantly higher than those of the treatments without inoculation with arbuscular mycorrhizal fungi and Priesteria gigantea (CK4-1 to 4-3), the treatment without inoculation with either (CK5-1 to 5-3), the treatment with only inoculation with arbuscular mycorrhizal fungi and sterilization (CK1 to 3), the treatment with Priesteria gigantea and sterilization (CK1-1 to 1-3) and the treatment with both sterilization (CK2-1 to 2-3). The plant height, stem diameter, and chlorophyll spad values ​​of pepper seedlings treated with Priesteria gigantea (CK5-1 to 5-3) were significantly lower than those in the treatments without single bacterial inoculation (CK3-1 to 3-3, CK4-1 to 4-3). Furthermore, the plant height, stem diameter, and chlorophyll spad values ​​of pepper seedlings inoculated with only arbuscular mycorrhizal fungi and sterilized with Priesteria gigantea (CK2-1 to 2-3) were also significantly lower than those inoculated with only single bacterial sterilization (CK1 to 3, CK1-1 to 1-3). Therefore, this suggests that arbuscular mycorrhizal fungi and Priesteria gigantea interact to a certain extent, significantly promoting the growth of pepper seedlings. Furthermore, Examples 4 to 6 of the present invention also significantly promoted the growth of pepper seedlings, though the effect was slightly less pronounced than that of Example 2.

[0071] (2) Verification of the effect of increasing capsaicin content

[0072] Test materials: Hangzhou pepper (Hangjiao No. 1), string pepper (Meila 3+1), screw pepper (Hanyu 1677)

[0073] Experimental design: The experimental design includes 15 treatments, with 3 replicates for each treatment. The specific experimental design is as follows:

[0074] Treatment 1: Planting was carried out according to the cultivation method of Example 1.

[0075] Treatment 2: Planting was carried out according to the cultivation method of Example 2.

[0076] Treatment 3: Planting was carried out according to the cultivation method of Example 3.

[0077] CK1: Planting was carried out according to the cultivation method of Example 1, but no foliar fertilizer was sprayed.

[0078] CK2: Planting was carried out according to the cultivation method of Example 2, but no foliar fertilizer was sprayed.

[0079] CK3: Planting was carried out according to the cultivation method of Example 3, but no foliar fertilizer was sprayed.

[0080] CK1-1: Plant according to the cultivation method of Example 1 and spray with sterilized foliar fertilizer.

[0081] CK1-2: Plant according to the cultivation method of Example 2 and spray with sterilized foliar fertilizer.

[0082] CK1-3: Plant according to the cultivation method of Example 3 and spray sterilized foliar fertilizer.

[0083] CK2-1: Planting was carried out according to the cultivation method of Example 1, but salicylic acid was used instead of foliar fertilizer.

[0084] CK2-2: Planting was carried out according to the cultivation method of Example 2, but salicylic acid was used instead of foliar fertilizer.

[0085] CK2-3: Planting was carried out according to the cultivation method of Example 3, but salicylic acid was used instead of foliar fertilizer.

[0086] CK3-1: Planting was carried out according to the cultivation method of Example 1, and only the suspension of Priesteria gigantea in the foliar fertilizer was sprayed.

[0087] CK3-2: Planting was carried out according to the cultivation method of Example 2, and only the suspension of Priesteria gigantea in the foliar fertilizer was sprayed.

[0088] CK3-3: Planting was carried out according to the cultivation method of Example 3, and only the suspension of Priesteria gigantea in the foliar fertilizer was sprayed.

[0089] Arbuscular mycorrhizal fungi and commercial seedling medium were evenly mixed according to the experimental design to raise pepper seedlings. When the pepper seedlings grew to 4-6 true leaves, seedlings with consistent growth were selected and transplanted into pots. Each pot was filled with 12 kg of medium. To ensure normal growth and development of the peppers, each pot was fertilized with 6.4 g of nitrogen, 2.13 g of phosphorus oxide, and 3.1 g of potassium oxide as base fertilizer. Cultivation and management were then carried out according to the experimental design. At the time of harvest, the capsaicin content of the pepper fruits was determined and statistically analyzed using SPSS software. The results are shown in Table 2.

[0090] Table 2 Effect of foliar fertilizer on capsaicin content in different pepper varieties

[0091]

[0092] Note: Different lowercase letters abcd indicate significant differences at the 0.05 level among different planting methods.

[0093] As shown in Table 2, the capsaicin content of peppers grown according to the cultivation method of the present invention (Treatments 1 to 3) was significantly higher than that of the treatments without foliar fertilizer (CK1 to 3), the treatments with sterilized foliar fertilizer (CK1-1 to 1-3), the treatments with salicylic acid instead of foliar fertilizer (CK2-1 to 2-3), and the treatments with only a suspension of Priesteria gigantea in the foliar fertilizer (CK3-1 to 3-3). Therefore, it can be shown that, to a certain extent, the synergistic effect of arbuscular mycorrhizal fungi, Priesteria gigantea, and foliar fertilizers containing Priesteria gigantea and salicylic acid can significantly increase the capsaicin content of pepper fruits when grown according to the cultivation method of the present invention. In addition, Examples 4 to 6 of the present invention also have a significant promoting effect on increasing capsaicin content, but the effect is slightly worse than that of Example 2.

[0094] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A cultivation method for promoting pepper growth and capsaicin synthesis, characterized in that: The following steps are involved: Arbuscular mycorrhizal fungi are inoculated when pepper seedlings are being grown, and a suspension of Priesteria gigantea is inoculated 7-10 days after the pepper seedlings are transplanted. Foliar fertilizer containing the suspension of Priesteria gigantea is sprayed on the leaves at the early stage of fruit setting, and finally abscisic acid is sprayed on the leaves 10-15 days before the peppers are picked. The arbuscular mycorrhizal fungi is Pseudomonas mosseae ( Funneliformis mosseae ), Glomus intraradices ( Glomus intraradices ) and Glomus juveniles ( Glomus etunicatum ) one or two of the above; the inoculation amount of the arbuscular mycorrhizal fungi is 10%-20% of the mass of the seedling medium when raising pepper seedlings; the inoculation amount of the Priesteria gigantea suspension is 1-3 ml / plant, and the effective viable bacteria count is 2-4×10 8 cfu / mL; the foliar fertilizer is sprayed once every 7-10 days, the dilution ratio is 200-300 times, sprayed 2-3 times, and the application amount is 30-50L / mu; the abscisic acid spraying concentration is 15-25mg / L, and the application amount is 30-50L / mu.

2. The method for promoting pepper growth and capsaicin synthesis according to claim 1, wherein: Pepper seedlings are transplanted when they have 4-6 true leaves.

3. The cultivation method for promoting pepper growth and capsaicin synthesis according to claim 1, wherein: The preparation process of the Priesteria gigantea suspension is as follows: under sterile conditions, the Priesteria gigantea strain is inoculated onto a solid culture medium, cultured at a constant temperature of 28-30°C, then a single colony is picked and inoculated into a liquid culture medium, cultured at 28-30°C and 180-200 rpm for at least 24 hours, and examined under a microscope until the effective viable count reaches 2-4×10 8 cfu / mL, and the suspension of Priesteria gigantea was obtained.

4. The cultivation method for promoting pepper growth and capsaicin synthesis according to claim 1, wherein: The foliar fertilizer preparation process containing the suspension of Priesteria gigantea is as follows: a single colony of the suspension of Priesteria gigantea is picked and inoculated into a liquid culture medium, and after shaking culture for 6-8 hours, 4-8 g of sodium phosphate and 20-30 g of salicylic acid are added to each liter of the suspension in sequence, and then the shaking culture is continued for 20-24 hours, and the suspension is examined under a microscope until the effective viable count reaches 6-10×10 8 cfu / mL, and finally 0.05-0.1% of the total mass of sodium benzoate was added to obtain a foliar fertilizer containing Priesteria gigantea suspension.

5. The cultivation method for promoting pepper growth and capsaicin synthesis according to claim 4, characterized in that: The liquid culture medium is LB liquid culture medium.

6. The cultivation method for promoting pepper growth and capsaicin synthesis according to claim 3, characterized in that: The solid culture medium is LB solid culture medium; the liquid culture medium is LB liquid culture medium.

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