10 results about "Serodiagnoses" patented technology

The present application relates to a kind of monoclonal antibody DIVA55 for swine fever virus in the field of medical preparation and its preparation method and application.The monoclonal antibody DIVA55 or its antigen-binding fragment of the present application includes heavy chain variable region and light chain variable region;The amino acid sequences of LCDR1, LCDR2 and LCDR3 in the light chain variable region of the antibody are as shown in the 24th to 34th, 50th to 56th, 89th to 97th of SEQ ID No:4;The amino acid sequences of HCDR1, HCDR2 and HCDR3 in the heavy chain variable region of the antibody are as shown in the 31st to 35th, 50th to 65th, 95th to 102nd of SEQ ID No:5.The monoclonal antibody of the present application can be used in the field such as swine fever E2 protein marker subunit vaccine immunization and wild strain serological diagnosis, swine fever vaccine immunization effect evaluation and related experiments, provides antibody resource for CSF prevention and control and purification.

The invention discloses a novel anti-echinococcus monoclonal antibody, a hybridoma cell strain and application of the novel anti-echinococcus monoclonal antibody, and belongs to the technical field of biomedicine and immunology. The preservation number of the hybridoma cell strain Anti-Em-11 disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 46598. According to the present invention, the specific monoclonal antibody is successfully prepared and identified, and Western blot and indirect immunofluorescence experiments prove that the obtained monoclonal antibody has high specificity on the recombinant protein. The invention lays a solid foundation for serological diagnosis of the echinococcosis and remarkably enriches the existing serological detection technical system.

The invention relates to the field of veterinary protozoology and methods for producing antigens, particularly for the diagnosis of Surra Su-Auru in animals. The objective of the invention is to develop a method for producing a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals. The problem is solved by the fact that the method for obtaining trypanosome antigen for serological diagnostics of Surra (Su-Auru) in animals involves infecting rabbits intratesticularly with live trypanosomes of the T species. evansi, removal of the spleen 2-3 days after infection, total bleeding 8-10 days after splenectomy (parasitemia -80-90%), blood is collected in a wide vessel with 2% sodium citrate in physiological solution with constant stirring with a glass rod, at the rate of 1 part blood to 2 parts solution, settling of citrated blood in the refrigerator for 18-24 hours, centrifugation and separation of the middle layer from the sediment from the supernatant, 2-3 times washing of trypanosomes in physiological solution, at 3000-5000 rpm, for 10 minutes each, disintegration of the centrifugate by ultrasound at a frequency of 22 kHz, power of 60 W / cm2 for 8-12 minutes, followed by centrifugation of the disintegrate at 8000-10000 rpm for 20-30 minutes and collection of sediment (endoantigen), treatment of the supernatant with TCA, collection of the precipitated sediment (exoantigen), batches of sediment are mixed 10:1. The technical result provided by the utility model is expressed in the development of a method for obtaining a highly sensitive trypanosomal antigen for the serological diagnosis of Surra (Su-Auru) in animals.

The application discloses a monoclonal antibody of a porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody can specifically recognize a linear B cell epitope on the VP6 protein, and a core sequence of the epitope is TTRLSFQLVR. The antibody has high affinity and high specificity, and can cross-react with porcine rotaviruses of various genotypes such as G5, G9 and G11. The application further includes an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in detection of porcine rotaviruses, development of vaccines and research on functions of viral proteins. The application provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of porcine rotaviruses.

This invention belongs to the field of reagent detection technology and discloses a diagnostic kit for P200 type pemphigus. The kit comprises human dermal extract protein strips, LM521 / LM421 / LM411 / LM111 recombinant protein strips, a positive control, a negative control, enzyme-labeled IgG antibody, enzyme-labeled IgA antibody, reaction buffer, washing buffer, and enzyme matrix solution. This invention simplifies the complex immunoblotting technique, using human dermal extract and various LMγ1 recombinant protein mixtures as antigens, and simultaneously analyzing IgG and IgA autoantibodies. It comprehensively solves the serological diagnostic problem of P200 type pemphigus in one step.

The invention provides a mycoplasma bovis specific antigen epitope peptide and application thereof in antibody detection, and the amino acid sequence of the antigen epitope peptide is SEQ ID NO: 1. Bioinformatics prediction and serological verification are carried out on mycoplasma bovis immunogenic protein, the antigen epitope peptide highly associated with antibody reaction specificity is obtained through screening, a high-specificity mycoplasma bovis antibody detection method can be established by using the epitope peptide, the detection specificity is 100%, the sensitivity is 98.8%, and the detection time is short. And an effective tool is provided for serological diagnosis of mycoplasma bovis infection.

The invention relates to the technical field of antibodies, and particularly provides an antibody specifically bound with African swine fever virus p62 protein and application. The antibody or the antigen binding fragment thereof comprises a heavy chain variable region with an amino acid sequence as shown in SEQ ID NO.1 and a light chain variable region with an amino acid sequence as shown in SEQ ID NO.3, p62 protein can be recognized with high specificity, and the blocking rate of the antibody reaches 90% or above. The antibody can exist in various forms such as a monoclonal antibody and a single-chain antibody, and biological materials such as coding nucleic acid, expression vectors and recombinant cells of the antibody can be produced on a large scale. Based on the antibody or the antigen binding fragment thereof, the antibody or the antigen binding fragment thereof can be used for preparing an African swine fever virus detection product, is especially suitable for constructing a blocking ELISA antibody detection kit with high sensitivity and strong specificity, and provides an effective tool for serological diagnosis and monitoring of African swine fever.

The invention belongs to the technical field of biological detection, and particularly relates to a monoclonal antibody 5H4 for detecting a classical swine fever virus antibody and application of the monoclonal antibody 5H4. A heavy chain variable region of the monoclonal antibody 5H4 comprises three complementarity determining regions, and amino acid sequences of the complementarity determining regions are respectively shown as SEQ ID NO.1 to SEQ ID NO.3; the light chain variable region comprises three complementarity determining regions, and the amino acid sequences of the three complementarity determining regions are respectively shown as SEQ ID NO.4 to SEQ ID NO.6. The antibody can recognize a key antigen epitope of the classical swine fever virus E2 protein, and can be competitively blocked by a specific antibody in animal serum. A blocking ELISA detection method established based on the antibody has a detection result consistent with that of an international mainstream commercial kit, and has excellent specificity, sensitivity and repeatability. The invention provides an efficient and reliable tool for serological diagnosis of classical swine fever virus infection and vaccine immune effect evaluation.

The application discloses a novel coronavirus S protein specific B cell antigen epitope polypeptide and application thereof, and belongs to the technical field of molecular immunology. In order to provide a novel coronavirus protein polypeptide antigen with immunoprotective function or serodiagnosis value. The amino acid sequence of the antigen epitope polypeptide disclosed by the application is shown as SEQ ID NO:1 or SEQ ID NO:2. The SARS-CoV-2 S protein specific B cell antigen epitope synthetic polypeptide, the synthetic epitope polypeptide coupling antigen and the antigen epitope fusion expression protein disclosed by the application can be used as antigens to specifically detect the anti-SARS-CoV-2 S protein antibodies generated after the body is immunized or infected, can be used for SARS-CoV-2 infection diagnosis, vaccine immunization effect evaluation and as a vaccine component to induce specific antibodies.

The invention relates to the technical field of biology, in particular to an antibody specifically bound with African swine fever virus E165R protein or an antigen binding fragment thereof and application of the antibody or the antigen binding fragment. The antibody or the antigen binding fragment thereof comprises a heavy chain variable region as shown in SEQ ID NO: 1 and a light chain variable region as shown in SEQ ID NO: 3. High blocking activity and excellent specificity are realized. The invention also provides nucleic acid molecules encoding the antibody or the antigen binding fragment thereof, expression cassettes, recombinant vectors and host cells. A blocking ELISA detection kit established on the basis of the antibody or the antigen binding fragment thereof can realize high-sensitivity detection of the E165R antibody in porcine serum through a competitive binding principle of coated E165R protein and an enzyme-labeled antibody. The invention provides a key tool for structural analysis, serological diagnosis and genetic engineering antibody development of the African swine fever virus E165R protein.