44 results about "Linear epitope" patented technology

The invention discloses a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof, and relates to the field of genetic engineering, in particular to a hybridoma cell strain, a monoclonal antibody, a linear epitope antigen and application thereof. The preservation number of the hybridoma cell strain P27-A1 is CGMCC (China General Microbiological Culture Collection Center) No. 46352. The hybridoma cell strain P27-A1 is prepared from feline leukemia virus p27, and a monoclonal antibody secreted by the hybridoma cell strain P27-A1 recognizes a linear epitope antigen of feline leukemia virus p27 protein; the kit for the feline leukemia virus p27 comprises the monoclonal antibody A1 secreted by the hybridoma cell strain P27-A1. The monoclonal antibody secreted by the hybridoma cell strain can greatly improve the sensitivity of the p27 protein for detecting feline leukemia virus. The invention provides the monoclonal antibody secreted by the hybridoma cell strain for identification, and the monoclonal antibody can be used for preparing a diagnostic kit such as a colloidal gold test strip.

The invention discloses a type 2 GAstV capsid protein monoclonal antibody and an antigen epitope peptide of the type 2 GAstV capsid protein monoclonal antibody. The monoclonal antibody is prepared by immunizing a mouse with capsid protein GAstV Cap of the type 2 goose astrovirus as an antigen, and is named as 1F1; the monoclonal antibody 1F1 comprises a heavy chain variable region and a light chain variable region, and the amino acid sequences of the heavy chain variable region and the light chain variable region are sequentially shown as SEQ ID No.3 and SEQ ID No.5. In order to determine the B cell antigen epitope of the screened monoclonal antibody, the invention also constructs a GAstV Cap truncated fusion protein, and the antigen recognition epitope is determined through indirect ELISA and Western blotting tests, so that screening is carried out step by step until the shortest linear epitope recognized by the monoclonal antibody is determined, and the amino acid sequence of the shortest linear epitope is shown as SEQ ID No.10. Therefore, the monoclonal antibody and the antigen epitope of the monoclonal antibody can be applied to research on structures and functions of GAstV capsid proteins and preparation of serological diagnostic tools and epitope vaccines.

The present application relates to the field of biotechnology and in vitro diagnosis, and relates to a monoclonal antibody of eosinophil peroxidase and application thereof. The antibody of the present application is mainly used for detecting the content of eosinophil peroxidase by western blot. The monoclonal antibody of eosinophil peroxidase of the present application is prepared by immunizing Balb / c mice with recombinant eosinophil peroxidase as an immunogen, then fusing the spleen with myeloma cells NS1, and screening and purifying. The prepared monoclonal antibody of eosinophil peroxidase specifically recognizes the linear epitope of eosinophil peroxidase.

The invention belongs to the technical field of fish immunodetection, and particularly relates to antigenic epitope polypeptide of anti-channa argus IgM, a polyclonal antibody as well as a preparation method and application of the polyclonal antibody. Specifically, the invention successfully constructs a preparation technology system based on the snakehead IgM specific antigen epitope polypeptide and the corresponding polyclonal antibody. Meanwhile, through combination of bioinformatics epitope prediction and immunogenicity optimization, the linear epitope polypeptide with high reactogenicity is successfully screened and obtained. And finally constructing to obtain the anti-channa argus IgM polyclonal antibody, and the verification shows that the polyclonal antibody has high specific recognition capability on channa argus mucus IgM protein. The successful preparation of the antibody fills the blank of a reagent for detecting the mucosal immune globulin of the freshwater economic fish, and provides a core reagent basis for establishing standardized detection platforms such as fish vaccine titer evaluation and immune response dynamic monitoring, so that the antibody has good practical application value.

The present invention relates to the technical field of immunopharmaceutical R & D, and discloses a BCE prediction method based on a hybrid deep learning strategy, which includes the following steps: Step 1, construct a BCE prediction model; Step 2, input a protein sequence into the BCE prediction model; Step 3, use the prediction model to output the result of whether the protein sequence is a BCE. The BCE prediction model in Step 1 includes a database, a feature extraction module, a feature processing module, and a forward neural network module; wherein: the database is used to obtain a training data set; the feature extraction module is used to extract features from the data and obtain four groups of features; the feature processing module is used to process the extracted features. The deep learning prediction method proposed by the present invention represents a protein with sequence features and structural features, and uses a four-channel deep neural network to extract sequence information and structural information respectively, and the same model can be used to predict linear epitopes.

A tumor vaccine for treating esophageal squamous cell carcinoma and other cancers, and a composition thereof. Specifically, provided is a polypeptide, which contains one or more antigen fragments selected from: ACTL8, BRDT, FOXI3, GNGT1, SMC1B, PLAC1, MAGEA1, MAGEA3, MAGEA4, MAGEA6, MAGEA10 and MAGEA11, wherein the one or more antigen fragments are linked via a linker peptide, and the antigen fragments contain one or more antigenic epitope peptides. Furthermore, provided are a linear epitope peptide, a nucleic acid encoding the polypeptide and the linear epitope peptide, and the use of the polypeptide or the linear epitope peptide in the preparation of a drug for preventing or treating cancers such as esophageal squamous cell carcinoma.

This invention discloses monoclonal antibodies that recognize linear B-cell epitopes in the conserved extracellular domain of pseudorabies virus (PRV) gE protein and their applications. Using recombinant PRV gE protein extracellular domain protein as an immunogen, hybridoma cell lines were prepared. Combining dot blot and peptide scanning techniques, two monoclonal antibodies, 4E5D7 and 8F6B2, were screened to recognize the 69-93aa region of the conserved extracellular domain of the PRV gE protein. Both antibodies achieved a titer of 1:2048000 and specifically recognized the native PRV wild-type gE protein, showing no cross-reactivity with gE gene-deleted vaccines. The monoclonal antibodies of this invention fill the gap in detection reagents targeting the precise epitopes of the 69-81aa and 79-93aa extracellular domains of the PRV gE protein, thus improving the linear B-cell epitope map of the PRV gE protein. These monoclonal antibodies can be used for research on the pathogenesis of PRV and the development of specific detection reagents for PRV wild-type strains, possessing significant application value and translational prospects in basic scientific research, veterinary clinical testing, and animal disease prevention and control.

The invention belongs to the technical field of immunology and in vitro diagnosis, and relates to a monoclonal antibody for specifically recognizing bluetongue virus VP5 protein. According to the invention, BTV-1 recombinant VP5 protein with deletion of 1-79 amino acids at the N terminal is obtained through expression; immunizing a mouse with the protein, screening out a monoclonal antibody 5H11 aiming at the BTV-1VP5 protein, and obtaining heavy chain and light chain variable region sequences for coding the antibody; the monoclonal antibody 5H11 can be subjected to a specific reaction with BTV-1 VP5 protein, and can be used for specifically recognizing natural VP5 protein in BTV-1 infected cells; by carrying out truncation expression on the BTV-1VP5 protein twice, the B cell linear epitope identified by 5H11 on the VP5 protein is identified, and a foundation is laid for deeply researching the structural function of the BTV VP5 protein and researching and developing a novel vaccine.

The invention provides a monoclonal antibody of a canine distemper virus and application thereof, and belongs to the technical field of animal medicine detection. The antibody provided by the invention has ultrahigh binding affinity to the CDV H protein of the canine distemper virus, and the linear epitope is the 373-381 site of the CDV H protein. According to the kit disclosed by the invention, the nitrocellulose membrane is adopted to coat the canine virus specific antibody and the fluorescent marker, so that the virus in a canine sample can be rapidly detected. The adopted antibody is prepared through a genetic engineering means, and the detection sensitivity of the antibody is obviously superior to that of a conventional colloidal gold test strip and an ELISA detection method. The antibody disclosed by the invention keeps excellent stability in a high-temperature environment, and shows an extremely low cross reaction characteristic to pathogens such as CAV. By matching with a special fluorescence detection device, the technology can complete efficient detection of the canine distemper virus in a short time, and is suitable for clinical diagnosis and treatment and on-site rapid screening scenes.

The application provides preparation and application of a diagnostic monoclonal antibody for a new epitope of human neutrophil lipocalin (HNL). Two new HNL protein conformation epitopes and one new HNL linear epitope are designed and provided, and an anti-HNL antibody with higher binding affinity and better binding specificity is screened to combine the new epitopes. The anti-HNL antibody of the application can be used for preparation of an HNL detection reagent and kit, and has higher sensitivity and specificity when used for detecting HNL protein in a sample, and can be applied to distinguishing bacterial infection and viral infection.

The invention discloses an N protein recombinant antigen for detecting a new bunyavirus antibody and application of the N protein recombinant antigen. The N protein recombinant antigen comprises an N protein and a peptide fragment containing a linear epitope in the N protein. The N protein recombinant antigen completely covers SFTSV antibody recognition sites and has high activity, and the prepared protein is high in purity, stable in structure and good in batch-to-batch stability.

The present invention relates to a method for predicting immunogenic epitopes and an apparatus using the same. The method for predicting immunogenic epitopes according to the present invention includes the steps of: calculating the degree of influence of factors involved in a biological process of a tumor cell for an epitope and characteristics of the epitope on the immunogenicity of the epitope; performing at least one of standardization and normalization on the calculated value; and inputting the value after the standardization and normalization into a pre-trained artificial intelligence model to predict the immunogenicity of the epitope. The biological process may include an antigen processing stage, an antigen presentation stage, an immune stage, and a tumor microenvironment, and the factors involved in the tumor microenvironment may be at least one of an inflammatory response, a B-cell linear epitope, and a B-cell conformational epitope.

The invention relates to the field of biotechnology and in vitro diagnosis, in particular to a monoclonal antibody of eosinophilic granulocyte peroxidase and application of the monoclonal antibody. The antibody disclosed by the invention is mainly used for detecting the content of peroxidase in eosinophilic granulocytes by using western blot. The monoclonal antibody for resisting eosinophilic granulocyte peroxidase is prepared by the following steps: taking recombinant eosinophilic granulocyte peroxidase as an immunogen to immunize a Balb / c mouse, then fusing the spleen with a myeloma cell NS1, and screening and purifying to obtain the monoclonal antibody for resisting eosinophilic granulocyte peroxidase. The prepared monoclonal antibody for resisting the eosinophilic granulocyte peroxidase specifically recognizes the linear epitope of the eosinophilic granulocyte peroxidase.

The present invention discloses an oral cancer marker linear epitope fusion peptide and an application thereof. The oral cancer marker linear epitope fusion peptide comprises a fusion peptide 1 and a fusion peptide 2; the nucleotide sequence of the fusion peptide 1 is: SKDQIKKLTSLKNKLERRQNRSQNPVQPIGPQTPKACDSK; the nucleotide sequence of the fusion peptide 2 is: SKDQIKKLTSLKNKLERRQNEDERWTNNFREYNL; the oral cancer marker linear epitope fusion peptide can be fused with a T cell epitope to promote the occurrence of an immune response. The generated antiserum has high affinity and specificity, laying a good foundation for the further preparation of high-affinity monoclonal antibodies and the development of detection kits. The fusion peptide 1 and the fusion peptide 2 can replace the full-length MMP-1 as a calibrator of the detection kit through chemical coupling treatment.

The invention discloses a B cell linear epitope prediction method and device and a storage medium, and belongs to the technical field of immunity and drug development. The method for predicting the linear epitope of the B cell comprises the following steps: respectively preprocessing sequences to be detected to obtain target input sequences; performing feature coding on each amino acid residue in the obtained target input sequence, and outputting a coding matrix of each sequence; inputting the obtained local feature matrix into a two-layer or five-layer BiGRU module in a trained B cell linear epitope prediction model, and outputting a global feature matrix of each sequence; and inputting the obtained global feature matrix of each sequence into three full connection layers in the B cell linear epitope prediction model, and outputting the probability that the to-be-detected sequence is the B cell epitope. In addition, the invention also provides a B cell linear epitope prediction device and a storage medium. According to the prediction method provided by the invention, the prediction accuracy of the linear epitope of the B cell is remarkably improved.

An antibody that can specifically bind to semenogelin-1 (SEMG1) and semenogelin-2 (SEMG2) and regulate the binding of said proteins with a receptor (LILRB2, LILRB4, CD27, or TIGIT) thereof. It is proven that SEMG1 and SEMG2 have a regulating effect on immune cells, and it is proven that the antibody has a neutralizing effect. A specific linear epitope located on SEMG1 and SEMG2 proteins is provided, and it is proven that an antibody recognizing said epitope has a stronger neutralizing function, can more effectively remove an immunosuppressive effect mediated by SEMG1 and SEMG2, and promotes immune cells to inhibit and kill tumor cells. Further provided are preparation and identification methods for the antibody as well as a biomarker for the administration of such antibody. The present invention has good application prospects in the aspect of preparation of an anti-tumor drug.

The application discloses a monoclonal antibody of a porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody can specifically recognize a linear B cell epitope on the VP6 protein, and a core sequence of the epitope is TTRLSFQLVR. The antibody has high affinity and high specificity, and can cross-react with porcine rotaviruses of various genotypes such as G5, G9 and G11. The application further includes an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in detection of porcine rotaviruses, development of vaccines and research on functions of viral proteins. The application provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of porcine rotaviruses.

The invention relates to the technical field of riemerella anatipestifer detection, and provides a biomarker and a detection method for detecting riemerella anatipestifer, and the biomarker is a host mucosal lesion product generated by induction of specific virulence factors of riemerella anatipestifer after duck infection. Comprising a mucoprotein cracking fragment containing a linear epitope GTTPSPVP, a sialylated sugar chain containing a specific sialic acid modification motif, and a Muc2 degradation peptide fragment. The detection method comprises the following steps: collecting a sample, pretreating, detecting corresponding markers for excrement, respiratory tract lavage fluid and mucous membrane scrape samples by adopting ELISA (Enzyme-Linked Immunosorbent Assay), an immunochromatography test strip and a lectin chip respectively, and judging a result according to a set threshold value. The kit is high in specificity, covers the whole infection period, can realize early and on-site rapid detection, and improves the diagnosis reliability.

The application discloses a method for reducing the allergenicity of tenebrio molitor by ultrasonic and enzymatic hydrolysis, and relates to the technical field of food processing and food safety. First, the tenebrio molitor protein is extracted by an alkali solution method, and then the tenebrio molitor protein solution is treated by ultrasonic and enzymatic hydrolysis to change the conformational epitope and linear epitope of the tenebrio molitor protein, and finally, the treated tenebrio molitor protein solution is freeze-dried to obtain tenebrio molitor protein powder with low allergenicity. The ultrasonic cavitation effect and the specific hydrolysis of enzymatic hydrolysis are used to produce a synergistic effect, and under the combined treatment of 300 W ultrasonic power and alkaline protease, the allergenicity reduction rate can reach more than 70%, which is significantly better than single processing mode, and provides technical support for the development of tenebrio molitor low allergenic food.

The invention belongs to the technical field of biological medicine, and provides an anti-cryptococcus antigen-antibody pair and application thereof in a chemiluminescence method kit. The anti-cryptococcus antigen and antibody pair comprises an anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-2H12 and an anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-4B09; the anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-2H12 has a light chain variable region of which the amino acid sequence is as shown in SEQ ID NO.1 and a heavy chain variable region of which the amino acid sequence is as shown in SEQ ID NO.2; the anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-4B09 has a light chain variable region of which the amino acid sequence is as shown in SEQ ID NO.3 and a heavy chain variable region of which the amino acid sequence is as shown in SEQ ID NO.4. According to the present invention, the anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-2H12 is combined with the conformation-dependent epitope of the beta-1, 2-xylose side chain, the anti-cryptococcus capsular polysaccharide monoclonal antibody GMX-4B09 recognizes the O-acetylation modified linear epitope, and the detection blind area caused by antigen oligomerization is effectively avoided through the double-epitope sandwich method design;

Anti-insect CDA nanobodies are provided. Accordingly, there is provided a nanobody which specifically binds to a non-linear epitope of insect CDA, said non-linear epitope is in the catalytic and / or chitin binding domain of the insect CDA, wherein binding of said nanobody to said insect CDA confers an insect control activity to said nanobody. Also provided are anti-insect CDA nanobodies defined by specific CDR sequences. Also provided are polynucleotides encoding the nanobodies, host cells expressing the nanobodies and methods of using them.

To provide a screening method of polypeptides derived from glycosylated proteins and a polypeptide vaccine combination, having an effective and strong specific anti-tumor immune response effect.SOLUTION: The method comprises: a step 1 of screening N-mannan oligosaccharide-modified proteins and modification sites; a step 2 of identifying a sequence of the modified proteins, cellular localization, immunogenic regions, conservation of glycosylation modification sites, expression levels in tissue cells, site mutations, and effect thereof on protein stability; a step 3 of identifying conservation of residue expression of the modification sites; a step 4 of predicting antigen polypeptides having high HLA affinity and B cell linear epitopes from polypeptides containing the modification sites; and a step 5 of scanning the modification sites of the antigen polypeptides, simulating binding between HLA-A*24:02 molecules and the antigen polypeptides, and identifying parameters of binding stability.SELECTED DRAWING: None

The invention discloses a neutralizing monoclonal antibody aiming at a pig delta coronavirus S1 protein conserved linear epitope and application of the neutralizing monoclonal antibody, and belongs to the technical field of biology. The amino acid sequence of a light chain of the neutralizing monoclonal antibody is as shown in SEQ ID NO.9, and the amino acid sequence of a heavy chain of the neutralizing monoclonal antibody is as shown in SEQ ID NO.13. According to the invention, an insect baculovirus expression system is utilized to express the S1 protein of the porcine delta coronavirus and screen out a linear epitope with a neutralizing effect, and a neutralizing monoclonal antibody is harvested. The monoclonal antibody can be subjected to neutralization reaction with multiple strains of porcine delta coronaviruses, and is strong in neutralization activity and high in titer. The neutralizing monoclonal antibody provided by the invention can be used for efficiently preventing and treating the porcine delta coronavirus. The invention provides a new way for diagnosing, preventing and treating the porcine delta coronavirus, and provides a theoretical basis for development of related medicines and vaccines.

A specific immunogenic peptide for the production of rabbit antibody against human complement protein C4d. In transplants, rejection of the donor organ by the recipient may occur. Immunohistochemical examination can detect pathological changes in the transplant after its rejection. A part of the tissue is examined for the presence of the C4d component of complement as a marker of organ rejection. A unique immunogenic peptide was designed: CAQETGDNLYWGSVTGSQSNAVSP according the C4 complement protein sequence (P0C0L4 • CO4A_HUMAN), including the C4d fragment sequence. It is located in the interval of 1221 to 1243 amino acids in the original sequence and is part of the alpha chain of the complement C4 protein. The immunogenic peptide contains the selected linear epitope GSVTG for the antibody against C4d in the range of 1231 to 1235 amino acids. Anti-C4d antibody is applicable in clinical diagnosis of organ rejection after transplants.

This invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein, comprising: a PTGS1 capture antibody, an unlabeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a blocking buffer, a washing buffer, and a stop solution; the IgG secondary antibody specifically binds to the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody that specifically binds to the linear epitope A of human PTGS1 protein, the amino acid sequence of which is SEQ ID NO:1, corresponding to amino acids 271-282 of human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody that specifically binds to the B epitope of human PTGS1 protein; the amino acid sequences of the B epitope at the A epitope do not overlap and do not constitute steric hindrance. This kit avoids the conserved homologous region between PTGS1 and its homologous protein PTGS2, ensuring the accuracy of the capture antibody recognition, and uses an indirect detection mode with enzyme-labeled goat anti-mouse IgG secondary antibody to achieve signal cascade amplification, significantly improving detection sensitivity.

The invention discloses a preparation and quality control method of an A beta monomer. The method comprises the following steps: (1) mixing Abeta polypeptide with a buffer solution to obtain an Abeta polypeptide solution, and separating different components in the Abeta polypeptide solution according to the molecular weight of the polypeptide by using a molecular sieve method; (2) performing immunoblotting analysis on different components obtained in the step (1), and quantitatively analyzing the purity of an A beta monomer in each component by using an antibody for identifying the linear epitope of the A beta polypeptide; (3) testing and monitoring the aggregation kinetics result of the A beta monomer in each component by using sulfo-sulfoline T; and (4) according to the results of the step (2) and the step (3), carrying out quality control on the A beta monomer component. In order to better prepare the A beta monomer, the invention combines multiple protein purification and analysis technologies to solve the problem that A beta oligomer and truncated peptide are mixed in the A beta monomer, and provides a method for preparing the A beta monomer and controlling the quality of the A beta monomer.

The application provides a monoclonal antibody of canine distemper virus and application, and belongs to the animal medical detection technical field.The antibody provided by the application has super-high binding affinity to CDV H protein of the canine distemper virus, and the linear epitope is the 373-381th position of the CDV H protein.The kit of the application adopts nitrocellulose membrane to coat the canine virus specific antibody and a fluorescent marker, and can rapidly detect the virus in the canine sample.The antibody is prepared by a genetic engineering means, and the detection sensitivity is significantly better than that of a conventional colloidal gold test strip and an ELISA detection method.The antibody of the application maintains excellent stability in a high-temperature environment, and shows extremely low cross-reaction characteristics to CAV and other pathogens.Coupled with a special fluorescent detection device, the technology can complete efficient detection of the canine distemper virus in a short time, and is suitable for clinical diagnosis and treatment and on-site rapid screening scenes.

The application provides a method for identifying neutralizing antigen epitopes in bovine coronavirus spike protein, immunogens, compositions and uses, relates to the technical field of biology, and specifically constructs an efficient neutralizing epitope identification route: online prediction, nanoparticle multivalent display, animal immunization, combined evaluation of binding and neutralization, which can screen out epitopes with neutralizing activity from candidate linear epitopes in a relatively short period of time; the multivalent presentation of ferritin nanoparticles significantly improves the detectability of the immune response of weak immunogenic linear peptides; the obtained epitopes can directly serve the development of subunit vaccines, multi-epitope nanovaccines and in vitro diagnostic reagents.

The invention provides a method for identifying a neutralizing epitope of bovine coronavirus spike protein, an immunogen, a composition and application, and relates to the technical field of biology, the method specifically constructs an efficient neutralizing epitope identification route: on-line prediction, nanoparticle multivalent display, animal immunization, and combined evaluation of associativity and neutrality. Epitopes with neutralizing activity can be screened out from candidate linear epitopes in a short period; the multivalent presentation of the ferritin nanoparticles significantly improves the immune response detectability of the weak immunogenicity linear peptide fragment; the obtained epitope can be directly used for development of subunit vaccines, multi-epitope nano vaccines and in-vitro diagnostic reagents.

The application belongs to the technical field of biology and relates to a broad-spectrum monoclonal antibody for recognizing a linear antigen epitope at the C-terminal of foot-and-mouth disease virus VP2 protein and application thereof. The monoclonal antibody pOTB-1 is successfully constructed, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 6, and the amino acid sequence of the light chain variable region is as shown in SEQ ID No. 7. The biological activity of the antibody is verified by indirect immunofluorescence test, enzyme-linked immunosorbent assay and Western-blot. The antibody exhibits broad-spectrum reactivity to O type and A type FMDV and has strong binding affinity. The antibody recognizes a continuous linear B cell epitope at the C-terminal of the structural protein VP2. The epitope sequence is highly conserved among FMDV O type, A type and Asia1 type, which indicates that there is an inter-type common antigen site in the capsid protein VP2. A competitive ELISA detection method is established based on biotinylated pOTB-1, which provides a tool for the prevention and control of FMDV and serological detection, and lays a foundation for the design of FMDV marker vaccine and the evaluation of immunization effect.