12 results about "Linear epitope" patented technology

The application provides preparation and application of a diagnostic monoclonal antibody for a new epitope of human neutrophil lipocalin (HNL). Two new HNL protein conformation epitopes and one new HNL linear epitope are designed and provided, and an anti-HNL antibody with higher binding affinity and better binding specificity is screened to combine the new epitopes. The anti-HNL antibody of the application can be used for preparation of an HNL detection reagent and kit, and has higher sensitivity and specificity when used for detecting HNL protein in a sample, and can be applied to distinguishing bacterial infection and viral infection.

The application discloses a monoclonal antibody of a porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody. The monoclonal antibody can specifically recognize a linear B cell epitope on the VP6 protein, and a core sequence of the epitope is TTRLSFQLVR. The antibody has high affinity and high specificity, and can cross-react with porcine rotaviruses of various genotypes such as G5, G9 and G11. The application further includes an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in detection of porcine rotaviruses, development of vaccines and research on functions of viral proteins. The application provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of porcine rotaviruses.

The application discloses a method for reducing the allergenicity of tenebrio molitor by ultrasonic and enzymatic hydrolysis, and relates to the technical field of food processing and food safety. First, the tenebrio molitor protein is extracted by an alkali solution method, and then the tenebrio molitor protein solution is treated by ultrasonic and enzymatic hydrolysis to change the conformational epitope and linear epitope of the tenebrio molitor protein, and finally, the treated tenebrio molitor protein solution is freeze-dried to obtain tenebrio molitor protein powder with low allergenicity. The ultrasonic cavitation effect and the specific hydrolysis of enzymatic hydrolysis are used to produce a synergistic effect, and under the combined treatment of 300 W ultrasonic power and alkaline protease, the allergenicity reduction rate can reach more than 70%, which is significantly better than single processing mode, and provides technical support for the development of tenebrio molitor low allergenic food.

This invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein, comprising: a PTGS1 capture antibody, an unlabeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a blocking buffer, a washing buffer, and a stop solution; the IgG secondary antibody specifically binds to the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody that specifically binds to the linear epitope A of human PTGS1 protein, the amino acid sequence of which is SEQ ID NO:1, corresponding to amino acids 271-282 of human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody that specifically binds to the B epitope of human PTGS1 protein; the amino acid sequences of the B epitope at the A epitope do not overlap and do not constitute steric hindrance. This kit avoids the conserved homologous region between PTGS1 and its homologous protein PTGS2, ensuring the accuracy of the capture antibody recognition, and uses an indirect detection mode with enzyme-labeled goat anti-mouse IgG secondary antibody to achieve signal cascade amplification, significantly improving detection sensitivity.

The application provides a monoclonal antibody of canine distemper virus and application, and belongs to the animal medical detection technical field.The antibody provided by the application has super-high binding affinity to CDV H protein of the canine distemper virus, and the linear epitope is the 373-381th position of the CDV H protein.The kit of the application adopts nitrocellulose membrane to coat the canine virus specific antibody and a fluorescent marker, and can rapidly detect the virus in the canine sample.The antibody is prepared by a genetic engineering means, and the detection sensitivity is significantly better than that of a conventional colloidal gold test strip and an ELISA detection method.The antibody of the application maintains excellent stability in a high-temperature environment, and shows extremely low cross-reaction characteristics to CAV and other pathogens.Coupled with a special fluorescent detection device, the technology can complete efficient detection of the canine distemper virus in a short time, and is suitable for clinical diagnosis and treatment and on-site rapid screening scenes.

The application provides a method for identifying neutralizing antigen epitopes in bovine coronavirus spike protein, immunogens, compositions and uses, relates to the technical field of biology, and specifically constructs an efficient neutralizing epitope identification route: online prediction, nanoparticle multivalent display, animal immunization, combined evaluation of binding and neutralization, which can screen out epitopes with neutralizing activity from candidate linear epitopes in a relatively short period of time; the multivalent presentation of ferritin nanoparticles significantly improves the detectability of the immune response of weak immunogenic linear peptides; the obtained epitopes can directly serve the development of subunit vaccines, multi-epitope nanovaccines and in vitro diagnostic reagents.

The application belongs to the technical field of biology and relates to a broad-spectrum monoclonal antibody for recognizing a linear antigen epitope at the C-terminal of foot-and-mouth disease virus VP2 protein and application thereof. The monoclonal antibody pOTB-1 is successfully constructed, the amino acid sequence of the heavy chain variable region is as shown in SEQ ID No. 6, and the amino acid sequence of the light chain variable region is as shown in SEQ ID No. 7. The biological activity of the antibody is verified by indirect immunofluorescence test, enzyme-linked immunosorbent assay and Western-blot. The antibody exhibits broad-spectrum reactivity to O type and A type FMDV and has strong binding affinity. The antibody recognizes a continuous linear B cell epitope at the C-terminal of the structural protein VP2. The epitope sequence is highly conserved among FMDV O type, A type and Asia1 type, which indicates that there is an inter-type common antigen site in the capsid protein VP2. A competitive ELISA detection method is established based on biotinylated pOTB-1, which provides a tool for the prevention and control of FMDV and serological detection, and lays a foundation for the design of FMDV marker vaccine and the evaluation of immunization effect.

The invention relates to a double-antibody sandwich ELISA kit for detecting serum PTGS1 protein. The double-antibody sandwich ELISA kit comprises a PTGS1 capture antibody, a non-labeled PTGS1 detection antibody, an enzyme-labeled IgG secondary antibody, a solid-phase carrier, an enzyme substrate, a confining liquid, a washing liquid and a stop solution, the IgG secondary antibody can be specifically combined with the PTGS1 detection antibody; the PTGS1 capture antibody is a rabbit monoclonal antibody specifically combined with a human PTGS1 protein A linear epitope, the amino acid sequence of the A linear epitope is SEQ ID NO: 1, and the A linear epitope corresponds to the 271th to 282th amino acids of the human PTGS1 protein; the PTGS1 detection antibody is a mouse monoclonal antibody which is specifically combined with a human PTGS1 protein B epitope; and the amino acid sequence of the B epitope at the A epitope is not overlapped and does not form steric hindrance. According to the kit, conserved homologous segments of PTGS1 and homologous protein PTGS2 are avoided, the recognition accuracy of a captured antibody is ensured, signal cascade amplification is realized by adopting an indirect detection mode of an enzyme-labeled goat anti-mouse IgG secondary antibody, and the detection sensitivity is remarkably improved.

The invention discloses a monoclonal antibody of porcine rotavirus VP6 protein, an epitope of the monoclonal antibody and application of the monoclonal antibody, the monoclonal antibody can specifically recognize linear B cell epitopes on the VP6 protein, and the epitope core sequence of the monoclonal antibody is TTRLSFQLVR. The antibody has high affinity and high specificity, and can be subjected to cross reaction with various genotypes of porcine rotaviruses such as G5, G9, G11 and the like. The invention also comprises an indirect ELISA detection kit containing the monoclonal antibody, and application of the linear epitope in porcine rotavirus detection, vaccine research and development and virus protein function research. The invention provides an important tool and theoretical basis for serological diagnosis, vaccine evaluation and etiological research of the porcine rotavirus.

The invention provides preparation and application of a monoclonal antibody for diagnosis aiming at a human neutrophil lipocalin (HNL) neoepitope. Two new HNL protein conformational epitopes and a new HNL linear epitope are designed and provided, and the anti-HNL antibody which is combined with the new epitopes and has higher binding affinity and better binding specificity is obtained through screening. The anti-HNL antibody disclosed by the invention can be used for preparing an HNL detection reagent and a kit, has higher sensitivity and specificity when being used for detecting HNL protein in a sample, and can be applied to distinguishing bacterial infection and virus infection.

The present application provides a kind of polypeptide, polypeptide composition, kit and related application.The polypeptide is the polypeptide shown in SEQ ID NO:1 to SEQ ID NO:85, can be specifically bound as follows target antibody: human SNRPC / U1C antibody, human PCNA antibody, human CENPB antibody, human NUP210 / gp210 antibody, human FTCD / 58K golgi protein antibody and GAD antibody.The above-mentioned multiple specific binding target antibody polypeptide or polypeptide set can cover the peptide segment including linear epitope and nonlinear epitope, so that the number of polypeptides used can be reasonably selected according to the number of target antibodies to be detected, which can improve the detection sensitivity and specificity of each polypeptide, improve the detection accuracy, and improve the throughput and efficiency of the detected antibody.

The application belongs to the technical field of immunology and in vitro diagnosis, and relates to a monoclonal antibody capable of specifically recognizing bluetongue virus VP5 protein. A BTV-1 recombinant VP5 protein with N-terminal 1-79 amino acids deleted is expressed; after the protein is used to immunize mice, a monoclonal antibody 5H11 against the BTV-1 VP5 protein is screened, and the heavy chain and light chain variable region sequences coding the antibody are obtained; the monoclonal antibody 5H11 can specifically react with the BTV-1 VP5 protein, and can specifically recognize the natural VP5 protein in the BTV-1 infected cells; through twice truncation expression of the BTV-1 VP5 protein, a B cell linear epitope recognized by the 5H11 on the VP5 protein is identified, which lays a foundation for in-depth study on the structure and function of the BTV VP5 protein and development of a novel vaccine.