Hybridoma cell strain of monoclonal antibody resisting porcine delta coronavirus N protein epitope, antibody secreted by hybridoma cell strain and application of monoclonal antibody

Abstract

The invention belongs to the technical field of biology, and relates to a hybridoma cell strain and an antibody secreted by the hybridoma cell strain, in particular to the hybridoma cell strain of a monoclonal antibody resisting porcine delta coronavirus (PDCoV) N protein epitope, the antibody secreted by the hybridoma cell strain and application of the monoclonal antibody. The amino acid sequence of the antigen epitope is 326-QDWEWDDA-333, the hybridoma cell strain is classified and named as hybridoma cell strain PDCoV-N-6B7, the preservation number is CCTCC NO: C202178, the preservation date is 2021.3. 26, and the preservation address is Wuhan University, Wuhan, China. The antigen epitope of the 6B7 monoclonal antibody is high in conservative property, is a linear epitope, belongs to an immunodominant epitope, and is beneficial to antibody combination in serum to be detected, so that establishment of a blocking ELISA kit by using the monoclonal antibody aiming at the epitope is more sensitive.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a hybridoma cell strain and an antibody secreted thereto, in particular to a hybridoma cell strain and an antibody secreted by a monoclonal antibody against porcine delta coronavirus N protein antigen epitope and application thereof. Background technique [0002] Porcine deltacoronavirus (PDCoV), a member of the genus Deltacoronavirus in the Coronaviridae family, is a new enteric coronavirus that has emerged in recent years and can cause acute diarrhea, vomiting, dehydration, atrophy of intestinal villi, and even death in suckling piglets. Seriously affect the growth of pigs and cause serious harm to the pig industry. DCoV causes piglet mortality as high as 30%-50%, and currently there is no effective vaccine or treatment. PDCoV was first identified and reported by Woo et al. in 2012, and then PDCoV spread rapidly to many countries, with a potential trend of global epidemic, which has...

AI Technical Summary

Problems solved by technology

The current ELISA methods for detecting antibodies to PDCoV N protein are all indirect methods, but current research has found that there is a certain cross-over between the N proteins of different coronaviruses, and their serum may cross-react with other coronaviruses. In order to solve the existing PDCoV N To solve the problems in the detection of protein antibodies, it is urgent to establish a detection method based on the N protein epitope and its paired monoclonal antibody to achieve rapid detection of PDCoV

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