Antiviral serotype shared monoclonal antibody of foot-and-mouth disease and distinguished epitope thereof

A monoclonal antibody, non-serotype technology, applied in the direction of antiviral agents, antiviral immunoglobulin, antibodies, etc., can solve the problem that there are no reports of shared epitope monoclonal antibodies, no research reports, and the inability to accurately determine the epitope. The position and nature of the bits, etc.

Inactive Publication Date: 2010-02-10
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology has been developed by combining specific types of molecules from different sources into one type that can be used with certain diseases or conditions like feline leukemia virus (FLD). These specialized proteins help identify these viruses more easily than other methods such as serology testing.

Problems solved by technology

This patented technical problem addressed in the patents relating to the development of effective methods for controlling viruses like Foot & Mouth Virus Variants (Human Pathogens), including those transmitted through insect vectors such as mosquitos, bats, rodents, horses, domesticated livestocks, etc., particularly when they occur together during international travel between developed areas where humans live without protection measures. Current vaccines used alone may provide limited effectiveness due to their ability to stimulate specific parts of host cells rather than specifically targets them.

Method used

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  • Antiviral serotype shared monoclonal antibody of foot-and-mouth disease and distinguished epitope thereof
  • Antiviral serotype shared monoclonal antibody of foot-and-mouth disease and distinguished epitope thereof
  • Antiviral serotype shared monoclonal antibody of foot-and-mouth disease and distinguished epitope thereof

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preparation example Construction

[0041] 3. Mouse Immunization and Preparation of Monoclonal Antibody

[0042](1) The O-type FMDV cell-adapted virus is inoculated on BHK-21 cells that are overgrown with a single layer, and the virus is harvested after more than 75% of the cells have pathological changes. The harvested cell culture medium was repeatedly frozen and thawed three times to initially lyse the cells and release the virus. The frozen-thawed virus solution was added to TritonX-100 at 1:1000 and formaldehyde at 4:10000 to lyse and inactivate for more than 48 hours, and 1 mL of the inactivated virus solution was taken to BHK-21 cells for three generations to test whether the inactivation was complete. Centrifuge the freeze-thawed culture medium at 9000rpm (Beckman high-speed centrifuge, JA-10 rotor) at 4°C for 90min, recover the virus supernatant, and discard the cell pellet. Add 80g / L PEG6000 and 40g / L NaCl according to the volume of the virus liquid supernatant, stir at room temperature for 2h until c...

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Abstract

The invention discloses antiviral serotype shared monoclonal antibody of foot-and-mouth disease and a distinguished epitope thereof and the application, belonging to the prevention and control field of foot-and-mouth disease. The invention obtains two hybridoma cell lines which can secrete antiviral serotype shared monoclonal antibody of foot-and-mouth disease (microorganism maintenance numbers thereof are CGMCC No. 3103 and CGMCC No. 3104 respectively), and monoclonal antibodies 4B2 and 3D9 which are secreted by the hybridoma cell lines. ELISA and indirect immunofluorescence assay show that the two hybridoma cell lines both belong to FMDV shared monoclonal antibodies; wherein the 4B2 distinguishes one linear epitope of FMDV, and the 3D9 distinguishes one conformation epitope of FMDV. The4B2 and 3D9 are respectively used as a capture antibody and a detection antibody, a method for capturing ELISA by an antigen is adopted, specificity detection of FMDV can be carried out; the detectionmethod has the characteristics of high sensibility, strong specificity and good repeatability.

Description

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Claims

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Application Information

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Owner HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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