Antiviral serotype shared monoclonal antibody of foot-and-mouth disease and distinguished epitope thereof

Abstract

The invention discloses antiviral serotype shared monoclonal antibody of foot-and-mouth disease and a distinguished epitope thereof and the application, belonging to the prevention and control field of foot-and-mouth disease. The invention obtains two hybridoma cell lines which can secrete antiviral serotype shared monoclonal antibody of foot-and-mouth disease (microorganism maintenance numbers thereof are CGMCC No. 3103 and CGMCC No. 3104 respectively), and monoclonal antibodies 4B2 and 3D9 which are secreted by the hybridoma cell lines. ELISA and indirect immunofluorescence assay show that the two hybridoma cell lines both belong to FMDV shared monoclonal antibodies; wherein the 4B2 distinguishes one linear epitope of FMDV, and the 3D9 distinguishes one conformation epitope of FMDV. The4B2 and 3D9 are respectively used as a capture antibody and a detection antibody, a method for capturing ELISA by an antigen is adopted, specificity detection of FMDV can be carried out; the detectionmethod has the characteristics of high sensibility, strong specificity and good repeatability.

Description

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AI Technical Summary

Benefits of technology

This patented technology has been developed by combining specific types of molecules from different sources into one type that can be used with certain diseases or conditions like feline leukemia virus (FLD). These specialized proteins help identify these viruses more easily than other methods such as serology testing.

Problems solved by technology

This patented technical problem addressed in the patents relating to the development of effective methods for controlling viruses like Foot & Mouth Virus Variants (Human Pathogens), including those transmitted through insect vectors such as mosquitos, bats, rodents, horses, domesticated livestocks, etc., particularly when they occur together during international travel between developed areas where humans live without protection measures. Current vaccines used alone may provide limited effectiveness due to their ability to stimulate specific parts of host cells rather than specifically targets them.

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