202 results about "Capture antibody" patented technology

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention belongs to the technical field of biological medicine, and particularly relates to a kit for breast cancer diagnosis and application thereof. The kit comprises an elisa plate coated with a captured antibody and an HRP labeled antibody working solution, the capture antibody is a monoclonal antibody T2X-1 of an anti-PSTPIP1 protein; the HRP labeled antibody is a monoclonal antibody Z4Y-2 of an anti-PSTPIP1 protein, and the HRP labeled antibody is a monoclonal antibody Z4Y-2 of an The heavy chain amino acid sequence of the monoclonal antibody T2X-1 is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the monoclonal antibody T2X-1 is as shown in SEQ ID NO.2; the heavy chain amino acid sequence of the monoclonal antibody Z4Y-2 is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody Z4Y-2 is as shown in SEQ ID NO.4. The kit for breast cancer diagnosis provided by the invention can be used as an auxiliary diagnosis means for breast cancer with high expression of PSTPIP1 protein, and has high diagnostic value.

The invention provides a group of monoclonal antibody pairs targeting adenovirus DBP protein, the monoclonal antibody pairs comprise a capture antibody and a detection antibody, each of the two antibodies comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises heavy chain complementarity determining regions HCDR1, HCDR2 and HCDR3, the light chain variable region comprises light chain complementarity determining regions LCDR1, LCDR2 and LCDR3, and the capture antibody and the detection antibody are used for capturing and detecting the adenovirus DBP protein. Wherein the capture antibody is a monoclonal antibody 19E3; and the detection antibody is selected from a monoclonal antibody 96 to 10D8, a monoclonal antibody 95 to 2B7, a monoclonal antibody 96 to 2C10, a monoclonal antibody 96 to 8E10, a monoclonal antibody 96 to 13D5 or a monoclonal antibody 96 to 2B10. The monoclonal antibody pair disclosed by the invention can be used for efficiently detecting various types of HAdV DBP.

The invention provides a method for detecting an external vesicle marker through high-flux nano plasma exciting light immune color development, and belongs to the technical field of human extracellular vesicles. After a serum sample is subjected to centrifugal treatment, the serum sample and a CD81 capture antibody substrate are incubated, and the particle size distribution of the vesicles is monitored in real time; a zwitterionic polymer modified gold nanoparticle LAM detection probe and a polyethylene glycol modified silver nanoparticle LprG detection probe are prepared to be specifically combined with a vesicle surface antigen, a chromogenic enhancement solution is adopted to induce a plasma resonance signal, and full-hole scanning imaging is carried out; and constructing a double-layer game optimization model to cooperatively optimize the detection sensitivity and the signal stability, carrying out weighted summation on normalized signals of the particle size subgroups to obtain comprehensive detection signal intensity, comparing the comprehensive detection signal intensity with a threshold value, and outputting a final judgment result. The technical problem that quantitative accuracy is affected by signal intensity deviation caused by vesicle particle size difference in outer vesicle marker detection is solved.

The invention belongs to the technical field of immunodetection, and discloses a kit for detecting the ratio of proBDNF to maure BDNF based on structural dynamics guidance and a preparation method of the kit, the kit comprises a solid phase carrier and a detection reagent, the three antibodies are determined on the basis of full-length conformational kinetics analysis of brain-derived neurotrophic factors; the first capture antibody targets an innate disorder region (SEQ ID NO: 1) of a proBDNF propeptide region; the second capture antibody targets an innate disorder region (SEQ ID NO: 2) of a proBDNF propeptide region; the second capture antibody is a neo-epitope specific antibody, is specifically combined with an N terminal (SEQ ID NO: 2) exposed by maure BDNF enzyme digestion, and is combined with a free alpha-amino group strictly dependent on the first histidine; and the universal detection antibody is combined to a Loop 4 region (SEQ ID NO: 3) of the Mature structural domain. The problems of steric hindrance and cross reaction in traditional immunodetection are solved through a structural biology strategy, and accurate distinguishing and ratio quantification of proBDNF and maure BDNF are achieved.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.

The invention discloses an immune quantitative detection device for tumor markers, relates to the technical field of medical detection, and aims to solve the problems of complicated operation, long detection period and poor portability in immune quantitative detection of tumor markers in the prior art. Comprising a shell, a disposable detection card, a multi-channel micro-fluidic chip, a high-sensitivity sensing system, a micro magnetic control incubation module, an AI auxiliary image recognition system and a wireless transmission module, the disposable detection card is used for accommodating the multi-channel micro-fluidic chip and a reaction reagent, and the multi-channel micro-fluidic chip is respectively aligned with the high-sensitivity sensing system and the micro magnetic control incubation module; the high-sensitivity sensing system is composed of a surface plasma resonance sensor and a quantum dot fluorescence labeling module, and the surface of a sensing chip of the surface plasma resonance sensor is modified with a tumor marker specific capture antibody. The method has the advantages of convenient detection and high efficiency and precision.

The invention discloses a high-throughput electrochemical detection platform based on an ultra-infiltration micro-droplet array, a preparation method of the high-throughput electrochemical detection platform and application of the high-throughput electrochemical detection platform in AD marker detection, and belongs to the technical field of intersection of analytical chemistry, biosensing and material science. The method comprises the following steps: cleaning and drying a substrate, and immersing the surface of the substrate in a fluorosilane solution to form a super-hydrophobic layer by adopting a dip-coating method; after covering with a mask plate, etching with O2 plasma so as to form a hydrophilic microdot matrix; respectively printing an Ag / AgCl reference electrode, a gold working electrode and a carbon counter electrode on the hydrophilic microdots; electrode wires are printed layer by layer, a reference electrode wire, a working electrode wire and a counter electrode wire are sequentially printed in the area outside the hydrophilic microdot matrix from bottom to top in a spin-coating PDMS layer separation mode after each layer of electrode wire is printed, and PDMS is spin-coated above the counter electrode wire for packaging after printing is completed; and on each gold working electrode, respectively modifying capture antibodies of A beta 42, A beta 40, P-tau181 and P-tau217 proteins through a sample application instrument, so as to obtain the detection kit.

The invention provides a method for detecting an anti-C1 esterase inhibitor neutralizing antibody in a biological sample, and the method mainly utilizes the characteristic that protein A resin is combined with an Fc segment of the antibody to capture the antibody (containing the anti-C1 esterase inhibitor neutralizing antibody), so that the C1 esterase inhibitor and complement component 1s (C1s) in the biological sample after thermal inactivation are removed; furthermore, an antibody (containing a neutralizing antibody) or an anti-C1 esterase inhibitor compound on the protein A resin is subjected to acid dissociation through an acidolysis solution, so that not only can the anti-C1 esterase inhibitor neutralizing antibody existing in a free form in a biological sample be detected, but also the anti-C1 esterase inhibitor neutralizing antibody existing in a combined form in the biological sample can be detected.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in macrophage polarization and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture system is used for preparing a capture antibody coupled with a fluorescence coding microsphere by coupling a biotinylated antibody with a fluorescence microsphere coated with biotin, and the detection system is used for preparing a fluorescence labeled antibody by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody. The mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect multiple cell factors, the sample dosage is only 25 microliters, the kit is suitable for in-vitro tests of mouse mononuclear macrophage leukemia cell lines RAW264.7, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention discloses a protein quantitative detection method which comprises the following steps: respectively constructing a capture probe for capturing antibody coupling magnetic beads and oligonucleotides and a detection probe for detecting antibody coupling oligonucleotides through a biorthogonal chemical coupling method; then, target protein is captured by using a biorthogonal chemical coupling and magnetic bead sandwich method comprising the steps of capture probe combination, closing, washing, detection probe combination and the like, and accurate quantification of low-abundance protein in a complex sample is realized by combining a subsequent digital PCR (Polymerase Chain Reaction) technology.

This invention relates to the field of chemiluminescence immunoassay technology, specifically to a magnetic bead coating method and its application for improving the luminescence level of p-tau 217. This invention optimizes the process of coating p-tau 217 capture antibodies with carboxyl magnetic beads. By gradient adjustment of the input ratio of EDC and NHS, the optimal activator concentration is obtained to enhance the magnetic bead coupling activity, increase the antibody coupling efficiency, and thus improve the signal values ​​of antigen samples and clinical samples. This invention has broad application prospects for the development of highly sensitive detection of low-abundance proteins such as p-tau 217.

The invention provides a double-antibody directionally-coupled FET (Field Effect Transistor) biosensor and a preparation method thereof, and belongs to the technical field of biosensors. The FET biosensor adopts a reference electrode to provide grid voltage, a buffer solution is a conductive electrolyte, the FET biosensor comprises a field effect transistor, a capture antibody and a detection antibody, the field effect transistor is composed of a source electrode, a drain electrode and a channel between the source electrode and the drain electrode, the bottom layer of the channel is an indium oxide thin film layer, the indium oxide thin film layer is modified with amino, and the detection antibody is a detection antibody. An amino-modified channel is obtained; carrying out passivation treatment on the source electrode and the drain electrode by adopting a mercaptan passivator; and cross-linking and fixing the capture antibody and the detection antibody on the channel modified with the amino group through a cross-linking fixing agent to obtain the FET biosensor. The two antibodies are combined according to different epitopes of antigens to form a sandwich structure, and double recognition of the antibodies on target antigens is utilized to enhance antigen capture efficiency and signal enrichment, so that a double-site recognition mode is formed, and low-concentration antigen detection is realized.

The preparation method comprises the following steps: immobilizing a specific capture antibody Ab1 of a carcino-embryonic antigen on the surface of a gold electrode, closing a non-specific site by bovine serum albumin to obtain a BSA / Ab1 / GE working electrode, capturing the carcino-embryonic antigen, fusing the carcino-embryonic antigen with an enzyme-coated HHOF-Ab2 nano probe, and detecting the enzyme-coated HHOF-Ab2 nano probe to obtain the sandwich type electrochemical immunosensor based on the enzyme-coated hydrogen bond organic framework hybrid catalyst. The enzyme coated HOF-based sandwich type electrochemical immunosensor is obtained; the sensor specifically recognizes CEA through 'Ab1-CEA-Ab2' double antibody sandwich, the cyclic reaction of H2O2 and HQ is catalyzed by virtue of the enzyme-coated HOF nanoprobe to amplify a signal, and finally chemical change caused by oxidizing hydroquinone into benzoquinone is converted into quantifiable current by virtue of an electrochemical workstation, so that ultra-sensitive detection of CEA is realized, and the sensor has the advantages of high sensitivity, high response speed, strong specificity and the like.

The present invention addresses the problem that, during measurement using a flow channel device, the formation of a capture antibody–analyte–detection antibody complex becomes insufficient due to high flow velocity of the specimen, resulting in reduction of the sensitivity of examination. In order to solve the problem, provided is a flow channel device for analyzing a test substance, the flow channel device having a flow channel region surrounded by a flow channel wall provided inside a porous substrate, wherein: the flow channel region includes an addition portion for adding a liquid, a ligand portion provided with a ligand, an absorption portion into which the liquid flows, and a connection portion connecting any two selected from the group consisting of the addition portion, the ligand portion, and the absorption portion; the ligand is a substance that specifically binds to the test substance; and any selected from the group consisting of gelatin, PVA, and a PVA block copolymer adheres to at least a part of the inner wall of the flow channel wall.

This invention relates to the field of chemical or physical testing technology, specifically to a method for detecting multiple SNARE-related proteins in children with ADHD. The method includes: identifying the best-matching capture antibody for a target protein; mixing the matching capture antibodies for various target proteins with a blood sample, and detecting the initial response value of the target protein in the mixed blood sample; identifying a combination of target proteins with spectral spillover interference by utilizing the correlation between the initial response values ​​of any two target proteins; determining the compensated response value and reference weight of the interfered protein by using the initial response value of the interfered protein in the target protein combination and the corresponding mean response value in the healthy control group; and obtaining the weighted total response value of all target proteins and the expression characteristics of the SNARE complex based on the compensated response value and reference weight. The technical solution of this invention can comprehensively, accurately, and conveniently reflect the overall functional state of the SNARE protein complex expression.

This invention relates to an alanine aminotransferase (ALT) detection reagent and its preparation method, belonging to the field of medical testing. The ALT detection reagent comprises nanobodies AC1 and AT1 binding to different ALT binding sites. Nanobodies AC1 are biotinylated and immobilized on an avidin ELISA plate as a capture antibody, while nanobodies AT1 serve as the detection antibody. The ALT detection reagent and method prepared based on these nanobodies can meet the needs for rapid and accurate ALT detection.

The invention relates to the technical field of digital PCR detection, in particular to a single-molecule protein detection method based on digital PCR. The detection method comprises the following steps: fusing digital PCR and immune PCR, capturing an antibody through magnetic bead coupling, binding the captured antigen with a detection antibody of a nucleic acid fragment through streptavidin-biotin coupling to form a double-antibody sandwich structure, amplifying a fluorescence signal by using digital PCR amplification, and calculating to obtain the target protein concentration of a sample to be detected. Therefore, quantitative detection of the single-molecule protein is realized, and the detection limit reaches 0.395 fg / ml. The detection method is accurate in quantification and excellent in sensitivity, the detection cost can be greatly reduced, the detection efficiency is improved, the detection process is simplified, and the high requirement for equipment precision is reduced.

The application discloses a preparation method and application of an HPV16 / 18 type E6 / E7 protein multi-target detection test strip, and belongs to the technical field of biological immune detection and analysis. The test strip comprises a back plate, a sample pad, a combination pad, a nitrocellulose membrane and an absorption pad, AIE fluorescent microsphere labeled probes are distributed on the combination pad, and capture antibodies and AIE solution are distributed on the nitrocellulose membrane. The preparation method comprises three steps of preparation of the nitrocellulose membrane, preparation of the combination pad and assembly of the test strip. The test strip can quantitatively detect PV16 E6, HPV16 E7, HPV18 E6 and HPV18 E7 simultaneously, four signal points correspond to each target, multi-target synchronous rapid detection is realized, the linear range is wide, the detection limit is low, the operation is simple and convenient, the cost is low, and the test strip is expected to become a new tool for efficient and reliable on-site screening of cervical cancer and precancerous lesions.

The application discloses a reagent combination for simultaneously detecting multiple low-abundance proteins based on multiple solid-phase PCR and application thereof, and belongs to the technical field of biomedical detection. The reagent combination comprises a template, a primer pair and a detection probe; the template comprises at least one template A and n templates B; the 3' end of the template A is complementary to 5-10 nt bases of the template B; 5-10 nt bases of the 5' end of the template B are complementary to form a hairpin structure; the upstream primer of the primer pair is complementary to part of bases of the 5' end of the template A, and the downstream primer is complementary to part of bases of the 3' end of the template B; n detection probes are arranged for the n templates B, and the detection probe is complementary to the middle region of the template B. The reagent combination is combined with three antibodies (a capture antibody and a detection antibody pair) to perform multiple amplification, the number of required reagents is reduced, the detection efficiency is improved, and the reagent combination has important application prospects for clinical diagnosis and large-scale screening.

The invention belongs to the technical field of biological medicine, and particularly relates to a detection kit for detecting new coronavirus. According to the invention, a new coronavirus NP protein is taken as an immunogen, monoclonal antibodies aiming at specific epitopes of the new coronavirus NP protein are screened, a specific strain is taken as a capture antibody, the other strain is labeled by horse radish peroxidase and taken as a detection antibody, and a double-antibody sandwich ELISA method for detecting the SARS-CoV-2 NP protein is established. Compared with the prior art, the method has the advantages that the detection time is greatly shortened, the detection can be completed in only 6 hours, and a foundation is laid for rapid detection of an SARS-CoV-2 clinical sample.

This invention discloses a method for the specific detection of human S100ββ homodimer using a single monoclonal antibody. This monoclonal antibody exhibits high specificity and high affinity for recognizing the human S100β subunit, and its heavy and light chain variable region amino acid sequences are shown in SEQ ID NO.1 and SEQ ID NO.5. A specific S100ββ homodimer double-antibody sandwich detection method is established by simultaneously using this monoclonal antibody or its antigen-binding fragment as both the capture antibody and the detection antibody, binding to the same epitope sites on both subunits of the S100ββ homodimer. This method achieves a limit of detection of 0.005 ng / mL for S100ββ homodimer, with a detection linear range of 0.005–10 ng / mL. This method is suitable for the specific detection of S100ββ homodimer.

The invention provides a D-dimer detection kit and a preparation method thereof. A chromatographic test strip of the kit comprises a bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad. And the combination pad is coated with a D-dimer detection antibody marked by carboxylated time-resolved fluorescent microspheres. A detection line of the nitrocellulose membrane is coated with a D-dimer capture antibody, and a quality control line of the nitrocellulose membrane is coated with a goat anti-mouse IgG antibody. The D-dimer detection antibody and the D-dimer capture antibody are a pair of monoclonal antibodies for specifically recognizing different epitopes of the D-dimer. According to the kit, the convenience of immunochromatography and the high sensitivity characteristic of the time-resolved fluorescent microspheres are fused, accurate and quantitative detection of the D-dimer is achieved through the synergistic effect of the detection antibody and the capture antibody which specifically recognize different epitopes, and the kit is high in sensitivity, good in specificity, easy and convenient to operate, high in anti-interference capacity and high in sensitivity. The clinical high-precision quantitative monitoring requirement can be met, and the diagnosis and treatment application value and the market prospect are achieved.

The application relates to a preparation method and application of a ternary heterojunction composite photoelectrochemical immunosensor, and belongs to the technical field of photoelectrochemical biosensors, and particularly relates to a preparation method and application of a photoelectrochemical immunosensor for detecting alpha-fetoprotein by adopting a BiPO4 / BiOBr / CdS composite material. The method is characterized in that a ternary heterojunction is assembled on an FTO modified electrode to fix a capture antibody, and the accurate determination of alpha-fetoprotein is realized through a photocurrent signal response. The BiPO4 / BiOBr heterojunction expands the forbidden band width and expands the light absorption range; due to the sensitization of CDs, the absorption of visible light is enhanced, so that the photocurrent signal is increased. The method has the advantages of good stability, strong specificity, high sensitivity, good reproducibility and the like, is good for the rapid detection of alpha-fetoprotein, solves the existing technical difficulties, and provides a feasible method for the detection of tumor markers.

The application discloses a method for constructing a high-throughput protein capture interface by antibody adsorption, which comprises the following steps: S1, synthesizing a tetrahedral framework nucleic acid structure; S2, mixing a capture antibody and the synthesized tetrahedral framework nucleic acid structure on a gold island substrate to prepare a high-throughput protein capture interface; S3, placing the gold island substrate in a wet box for overnight incubation; S4, blocking the capture interface to prevent non-specific adsorption; S5, incubating a protein target; S6, incubating a detection antibody; S7, incubating a fluorescent secondary antibody; S8, washing the capture interface with pure water and centrifuging and air-drying; and S9, performing fluorescent imaging and quantification on the capture interface by using a chip scanner. The method for constructing a high-throughput protein capture interface by antibody adsorption has high uniformity, and can realize high-throughput, high-sensitivity and high-accuracy protein capture and quantitative detection.

The application discloses a method for detecting specific activity of recombinant Pseudomonas aeruginosa vaccine rPO stock solution, and comprises the following steps: (1) using OprI-B052 as a capture antibody to coat an enzyme-labeled plate; (2) adding a sample to be detected into the coated enzyme-labeled plate; (3) diluting an enzyme-labeled antibody PcrV-A039 and then performing a color reaction to measure an OD 450 / 630 nm value; and (4) preparing a standard curve by using a rPO reference product, and calculating the specific activity of the rPO antigen in the sample according to the standard curve. In the application, OprI-B052 is used as a coating antibody, and PcrV-A039 is used as a detection antibody. The specific activity of the recombinant Pseudomonas aeruginosa vaccine rPO stock solution can be quantitatively detected, and the application has good stability and repeatability.

The invention relates to an ELISA (enzyme-linked immuno sorbent assay) kit for quantitative detection of humanized exosomes and a use method of the ELISA kit, the ELISA kit comprises an ELISA plate coated with a capture antibody, a detection antibody solution, an enzyme-labeled streptavidin solution for catalyzing substrate color development and a luminous substrate solution, the capture antibody is an anti-CD81 antibody, and the detection antibody solution is an enzyme-labeled streptavidin solution for catalyzing substrate color development. According to the present invention, the detection antibodies comprise the biotin-labeled anti-CD9 antibody and the biotin-labeled anti-CD63 antibody, the specific capture of the humanized exosome is performed through the anti-CD81 antibody coated on the pore plate, the detection is performed through the anti-CD9 antibody and the anti-CD63 antibody, and the quantification of the humanized exosome is achieved through the enzyme catalysis chemiluminescence principle, by optimizing the elisa plate and each reagent in the kit and adjusting the use method, the humanized exosome is accurately quantified, and the detection result is not interfered by protein impurities, so that the purpose of rapid and accurate quantification is achieved.

The invention belongs to the technical field of disease detection and health examination, and particularly relates to an enzyme-linked immunosorbent assay method of apolipoprotein E and application of the apolipoprotein E in glaucoma diagnosis. A capture antibody used by the kit is 1D4, a heavy chain variable region of the capture antibody comprises HCDR1-3 as shown in SEQ ID NO: 4-6, and a light chain variable region of the capture antibody comprises LCDR1-3 as shown in SEQ ID NO: 1-3; a detection antibody used by the kit is 3A6, a heavy chain variable region of the detection antibody comprises HCDR1-3 as shown in SEQ ID NO: 12-14, and a light chain variable region of the detection antibody comprises LCDR1-3 as shown in SEQ ID NO: 9-11. The double-antibody sandwich ELISA detection kit for detecting ApoE4 provided by the invention has no cross reaction with other subtypes of ApoEs, the used monoclonal antibody has high sensitivity, the kit can be used for screening glaucoma in physical examination and ophthalmic examination of old people, and the screening result is counted and summarized to a health information system and a cloud platform for big data analysis, so that the health condition of a patient can be detected.

The invention discloses a lateral flow chromatography test strip for immunosignal replacement based on electrochemical detection as well as a preparation method and application of the lateral flow chromatography test strip. The test strip comprises a sample pad, a capture antibody pad, a solid-phase protein film, a detection electrode and a signal amplification pad, a capture antibody with a label is fixed on the capture antibody pad and can be combined with an antigen in a sample to form an antigen-antibody compound; solid-phase protein is fixed on the solid-phase protein film and can be combined with an antigen in an antigen-antibody compound; and a target object which can be combined with a label for capturing an antibody is fixed on the signal amplification pad. According to the invention, through the replacement signal of the immune lateral flow of the antigen and the antibody, the antibody which can reflect the antigen amount and is provided with the marker generates an electric signal at the tail end of the lateral flow or is catalyzed and amplified by the marker to generate a strong electrochemical signal, so that the detection accuracy and sensitivity can be obviously improved; and interference signals of electrochemical detection based on immunoreaction are obviously reduced.