514 results about "Capture antibody" patented technology

The invention relates to the technical field of biomedicine, in particular to an anti-influenza A virus NP protein antibody pair and application thereof. The antibody pair comprises two monoclonal antibodies; the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; one monoclonal antibody in the monoclonal antibody pair is named as a 10G10 antibody, and the other monoclonal antibody is named as a 11F8 antibody. The invention also discloses a nucleic acid molecule for coding the monoclonal antibody and a hybridoma cell strain for secreting the monoclonal antibody. In addition, the invention also discloses a kit for detecting influenza virus protein, which comprises the monoclonal antibody pair, the 10G10 antibody is used as a capture antibody, and the 11F8 antibody is used as a labeled antibody. The antibody for resisting the influenza A virus NP protein provided by the invention has relatively good specificity on the influenza virus protein.

Provided are an apolipoprotein E detection reagent and the use thereof. The reagent comprises at least one of an ApoE2 protein detection reagent, an ApoE3 protein detection reagent and an ApoE4 protein detection reagent. One of a capture antibody and a detection antibody used for detecting ApoE2 protein or ApoE4 protein is a specific monoclonal antibody, and the capture antibody and the detection antibody used for detecting ApoE3 are both specific monoclonal antibodies. Genotyping of ApoE and quantitative detection of different genotypes of proteins are performed on the basis of an immunological detection method. The detection method has the advantages of being simple, easy to operate, short in time and cheap. A genotype detection result is highly consistent with a fluorescence PCR result. Genotyping of ApoE (6 types) can be performed, which is used to replace the nucleic acid detection and guide medication, and clarify the correlation of the ApoE4 genotype homozygosity / heterozygosity and the protein concentration of ApoE4 with AD.

The invention discloses a control system and method for multi-channel treatment of a biological sample. A capture antibody layer of an optical fiber SPR sensor and a real-time detection biological solution can be prepared; comprising a microfluidic module, a sensing module and a computer, the sensing module is used for monitoring resonance wavelength information of a biological solution flowing through a sensing area of the optical fiber SPR sensor in real time; the micro-fluidic module is used for enabling the solution in the corresponding liquid storage tank to flow through the outer surface of the optical fiber SPR sensor in the micro-fluidic chip through the micro-fluidic channel under the set pressure; and the computer is used for detecting the corresponding content of the biological solution in the current liquid storage tank by measuring the resonant wavelength and comparing the resonant wavelength with pre-stored data based on the received electric signal of the spectrum. According to the control system, a micro-fluidic chip is used as a container for accommodating a sample to be detected or prepared, and a biological solution slowly flows into the micro-fluidic chip through a micro-fluid channel so as to realize preparation of a capture antibody layer or real-time detection of the biological solution.

The invention relates to the technical field of biological detection, and provides an inflammatory factor detection kit based on a liquid-based chip technology, and the inflammatory factor detection kit comprises six fluorescent microspheres coupled with capture antibodies, and six detection antibodies labeled by report fluorescence; in the six fluorescent microspheres containing the captured antibodies, the fluorescence intensities of all the fluorescent microspheres are different; according to the detection kit provided by the invention, on the basis of a liquid-based chip technology, fluorescein is adopted to carry out fluorescence coding on microspheres, and a captured antibody is connected to the fluorescent microspheres through a chemical reaction; a detection target is captured through the fluorescent microspheres, and then a signal is displayed through the detection antibody, so that the detection of the inflammatory factors is completed, the defects of large demand quantity of soluble inflammatory factor detection samples, long detection time, tedious detection steps and the like in the prior art are overcome, and great convenience is brought to scientific researchers at a hospital end and an industrial end.

The invention belongs to the field of biological detection, and discloses a kit for detecting cervical cancer and application of the kit. The kit comprises an elisa plate coated with a captured antibody, an HRP labeled antibody, an antigen standard substance, a washing solution, a developing solution and a stop solution, the heavy chain amino acid sequence of the monoclonal antibody CPT1A-1 of the anti-CPT1A protein in the capture antibody is as shown in SEQ ID NO. 1, and the light chain amino acid sequence is as shown in SEQ ID NO. 2; the heavy chain amino acid sequence of the monoclonal antibody CPT1A-2 of the anti-CPT1A protein in the HRP labeled antibody is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody CPT1A-2 of the anti-CPT1A protein in the HRP labeled antibody is as shown in SEQ ID NO.4. The kit for diagnosis of cervical cancer provided by the invention has strong specificity, can specifically detect the content of CPT1A protein in serum, has good stability, and has good application prospects.

The invention provides a kit and a detection method for detecting Alzheimer disease related proteins in human blood. The kit comprises a microsphere solution coupled with a p-Tau-217 capture antibody, a microsphere solution coupled with a p-Tau-231 capture antibody, a fluorescein PE coupling detection antibody solution and a diluent, the diluent is prepared from tris (hydroxymethyl) aminomethane, Triton X-100, sodium chloride and Proclin300; according to the kit provided by the invention, the accuracy of detecting the p-Tau-217 protein and the p-Tau-231 protein is improved by designing the microspheres, specifically detecting the p-Tau-217 protein and the p-Tau-231 protein and using a double-antibody sandwich detection method, clinical detection application is more convenient, and the kit is high in specificity, high in sensitivity, good in repeatability and relatively good in application prospect.

The invention discloses a broad-spectrum monoclonal antibody for resisting porcine epidemic diarrhea virus S protein and application of the broad-spectrum monoclonal antibody. Specifically, firstly, two monoclonal antibodies GC33D8-1 and GC33A4-3 which have broad-spectrum binding characteristics with the porcine epidemic diarrhea virus S protein are obtained, the GC33D8-1 antibody has good universality and can have strong affinity with different strains, and the GC33A4-3 antibody can be bound for 3 times. Secondly, the two monoclonal antibodies are respectively used as a capture antibody and a detection antibody, a double-antibody sandwich ELISA method is established, and the double-antibody sandwich ELISA method is applied to PEDV vaccine S protein quantification, has better parallelism compared with other antibodies, and can be widely applied to detection of different strains. The method can be used for detecting semi-finished products and finished products, can be used for process establishment and optimization of porcine epidemic diarrhea virus vaccine antigens, vaccine quality evaluation and stability monitoring, has the advantages of high sensitivity, high accuracy, high repeatability and high flux, and solves the problems that broad-spectrum detection cannot be carried out at present and an S protein ELISA method only can be used for qualitative judgment.

The invention belongs to the technical field of biology, and particularly relates to a group of paired monoclonal antibodies for resisting H9N2 subtype avian influenza viruses and a surface enhanced Raman spectroscopy rapid detection test strip established based on the antibodies. The paired monoclonal antibody comprises a labeled antibody 1E9 and a capture antibody 3C10 which are prepared by taking hemagglutinin protein of the H9N2 subtype avian influenza virus as antigens and can be specifically combined with the H9N2 subtype avian influenza virus. According to the invention, an SERS (Surface Enhanced Raman Scattering) probe Au-coated Pt-4ATP is used as a marker and is coupled with a labeled antibody 1E9 to prepare a labeled probe, and the labeled probe is paired with a capture antibody 3C10 to establish an SERS immunochromatography test strip. The test strip has a colorimetric and Raman dual interpretation mode, can be used for qualitative and quantitative detection, has the advantages of strong specificity, high sensitivity, good repeatability and stability and the like, is simple and rapid in clinical detection operation, and provides reliable technical support for on-site rapid screening of subtype viruses.

The invention discloses a high-sensitivity cardiac troponin-T electrochemiluminescence detection kit. The kit comprises a magnetic microsphere reagent coated by a high-sensitivity cardiac troponin-T capture antibody and a high-sensitivity cardiac troponin-T detection antibody reagent labeled by an electrically neutral ruthenium compound, the structural general formula of the electrically neutral ruthenium compound is # imgabs0 # n = 0-9; the magnetic microsphere reagent coated with the high-sensitivity cardiac troponin-T capture antibody comprises a magnetic microsphere coated with the high-sensitivity cardiac troponin-T capture antibody and a diluent; the electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody reagent comprises an electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody and a diluent; the diluent is prepared from piperazine-1, 4-diethyl sulfonic acid or 3-(N-morpholinyl)-2-hydroxy propanesulfonic acid. When the high-sensitivity cardiac troponin-T electrochemiluminescence detection kit is used for detection, a reaction system does not contain streptavidin coated magnetic beads or biotinylated antibodies, so that interference of biotin in a sample is avoided.

The invention provides an immunodetection method of a multi-index immune checkpoint detection kit, which belongs to the technical field of immunodetection and comprises three main stages of sample preparation, immune reaction and data analysis. The method comprises the following steps: firstly, carrying out centrifugal separation to obtain a plasma sample, and preparing a fluorescent microsphere coupled capture antibody and biotin labeled detection antibody compound; then carrying out immune reaction, forming a sandwich type immune complex through specific binding, and adding streptavidin marked by phycoerythrin for signal amplification. Detecting a fluorescence signal by adopting a flow cytometer to obtain an original data matrix; a contribution feature vector, a utility feature vector and an expression feature matrix are extracted through a feature decomposition algorithm, and a deep learning model of a total-score-total structure is established for data analysis. Finally, a detection report containing a quantitative result, an importance score and reliability evaluation is generated, and the technical problem that in the prior art, the analysis accuracy of a multi-index immune checkpoint detection result is insufficient is solved.

Provided herein are methods of detecting target molecules. The methods include contacting a sample comprising the target molecule with a substrate that comprises a plurality of capture antibodies, or antigen binding portions thereof, that specifically bind to the target molecule to form captured target molecules, and contacting the captured target molecules with a plurality of detection antibodies, or antigen binding portions thereof, that bind to the captured target molecules to form target molecule complexes. The methods also include taking images of the target molecule complexes to produce imaged target molecule complexes, and quantifying an amount of target molecules in the sample using the imaged target molecule complexes. Additional methods as well as related devices and systems are also provided.

The invention belongs to the technical field of biological medicine, and particularly relates to a kit for breast cancer diagnosis and application thereof. The kit comprises an elisa plate coated with a captured antibody and an HRP labeled antibody working solution, the capture antibody is a monoclonal antibody T2X-1 of an anti-PSTPIP1 protein; the HRP labeled antibody is a monoclonal antibody Z4Y-2 of an anti-PSTPIP1 protein, and the HRP labeled antibody is a monoclonal antibody Z4Y-2 of an The heavy chain amino acid sequence of the monoclonal antibody T2X-1 is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the monoclonal antibody T2X-1 is as shown in SEQ ID NO.2; the heavy chain amino acid sequence of the monoclonal antibody Z4Y-2 is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody Z4Y-2 is as shown in SEQ ID NO.4. The kit for breast cancer diagnosis provided by the invention can be used as an auxiliary diagnosis means for breast cancer with high expression of PSTPIP1 protein, and has high diagnostic value.

The invention belongs to the technical field of in-vitro diagnosis, and discloses a microbiological detection kit and a detection method. The kit comprises an elisa plate coated with a captured antibody and an HRP labeled antibody working solution, the capture antibody is a monoclonal antibody LG3A-1 specifically combined with a human giardia antigen, the heavy chain amino acid sequence of the capture antibody is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the capture antibody is as shown in SEQ ID NO.2; the HRP labeled antibody is a monoclonal antibody FLSP4 specifically combined with human giardia antigen, the heavy chain amino acid sequence of the monoclonal antibody FLSP4 is as shown in SEQ ID NO.3, and the light chain amino acid sequence of the monoclonal antibody FLSP4 is as shown in SEQ ID NO.4. The microbiological detection kit provided by the invention can be used for detecting the content of giardia in human excrement, is high in specificity, high in sensitivity, good in stability and simple to operate, and has a very good application prospect.

The invention discloses a magnetic particle chemiluminiscence detection kit for human non-phosphorylated Tau217 protein and a preparation method of the magnetic particle chemiluminiscence detection kit. The kit comprises a micro magnetic particle coupled with a human non-phosphorylated Tau217 protein capture antibody, an acridinium ester labeled human non-phosphorylated Tau217 protein detection antibody, a human non-phosphorylated Tau217 protein calibration product and a quality control product. The direct chemiluminescence method established by the invention has the advantages of high sensitivity, strong specificity, accuracy, rapidness and short detection time, the detection result has higher accuracy and repeatability, and the kit can be used for various luminescence detection instruments.

The invention provides a group of monoclonal antibody pairs targeting adenovirus DBP protein, the monoclonal antibody pairs comprise a capture antibody and a detection antibody, each of the two antibodies comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises heavy chain complementarity determining regions HCDR1, HCDR2 and HCDR3, the light chain variable region comprises light chain complementarity determining regions LCDR1, LCDR2 and LCDR3, and the capture antibody and the detection antibody are used for capturing and detecting the adenovirus DBP protein. Wherein the capture antibody is a monoclonal antibody 19E3; and the detection antibody is selected from a monoclonal antibody 96 to 10D8, a monoclonal antibody 95 to 2B7, a monoclonal antibody 96 to 2C10, a monoclonal antibody 96 to 8E10, a monoclonal antibody 96 to 13D5 or a monoclonal antibody 96 to 2B10. The monoclonal antibody pair disclosed by the invention can be used for efficiently detecting various types of HAdV DBP.

The invention provides a method for detecting an external vesicle marker through high-flux nano plasma exciting light immune color development, and belongs to the technical field of human extracellular vesicles. After a serum sample is subjected to centrifugal treatment, the serum sample and a CD81 capture antibody substrate are incubated, and the particle size distribution of the vesicles is monitored in real time; a zwitterionic polymer modified gold nanoparticle LAM detection probe and a polyethylene glycol modified silver nanoparticle LprG detection probe are prepared to be specifically combined with a vesicle surface antigen, a chromogenic enhancement solution is adopted to induce a plasma resonance signal, and full-hole scanning imaging is carried out; and constructing a double-layer game optimization model to cooperatively optimize the detection sensitivity and the signal stability, carrying out weighted summation on normalized signals of the particle size subgroups to obtain comprehensive detection signal intensity, comparing the comprehensive detection signal intensity with a threshold value, and outputting a final judgment result. The technical problem that quantitative accuracy is affected by signal intensity deviation caused by vesicle particle size difference in outer vesicle marker detection is solved.

The invention belongs to the technical field of immunodetection, and discloses a kit for detecting the ratio of proBDNF to maure BDNF based on structural dynamics guidance and a preparation method of the kit, the kit comprises a solid phase carrier and a detection reagent, the three antibodies are determined on the basis of full-length conformational kinetics analysis of brain-derived neurotrophic factors; the first capture antibody targets an innate disorder region (SEQ ID NO: 1) of a proBDNF propeptide region; the second capture antibody targets an innate disorder region (SEQ ID NO: 2) of a proBDNF propeptide region; the second capture antibody is a neo-epitope specific antibody, is specifically combined with an N terminal (SEQ ID NO: 2) exposed by maure BDNF enzyme digestion, and is combined with a free alpha-amino group strictly dependent on the first histidine; and the universal detection antibody is combined to a Loop 4 region (SEQ ID NO: 3) of the Mature structural domain. The problems of steric hindrance and cross reaction in traditional immunodetection are solved through a structural biology strategy, and accurate distinguishing and ratio quantification of proBDNF and maure BDNF are achieved.

The invention belongs to the technical field of biology, and relates to a latex immunochromatography test strip for rapidly detecting porcine epidemic diarrhea virus (PEDV) and a preparation method of the latex immunochromatography test strip. The test strip comprises a PVC (polyvinyl chloride) bottom plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad, a quality control line C and a detection line T are arranged on the nitrocellulose membrane. A latex microsphere labeled PEDV N protein monoclonal capture antibody is sprayed on the combination pad, a detection line T is coated with a PEDV N protein monoclonal detection antibody 1E1, and a quality control line C is coated with goat anti-mouse IgG. The test strip can specifically recognize PEDV, and does not react with other porcine intestinal coronaviruses with similar clinical symptoms. The test strip is good in specificity, relatively high in sensitivity and simple to operate, a result is interpreted within 10 minutes, and compared with a traditional colloidal gold detection technology, the stability and the color development effect are obviously improved.

The invention provides a hybridoma cell strain secreting a grass carp reovirus VP4 protein monoclonal antibody as well as the monoclonal antibody and application thereof, and belongs to the technical field of aquatic reovirus detection. The hybridoma cell strain is named as GCRV-VP4-6E7B12, and is preserved in the China Center for Type Culture Collection on December 10, 2024, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: C2024160. The grass carp reovirus VP4 protein monoclonal antibody 6E7B12 secreted by the hybridoma cell strain GCRV-VP4-6E7B12 has the advantage of being specifically combined with an antigen. The enzyme-labeled monoclonal antibody is used as a detection antibody, the VP4 protein polyclonal antibody is used as a capture antibody, the content of the grass carp reovirus VP4 protein in a sample to be detected can be quantitatively detected by adopting a double-antibody sandwich ELISA method, the detection range is 1-32 ng / mL, and the lower detection limit is 1 ng / mL. The detection method has the advantages of high sensitivity and wide detection range.

The invention belongs to the technical field of in-vitro diagnosis, and particularly relates to a detection kit and a detection method for a pretumor marker. The kit comprises an elisa plate coated with a captured antibody, an HRP labeled antibody, an antigen standard substance, a washing solution, a developing solution and a stop solution, the capture antibody and the HRP labeled antibody are both prepared from a monoclonal antibody of the anti-SLC31A1 protein. According to the detection method of the kit for detecting the pretumor marker, the SLC31A1 protein content in human serum is detected through the kit, the specificity is high, a large batch of samples can be rapidly detected in a high-throughput mode, the cancer risk of a to-be-detected crowd can be effectively judged, and therefore relevant patients can find and treat the pretumor marker in advance, treatment prognosis is improved, and the death rate is reduced.

The invention provides a monoclonal antibody capable of specifically recognizing a specific metabolic fragment of human amyloid protein and application of the monoclonal antibody, and belongs to the technical field of genetic engineering. The invention provides a hybridoma cell strain AB5H, and the preservation number of the hybridoma cell strain AB5H is CCTCC (China Center For Type Culture Collection) NO: C201255. The hybridoma cell strain AB5H disclosed by the invention can secrete a monoclonal antibody which has relatively high activity and can specifically recognize human beta amyloid protein A beta 40; the monoclonal antibody is high in titer, can be used as a capture antibody, and is used for preparing a human Abeta40 protein quantitative detection kit and quantitatively detecting the content of Abeta40 in cerebrospinal fluid and blood, so that the detection result deviation caused by the fact that the content of Abeta40 in peripheral blood is easily influenced by metabolism, sample treatment conditions and methods and individual differences is overcome, and the detection accuracy is improved. The variation degree of a detection result is reduced, so that a judgment boundary value of a blood A beta detection result is conveniently obtained, and AD early diagnosis is realized.

The invention belongs to the technical field of biology, and particularly relates to a kit for detecting breast cancer and a detection method. The kit comprises an elisa plate coated with a capture antibody L4-CAP, a biotin-labeled detection antibody L4-SIG, a protein standard substance, HRP, a sample diluent, a washing solution, a developing solution and a stop solution. The heavy chain amino acid sequence of the capture antibody L4-CAP is as shown in SEQ ID NO.1, and the light chain amino acid sequence of the capture antibody L4-CAP is as shown in SEQ ID NO.2; the heavy chain amino acid sequence of the detection antibody L4-SIG is shown as SEQ ID NO.3, and the light chain amino acid sequence of the detection antibody L4-SIG is shown as SEQ ID NO.4. The kit for detecting the breast cancer is high in specificity, can specifically detect the content of the URCL4 protein in serum, serves as an auxiliary diagnosis means for breast cancer with high expression of URCL4 protein, and has a good application prospect.

The invention discloses a method for quantitatively detecting the specific activity of a MntC antigen in a recombinant staphylococcus aureus vaccine or / and a stock solution. The method comprises the following steps: (1) coating an elisa plate by taking MntC-686 as a capture antibody; (2) adding a sample to be detected into the coated ELISA plate; (3) carrying out a chromogenic reaction by using HRP-MntC-A7, and measuring an OD450 / 630 nm value; and (4) preparing a standard curve by using the MntC reference, and calculating the specific activity of the MntC antigen in the detected sample according to the standard curve. According to the present invention, the fully human monoclonal antibody for specifically recognizing the recombinant staphylococcus aureus vaccine MntC protein is adopted as the coating antibody, and the anti-MntC protein fully human monoclonal antibody is adopted as the detection antibody; the kit can be used for detecting the specificity of the MntC antigen in a staphylococcus aureus vaccine or a stock solution, and has good stability and repeatability.

The invention discloses a kit for auxiliary diagnosis of Alzheimer's disease and a preparation method and application thereof, and relates to the field of medical detection. The kit for auxiliary diagnosis of the Alzheimer's disease comprises a magnetic particle solution coated with a capture antibody, an enzyme-labeled antibody compound, a cleaning solution and a luminous substrate, the magnetic particle solution coated with the capture antibody comprises magnetic particles connected with a first antibody, and the first antibody is specifically combined with a biomarker; the enzyme-labeled antibody compound comprises a second antibody coupled with an enzyme, and the second antibody and the first antibody are specifically combined with the same biomarker; the magnetic particles comprise carboxyl magnetic beads, the carboxyl magnetic beads are coated with Protein A, and the Protein A is connected with a first antibody. By optimizing the coupling process of the antibody, the problems of poor biomarker detection sensitivity and low efficiency of the traditional detection technology are solved, and redundant inspection items for early diagnosis of the Alzheimer's disease and other neurodegenerative diseases are reduced.

The present invention relates to an antibody specific for Staphylococcus aureus protein A, and to a composition or kit comprising same for detecting Staphylococcus aureus or diagnosing skin conditions. The monoclonal antibody according to the present invention is highly reactive to antigens, and, particularly, can detect antigens rapidly and accurately even at low concentrations due to two antibodies of different CDRs having been combined and used as capture and detection antibodies, thus allowing the monoclonal antibody to be used in rapid self-diagnostic kits and the like. In using the monoclonal antibody, the degree of proliferation of Staphylococcus aureus on the skin surface and the like can be visually checked, abnormal states of the skin, such as inflammation and excessive immune response, can be diagnosed, incidence probability and persistence of related diseases can be predicted, and subsequent treatment methods can be determined.

The invention relates to a high-sensitivity immunodetection chip and a method thereof, the chip comprises a sample adding area, the sample adding area is used for introducing a sample to be tested into the chip, and then the sample enters a reagent pre-embedding area; the reagent pre-embedding area is provided with a plurality of detection antibodies with markers; the detection area comprises a plurality of micro-trap structures arranged in an array, each micro-trap structure comprises a plurality of microsphere clamping grooves and flow channels arranged on the peripheries of the microsphere clamping grooves, each microsphere clamping groove captures and fixes a microsphere, each microsphere is coated with a capture antibody, and the capture antibody is coated with an antibody. A to-be-tested sample and a detection antibody pass through the flow channel and then react with the capture antibody on the microspheres to be fixed, the marker forms an array signal in the detection area, and the residual reagent flows into the waste liquid collection area; the waste liquid collecting area is used for collecting liquid flowing out of the detection area. The microsphere array and the detection antibody are pre-fixed, so that the detection process is convenient and rapid, and the detection sensitivity is high.

The invention provides a reagent for detecting avian leukosis virus, a nano-enzyme immunochromatography test strip and application of the nano-enzyme immunochromatography test strip, and belongs to the technical field of immunology. Aiming at the problems that the existing avian leukosis virus detection method is complicated in operation, time-consuming or strong in equipment dependence, the reagent comprises a capture antibody and a detection antibody; a heavy chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 1, and a light chain variable region of the monoclonal antibody A comprises an amino acid sequence as shown in SEQ ID NO: 2; a heavy chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 5, and a light chain variable region of the monoclonal antibody B comprises an amino acid sequence as shown in SEQ ID NO: 6; the antigen binding fragment is a Fab fragment, an F (ab) 2 fragment or a single-chain Fv fragment. The reagent is mainly used for detecting avian leukosis virus, and is suitable for detecting samples such as cloaca swab, meconium, egg white or serum of poultry.

The invention discloses a trypsinogen-2 detection method and kit based on a homogeneous light-excited chemiluminescence immunoassay technology, and belongs to the technical field of immunodetection analysis. The method provided by the invention comprises the following steps: preparing donor microspheres and receptor microspheres coupled with a trypsinogen-2 capture antibody, adding the coupled donor microspheres and receptor microspheres into a sample to be detected, uniformly mixing and incubating without cleaning, and finally detecting by using a fluorescence detector. The kit comprises donor microspheres coupled with a trypsinogen-2 capture antibody, receptor microspheres coupled with a trypsinogen-2 detection antibody, a trypsinogen-2 standard substance solution and an analysis buffer solution. The trypsinogen-2 content in a sample to be detected can be rapidly detected by using the characteristics of a double-antibody sandwich immunoassay and light-activated chemiluminescence, and the trypsinogen-2 detection kit has the characteristics of wide detection range, high sensitivity, high accuracy, rapid and simple detection and the like, can be used for early auxiliary diagnosis of acute pancreatitis clinically, and has a relatively good application prospect.

The invention belongs to the technical field of biological detection, and provides a multiple cell factor detection kit in tumor immunity and a preparation method and application thereof, and the detection kit comprises a capture antibody, a detection antibody, a mixed protein standard substance, a diluent and a washing buffer solution. The capture antibody is prepared by coupling a biotinylated antibody with a fluorescent microsphere coated with biotin to obtain a capture antibody coupled with a fluorescent encoding microsphere, and the detection antibody is prepared by coupling derivatized phycoerythrin PE-SMCC with a specific thioether bond of a sulfhydrylated antibody to obtain a fluorescent labeled antibody; the mixed protein standard substance adopts a freeze-drying protection system containing BSA, trehalose and the like. The single hole of the kit can synchronously detect various cell factors, the sample dosage is only 25 microliters, the kit is suitable for immune monitoring of iron overload and other chronic inflammation models, compared with a traditional ELISA method, the efficiency is improved by 10 times, the cost is reduced, and the kit has the remarkable advantages of being high in throughput, wide in linear range and high in stability.

The invention provides application of high-concentration colloidal gold in preparation of an influenza virus detection test strip, and relates to the technical field of immunodetection. The detection test strip provided by the invention is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, and in the preparation process of the detection test strip, high-concentration colloidal gold with the OD value of 100 is adopted for respectively marking an influenza A virus monoclonal antibody 1, an influenza B virus monoclonal antibody 1 and a chicken IgY antibody; optimized colloidal gold labeled reconstitution fluid is mixed and then sprayed on the combination pad, and meanwhile, a corresponding capture antibody and a goat anti-chicken IgY antibody are respectively sprayed on the nitrocellulose membrane to form a detection line and a quality control line. Through the synergistic effect of the high-concentration colloidal gold mark, the optimized sample pad / combination pad treating fluid and the special sample diluent, the detection sensitivity is remarkably improved, the false negative rate is effectively reduced, and rapid, high-sensitivity and high-specificity field detection on influenza A and B viruses is realized.