Cropping GST protein thermal induction fusion expression plasmid and preparation method thereof

A technology of fusion expression and heat induction, which is applied in the direction of using vectors to introduce foreign genetic material, DNA/RNA fragments, recombinant DNA technology, etc., to save time and workload and reduce identification costs

Inactive Publication Date: 2008-07-09
SHANGHAI INST OF PLANNED PARENTHOOD RES +2
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the biosynthetic peptide epitope identification method is still in the early stage of development, and there are still many ar

Method used

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  • Cropping GST protein thermal induction fusion expression plasmid and preparation method thereof
  • Cropping GST protein thermal induction fusion expression plasmid and preparation method thereof
  • Cropping GST protein thermal induction fusion expression plasmid and preparation method thereof

Examples

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Embodiment Construction

[0046] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and not to limit the scope of the present invention. The experimental method that does not indicate specific condition in the following examples, all according to conventional conditions and the third edition of "Molecular Cloning: Laboratory Handbook" (Science Publishing) translated by Huang Peitang et al. Press, 2002) and [US] E. Harlow and Ryan edited the steps described in "Antibody Technology Experiment Guide" (Science Press, 2002) translated by Shen Care etc., or according to the conditions suggested by the manufacturer and seller .

[0047] Materials and methods

[0048] 1. Heat-inducible expression plasmid pSY621 (Figure 1) and Escherichia coli BL21 (DE3) strain were constructed and preserved by the State Key Laboratory of Genetic Engineering, Fudan University.

[0049] 2. The pG...

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Abstract

The invention belongs to the technical field of biological engineering and in particular is a heat-induced fusion expression recombinant plasmid of truncated GST protein and a process for preparation. The invention selects utility truncated GST188 protein which is composed of 188aa as a carrier, a pXXGST-1 recombinant plasmid which expresses short peptide fusion protein in an escherichia coli heat-induced expression system and a heat-induced type pXXGST-2 recombinant plasmid which is used to express comparison to realize the aim of synthesizing short peptide creatures, which is especially provided for scanning, drawing, positioning antigen linear epitopes and identifying epitope motif. Experiments of the invention prove the adaptability and the applicability of the GST188 core protein which is used as the short peptide creatures expression vector, in antigen epitope scanning identification, and in particular when anti- recombinant target protein antiserum is used to indentify the epitope motif.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to the use of the 26 kDa glutathione-S-transferase (Sj26GST) core protein (composed of 188 amino acids) of Schistosoma japonicum Philippine strain as a short peptide (8-18 peptide) biosynthesis carrier, Escherichia coli heat-induced GST188 recombinant expression plasmids pXXGST-1 and pXXGST-2, which are specially used for antigen epitope mapping and identification and epitope motif identification, and their preparation methods. Background technique [0002] The linear B cell epitope (B cell epitope, BCE) on the protein molecule is the basis of its antigenicity. Detailed mapping of epitope maps of specific antigens and fine identification of their antibody recognition motifs, research on the structure and function of proteins, diagnosis of diseases, site-specific modification of protein molecules to reduce the immunogenicity of protein drugs, and design of synthesis...

Claims

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Application Information

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IPC IPC(8): C12N15/70C12N15/62
Inventor 徐万祥季朝能何亚萍顾少华谢毅唐海平
Owner SHANGHAI INST OF PLANNED PARENTHOOD RES
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