The invention discloses an
optical biosensor for detecting
brain natriuretic peptide (BNP) and a preparation method of a
reagent thereof, relating to the biosensing technology. A sensor
reaction zone is a
capillary channel filled with magnetic nano probe solution, the inner surface of the channel is assembled with a layer of hydrophilic nano material, the upper surface of the channel is an euphotic insulating encapsulation layer with an injection hole, the side surface of the channel is encapsulated by plastics, the two ends thereof encapsulated with plastics are respectively provided with a controllable
electromagnet. The preparation method of the
reagent is as follows: preparing magnetic nano probes, fully reacting the magnetic nano probes, a
brain natriuretic peptide (BNP) sample to bedetected and specificity BNP
antigen marked by a marker under the united action of the
electromagnet and the
capillary channel, forming a compound of magnetic nano probes, BNP
antigen to be detected and marked
antibody, and adding substrate for catalytic
luminescence or exciting luminescent
quantum dots for
luminescence. By the sensor detection
system, the BNP concentration can be rapidly detected. The sensor in the invention can rapidly detect the BNP concentration in a sample, and has the characteristics of high specificity, high sensitivity, a great quantity of saved biological
reagent andthe like.